Production and diagnostic application of recombinant domain III of West Nile envelope protein in Brazil

Detalhes bibliográficos
Autor(a) principal: Chávez,Juliana Helena
Data de Publicação: 2013
Outros Autores: Reis,Vinicius Pinho dos, Silva,Jaqueline Raymondi, Laure,Helen Julie, Rosa,José Cesar, Fonseca,Benedito Antônio Lopes da, Figueiredo,Luiz Tadeu Moraes
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Revista da Sociedade Brasileira de Medicina Tropical
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822013000100097
Resumo: INTRODUCTION: West Nile virus (WNV) is a flavivirus with a natural cycle involving mosquitoes and birds. Over the last 11 years, WNV has spread throughout the Americas with the imminent risk of its introduction in Brazil. METHODS: Envelope protein domain III of WNV (rDIII) was bacterially expressed and purified. An enzyme-linked immunosorbent assay with WNV rDIII antigen was standardized against mouse immune fluids (MIAFs) of different flavivirus. RESULTS: WNV rDIII reacted strongly with St. Louis encephalitis virus (SLEV) MIAF but not with other flaviviruses. CONCLUSIONS: This antigen may be a potentially useful tool for serologic diagnosis and may contribute in future epidemiological surveillance of WNV infections in Brazil.
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spelling Production and diagnostic application of recombinant domain III of West Nile envelope protein in BrazilDiagnosisWNV antibodiesELISA INTRODUCTION: West Nile virus (WNV) is a flavivirus with a natural cycle involving mosquitoes and birds. Over the last 11 years, WNV has spread throughout the Americas with the imminent risk of its introduction in Brazil. METHODS: Envelope protein domain III of WNV (rDIII) was bacterially expressed and purified. An enzyme-linked immunosorbent assay with WNV rDIII antigen was standardized against mouse immune fluids (MIAFs) of different flavivirus. RESULTS: WNV rDIII reacted strongly with St. Louis encephalitis virus (SLEV) MIAF but not with other flaviviruses. CONCLUSIONS: This antigen may be a potentially useful tool for serologic diagnosis and may contribute in future epidemiological surveillance of WNV infections in Brazil.Sociedade Brasileira de Medicina Tropical - SBMT2013-02-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822013000100097Revista da Sociedade Brasileira de Medicina Tropical v.46 n.1 2013reponame:Revista da Sociedade Brasileira de Medicina Tropicalinstname:Sociedade Brasileira de Medicina Tropical (SBMT)instacron:SBMT10.1590/0037-868214142013info:eu-repo/semantics/openAccessChávez,Juliana HelenaReis,Vinicius Pinho dosSilva,Jaqueline RaymondiLaure,Helen JulieRosa,José CesarFonseca,Benedito Antônio Lopes daFigueiredo,Luiz Tadeu Moraeseng2021-03-18T00:00:00Zoai:scielo:S0037-86822013000100097Revistahttps://www.sbmt.org.br/portal/revista/ONGhttps://old.scielo.br/oai/scielo-oai.php||dalmo@rsbmt.uftm.edu.br|| rsbmt@rsbmt.uftm.edu.br1678-98490037-8682opendoar:2021-03-18T00:00Revista da Sociedade Brasileira de Medicina Tropical - Sociedade Brasileira de Medicina Tropical (SBMT)false
dc.title.none.fl_str_mv Production and diagnostic application of recombinant domain III of West Nile envelope protein in Brazil
title Production and diagnostic application of recombinant domain III of West Nile envelope protein in Brazil
spellingShingle Production and diagnostic application of recombinant domain III of West Nile envelope protein in Brazil
Chávez,Juliana Helena
Diagnosis
WNV antibodies
ELISA
title_short Production and diagnostic application of recombinant domain III of West Nile envelope protein in Brazil
title_full Production and diagnostic application of recombinant domain III of West Nile envelope protein in Brazil
title_fullStr Production and diagnostic application of recombinant domain III of West Nile envelope protein in Brazil
title_full_unstemmed Production and diagnostic application of recombinant domain III of West Nile envelope protein in Brazil
title_sort Production and diagnostic application of recombinant domain III of West Nile envelope protein in Brazil
author Chávez,Juliana Helena
author_facet Chávez,Juliana Helena
Reis,Vinicius Pinho dos
Silva,Jaqueline Raymondi
Laure,Helen Julie
Rosa,José Cesar
Fonseca,Benedito Antônio Lopes da
Figueiredo,Luiz Tadeu Moraes
author_role author
author2 Reis,Vinicius Pinho dos
Silva,Jaqueline Raymondi
Laure,Helen Julie
Rosa,José Cesar
Fonseca,Benedito Antônio Lopes da
Figueiredo,Luiz Tadeu Moraes
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Chávez,Juliana Helena
Reis,Vinicius Pinho dos
Silva,Jaqueline Raymondi
Laure,Helen Julie
Rosa,José Cesar
Fonseca,Benedito Antônio Lopes da
Figueiredo,Luiz Tadeu Moraes
dc.subject.por.fl_str_mv Diagnosis
WNV antibodies
ELISA
topic Diagnosis
WNV antibodies
ELISA
description INTRODUCTION: West Nile virus (WNV) is a flavivirus with a natural cycle involving mosquitoes and birds. Over the last 11 years, WNV has spread throughout the Americas with the imminent risk of its introduction in Brazil. METHODS: Envelope protein domain III of WNV (rDIII) was bacterially expressed and purified. An enzyme-linked immunosorbent assay with WNV rDIII antigen was standardized against mouse immune fluids (MIAFs) of different flavivirus. RESULTS: WNV rDIII reacted strongly with St. Louis encephalitis virus (SLEV) MIAF but not with other flaviviruses. CONCLUSIONS: This antigen may be a potentially useful tool for serologic diagnosis and may contribute in future epidemiological surveillance of WNV infections in Brazil.
publishDate 2013
dc.date.none.fl_str_mv 2013-02-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822013000100097
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822013000100097
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/0037-868214142013
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Medicina Tropical - SBMT
publisher.none.fl_str_mv Sociedade Brasileira de Medicina Tropical - SBMT
dc.source.none.fl_str_mv Revista da Sociedade Brasileira de Medicina Tropical v.46 n.1 2013
reponame:Revista da Sociedade Brasileira de Medicina Tropical
instname:Sociedade Brasileira de Medicina Tropical (SBMT)
instacron:SBMT
instname_str Sociedade Brasileira de Medicina Tropical (SBMT)
instacron_str SBMT
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reponame_str Revista da Sociedade Brasileira de Medicina Tropical
collection Revista da Sociedade Brasileira de Medicina Tropical
repository.name.fl_str_mv Revista da Sociedade Brasileira de Medicina Tropical - Sociedade Brasileira de Medicina Tropical (SBMT)
repository.mail.fl_str_mv ||dalmo@rsbmt.uftm.edu.br|| rsbmt@rsbmt.uftm.edu.br
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