Differential tissue tropism of Trypanosoma cruzi strains: an in vitro study

Detalhes bibliográficos
Autor(a) principal: Andrade,Luciana O
Data de Publicação: 2010
Outros Autores: Galvão,Lúcia MC, Meirelles,Maria de Nazareth SL, Chiari,Egler, Pena,Sergio DJ, Macedo,Andrea M
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Memórias do Instituto Oswaldo Cruz
Texto Completo: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762010000600018
Resumo: We have previously demonstrated selection favoring the JG strain of Trypanosoma cruziin hearts of BALB/c mice that were chronically infected with an equal mixture of the monoclonal JG strain and a clone of the Colombian strain, Col1.7G2. To evaluate whether cell invasion efficiency drives this selection, we infected primary cultures of BALB/c cardiomyocytes using these same T. cruzi populations. Contrary to expectation, Col1.7G2 parasites invaded heart cell cultures in higher numbers than JG parasites; however, intracellular multiplication of JG parasites was more efficient than that of Col1.7G2 parasites. This phenomenon was only observed for cardiomyocytes and not for cultured Vero cells. Double infections (Col1.7G2 + JG) showed similar results. Even though invasion might influence tissue selection, our data strongly suggest that intracellular development is important to determine parasite tissue tropism.
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spelling Differential tissue tropism of Trypanosoma cruzi strains: an in vitro studyTrypanosoma cruziChagas diseasetissue tropismcardiomyocytesWe have previously demonstrated selection favoring the JG strain of Trypanosoma cruziin hearts of BALB/c mice that were chronically infected with an equal mixture of the monoclonal JG strain and a clone of the Colombian strain, Col1.7G2. To evaluate whether cell invasion efficiency drives this selection, we infected primary cultures of BALB/c cardiomyocytes using these same T. cruzi populations. Contrary to expectation, Col1.7G2 parasites invaded heart cell cultures in higher numbers than JG parasites; however, intracellular multiplication of JG parasites was more efficient than that of Col1.7G2 parasites. This phenomenon was only observed for cardiomyocytes and not for cultured Vero cells. Double infections (Col1.7G2 + JG) showed similar results. Even though invasion might influence tissue selection, our data strongly suggest that intracellular development is important to determine parasite tissue tropism.Instituto Oswaldo Cruz, Ministério da Saúde2010-09-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762010000600018Memórias do Instituto Oswaldo Cruz v.105 n.6 2010reponame:Memórias do Instituto Oswaldo Cruzinstname:Fundação Oswaldo Cruzinstacron:FIOCRUZ10.1590/S0074-02762010000600018info:eu-repo/semantics/openAccessAndrade,Luciana OGalvão,Lúcia MCMeirelles,Maria de Nazareth SLChiari,EglerPena,Sergio DJMacedo,Andrea Meng2020-04-25T17:50:52Zhttp://www.scielo.br/oai/scielo-oai.php0074-02761678-8060opendoar:null2020-04-26 02:17:16.434Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruztrue
dc.title.none.fl_str_mv Differential tissue tropism of Trypanosoma cruzi strains: an in vitro study
title Differential tissue tropism of Trypanosoma cruzi strains: an in vitro study
spellingShingle Differential tissue tropism of Trypanosoma cruzi strains: an in vitro study
Andrade,Luciana O
Trypanosoma cruzi
Chagas disease
tissue tropism
cardiomyocytes
title_short Differential tissue tropism of Trypanosoma cruzi strains: an in vitro study
title_full Differential tissue tropism of Trypanosoma cruzi strains: an in vitro study
title_fullStr Differential tissue tropism of Trypanosoma cruzi strains: an in vitro study
title_full_unstemmed Differential tissue tropism of Trypanosoma cruzi strains: an in vitro study
title_sort Differential tissue tropism of Trypanosoma cruzi strains: an in vitro study
author Andrade,Luciana O
author_facet Andrade,Luciana O
Galvão,Lúcia MC
Meirelles,Maria de Nazareth SL
Chiari,Egler
Pena,Sergio DJ
Macedo,Andrea M
author_role author
author2 Galvão,Lúcia MC
Meirelles,Maria de Nazareth SL
Chiari,Egler
Pena,Sergio DJ
Macedo,Andrea M
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Andrade,Luciana O
Galvão,Lúcia MC
Meirelles,Maria de Nazareth SL
Chiari,Egler
Pena,Sergio DJ
Macedo,Andrea M
dc.subject.por.fl_str_mv Trypanosoma cruzi
Chagas disease
tissue tropism
cardiomyocytes
topic Trypanosoma cruzi
Chagas disease
tissue tropism
cardiomyocytes
dc.description.none.fl_txt_mv We have previously demonstrated selection favoring the JG strain of Trypanosoma cruziin hearts of BALB/c mice that were chronically infected with an equal mixture of the monoclonal JG strain and a clone of the Colombian strain, Col1.7G2. To evaluate whether cell invasion efficiency drives this selection, we infected primary cultures of BALB/c cardiomyocytes using these same T. cruzi populations. Contrary to expectation, Col1.7G2 parasites invaded heart cell cultures in higher numbers than JG parasites; however, intracellular multiplication of JG parasites was more efficient than that of Col1.7G2 parasites. This phenomenon was only observed for cardiomyocytes and not for cultured Vero cells. Double infections (Col1.7G2 + JG) showed similar results. Even though invasion might influence tissue selection, our data strongly suggest that intracellular development is important to determine parasite tissue tropism.
description We have previously demonstrated selection favoring the JG strain of Trypanosoma cruziin hearts of BALB/c mice that were chronically infected with an equal mixture of the monoclonal JG strain and a clone of the Colombian strain, Col1.7G2. To evaluate whether cell invasion efficiency drives this selection, we infected primary cultures of BALB/c cardiomyocytes using these same T. cruzi populations. Contrary to expectation, Col1.7G2 parasites invaded heart cell cultures in higher numbers than JG parasites; however, intracellular multiplication of JG parasites was more efficient than that of Col1.7G2 parasites. This phenomenon was only observed for cardiomyocytes and not for cultured Vero cells. Double infections (Col1.7G2 + JG) showed similar results. Even though invasion might influence tissue selection, our data strongly suggest that intracellular development is important to determine parasite tissue tropism.
publishDate 2010
dc.date.none.fl_str_mv 2010-09-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762010000600018
url http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762010000600018
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0074-02762010000600018
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
dc.source.none.fl_str_mv Memórias do Instituto Oswaldo Cruz v.105 n.6 2010
reponame:Memórias do Instituto Oswaldo Cruz
instname:Fundação Oswaldo Cruz
instacron:FIOCRUZ
reponame_str Memórias do Instituto Oswaldo Cruz
collection Memórias do Instituto Oswaldo Cruz
instname_str Fundação Oswaldo Cruz
instacron_str FIOCRUZ
institution FIOCRUZ
repository.name.fl_str_mv Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruz
repository.mail.fl_str_mv
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