Microbiological quality evaluation of dehydrated infant formulas after reconstitution and during storage in the lactary of a University Hospital

Detalhes bibliográficos
Autor(a) principal: Giangiarulo, Thamires Chrispim de Souza Carvalho
Data de Publicação: 2020
Outros Autores: Lima, Maria Thereza R. P. Dias de, Martins, Alfredo da Silva, Queiroz, Mara Lucia Penna, Miyahira, Roberta Fontanive
Tipo de documento: Artigo
Idioma: por
eng
Título da fonte: Vigilância Sanitária em Debate
Texto Completo: https://visaemdebate.incqs.fiocruz.br/index.php/visaemdebate/article/view/1393
Resumo: Introduction: Infant formulas (IF) fail to reproduce the immunological and digestibility properties of breast milk; however, they can meet the estimated nutritional needs when breastfeeding is prevented. Being rich in nutrients, artificial milk is an environment conducive to the growth of microorganisms. Objective: To perform microbiological analysis of IF intended for infants prepared in the lactary of a University Hospital of Rio de Janeiro, after reconstitution and storage. Method: 60 samples of IF reconstituted lactary were collected, immediately after preparation (time 0 h) and after 18 h storage under refrigeration (< 5°C), in two different shifts A and B. The Most Probable Number (MPN) for coliform growth at 35°C and 45°C and Plate Count of Bacillus cereus were calculated, and coagulase positive Staphylococcus (SCP) and qualitative analysis of the presence of Salmonella spp. were performed. Results: IF showed values of coliforms with growth at 35°C < 0.3 MPN /mL and B. cereus and SCP < 1 Colony Forming Unit (CFU)/mL, in addition to absence of Salmonella spp./25 mL at both times studied. Conclusions: There was no growth of the analyzed microorganisms in 100% of the samples, probably due to the adequate training of the food handlers of the studied hospital.
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spelling Microbiological quality evaluation of dehydrated infant formulas after reconstitution and during storage in the lactary of a University HospitalAvaliação da qualidade microbiológica de fórmulas infantis desidratadas após reconstituição e durante o armazenamento no lactário de um Hospital UniversitárioFórmulas InfantisLactárioAnálise MicrobiológicaControle de QualidadeInfant FormulasLactaryMicrobiological AnalysisQuality ControlIntroduction: Infant formulas (IF) fail to reproduce the immunological and digestibility properties of breast milk; however, they can meet the estimated nutritional needs when breastfeeding is prevented. Being rich in nutrients, artificial milk is an environment conducive to the growth of microorganisms. Objective: To perform microbiological analysis of IF intended for infants prepared in the lactary of a University Hospital of Rio de Janeiro, after reconstitution and storage. Method: 60 samples of IF reconstituted lactary were collected, immediately after preparation (time 0 h) and after 18 h storage under refrigeration (< 5°C), in two different shifts A and B. The Most Probable Number (MPN) for coliform growth at 35°C and 45°C and Plate Count of Bacillus cereus were calculated, and coagulase positive Staphylococcus (SCP) and qualitative analysis of the presence of Salmonella spp. were performed. Results: IF showed values of coliforms with growth at 35°C < 0.3 MPN /mL and B. cereus and SCP < 1 Colony Forming Unit (CFU)/mL, in addition to absence of Salmonella spp./25 mL at both times studied. Conclusions: There was no growth of the analyzed microorganisms in 100% of the samples, probably due to the adequate training of the food handlers of the studied hospital.Introdução: As fórmulas infantis (FI) não conseguem reproduzir as propriedades imunológicas e de digestibilidade do leite materno, entretanto conseguem atender às necessidades nutricionais estimadas quando há impedimento do aleitamento materno. Por ser rico em nutrientes, o leite artificial é um ambiente propício para o crescimento de microrganismos. Objetivo: Realizar análise microbiológica de FI destinadas a bebês preparadas no lactário de um Hospital Universitário do Rio de Janeiro, após reconstituição e armazenamento. Método: Foram coletadas 60 amostras de FI reconstituídas em lactário, logo após o preparo (tempo 0 h) e depois de 18 h de armazenamento sob refrigeração (< 5ºC), em dois plantões diferentes A e B. Foi realizada a técnica do Número mais Provável (NMP) para o crescimento de coliformes a 35ºC e a 45ºC, contagem em placa de Bacillus cereus e Staphylococcus coagulase positiva (SCP) e análise qualitativa de presença de Salmonella spp. Resultados: As FI apresentaram valores de coliformes com crescimento a 35ºC < 0,3 NMP/mL e de B. cereus e SCP < 1 Unidade Formadora de Colônia (UFC)/mL, além de ausência de Salmonella spp./25 mL, nos dois momentos estudados. Conclusões: Não houve crescimento dos microrganismos analisados em 100% das amostras, provavelmente devido ao treinamento adequado dos manipuladores de alimentos do lactário do hospital estudado.Instituto Nacional de Controle de Qualidade em Saúde2020-02-27info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion"Peer-reviewed article""Artículo revisado por pares""Artigo avaliado pelos pares"application/pdfapplication/pdfhttps://visaemdebate.incqs.fiocruz.br/index.php/visaemdebate/article/view/139310.22239/2317-269X.01393Health Surveillance under Debate: Society, Science & Technology ; Vol. 8 No. 1 (2020): February; 86-90Vigilancia en Salud en Debate: Sociedad, Ciencia y Tecnología; Vol. 8 Núm. 1 (2020): Febrero; 86-90Vigil Sanit Debate, Rio de Janeiro; v. 8 n. 1 (2020): Fevereiro; 86-902317-269Xreponame:Vigilância Sanitária em Debateinstname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZporenghttps://visaemdebate.incqs.fiocruz.br/index.php/visaemdebate/article/view/1393/1132https://visaemdebate.incqs.fiocruz.br/index.php/visaemdebate/article/view/1393/1190Copyright (c) 2020 Vigilância Sanitária em Debate: Sociedade, Ciência & Tecnologia (Health Surveillance under Debate: Society, Science & Technology) – Visa em Debatehttps://creativecommons.org/licenses/by-nc-nd/4.0info:eu-repo/semantics/openAccessGiangiarulo, Thamires Chrispim de Souza CarvalhoLima, Maria Thereza R. P. Dias deMartins, Alfredo da SilvaQueiroz, Mara Lucia PennaMiyahira, Roberta Fontanive2023-06-27T15:12:10Zoai:ojs.visaemdebate.incqs.fiocruz.br:article/1393Revistahttps://visaemdebate.incqs.fiocruz.br/index.php/visaemdebatePUBhttps://visaemdebate.incqs.fiocruz.br/index.php/visaemdebate/oaiincqs.visaemdebate@fiocruz.br || gisele.neves@fiocruz.br2317-269X2317-269Xopendoar:2023-06-27T15:12:10Vigilância Sanitária em Debate - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.none.fl_str_mv Microbiological quality evaluation of dehydrated infant formulas after reconstitution and during storage in the lactary of a University Hospital
Avaliação da qualidade microbiológica de fórmulas infantis desidratadas após reconstituição e durante o armazenamento no lactário de um Hospital Universitário
title Microbiological quality evaluation of dehydrated infant formulas after reconstitution and during storage in the lactary of a University Hospital
spellingShingle Microbiological quality evaluation of dehydrated infant formulas after reconstitution and during storage in the lactary of a University Hospital
Giangiarulo, Thamires Chrispim de Souza Carvalho
Fórmulas Infantis
Lactário
Análise Microbiológica
Controle de Qualidade
Infant Formulas
Lactary
Microbiological Analysis
Quality Control
title_short Microbiological quality evaluation of dehydrated infant formulas after reconstitution and during storage in the lactary of a University Hospital
title_full Microbiological quality evaluation of dehydrated infant formulas after reconstitution and during storage in the lactary of a University Hospital
title_fullStr Microbiological quality evaluation of dehydrated infant formulas after reconstitution and during storage in the lactary of a University Hospital
title_full_unstemmed Microbiological quality evaluation of dehydrated infant formulas after reconstitution and during storage in the lactary of a University Hospital
title_sort Microbiological quality evaluation of dehydrated infant formulas after reconstitution and during storage in the lactary of a University Hospital
author Giangiarulo, Thamires Chrispim de Souza Carvalho
author_facet Giangiarulo, Thamires Chrispim de Souza Carvalho
Lima, Maria Thereza R. P. Dias de
Martins, Alfredo da Silva
Queiroz, Mara Lucia Penna
Miyahira, Roberta Fontanive
author_role author
author2 Lima, Maria Thereza R. P. Dias de
Martins, Alfredo da Silva
Queiroz, Mara Lucia Penna
Miyahira, Roberta Fontanive
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Giangiarulo, Thamires Chrispim de Souza Carvalho
Lima, Maria Thereza R. P. Dias de
Martins, Alfredo da Silva
Queiroz, Mara Lucia Penna
Miyahira, Roberta Fontanive
dc.subject.por.fl_str_mv Fórmulas Infantis
Lactário
Análise Microbiológica
Controle de Qualidade
Infant Formulas
Lactary
Microbiological Analysis
Quality Control
topic Fórmulas Infantis
Lactário
Análise Microbiológica
Controle de Qualidade
Infant Formulas
Lactary
Microbiological Analysis
Quality Control
description Introduction: Infant formulas (IF) fail to reproduce the immunological and digestibility properties of breast milk; however, they can meet the estimated nutritional needs when breastfeeding is prevented. Being rich in nutrients, artificial milk is an environment conducive to the growth of microorganisms. Objective: To perform microbiological analysis of IF intended for infants prepared in the lactary of a University Hospital of Rio de Janeiro, after reconstitution and storage. Method: 60 samples of IF reconstituted lactary were collected, immediately after preparation (time 0 h) and after 18 h storage under refrigeration (< 5°C), in two different shifts A and B. The Most Probable Number (MPN) for coliform growth at 35°C and 45°C and Plate Count of Bacillus cereus were calculated, and coagulase positive Staphylococcus (SCP) and qualitative analysis of the presence of Salmonella spp. were performed. Results: IF showed values of coliforms with growth at 35°C < 0.3 MPN /mL and B. cereus and SCP < 1 Colony Forming Unit (CFU)/mL, in addition to absence of Salmonella spp./25 mL at both times studied. Conclusions: There was no growth of the analyzed microorganisms in 100% of the samples, probably due to the adequate training of the food handlers of the studied hospital.
publishDate 2020
dc.date.none.fl_str_mv 2020-02-27
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
"Peer-reviewed article"
"Artículo revisado por pares"
"Artigo avaliado pelos pares"
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://visaemdebate.incqs.fiocruz.br/index.php/visaemdebate/article/view/1393
10.22239/2317-269X.01393
url https://visaemdebate.incqs.fiocruz.br/index.php/visaemdebate/article/view/1393
identifier_str_mv 10.22239/2317-269X.01393
dc.language.iso.fl_str_mv por
eng
language por
eng
dc.relation.none.fl_str_mv https://visaemdebate.incqs.fiocruz.br/index.php/visaemdebate/article/view/1393/1132
https://visaemdebate.incqs.fiocruz.br/index.php/visaemdebate/article/view/1393/1190
dc.rights.driver.fl_str_mv https://creativecommons.org/licenses/by-nc-nd/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Instituto Nacional de Controle de Qualidade em Saúde
publisher.none.fl_str_mv Instituto Nacional de Controle de Qualidade em Saúde
dc.source.none.fl_str_mv Health Surveillance under Debate: Society, Science & Technology ; Vol. 8 No. 1 (2020): February; 86-90
Vigilancia en Salud en Debate: Sociedad, Ciencia y Tecnología; Vol. 8 Núm. 1 (2020): Febrero; 86-90
Vigil Sanit Debate, Rio de Janeiro; v. 8 n. 1 (2020): Fevereiro; 86-90
2317-269X
reponame:Vigilância Sanitária em Debate
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Vigilância Sanitária em Debate
collection Vigilância Sanitária em Debate
repository.name.fl_str_mv Vigilância Sanitária em Debate - Fundação Oswaldo Cruz (FIOCRUZ)
repository.mail.fl_str_mv incqs.visaemdebate@fiocruz.br || gisele.neves@fiocruz.br
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