Serological detection of West Nile virus in horses and chicken from Pantanal, Brazil

Detalhes bibliográficos
Autor(a) principal: Melandri,Vanessa
Data de Publicação: 2012
Outros Autores: Guimarães,Anthony Érico, Komar,Nicholas, Nogueira,Maurício L, Mondini,Adriano, Fernandez-Sesma,Ana, Alencar,Jeronimo, Bosch,Irene
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Memórias do Instituto Oswaldo Cruz
Texto Completo: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762012000800020
Resumo: In an effort to detect West Nile virus (WNV) in Brazil, we sampled serum from horses and chickens from the Pantanal region of the state of Mato Grosso and tested for flavivirus-reactive antibodies by blocking ELISA. The positive samples were further confirmed for serological evidence of WNV infection in three (8%) of the 38 horses and one (3.2%) of the 31 chickens using an 80% plaque-reduction neutralisation test (PRNT80). These results provide evidence of the circulation of WNV in chickens and horses in Pantanal.
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spelling Serological detection of West Nile virus in horses and chicken from Pantanal, BrazilWNVb-ELISAPRNTBrazilIn an effort to detect West Nile virus (WNV) in Brazil, we sampled serum from horses and chickens from the Pantanal region of the state of Mato Grosso and tested for flavivirus-reactive antibodies by blocking ELISA. The positive samples were further confirmed for serological evidence of WNV infection in three (8%) of the 38 horses and one (3.2%) of the 31 chickens using an 80% plaque-reduction neutralisation test (PRNT80). These results provide evidence of the circulation of WNV in chickens and horses in Pantanal.Instituto Oswaldo Cruz, Ministério da Saúde2012-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762012000800020Memórias do Instituto Oswaldo Cruz v.107 n.8 2012reponame:Memórias do Instituto Oswaldo Cruzinstname:Fundação Oswaldo Cruzinstacron:FIOCRUZ10.1590/S0074-02762012000800020info:eu-repo/semantics/openAccessMelandri,VanessaGuimarães,Anthony ÉricoKomar,NicholasNogueira,Maurício LMondini,AdrianoFernandez-Sesma,AnaAlencar,JeronimoBosch,Ireneeng2020-04-25T17:51:18Zhttp://www.scielo.br/oai/scielo-oai.php0074-02761678-8060opendoar:null2020-04-26 02:18:40.622Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruztrue
dc.title.none.fl_str_mv Serological detection of West Nile virus in horses and chicken from Pantanal, Brazil
title Serological detection of West Nile virus in horses and chicken from Pantanal, Brazil
spellingShingle Serological detection of West Nile virus in horses and chicken from Pantanal, Brazil
Melandri,Vanessa
WNV
b-ELISA
PRNT
Brazil
title_short Serological detection of West Nile virus in horses and chicken from Pantanal, Brazil
title_full Serological detection of West Nile virus in horses and chicken from Pantanal, Brazil
title_fullStr Serological detection of West Nile virus in horses and chicken from Pantanal, Brazil
title_full_unstemmed Serological detection of West Nile virus in horses and chicken from Pantanal, Brazil
title_sort Serological detection of West Nile virus in horses and chicken from Pantanal, Brazil
author Melandri,Vanessa
author_facet Melandri,Vanessa
Guimarães,Anthony Érico
Komar,Nicholas
Nogueira,Maurício L
Mondini,Adriano
Fernandez-Sesma,Ana
Alencar,Jeronimo
Bosch,Irene
author_role author
author2 Guimarães,Anthony Érico
Komar,Nicholas
Nogueira,Maurício L
Mondini,Adriano
Fernandez-Sesma,Ana
Alencar,Jeronimo
Bosch,Irene
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Melandri,Vanessa
Guimarães,Anthony Érico
Komar,Nicholas
Nogueira,Maurício L
Mondini,Adriano
Fernandez-Sesma,Ana
Alencar,Jeronimo
Bosch,Irene
dc.subject.por.fl_str_mv WNV
b-ELISA
PRNT
Brazil
topic WNV
b-ELISA
PRNT
Brazil
dc.description.none.fl_txt_mv In an effort to detect West Nile virus (WNV) in Brazil, we sampled serum from horses and chickens from the Pantanal region of the state of Mato Grosso and tested for flavivirus-reactive antibodies by blocking ELISA. The positive samples were further confirmed for serological evidence of WNV infection in three (8%) of the 38 horses and one (3.2%) of the 31 chickens using an 80% plaque-reduction neutralisation test (PRNT80). These results provide evidence of the circulation of WNV in chickens and horses in Pantanal.
description In an effort to detect West Nile virus (WNV) in Brazil, we sampled serum from horses and chickens from the Pantanal region of the state of Mato Grosso and tested for flavivirus-reactive antibodies by blocking ELISA. The positive samples were further confirmed for serological evidence of WNV infection in three (8%) of the 38 horses and one (3.2%) of the 31 chickens using an 80% plaque-reduction neutralisation test (PRNT80). These results provide evidence of the circulation of WNV in chickens and horses in Pantanal.
publishDate 2012
dc.date.none.fl_str_mv 2012-12-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762012000800020
url http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762012000800020
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0074-02762012000800020
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
dc.source.none.fl_str_mv Memórias do Instituto Oswaldo Cruz v.107 n.8 2012
reponame:Memórias do Instituto Oswaldo Cruz
instname:Fundação Oswaldo Cruz
instacron:FIOCRUZ
reponame_str Memórias do Instituto Oswaldo Cruz
collection Memórias do Instituto Oswaldo Cruz
instname_str Fundação Oswaldo Cruz
instacron_str FIOCRUZ
institution FIOCRUZ
repository.name.fl_str_mv Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruz
repository.mail.fl_str_mv
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