Drug discovery for Chagas disease should consider Trypanosoma cruzi strain diversity
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Memórias do Instituto Oswaldo Cruz |
Texto Completo: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762014000600828 |
Resumo: | This opinion piece presents an approach to standardisation of an important aspect of Chagas disease drug discovery and development: selecting Trypanosoma cruzi strains for in vitro screening. We discuss the rationale for strain selection representing T. cruzi diversity and provide recommendations on the preferred parasite stage for drug discovery, T. cruzi discrete typing units to include in the panel of strains and the number of strains/clones for primary screens and lead compounds. We also consider experimental approaches for in vitro drug assays. The Figure illustrates the current Chagas disease drug-discovery and development landscape. |
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Memórias do Instituto Oswaldo Cruz |
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Drug discovery for Chagas disease should consider Trypanosoma cruzi strain diversityin vitro drug screeningT. cruzigenetic diversityguidelinesThis opinion piece presents an approach to standardisation of an important aspect of Chagas disease drug discovery and development: selecting Trypanosoma cruzi strains for in vitro screening. We discuss the rationale for strain selection representing T. cruzi diversity and provide recommendations on the preferred parasite stage for drug discovery, T. cruzi discrete typing units to include in the panel of strains and the number of strains/clones for primary screens and lead compounds. We also consider experimental approaches for in vitro drug assays. The Figure illustrates the current Chagas disease drug-discovery and development landscape.Instituto Oswaldo Cruz, Ministério da Saúde2014-09-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762014000600828Memórias do Instituto Oswaldo Cruz v.109 n.6 2014reponame:Memórias do Instituto Oswaldo Cruzinstname:Fundação Oswaldo Cruzinstacron:FIOCRUZ10.1590/0074-0276140156info:eu-repo/semantics/openAccessZingales,BiancaMiles,Michael AMoraes,Carolina BLuquetti,AlejandroGuhl,FelipeSchijman,Alejandro GRibeiro,Isabelaeng2020-04-25T17:51:48Zhttp://www.scielo.br/oai/scielo-oai.php0074-02761678-8060opendoar:null2020-04-26 02:19:50.662Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruztrue |
dc.title.none.fl_str_mv |
Drug discovery for Chagas disease should consider Trypanosoma cruzi strain diversity |
title |
Drug discovery for Chagas disease should consider Trypanosoma cruzi strain diversity |
spellingShingle |
Drug discovery for Chagas disease should consider Trypanosoma cruzi strain diversity Zingales,Bianca in vitro drug screening T. cruzi genetic diversity guidelines |
title_short |
Drug discovery for Chagas disease should consider Trypanosoma cruzi strain diversity |
title_full |
Drug discovery for Chagas disease should consider Trypanosoma cruzi strain diversity |
title_fullStr |
Drug discovery for Chagas disease should consider Trypanosoma cruzi strain diversity |
title_full_unstemmed |
Drug discovery for Chagas disease should consider Trypanosoma cruzi strain diversity |
title_sort |
Drug discovery for Chagas disease should consider Trypanosoma cruzi strain diversity |
author |
Zingales,Bianca |
author_facet |
Zingales,Bianca Miles,Michael A Moraes,Carolina B Luquetti,Alejandro Guhl,Felipe Schijman,Alejandro G Ribeiro,Isabela |
author_role |
author |
author2 |
Miles,Michael A Moraes,Carolina B Luquetti,Alejandro Guhl,Felipe Schijman,Alejandro G Ribeiro,Isabela |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Zingales,Bianca Miles,Michael A Moraes,Carolina B Luquetti,Alejandro Guhl,Felipe Schijman,Alejandro G Ribeiro,Isabela |
dc.subject.por.fl_str_mv |
in vitro drug screening T. cruzi genetic diversity guidelines |
topic |
in vitro drug screening T. cruzi genetic diversity guidelines |
dc.description.none.fl_txt_mv |
This opinion piece presents an approach to standardisation of an important aspect of Chagas disease drug discovery and development: selecting Trypanosoma cruzi strains for in vitro screening. We discuss the rationale for strain selection representing T. cruzi diversity and provide recommendations on the preferred parasite stage for drug discovery, T. cruzi discrete typing units to include in the panel of strains and the number of strains/clones for primary screens and lead compounds. We also consider experimental approaches for in vitro drug assays. The Figure illustrates the current Chagas disease drug-discovery and development landscape. |
description |
This opinion piece presents an approach to standardisation of an important aspect of Chagas disease drug discovery and development: selecting Trypanosoma cruzi strains for in vitro screening. We discuss the rationale for strain selection representing T. cruzi diversity and provide recommendations on the preferred parasite stage for drug discovery, T. cruzi discrete typing units to include in the panel of strains and the number of strains/clones for primary screens and lead compounds. We also consider experimental approaches for in vitro drug assays. The Figure illustrates the current Chagas disease drug-discovery and development landscape. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-09-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762014000600828 |
url |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762014000600828 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/0074-0276140156 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Instituto Oswaldo Cruz, Ministério da Saúde |
publisher.none.fl_str_mv |
Instituto Oswaldo Cruz, Ministério da Saúde |
dc.source.none.fl_str_mv |
Memórias do Instituto Oswaldo Cruz v.109 n.6 2014 reponame:Memórias do Instituto Oswaldo Cruz instname:Fundação Oswaldo Cruz instacron:FIOCRUZ |
reponame_str |
Memórias do Instituto Oswaldo Cruz |
collection |
Memórias do Instituto Oswaldo Cruz |
instname_str |
Fundação Oswaldo Cruz |
instacron_str |
FIOCRUZ |
institution |
FIOCRUZ |
repository.name.fl_str_mv |
Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruz |
repository.mail.fl_str_mv |
|
_version_ |
1669937716119207936 |