Diagnostic challenges of single plaque-like lesion paucibacillary leprosy
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Memórias do Instituto Oswaldo Cruz |
Texto Completo: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762014000700944 |
Resumo: | The diagnosis of single-lesion paucibacillary leprosy remains a challenge. Reviews by expert dermatopathologists and quantitative polymerase chain reaction (qPCR) results obtained from 66 single-plaque biopsy samples were compared. Histological findings were graded as high (HP), medium (MP) or low (LP) probability of leprosy or other dermatopathy (OD). Mycobacterium leprae-specific genes were detected using qPCR. The biopsies of 47 out of 57 clinically diagnosed patients who received multidrug therapy were classified as HP/MP, eight of which were qPCR negative. In the LP/OD (n = 19), two out of eight untreated patients showed positive qPCR results. In the absence of typical histopathological features, qPCR may be utilised to aid in final patient diagnosis, thus reducing overtreatment and delay in diagnosis. |
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Memórias do Instituto Oswaldo Cruz |
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Diagnostic challenges of single plaque-like lesion paucibacillary leprosypaucibacillary leprosydiagnosisquantitative polymerase chain reactionThe diagnosis of single-lesion paucibacillary leprosy remains a challenge. Reviews by expert dermatopathologists and quantitative polymerase chain reaction (qPCR) results obtained from 66 single-plaque biopsy samples were compared. Histological findings were graded as high (HP), medium (MP) or low (LP) probability of leprosy or other dermatopathy (OD). Mycobacterium leprae-specific genes were detected using qPCR. The biopsies of 47 out of 57 clinically diagnosed patients who received multidrug therapy were classified as HP/MP, eight of which were qPCR negative. In the LP/OD (n = 19), two out of eight untreated patients showed positive qPCR results. In the absence of typical histopathological features, qPCR may be utilised to aid in final patient diagnosis, thus reducing overtreatment and delay in diagnosis.Instituto Oswaldo Cruz, Ministério da Saúde2014-11-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762014000700944Memórias do Instituto Oswaldo Cruz v.109 n.7 2014reponame:Memórias do Instituto Oswaldo Cruzinstname:Fundação Oswaldo Cruzinstacron:FIOCRUZ10.1590/0074-0276140212info:eu-repo/semantics/openAccessBarbieri,Raquel RodriguesSales,Anna MariaIllarramendi,XimenaMoraes,Milton OzórioNery,José Augusto da CostaMoreira,Suelen Justo MariaSarno,Euzenir NunesMachado,Alice de MirandaBozza,Fernando Augustoeng2020-04-25T17:51:48Zhttp://www.scielo.br/oai/scielo-oai.php0074-02761678-8060opendoar:null2020-04-26 02:19:54.031Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruztrue |
dc.title.none.fl_str_mv |
Diagnostic challenges of single plaque-like lesion paucibacillary leprosy |
title |
Diagnostic challenges of single plaque-like lesion paucibacillary leprosy |
spellingShingle |
Diagnostic challenges of single plaque-like lesion paucibacillary leprosy Barbieri,Raquel Rodrigues paucibacillary leprosy diagnosis quantitative polymerase chain reaction |
title_short |
Diagnostic challenges of single plaque-like lesion paucibacillary leprosy |
title_full |
Diagnostic challenges of single plaque-like lesion paucibacillary leprosy |
title_fullStr |
Diagnostic challenges of single plaque-like lesion paucibacillary leprosy |
title_full_unstemmed |
Diagnostic challenges of single plaque-like lesion paucibacillary leprosy |
title_sort |
Diagnostic challenges of single plaque-like lesion paucibacillary leprosy |
author |
Barbieri,Raquel Rodrigues |
author_facet |
Barbieri,Raquel Rodrigues Sales,Anna Maria Illarramendi,Ximena Moraes,Milton Ozório Nery,José Augusto da Costa Moreira,Suelen Justo Maria Sarno,Euzenir Nunes Machado,Alice de Miranda Bozza,Fernando Augusto |
author_role |
author |
author2 |
Sales,Anna Maria Illarramendi,Ximena Moraes,Milton Ozório Nery,José Augusto da Costa Moreira,Suelen Justo Maria Sarno,Euzenir Nunes Machado,Alice de Miranda Bozza,Fernando Augusto |
author2_role |
author author author author author author author author |
dc.contributor.author.fl_str_mv |
Barbieri,Raquel Rodrigues Sales,Anna Maria Illarramendi,Ximena Moraes,Milton Ozório Nery,José Augusto da Costa Moreira,Suelen Justo Maria Sarno,Euzenir Nunes Machado,Alice de Miranda Bozza,Fernando Augusto |
dc.subject.por.fl_str_mv |
paucibacillary leprosy diagnosis quantitative polymerase chain reaction |
topic |
paucibacillary leprosy diagnosis quantitative polymerase chain reaction |
dc.description.none.fl_txt_mv |
The diagnosis of single-lesion paucibacillary leprosy remains a challenge. Reviews by expert dermatopathologists and quantitative polymerase chain reaction (qPCR) results obtained from 66 single-plaque biopsy samples were compared. Histological findings were graded as high (HP), medium (MP) or low (LP) probability of leprosy or other dermatopathy (OD). Mycobacterium leprae-specific genes were detected using qPCR. The biopsies of 47 out of 57 clinically diagnosed patients who received multidrug therapy were classified as HP/MP, eight of which were qPCR negative. In the LP/OD (n = 19), two out of eight untreated patients showed positive qPCR results. In the absence of typical histopathological features, qPCR may be utilised to aid in final patient diagnosis, thus reducing overtreatment and delay in diagnosis. |
description |
The diagnosis of single-lesion paucibacillary leprosy remains a challenge. Reviews by expert dermatopathologists and quantitative polymerase chain reaction (qPCR) results obtained from 66 single-plaque biopsy samples were compared. Histological findings were graded as high (HP), medium (MP) or low (LP) probability of leprosy or other dermatopathy (OD). Mycobacterium leprae-specific genes were detected using qPCR. The biopsies of 47 out of 57 clinically diagnosed patients who received multidrug therapy were classified as HP/MP, eight of which were qPCR negative. In the LP/OD (n = 19), two out of eight untreated patients showed positive qPCR results. In the absence of typical histopathological features, qPCR may be utilised to aid in final patient diagnosis, thus reducing overtreatment and delay in diagnosis. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-11-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762014000700944 |
url |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762014000700944 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/0074-0276140212 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Instituto Oswaldo Cruz, Ministério da Saúde |
publisher.none.fl_str_mv |
Instituto Oswaldo Cruz, Ministério da Saúde |
dc.source.none.fl_str_mv |
Memórias do Instituto Oswaldo Cruz v.109 n.7 2014 reponame:Memórias do Instituto Oswaldo Cruz instname:Fundação Oswaldo Cruz instacron:FIOCRUZ |
reponame_str |
Memórias do Instituto Oswaldo Cruz |
collection |
Memórias do Instituto Oswaldo Cruz |
instname_str |
Fundação Oswaldo Cruz |
instacron_str |
FIOCRUZ |
institution |
FIOCRUZ |
repository.name.fl_str_mv |
Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruz |
repository.mail.fl_str_mv |
|
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1669937716966457344 |