Red blood cells derived from peripheral blood and bone marrow CD34+ human haematopoietic stem cells are permissive to Plasmodium parasites infection

Detalhes bibliográficos
Autor(a) principal: Fernandez-Becerra,Carmen
Data de Publicação: 2013
Outros Autores: Lelievre,Joel, Ferrer,Mireia, Anton,Nuria, Thomson,Richard, Peligero,Cristina, Almela,Maria Jesus, Lacerda,Marcus VG, Herreros,Esperanza, del Portillo,Hernando A
Tipo de documento: Relatório
Idioma: eng
Título da fonte: Memórias do Instituto Oswaldo Cruz
Texto Completo: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762013000600801
Resumo: The production of fully functional human red cells in vitro from haematopoietic stem cells (hHSCs) has been successfully achieved. Recently, the use of hHSCs from cord blood represented a major improvement to develop the continuous culture system for Plasmodium vivax. Here, we demonstrated that CD34+hHSCs from peripheral blood and bone marrow can be expanded and differentiated to reticulocytes using a novel stromal cell. Moreover, these reticulocytes and mature red blood cells express surface markers for entrance of malaria parasites contain adult haemoglobin and are also permissive to invasion by P. vivax and Plasmodium falciparum parasites.
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spelling Red blood cells derived from peripheral blood and bone marrow CD34+ human haematopoietic stem cells are permissive to Plasmodium parasites infectionCD34+ human haematopoietic stem cellsNIH 3T3 cellsPlasmodiumThe production of fully functional human red cells in vitro from haematopoietic stem cells (hHSCs) has been successfully achieved. Recently, the use of hHSCs from cord blood represented a major improvement to develop the continuous culture system for Plasmodium vivax. Here, we demonstrated that CD34+hHSCs from peripheral blood and bone marrow can be expanded and differentiated to reticulocytes using a novel stromal cell. Moreover, these reticulocytes and mature red blood cells express surface markers for entrance of malaria parasites contain adult haemoglobin and are also permissive to invasion by P. vivax and Plasmodium falciparum parasites.Instituto Oswaldo Cruz, Ministério da Saúde2013-09-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/reporttext/htmlhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762013000600801Memórias do Instituto Oswaldo Cruz v.108 n.6 2013reponame:Memórias do Instituto Oswaldo Cruzinstname:Fundação Oswaldo Cruzinstacron:FIOCRUZ10.1590/0074-0276108062013019info:eu-repo/semantics/openAccessFernandez-Becerra,CarmenLelievre,JoelFerrer,MireiaAnton,NuriaThomson,RichardPeligero,CristinaAlmela,Maria JesusLacerda,Marcus VGHerreros,Esperanzadel Portillo,Hernando Aeng2020-04-25T17:51:34Zhttp://www.scielo.br/oai/scielo-oai.php0074-02761678-8060opendoar:null2020-04-26 02:19:11.878Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruztrue
dc.title.none.fl_str_mv Red blood cells derived from peripheral blood and bone marrow CD34+ human haematopoietic stem cells are permissive to Plasmodium parasites infection
title Red blood cells derived from peripheral blood and bone marrow CD34+ human haematopoietic stem cells are permissive to Plasmodium parasites infection
spellingShingle Red blood cells derived from peripheral blood and bone marrow CD34+ human haematopoietic stem cells are permissive to Plasmodium parasites infection
Fernandez-Becerra,Carmen
CD34+ human haematopoietic stem cells
NIH 3T3 cells
Plasmodium
title_short Red blood cells derived from peripheral blood and bone marrow CD34+ human haematopoietic stem cells are permissive to Plasmodium parasites infection
title_full Red blood cells derived from peripheral blood and bone marrow CD34+ human haematopoietic stem cells are permissive to Plasmodium parasites infection
title_fullStr Red blood cells derived from peripheral blood and bone marrow CD34+ human haematopoietic stem cells are permissive to Plasmodium parasites infection
title_full_unstemmed Red blood cells derived from peripheral blood and bone marrow CD34+ human haematopoietic stem cells are permissive to Plasmodium parasites infection
title_sort Red blood cells derived from peripheral blood and bone marrow CD34+ human haematopoietic stem cells are permissive to Plasmodium parasites infection
author Fernandez-Becerra,Carmen
author_facet Fernandez-Becerra,Carmen
Lelievre,Joel
Ferrer,Mireia
Anton,Nuria
Thomson,Richard
Peligero,Cristina
Almela,Maria Jesus
Lacerda,Marcus VG
Herreros,Esperanza
del Portillo,Hernando A
author_role author
author2 Lelievre,Joel
Ferrer,Mireia
Anton,Nuria
Thomson,Richard
Peligero,Cristina
Almela,Maria Jesus
Lacerda,Marcus VG
Herreros,Esperanza
del Portillo,Hernando A
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Fernandez-Becerra,Carmen
Lelievre,Joel
Ferrer,Mireia
Anton,Nuria
Thomson,Richard
Peligero,Cristina
Almela,Maria Jesus
Lacerda,Marcus VG
Herreros,Esperanza
del Portillo,Hernando A
dc.subject.por.fl_str_mv CD34+ human haematopoietic stem cells
NIH 3T3 cells
Plasmodium
topic CD34+ human haematopoietic stem cells
NIH 3T3 cells
Plasmodium
dc.description.none.fl_txt_mv The production of fully functional human red cells in vitro from haematopoietic stem cells (hHSCs) has been successfully achieved. Recently, the use of hHSCs from cord blood represented a major improvement to develop the continuous culture system for Plasmodium vivax. Here, we demonstrated that CD34+hHSCs from peripheral blood and bone marrow can be expanded and differentiated to reticulocytes using a novel stromal cell. Moreover, these reticulocytes and mature red blood cells express surface markers for entrance of malaria parasites contain adult haemoglobin and are also permissive to invasion by P. vivax and Plasmodium falciparum parasites.
description The production of fully functional human red cells in vitro from haematopoietic stem cells (hHSCs) has been successfully achieved. Recently, the use of hHSCs from cord blood represented a major improvement to develop the continuous culture system for Plasmodium vivax. Here, we demonstrated that CD34+hHSCs from peripheral blood and bone marrow can be expanded and differentiated to reticulocytes using a novel stromal cell. Moreover, these reticulocytes and mature red blood cells express surface markers for entrance of malaria parasites contain adult haemoglobin and are also permissive to invasion by P. vivax and Plasmodium falciparum parasites.
publishDate 2013
dc.date.none.fl_str_mv 2013-09-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/report
status_str publishedVersion
format report
dc.identifier.uri.fl_str_mv http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762013000600801
url http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762013000600801
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/0074-0276108062013019
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
dc.source.none.fl_str_mv Memórias do Instituto Oswaldo Cruz v.108 n.6 2013
reponame:Memórias do Instituto Oswaldo Cruz
instname:Fundação Oswaldo Cruz
instacron:FIOCRUZ
reponame_str Memórias do Instituto Oswaldo Cruz
collection Memórias do Instituto Oswaldo Cruz
instname_str Fundação Oswaldo Cruz
instacron_str FIOCRUZ
institution FIOCRUZ
repository.name.fl_str_mv Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruz
repository.mail.fl_str_mv
_version_ 1669937713987452928

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