An in vitro system from Plasmodium falciparum active in endogenous mRNA translation

Detalhes bibliográficos
Autor(a) principal: Ferreras,Ana
Data de Publicação: 2000
Outros Autores: Triana,Ledia, Correia,Heriberto, Sánchez,Erlinda, Herrera,Flor
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Memórias do Instituto Oswaldo Cruz
Texto Completo: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762000000200017
Resumo: An in vitro translation system has been prepared from Plasmodium falciparum by saponin lysis of infected-erythrocytes to free parasites which were homogeneized with glass beads, centrifuged to obtain a S-30 fraction followed by Sephadex G-25 gel filtration. This treatment produced a system with very low contamination of host proteins (<1%). The system, optimized for Mg2+ and K+, translates endogenous mRNA and is active for 80 min which suggests that their protein factors and mRNA are quite stable.
id FIOCRUZ-4_7985e593d8fab2126c0700abf7808812
oai_identifier_str oai:scielo:S0074-02762000000200017
network_acronym_str FIOCRUZ-4
network_name_str Memórias do Instituto Oswaldo Cruz
spelling An in vitro system from Plasmodium falciparum active in endogenous mRNA translationPlasmodium falciparumprotein synthesiscell free translationparasite lysateAn in vitro translation system has been prepared from Plasmodium falciparum by saponin lysis of infected-erythrocytes to free parasites which were homogeneized with glass beads, centrifuged to obtain a S-30 fraction followed by Sephadex G-25 gel filtration. This treatment produced a system with very low contamination of host proteins (<1%). The system, optimized for Mg2+ and K+, translates endogenous mRNA and is active for 80 min which suggests that their protein factors and mRNA are quite stable.Instituto Oswaldo Cruz, Ministério da Saúde2000-04-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762000000200017Memórias do Instituto Oswaldo Cruz v.95 n.2 2000reponame:Memórias do Instituto Oswaldo Cruzinstname:Fundação Oswaldo Cruzinstacron:FIOCRUZ10.1590/S0074-02762000000200017info:eu-repo/semantics/openAccessFerreras,AnaTriana,LediaCorreia,HeribertoSánchez,ErlindaHerrera,Floreng2020-04-25T17:48:21Zhttp://www.scielo.br/oai/scielo-oai.php0074-02761678-8060opendoar:null2020-04-26 02:09:36.512Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruztrue
dc.title.none.fl_str_mv An in vitro system from Plasmodium falciparum active in endogenous mRNA translation
title An in vitro system from Plasmodium falciparum active in endogenous mRNA translation
spellingShingle An in vitro system from Plasmodium falciparum active in endogenous mRNA translation
Ferreras,Ana
Plasmodium falciparum
protein synthesis
cell free translation
parasite lysate
title_short An in vitro system from Plasmodium falciparum active in endogenous mRNA translation
title_full An in vitro system from Plasmodium falciparum active in endogenous mRNA translation
title_fullStr An in vitro system from Plasmodium falciparum active in endogenous mRNA translation
title_full_unstemmed An in vitro system from Plasmodium falciparum active in endogenous mRNA translation
title_sort An in vitro system from Plasmodium falciparum active in endogenous mRNA translation
author Ferreras,Ana
author_facet Ferreras,Ana
Triana,Ledia
Correia,Heriberto
Sánchez,Erlinda
Herrera,Flor
author_role author
author2 Triana,Ledia
Correia,Heriberto
Sánchez,Erlinda
Herrera,Flor
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Ferreras,Ana
Triana,Ledia
Correia,Heriberto
Sánchez,Erlinda
Herrera,Flor
dc.subject.por.fl_str_mv Plasmodium falciparum
protein synthesis
cell free translation
parasite lysate
topic Plasmodium falciparum
protein synthesis
cell free translation
parasite lysate
dc.description.none.fl_txt_mv An in vitro translation system has been prepared from Plasmodium falciparum by saponin lysis of infected-erythrocytes to free parasites which were homogeneized with glass beads, centrifuged to obtain a S-30 fraction followed by Sephadex G-25 gel filtration. This treatment produced a system with very low contamination of host proteins (<1%). The system, optimized for Mg2+ and K+, translates endogenous mRNA and is active for 80 min which suggests that their protein factors and mRNA are quite stable.
description An in vitro translation system has been prepared from Plasmodium falciparum by saponin lysis of infected-erythrocytes to free parasites which were homogeneized with glass beads, centrifuged to obtain a S-30 fraction followed by Sephadex G-25 gel filtration. This treatment produced a system with very low contamination of host proteins (<1%). The system, optimized for Mg2+ and K+, translates endogenous mRNA and is active for 80 min which suggests that their protein factors and mRNA are quite stable.
publishDate 2000
dc.date.none.fl_str_mv 2000-04-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762000000200017
url http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762000000200017
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0074-02762000000200017
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
dc.source.none.fl_str_mv Memórias do Instituto Oswaldo Cruz v.95 n.2 2000
reponame:Memórias do Instituto Oswaldo Cruz
instname:Fundação Oswaldo Cruz
instacron:FIOCRUZ
reponame_str Memórias do Instituto Oswaldo Cruz
collection Memórias do Instituto Oswaldo Cruz
instname_str Fundação Oswaldo Cruz
instacron_str FIOCRUZ
institution FIOCRUZ
repository.name.fl_str_mv Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruz
repository.mail.fl_str_mv
_version_ 1669937679656026112

Registros relacionados