In vitro activity of naturally occurring peptides (defensins) against Listeria monocytogenes

Detalhes bibliográficos
Autor(a) principal: Nascimento, Maria da Graça F.
Data de Publicação: 1994
Outros Autores: Cullor, James S., Selsted, Michael E.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Cadernos de Saúde Pública
Texto Completo: https://cadernos.ensp.fiocruz.br/ojs/index.php/csp/article/view/621
Resumo: Autoclaved distilled water samples were inoculated with L. monocytogenes strain V7 and strain VPH-1, and incubated aerobically, at 30 C for 48 hours. Each strain was tested individually, and growth curves were determined at 1, 2, 3, 4, 5, 21, 24, and 48 hours. The growth or survival of L. monocytogenes was similar for both strains, with survivors at 24 hour-incubation. The microbicidal activity of one synthetic cationic peptide (NP-2) was examined against L. monocytogenes strain V7, in a water system. Antibacterial activity of NP-2 (1, 5, and 10 g/ml) was best expressed at 60 minute-incubation, with 10 g/ml of peptide, at 30 C.
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spelling In vitro activity of naturally occurring peptides (defensins) against Listeria monocytogenesListeria monocytogenesDefensinsControlLytic PeptideAutoclaved distilled water samples were inoculated with L. monocytogenes strain V7 and strain VPH-1, and incubated aerobically, at 30 C for 48 hours. Each strain was tested individually, and growth curves were determined at 1, 2, 3, 4, 5, 21, 24, and 48 hours. The growth or survival of L. monocytogenes was similar for both strains, with survivors at 24 hour-incubation. The microbicidal activity of one synthetic cationic peptide (NP-2) was examined against L. monocytogenes strain V7, in a water system. Antibacterial activity of NP-2 (1, 5, and 10 g/ml) was best expressed at 60 minute-incubation, with 10 g/ml of peptide, at 30 C.Amostras de água destilada, autoclavadas, foram inoculadas com L. monocytogenes cepa V7 e cepa VPH-1, e incubadas, aerobicamente, a 30ºC por 48 horas. Cada cepa foi testada individualmente, e determinou-se curvas de crescimento a 1, 2, 3, 4, 5, 21, 24, e 48 horas. O crescimento ou sobrevivência das duas cepas foi semelhante e encontrou-se sobreviventes em 24 horas de incubação. Examinou-se a atividade bactericida de um dos peptídeos catiônicos sintéticos (NP-2) contra L. monocytogenes cepa V7, em sistema aquoso. A atividade antibacteriana de NP-2 (1, 5, and 10µg/ml) foi melhor aos 60 minutos de incubação, com 10µg/ml de peptídeo, a 30 C.Reports in Public HealthCadernos de Saúde Pública1994-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlapplication/pdfhttps://cadernos.ensp.fiocruz.br/ojs/index.php/csp/article/view/621Reports in Public Health; Vol. 10 No. 4 (1994): October/DecemberCadernos de Saúde Pública; v. 10 n. 4 (1994): Outubro/Dezembro1678-44640102-311Xreponame:Cadernos de Saúde Públicainstname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZenghttps://cadernos.ensp.fiocruz.br/ojs/index.php/csp/article/view/621/1261https://cadernos.ensp.fiocruz.br/ojs/index.php/csp/article/view/621/1262Nascimento, Maria da Graça F.Cullor, James S.Selsted, Michael E.info:eu-repo/semantics/openAccess2024-03-06T15:25:59Zoai:ojs.teste-cadernos.ensp.fiocruz.br:article/621Revistahttps://cadernos.ensp.fiocruz.br/ojs/index.php/csphttps://cadernos.ensp.fiocruz.br/ojs/index.php/csp/oaicadernos@ensp.fiocruz.br||cadernos@ensp.fiocruz.br1678-44640102-311Xopendoar:2024-03-06T13:00:48.180625Cadernos de Saúde Pública - Fundação Oswaldo Cruz (FIOCRUZ)true
dc.title.none.fl_str_mv In vitro activity of naturally occurring peptides (defensins) against Listeria monocytogenes
title In vitro activity of naturally occurring peptides (defensins) against Listeria monocytogenes
spellingShingle In vitro activity of naturally occurring peptides (defensins) against Listeria monocytogenes
Nascimento, Maria da Graça F.
Listeria monocytogenes
Defensins
Control
Lytic Peptide
title_short In vitro activity of naturally occurring peptides (defensins) against Listeria monocytogenes
title_full In vitro activity of naturally occurring peptides (defensins) against Listeria monocytogenes
title_fullStr In vitro activity of naturally occurring peptides (defensins) against Listeria monocytogenes
title_full_unstemmed In vitro activity of naturally occurring peptides (defensins) against Listeria monocytogenes
title_sort In vitro activity of naturally occurring peptides (defensins) against Listeria monocytogenes
author Nascimento, Maria da Graça F.
author_facet Nascimento, Maria da Graça F.
Cullor, James S.
Selsted, Michael E.
author_role author
author2 Cullor, James S.
Selsted, Michael E.
author2_role author
author
dc.contributor.author.fl_str_mv Nascimento, Maria da Graça F.
Cullor, James S.
Selsted, Michael E.
dc.subject.por.fl_str_mv Listeria monocytogenes
Defensins
Control
Lytic Peptide
topic Listeria monocytogenes
Defensins
Control
Lytic Peptide
description Autoclaved distilled water samples were inoculated with L. monocytogenes strain V7 and strain VPH-1, and incubated aerobically, at 30 C for 48 hours. Each strain was tested individually, and growth curves were determined at 1, 2, 3, 4, 5, 21, 24, and 48 hours. The growth or survival of L. monocytogenes was similar for both strains, with survivors at 24 hour-incubation. The microbicidal activity of one synthetic cationic peptide (NP-2) was examined against L. monocytogenes strain V7, in a water system. Antibacterial activity of NP-2 (1, 5, and 10 g/ml) was best expressed at 60 minute-incubation, with 10 g/ml of peptide, at 30 C.
publishDate 1994
dc.date.none.fl_str_mv 1994-12-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://cadernos.ensp.fiocruz.br/ojs/index.php/csp/article/view/621
url https://cadernos.ensp.fiocruz.br/ojs/index.php/csp/article/view/621
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://cadernos.ensp.fiocruz.br/ojs/index.php/csp/article/view/621/1261
https://cadernos.ensp.fiocruz.br/ojs/index.php/csp/article/view/621/1262
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
application/pdf
dc.publisher.none.fl_str_mv Reports in Public Health
Cadernos de Saúde Pública
publisher.none.fl_str_mv Reports in Public Health
Cadernos de Saúde Pública
dc.source.none.fl_str_mv Reports in Public Health; Vol. 10 No. 4 (1994): October/December
Cadernos de Saúde Pública; v. 10 n. 4 (1994): Outubro/Dezembro
1678-4464
0102-311X
reponame:Cadernos de Saúde Pública
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Cadernos de Saúde Pública
collection Cadernos de Saúde Pública
repository.name.fl_str_mv Cadernos de Saúde Pública - Fundação Oswaldo Cruz (FIOCRUZ)
repository.mail.fl_str_mv cadernos@ensp.fiocruz.br||cadernos@ensp.fiocruz.br
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