Diagnostic challenges of single plaque-like lesion paucibacillary leprosy

Detalhes bibliográficos
Autor(a) principal: Barbieri,Raquel Rodrigues
Data de Publicação: 2014
Outros Autores: Sales,Anna Maria, Illarramendi,Ximena, Moraes,Milton Ozório, Nery,José Augusto da Costa, Moreira,Suelen Justo Maria, Sarno,Euzenir Nunes, Machado,Alice de Miranda, Bozza,Fernando Augusto
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Memórias do Instituto Oswaldo Cruz
Texto Completo: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762014000700944
Resumo: The diagnosis of single-lesion paucibacillary leprosy remains a challenge. Reviews by expert dermatopathologists and quantitative polymerase chain reaction (qPCR) results obtained from 66 single-plaque biopsy samples were compared. Histological findings were graded as high (HP), medium (MP) or low (LP) probability of leprosy or other dermatopathy (OD). Mycobacterium leprae-specific genes were detected using qPCR. The biopsies of 47 out of 57 clinically diagnosed patients who received multidrug therapy were classified as HP/MP, eight of which were qPCR negative. In the LP/OD (n = 19), two out of eight untreated patients showed positive qPCR results. In the absence of typical histopathological features, qPCR may be utilised to aid in final patient diagnosis, thus reducing overtreatment and delay in diagnosis.
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spelling Diagnostic challenges of single plaque-like lesion paucibacillary leprosypaucibacillary leprosydiagnosisquantitative polymerase chain reactionThe diagnosis of single-lesion paucibacillary leprosy remains a challenge. Reviews by expert dermatopathologists and quantitative polymerase chain reaction (qPCR) results obtained from 66 single-plaque biopsy samples were compared. Histological findings were graded as high (HP), medium (MP) or low (LP) probability of leprosy or other dermatopathy (OD). Mycobacterium leprae-specific genes were detected using qPCR. The biopsies of 47 out of 57 clinically diagnosed patients who received multidrug therapy were classified as HP/MP, eight of which were qPCR negative. In the LP/OD (n = 19), two out of eight untreated patients showed positive qPCR results. In the absence of typical histopathological features, qPCR may be utilised to aid in final patient diagnosis, thus reducing overtreatment and delay in diagnosis.Instituto Oswaldo Cruz, Ministério da Saúde2014-11-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762014000700944Memórias do Instituto Oswaldo Cruz v.109 n.7 2014reponame:Memórias do Instituto Oswaldo Cruzinstname:Fundação Oswaldo Cruzinstacron:FIOCRUZ10.1590/0074-0276140212info:eu-repo/semantics/openAccessBarbieri,Raquel RodriguesSales,Anna MariaIllarramendi,XimenaMoraes,Milton OzórioNery,José Augusto da CostaMoreira,Suelen Justo MariaSarno,Euzenir NunesMachado,Alice de MirandaBozza,Fernando Augustoeng2020-04-25T17:51:48Zhttp://www.scielo.br/oai/scielo-oai.php0074-02761678-8060opendoar:null2020-04-26 02:19:54.031Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruztrue
dc.title.none.fl_str_mv Diagnostic challenges of single plaque-like lesion paucibacillary leprosy
title Diagnostic challenges of single plaque-like lesion paucibacillary leprosy
spellingShingle Diagnostic challenges of single plaque-like lesion paucibacillary leprosy
Barbieri,Raquel Rodrigues
paucibacillary leprosy
diagnosis
quantitative polymerase chain reaction
title_short Diagnostic challenges of single plaque-like lesion paucibacillary leprosy
title_full Diagnostic challenges of single plaque-like lesion paucibacillary leprosy
title_fullStr Diagnostic challenges of single plaque-like lesion paucibacillary leprosy
title_full_unstemmed Diagnostic challenges of single plaque-like lesion paucibacillary leprosy
title_sort Diagnostic challenges of single plaque-like lesion paucibacillary leprosy
author Barbieri,Raquel Rodrigues
author_facet Barbieri,Raquel Rodrigues
Sales,Anna Maria
Illarramendi,Ximena
Moraes,Milton Ozório
Nery,José Augusto da Costa
Moreira,Suelen Justo Maria
Sarno,Euzenir Nunes
Machado,Alice de Miranda
Bozza,Fernando Augusto
author_role author
author2 Sales,Anna Maria
Illarramendi,Ximena
Moraes,Milton Ozório
Nery,José Augusto da Costa
Moreira,Suelen Justo Maria
Sarno,Euzenir Nunes
Machado,Alice de Miranda
Bozza,Fernando Augusto
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Barbieri,Raquel Rodrigues
Sales,Anna Maria
Illarramendi,Ximena
Moraes,Milton Ozório
Nery,José Augusto da Costa
Moreira,Suelen Justo Maria
Sarno,Euzenir Nunes
Machado,Alice de Miranda
Bozza,Fernando Augusto
dc.subject.por.fl_str_mv paucibacillary leprosy
diagnosis
quantitative polymerase chain reaction
topic paucibacillary leprosy
diagnosis
quantitative polymerase chain reaction
dc.description.none.fl_txt_mv The diagnosis of single-lesion paucibacillary leprosy remains a challenge. Reviews by expert dermatopathologists and quantitative polymerase chain reaction (qPCR) results obtained from 66 single-plaque biopsy samples were compared. Histological findings were graded as high (HP), medium (MP) or low (LP) probability of leprosy or other dermatopathy (OD). Mycobacterium leprae-specific genes were detected using qPCR. The biopsies of 47 out of 57 clinically diagnosed patients who received multidrug therapy were classified as HP/MP, eight of which were qPCR negative. In the LP/OD (n = 19), two out of eight untreated patients showed positive qPCR results. In the absence of typical histopathological features, qPCR may be utilised to aid in final patient diagnosis, thus reducing overtreatment and delay in diagnosis.
description The diagnosis of single-lesion paucibacillary leprosy remains a challenge. Reviews by expert dermatopathologists and quantitative polymerase chain reaction (qPCR) results obtained from 66 single-plaque biopsy samples were compared. Histological findings were graded as high (HP), medium (MP) or low (LP) probability of leprosy or other dermatopathy (OD). Mycobacterium leprae-specific genes were detected using qPCR. The biopsies of 47 out of 57 clinically diagnosed patients who received multidrug therapy were classified as HP/MP, eight of which were qPCR negative. In the LP/OD (n = 19), two out of eight untreated patients showed positive qPCR results. In the absence of typical histopathological features, qPCR may be utilised to aid in final patient diagnosis, thus reducing overtreatment and delay in diagnosis.
publishDate 2014
dc.date.none.fl_str_mv 2014-11-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762014000700944
url http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762014000700944
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/0074-0276140212
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
dc.source.none.fl_str_mv Memórias do Instituto Oswaldo Cruz v.109 n.7 2014
reponame:Memórias do Instituto Oswaldo Cruz
instname:Fundação Oswaldo Cruz
instacron:FIOCRUZ
reponame_str Memórias do Instituto Oswaldo Cruz
collection Memórias do Instituto Oswaldo Cruz
instname_str Fundação Oswaldo Cruz
instacron_str FIOCRUZ
institution FIOCRUZ
repository.name.fl_str_mv Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruz
repository.mail.fl_str_mv
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