Serological detection of West Nile virus in horses and chicken from Pantanal, Brazil

Detalhes bibliográficos
Autor(a) principal: Melandri, Vanessa
Data de Publicação: 2012
Outros Autores: Guimarães, Anthony Érico, Komar, Nicholas, Nogueira, Maurício L, Mondini, Adriano [UNESP], Fernandez-Sesma, Ana, Alencar, Jeronimo, Bosch, Irene
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1590/S0074-02762012000800020
http://hdl.handle.net/11449/8025
Resumo: In an effort to detect West Nile virus (WNV) in Brazil, we sampled serum from horses and chickens from the Pantanal region of the state of Mato Grosso and tested for flavivirus-reactive antibodies by blocking ELISA. The positive samples were further confirmed for serological evidence of WNV infection in three (8%) of the 38 horses and one (3.2%) of the 31 chickens using an 80% plaque-reduction neutralisation test (PRNT80). These results provide evidence of the circulation of WNV in chickens and horses in Pantanal.
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spelling Serological detection of West Nile virus in horses and chicken from Pantanal, BrazilWNVb-ELISAPRNTBrazilIn an effort to detect West Nile virus (WNV) in Brazil, we sampled serum from horses and chickens from the Pantanal region of the state of Mato Grosso and tested for flavivirus-reactive antibodies by blocking ELISA. The positive samples were further confirmed for serological evidence of WNV infection in three (8%) of the 38 horses and one (3.2%) of the 31 chickens using an 80% plaque-reduction neutralisation test (PRNT80). These results provide evidence of the circulation of WNV in chickens and horses in Pantanal.Fiocruz Instituto Oswaldo Cruz Laboratório de DipteraCenters for Disease Control and PreventionFaculdade de Medicina de São José do Rio Preto (FAMERP)Universidade Estadual Paulista Faculdade de Ciências FarmacêuticasMount Sinai School of MedicineMassachusetts Institute of TechnologyUniversidade Estadual Paulista Faculdade de Ciências FarmacêuticasInstituto Oswaldo Cruz, Ministério da SaúdeFiocruz Instituto Oswaldo Cruz Laboratório de DipteraCenters for Disease Control and PreventionFaculdade de Medicina de São José do Rio Preto (FAMERP)Universidade Estadual Paulista (Unesp)Mount Sinai School of MedicineMassachusetts Institute of Technology (MIT)Melandri, VanessaGuimarães, Anthony ÉricoKomar, NicholasNogueira, Maurício LMondini, Adriano [UNESP]Fernandez-Sesma, AnaAlencar, JeronimoBosch, Irene2014-05-20T13:25:22Z2014-05-20T13:25:22Z2012-12-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1073-1075application/pdfhttp://dx.doi.org/10.1590/S0074-02762012000800020Memórias do Instituto Oswaldo Cruz. Instituto Oswaldo Cruz, Ministério da Saúde, v. 107, n. 8, p. 1073-1075, 2012.0074-0276http://hdl.handle.net/11449/802510.1590/S0074-02762012000800020S0074-02762012000800020S0074-02762012000800020.pdf58211426247203980000-0002-5557-9721SciELOreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengMemórias do Instituto Oswaldo Cruz2.8331,172info:eu-repo/semantics/openAccess2023-11-12T06:07:50Zoai:repositorio.unesp.br:11449/8025Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-11-12T06:07:50Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Serological detection of West Nile virus in horses and chicken from Pantanal, Brazil
title Serological detection of West Nile virus in horses and chicken from Pantanal, Brazil
spellingShingle Serological detection of West Nile virus in horses and chicken from Pantanal, Brazil
Melandri, Vanessa
WNV
b-ELISA
PRNT
Brazil
title_short Serological detection of West Nile virus in horses and chicken from Pantanal, Brazil
title_full Serological detection of West Nile virus in horses and chicken from Pantanal, Brazil
title_fullStr Serological detection of West Nile virus in horses and chicken from Pantanal, Brazil
title_full_unstemmed Serological detection of West Nile virus in horses and chicken from Pantanal, Brazil
title_sort Serological detection of West Nile virus in horses and chicken from Pantanal, Brazil
author Melandri, Vanessa
author_facet Melandri, Vanessa
Guimarães, Anthony Érico
Komar, Nicholas
Nogueira, Maurício L
Mondini, Adriano [UNESP]
Fernandez-Sesma, Ana
Alencar, Jeronimo
Bosch, Irene
author_role author
author2 Guimarães, Anthony Érico
Komar, Nicholas
Nogueira, Maurício L
Mondini, Adriano [UNESP]
Fernandez-Sesma, Ana
Alencar, Jeronimo
Bosch, Irene
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Fiocruz Instituto Oswaldo Cruz Laboratório de Diptera
Centers for Disease Control and Prevention
Faculdade de Medicina de São José do Rio Preto (FAMERP)
Universidade Estadual Paulista (Unesp)
Mount Sinai School of Medicine
Massachusetts Institute of Technology (MIT)
dc.contributor.author.fl_str_mv Melandri, Vanessa
Guimarães, Anthony Érico
Komar, Nicholas
Nogueira, Maurício L
Mondini, Adriano [UNESP]
Fernandez-Sesma, Ana
Alencar, Jeronimo
Bosch, Irene
dc.subject.por.fl_str_mv WNV
b-ELISA
PRNT
Brazil
topic WNV
b-ELISA
PRNT
Brazil
description In an effort to detect West Nile virus (WNV) in Brazil, we sampled serum from horses and chickens from the Pantanal region of the state of Mato Grosso and tested for flavivirus-reactive antibodies by blocking ELISA. The positive samples were further confirmed for serological evidence of WNV infection in three (8%) of the 38 horses and one (3.2%) of the 31 chickens using an 80% plaque-reduction neutralisation test (PRNT80). These results provide evidence of the circulation of WNV in chickens and horses in Pantanal.
publishDate 2012
dc.date.none.fl_str_mv 2012-12-01
2014-05-20T13:25:22Z
2014-05-20T13:25:22Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1590/S0074-02762012000800020
Memórias do Instituto Oswaldo Cruz. Instituto Oswaldo Cruz, Ministério da Saúde, v. 107, n. 8, p. 1073-1075, 2012.
0074-0276
http://hdl.handle.net/11449/8025
10.1590/S0074-02762012000800020
S0074-02762012000800020
S0074-02762012000800020.pdf
5821142624720398
0000-0002-5557-9721
url http://dx.doi.org/10.1590/S0074-02762012000800020
http://hdl.handle.net/11449/8025
identifier_str_mv Memórias do Instituto Oswaldo Cruz. Instituto Oswaldo Cruz, Ministério da Saúde, v. 107, n. 8, p. 1073-1075, 2012.
0074-0276
10.1590/S0074-02762012000800020
S0074-02762012000800020
S0074-02762012000800020.pdf
5821142624720398
0000-0002-5557-9721
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Memórias do Instituto Oswaldo Cruz
2.833
1,172
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 1073-1075
application/pdf
dc.publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
dc.source.none.fl_str_mv SciELO
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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