Effect of serum sample inactivation on the performance of latex agglutination test for paracoccidioidomycosis serodiagnosis

Detalhes bibliográficos
Autor(a) principal: Silveira-Gomes,Fabíola
Data de Publicação: 2012
Outros Autores: Marques-da-Silva,Silvia Helena
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Memórias do Instituto Oswaldo Cruz
Texto Completo: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762012000400011
Resumo: Paracoccidioidomycosis is diagnosed from the direct observation of the causative agent, but serology can facilitate and decrease the time required for diagnosis. The objective of this study was to determine the influence of serum sample inactivation on the performance of the latex agglutination test (LAT) for detecting antibodies against Paracoccidioides brasiliensis. The sensitivity of LAT from inactivated or non-inactivated samples was 73% and 83%, respectively and the LAT selectivity was 79% and 90%, respectively. The LAT evaluated here was no more specific than the double-immunodiffusion assay. We suggest the investigation of other methods for improving the LAT, such as the use of deglycosylated antigen.
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spelling Effect of serum sample inactivation on the performance of latex agglutination test for paracoccidioidomycosis serodiagnosisparacoccidioidomycosisserodiagnosislatex agglutination testinactivation of samplesParacoccidioidomycosis is diagnosed from the direct observation of the causative agent, but serology can facilitate and decrease the time required for diagnosis. The objective of this study was to determine the influence of serum sample inactivation on the performance of the latex agglutination test (LAT) for detecting antibodies against Paracoccidioides brasiliensis. The sensitivity of LAT from inactivated or non-inactivated samples was 73% and 83%, respectively and the LAT selectivity was 79% and 90%, respectively. The LAT evaluated here was no more specific than the double-immunodiffusion assay. We suggest the investigation of other methods for improving the LAT, such as the use of deglycosylated antigen.Instituto Oswaldo Cruz, Ministério da Saúde2012-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762012000400011Memórias do Instituto Oswaldo Cruz v.107 n.4 2012reponame:Memórias do Instituto Oswaldo Cruzinstname:Fundação Oswaldo Cruzinstacron:FIOCRUZ10.1590/S0074-02762012000400011info:eu-repo/semantics/openAccessSilveira-Gomes,FabíolaMarques-da-Silva,Silvia Helenaeng2020-04-25T17:51:13Zhttp://www.scielo.br/oai/scielo-oai.php0074-02761678-8060opendoar:null2020-04-26 02:18:21.146Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruztrue
dc.title.none.fl_str_mv Effect of serum sample inactivation on the performance of latex agglutination test for paracoccidioidomycosis serodiagnosis
title Effect of serum sample inactivation on the performance of latex agglutination test for paracoccidioidomycosis serodiagnosis
spellingShingle Effect of serum sample inactivation on the performance of latex agglutination test for paracoccidioidomycosis serodiagnosis
Silveira-Gomes,Fabíola
paracoccidioidomycosis
serodiagnosis
latex agglutination test
inactivation of samples
title_short Effect of serum sample inactivation on the performance of latex agglutination test for paracoccidioidomycosis serodiagnosis
title_full Effect of serum sample inactivation on the performance of latex agglutination test for paracoccidioidomycosis serodiagnosis
title_fullStr Effect of serum sample inactivation on the performance of latex agglutination test for paracoccidioidomycosis serodiagnosis
title_full_unstemmed Effect of serum sample inactivation on the performance of latex agglutination test for paracoccidioidomycosis serodiagnosis
title_sort Effect of serum sample inactivation on the performance of latex agglutination test for paracoccidioidomycosis serodiagnosis
author Silveira-Gomes,Fabíola
author_facet Silveira-Gomes,Fabíola
Marques-da-Silva,Silvia Helena
author_role author
author2 Marques-da-Silva,Silvia Helena
author2_role author
dc.contributor.author.fl_str_mv Silveira-Gomes,Fabíola
Marques-da-Silva,Silvia Helena
dc.subject.por.fl_str_mv paracoccidioidomycosis
serodiagnosis
latex agglutination test
inactivation of samples
topic paracoccidioidomycosis
serodiagnosis
latex agglutination test
inactivation of samples
dc.description.none.fl_txt_mv Paracoccidioidomycosis is diagnosed from the direct observation of the causative agent, but serology can facilitate and decrease the time required for diagnosis. The objective of this study was to determine the influence of serum sample inactivation on the performance of the latex agglutination test (LAT) for detecting antibodies against Paracoccidioides brasiliensis. The sensitivity of LAT from inactivated or non-inactivated samples was 73% and 83%, respectively and the LAT selectivity was 79% and 90%, respectively. The LAT evaluated here was no more specific than the double-immunodiffusion assay. We suggest the investigation of other methods for improving the LAT, such as the use of deglycosylated antigen.
description Paracoccidioidomycosis is diagnosed from the direct observation of the causative agent, but serology can facilitate and decrease the time required for diagnosis. The objective of this study was to determine the influence of serum sample inactivation on the performance of the latex agglutination test (LAT) for detecting antibodies against Paracoccidioides brasiliensis. The sensitivity of LAT from inactivated or non-inactivated samples was 73% and 83%, respectively and the LAT selectivity was 79% and 90%, respectively. The LAT evaluated here was no more specific than the double-immunodiffusion assay. We suggest the investigation of other methods for improving the LAT, such as the use of deglycosylated antigen.
publishDate 2012
dc.date.none.fl_str_mv 2012-06-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762012000400011
url http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762012000400011
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0074-02762012000400011
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
dc.source.none.fl_str_mv Memórias do Instituto Oswaldo Cruz v.107 n.4 2012
reponame:Memórias do Instituto Oswaldo Cruz
instname:Fundação Oswaldo Cruz
instacron:FIOCRUZ
reponame_str Memórias do Instituto Oswaldo Cruz
collection Memórias do Instituto Oswaldo Cruz
instname_str Fundação Oswaldo Cruz
instacron_str FIOCRUZ
institution FIOCRUZ
repository.name.fl_str_mv Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruz
repository.mail.fl_str_mv
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