Vaccinia virus regulates expression of p21WAF1/Cip1 in A431 cells

Detalhes bibliográficos
Autor(a) principal: Andrade,Anderson A
Data de Publicação: 2010
Outros Autores: Brasil,Bruno SAF, Pereira,Anna CTC, Ferreira,Paulo CP, Kroon,Erna G, Bonjardim,Cláudio A
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Memórias do Instituto Oswaldo Cruz
Texto Completo: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762010000300005
Resumo: In this paper, we provide evidence that both the mRNA and protein levels of the cyclin-dependent kinase (CDK) inhibitor p21WAF1/CDK-interacting protein 1 (Cip1) increase upon infection of A431 cells with Vaccinia virus (VACV). In addition, the VACV growth factor (VGF) seems to be required for the gene expression because infection carried out with the mutant virus VACV-VGF- revealed that this strain was unable to stimulate its transcription. Our findings are also consistent with the notion that the VGF-mediated change in p21WAF1/Cip1 expression is dependent on tyrosine kinase pathway(s) and is partially dependent on mitogen-activated protein kinase/extracellular-signal regulated kinase 1/2. We believe that these pathways are biologically significant because VACV replication and dissemination was drastically affected when the infection was carried out in the presence of the relevant pharmacological inhibitors.
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spelling Vaccinia virus regulates expression of p21WAF1/Cip1 in A431 cellsVaccinia virusvirus-host cell interactionp21WAF1/Cip1MEK/ERK1/2signal transductionIn this paper, we provide evidence that both the mRNA and protein levels of the cyclin-dependent kinase (CDK) inhibitor p21WAF1/CDK-interacting protein 1 (Cip1) increase upon infection of A431 cells with Vaccinia virus (VACV). In addition, the VACV growth factor (VGF) seems to be required for the gene expression because infection carried out with the mutant virus VACV-VGF- revealed that this strain was unable to stimulate its transcription. Our findings are also consistent with the notion that the VGF-mediated change in p21WAF1/Cip1 expression is dependent on tyrosine kinase pathway(s) and is partially dependent on mitogen-activated protein kinase/extracellular-signal regulated kinase 1/2. We believe that these pathways are biologically significant because VACV replication and dissemination was drastically affected when the infection was carried out in the presence of the relevant pharmacological inhibitors.Instituto Oswaldo Cruz, Ministério da Saúde2010-05-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762010000300005Memórias do Instituto Oswaldo Cruz v.105 n.3 2010reponame:Memórias do Instituto Oswaldo Cruzinstname:Fundação Oswaldo Cruzinstacron:FIOCRUZ10.1590/S0074-02762010000300005info:eu-repo/semantics/openAccessAndrade,Anderson ABrasil,Bruno SAFPereira,Anna CTCFerreira,Paulo CPKroon,Erna GBonjardim,Cláudio Aeng2020-04-25T17:50:47Zhttp://www.scielo.br/oai/scielo-oai.php0074-02761678-8060opendoar:null2020-04-26 02:16:53.782Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruztrue
dc.title.none.fl_str_mv Vaccinia virus regulates expression of p21WAF1/Cip1 in A431 cells
title Vaccinia virus regulates expression of p21WAF1/Cip1 in A431 cells
spellingShingle Vaccinia virus regulates expression of p21WAF1/Cip1 in A431 cells
Andrade,Anderson A
Vaccinia virus
virus-host cell interaction
p21WAF1/Cip1
MEK/ERK1/2
signal transduction
title_short Vaccinia virus regulates expression of p21WAF1/Cip1 in A431 cells
title_full Vaccinia virus regulates expression of p21WAF1/Cip1 in A431 cells
title_fullStr Vaccinia virus regulates expression of p21WAF1/Cip1 in A431 cells
title_full_unstemmed Vaccinia virus regulates expression of p21WAF1/Cip1 in A431 cells
title_sort Vaccinia virus regulates expression of p21WAF1/Cip1 in A431 cells
author Andrade,Anderson A
author_facet Andrade,Anderson A
Brasil,Bruno SAF
Pereira,Anna CTC
Ferreira,Paulo CP
Kroon,Erna G
Bonjardim,Cláudio A
author_role author
author2 Brasil,Bruno SAF
Pereira,Anna CTC
Ferreira,Paulo CP
Kroon,Erna G
Bonjardim,Cláudio A
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Andrade,Anderson A
Brasil,Bruno SAF
Pereira,Anna CTC
Ferreira,Paulo CP
Kroon,Erna G
Bonjardim,Cláudio A
dc.subject.por.fl_str_mv Vaccinia virus
virus-host cell interaction
p21WAF1/Cip1
MEK/ERK1/2
signal transduction
topic Vaccinia virus
virus-host cell interaction
p21WAF1/Cip1
MEK/ERK1/2
signal transduction
dc.description.none.fl_txt_mv In this paper, we provide evidence that both the mRNA and protein levels of the cyclin-dependent kinase (CDK) inhibitor p21WAF1/CDK-interacting protein 1 (Cip1) increase upon infection of A431 cells with Vaccinia virus (VACV). In addition, the VACV growth factor (VGF) seems to be required for the gene expression because infection carried out with the mutant virus VACV-VGF- revealed that this strain was unable to stimulate its transcription. Our findings are also consistent with the notion that the VGF-mediated change in p21WAF1/Cip1 expression is dependent on tyrosine kinase pathway(s) and is partially dependent on mitogen-activated protein kinase/extracellular-signal regulated kinase 1/2. We believe that these pathways are biologically significant because VACV replication and dissemination was drastically affected when the infection was carried out in the presence of the relevant pharmacological inhibitors.
description In this paper, we provide evidence that both the mRNA and protein levels of the cyclin-dependent kinase (CDK) inhibitor p21WAF1/CDK-interacting protein 1 (Cip1) increase upon infection of A431 cells with Vaccinia virus (VACV). In addition, the VACV growth factor (VGF) seems to be required for the gene expression because infection carried out with the mutant virus VACV-VGF- revealed that this strain was unable to stimulate its transcription. Our findings are also consistent with the notion that the VGF-mediated change in p21WAF1/Cip1 expression is dependent on tyrosine kinase pathway(s) and is partially dependent on mitogen-activated protein kinase/extracellular-signal regulated kinase 1/2. We believe that these pathways are biologically significant because VACV replication and dissemination was drastically affected when the infection was carried out in the presence of the relevant pharmacological inhibitors.
publishDate 2010
dc.date.none.fl_str_mv 2010-05-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762010000300005
url http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762010000300005
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0074-02762010000300005
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
dc.source.none.fl_str_mv Memórias do Instituto Oswaldo Cruz v.105 n.3 2010
reponame:Memórias do Instituto Oswaldo Cruz
instname:Fundação Oswaldo Cruz
instacron:FIOCRUZ
reponame_str Memórias do Instituto Oswaldo Cruz
collection Memórias do Instituto Oswaldo Cruz
instname_str Fundação Oswaldo Cruz
instacron_str FIOCRUZ
institution FIOCRUZ
repository.name.fl_str_mv Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruz
repository.mail.fl_str_mv
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