Identification of Transcribed Sequences (ESTs) in the Trypanosoma cruzi Genome Project
Autor(a) principal: | |
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Data de Publicação: | 1997 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Memórias do Instituto Oswaldo Cruz |
Texto Completo: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761997000600024 |
Resumo: | Random single pass sequencing of cDNA fragments, also known as generation of Expressed Sequence Tags (ESTs), has been highly successful in the study of the gene content of higher organisms, and forms an integral part of most genome projects, with the objective to identify new genes and targets for disease control and prevention and to generate mapping probes. In the Trypanosoma cruzi genome project, EST sequencing has also been a starting point, and here we report data on the first 797 sequences obtained, partly from a CL Brener epimastigote non-normalized library, partly on a normalized library. Only around 30% of the sequences obtained showed similarity with Genbank and dbEST databases, half of which with sequences already reported for T. cruzi. |
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Memórias do Instituto Oswaldo Cruz |
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Identification of Transcribed Sequences (ESTs) in the Trypanosoma cruzi Genome Projectexpressed sequence tags sequencingnormalized cDNA libraryTrypanosoma cruziclone CL BrenerParasite Genome ProjectsRandom single pass sequencing of cDNA fragments, also known as generation of Expressed Sequence Tags (ESTs), has been highly successful in the study of the gene content of higher organisms, and forms an integral part of most genome projects, with the objective to identify new genes and targets for disease control and prevention and to generate mapping probes. In the Trypanosoma cruzi genome project, EST sequencing has also been a starting point, and here we report data on the first 797 sequences obtained, partly from a CL Brener epimastigote non-normalized library, partly on a normalized library. Only around 30% of the sequences obtained showed similarity with Genbank and dbEST databases, half of which with sequences already reported for T. cruzi.Instituto Oswaldo Cruz, Ministério da Saúde1997-11-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761997000600024Memórias do Instituto Oswaldo Cruz v.92 n.6 1997reponame:Memórias do Instituto Oswaldo Cruzinstname:Fundação Oswaldo Cruzinstacron:FIOCRUZ10.1590/S0074-02761997000600024info:eu-repo/semantics/openAccessBrandão,AdeíltonUrmenyi,TuranRondinelli,EdsonGonzalez,AntonioMiranda,Antonio B deDegrave,Wimeng2020-04-25T17:47:46Zhttp://www.scielo.br/oai/scielo-oai.php0074-02761678-8060opendoar:null2020-04-26 02:07:45.056Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruztrue |
dc.title.none.fl_str_mv |
Identification of Transcribed Sequences (ESTs) in the Trypanosoma cruzi Genome Project |
title |
Identification of Transcribed Sequences (ESTs) in the Trypanosoma cruzi Genome Project |
spellingShingle |
Identification of Transcribed Sequences (ESTs) in the Trypanosoma cruzi Genome Project Brandão,Adeílton expressed sequence tags sequencing normalized cDNA library Trypanosoma cruzi clone CL Brener Parasite Genome Projects |
title_short |
Identification of Transcribed Sequences (ESTs) in the Trypanosoma cruzi Genome Project |
title_full |
Identification of Transcribed Sequences (ESTs) in the Trypanosoma cruzi Genome Project |
title_fullStr |
Identification of Transcribed Sequences (ESTs) in the Trypanosoma cruzi Genome Project |
title_full_unstemmed |
Identification of Transcribed Sequences (ESTs) in the Trypanosoma cruzi Genome Project |
title_sort |
Identification of Transcribed Sequences (ESTs) in the Trypanosoma cruzi Genome Project |
author |
Brandão,Adeílton |
author_facet |
Brandão,Adeílton Urmenyi,Turan Rondinelli,Edson Gonzalez,Antonio Miranda,Antonio B de Degrave,Wim |
author_role |
author |
author2 |
Urmenyi,Turan Rondinelli,Edson Gonzalez,Antonio Miranda,Antonio B de Degrave,Wim |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Brandão,Adeílton Urmenyi,Turan Rondinelli,Edson Gonzalez,Antonio Miranda,Antonio B de Degrave,Wim |
dc.subject.por.fl_str_mv |
expressed sequence tags sequencing normalized cDNA library Trypanosoma cruzi clone CL Brener Parasite Genome Projects |
topic |
expressed sequence tags sequencing normalized cDNA library Trypanosoma cruzi clone CL Brener Parasite Genome Projects |
dc.description.none.fl_txt_mv |
Random single pass sequencing of cDNA fragments, also known as generation of Expressed Sequence Tags (ESTs), has been highly successful in the study of the gene content of higher organisms, and forms an integral part of most genome projects, with the objective to identify new genes and targets for disease control and prevention and to generate mapping probes. In the Trypanosoma cruzi genome project, EST sequencing has also been a starting point, and here we report data on the first 797 sequences obtained, partly from a CL Brener epimastigote non-normalized library, partly on a normalized library. Only around 30% of the sequences obtained showed similarity with Genbank and dbEST databases, half of which with sequences already reported for T. cruzi. |
description |
Random single pass sequencing of cDNA fragments, also known as generation of Expressed Sequence Tags (ESTs), has been highly successful in the study of the gene content of higher organisms, and forms an integral part of most genome projects, with the objective to identify new genes and targets for disease control and prevention and to generate mapping probes. In the Trypanosoma cruzi genome project, EST sequencing has also been a starting point, and here we report data on the first 797 sequences obtained, partly from a CL Brener epimastigote non-normalized library, partly on a normalized library. Only around 30% of the sequences obtained showed similarity with Genbank and dbEST databases, half of which with sequences already reported for T. cruzi. |
publishDate |
1997 |
dc.date.none.fl_str_mv |
1997-11-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761997000600024 |
url |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761997000600024 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S0074-02761997000600024 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Instituto Oswaldo Cruz, Ministério da Saúde |
publisher.none.fl_str_mv |
Instituto Oswaldo Cruz, Ministério da Saúde |
dc.source.none.fl_str_mv |
Memórias do Instituto Oswaldo Cruz v.92 n.6 1997 reponame:Memórias do Instituto Oswaldo Cruz instname:Fundação Oswaldo Cruz instacron:FIOCRUZ |
reponame_str |
Memórias do Instituto Oswaldo Cruz |
collection |
Memórias do Instituto Oswaldo Cruz |
instname_str |
Fundação Oswaldo Cruz |
instacron_str |
FIOCRUZ |
institution |
FIOCRUZ |
repository.name.fl_str_mv |
Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruz |
repository.mail.fl_str_mv |
|
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1669937671579893760 |