Microbiological quality evaluation of dehydrated infant formulas after reconstitution and during storage in the lactary of a University Hospital
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | por eng |
Título da fonte: | Vigilância Sanitária em Debate |
Texto Completo: | https://visaemdebate.incqs.fiocruz.br/index.php/visaemdebate/article/view/1393 |
Resumo: | Introduction: Infant formulas (IF) fail to reproduce the immunological and digestibility properties of breast milk; however, they can meet the estimated nutritional needs when breastfeeding is prevented. Being rich in nutrients, artificial milk is an environment conducive to the growth of microorganisms. Objective: To perform microbiological analysis of IF intended for infants prepared in the lactary of a University Hospital of Rio de Janeiro, after reconstitution and storage. Method: 60 samples of IF reconstituted lactary were collected, immediately after preparation (time 0 h) and after 18 h storage under refrigeration (< 5°C), in two different shifts A and B. The Most Probable Number (MPN) for coliform growth at 35°C and 45°C and Plate Count of Bacillus cereus were calculated, and coagulase positive Staphylococcus (SCP) and qualitative analysis of the presence of Salmonella spp. were performed. Results: IF showed values of coliforms with growth at 35°C < 0.3 MPN /mL and B. cereus and SCP < 1 Colony Forming Unit (CFU)/mL, in addition to absence of Salmonella spp./25 mL at both times studied. Conclusions: There was no growth of the analyzed microorganisms in 100% of the samples, probably due to the adequate training of the food handlers of the studied hospital. |
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Microbiological quality evaluation of dehydrated infant formulas after reconstitution and during storage in the lactary of a University HospitalAvaliação da qualidade microbiológica de fórmulas infantis desidratadas após reconstituição e durante o armazenamento no lactário de um Hospital UniversitárioFórmulas InfantisLactárioAnálise MicrobiológicaControle de QualidadeInfant FormulasLactaryMicrobiological AnalysisQuality ControlIntroduction: Infant formulas (IF) fail to reproduce the immunological and digestibility properties of breast milk; however, they can meet the estimated nutritional needs when breastfeeding is prevented. Being rich in nutrients, artificial milk is an environment conducive to the growth of microorganisms. Objective: To perform microbiological analysis of IF intended for infants prepared in the lactary of a University Hospital of Rio de Janeiro, after reconstitution and storage. Method: 60 samples of IF reconstituted lactary were collected, immediately after preparation (time 0 h) and after 18 h storage under refrigeration (< 5°C), in two different shifts A and B. The Most Probable Number (MPN) for coliform growth at 35°C and 45°C and Plate Count of Bacillus cereus were calculated, and coagulase positive Staphylococcus (SCP) and qualitative analysis of the presence of Salmonella spp. were performed. Results: IF showed values of coliforms with growth at 35°C < 0.3 MPN /mL and B. cereus and SCP < 1 Colony Forming Unit (CFU)/mL, in addition to absence of Salmonella spp./25 mL at both times studied. Conclusions: There was no growth of the analyzed microorganisms in 100% of the samples, probably due to the adequate training of the food handlers of the studied hospital.Introdução: As fórmulas infantis (FI) não conseguem reproduzir as propriedades imunológicas e de digestibilidade do leite materno, entretanto conseguem atender às necessidades nutricionais estimadas quando há impedimento do aleitamento materno. Por ser rico em nutrientes, o leite artificial é um ambiente propício para o crescimento de microrganismos. Objetivo: Realizar análise microbiológica de FI destinadas a bebês preparadas no lactário de um Hospital Universitário do Rio de Janeiro, após reconstituição e armazenamento. Método: Foram coletadas 60 amostras de FI reconstituídas em lactário, logo após o preparo (tempo 0 h) e depois de 18 h de armazenamento sob refrigeração (< 5ºC), em dois plantões diferentes A e B. Foi realizada a técnica do Número mais Provável (NMP) para o crescimento de coliformes a 35ºC e a 45ºC, contagem em placa de Bacillus cereus e Staphylococcus coagulase positiva (SCP) e análise qualitativa de presença de Salmonella spp. Resultados: As FI apresentaram valores de coliformes com crescimento a 35ºC < 0,3 NMP/mL e de B. cereus e SCP < 1 Unidade Formadora de Colônia (UFC)/mL, além de ausência de Salmonella spp./25 mL, nos dois momentos estudados. Conclusões: Não houve crescimento dos microrganismos analisados em 100% das amostras, provavelmente devido ao treinamento adequado dos manipuladores de alimentos do lactário do hospital estudado.Instituto Nacional de Controle de Qualidade em Saúde2020-02-27info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion"Peer-reviewed article""Artículo revisado por pares""Artigo avaliado pelos pares"application/pdfapplication/pdfhttps://visaemdebate.incqs.fiocruz.br/index.php/visaemdebate/article/view/139310.22239/2317-269X.01393Health Surveillance under Debate: Society, Science & Technology ; Vol. 8 No. 1 (2020): February; 86-90Vigilancia en Salud en Debate: Sociedad, Ciencia y Tecnología; Vol. 8 Núm. 1 (2020): Febrero; 86-90Vigil Sanit Debate, Rio de Janeiro; v. 8 n. 1 (2020): Fevereiro; 86-902317-269Xreponame:Vigilância Sanitária em Debateinstname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZporenghttps://visaemdebate.incqs.fiocruz.br/index.php/visaemdebate/article/view/1393/1132https://visaemdebate.incqs.fiocruz.br/index.php/visaemdebate/article/view/1393/1190Copyright (c) 2020 Vigilância Sanitária em Debate: Sociedade, Ciência & Tecnologia (Health Surveillance under Debate: Society, Science & Technology) – Visa em Debatehttps://creativecommons.org/licenses/by-nc-nd/4.0info:eu-repo/semantics/openAccessGiangiarulo, Thamires Chrispim de Souza CarvalhoLima, Maria Thereza R. P. Dias deMartins, Alfredo da SilvaQueiroz, Mara Lucia PennaMiyahira, Roberta Fontanive2023-06-27T15:12:10Zoai:ojs.visaemdebate.incqs.fiocruz.br:article/1393Revistahttps://visaemdebate.incqs.fiocruz.br/index.php/visaemdebatePUBhttps://visaemdebate.incqs.fiocruz.br/index.php/visaemdebate/oaiincqs.visaemdebate@fiocruz.br || gisele.neves@fiocruz.br2317-269X2317-269Xopendoar:2023-06-27T15:12:10Vigilância Sanitária em Debate - Fundação Oswaldo Cruz (FIOCRUZ)false |
dc.title.none.fl_str_mv |
Microbiological quality evaluation of dehydrated infant formulas after reconstitution and during storage in the lactary of a University Hospital Avaliação da qualidade microbiológica de fórmulas infantis desidratadas após reconstituição e durante o armazenamento no lactário de um Hospital Universitário |
title |
Microbiological quality evaluation of dehydrated infant formulas after reconstitution and during storage in the lactary of a University Hospital |
spellingShingle |
Microbiological quality evaluation of dehydrated infant formulas after reconstitution and during storage in the lactary of a University Hospital Giangiarulo, Thamires Chrispim de Souza Carvalho Fórmulas Infantis Lactário Análise Microbiológica Controle de Qualidade Infant Formulas Lactary Microbiological Analysis Quality Control |
title_short |
Microbiological quality evaluation of dehydrated infant formulas after reconstitution and during storage in the lactary of a University Hospital |
title_full |
Microbiological quality evaluation of dehydrated infant formulas after reconstitution and during storage in the lactary of a University Hospital |
title_fullStr |
Microbiological quality evaluation of dehydrated infant formulas after reconstitution and during storage in the lactary of a University Hospital |
title_full_unstemmed |
Microbiological quality evaluation of dehydrated infant formulas after reconstitution and during storage in the lactary of a University Hospital |
title_sort |
Microbiological quality evaluation of dehydrated infant formulas after reconstitution and during storage in the lactary of a University Hospital |
author |
Giangiarulo, Thamires Chrispim de Souza Carvalho |
author_facet |
Giangiarulo, Thamires Chrispim de Souza Carvalho Lima, Maria Thereza R. P. Dias de Martins, Alfredo da Silva Queiroz, Mara Lucia Penna Miyahira, Roberta Fontanive |
author_role |
author |
author2 |
Lima, Maria Thereza R. P. Dias de Martins, Alfredo da Silva Queiroz, Mara Lucia Penna Miyahira, Roberta Fontanive |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Giangiarulo, Thamires Chrispim de Souza Carvalho Lima, Maria Thereza R. P. Dias de Martins, Alfredo da Silva Queiroz, Mara Lucia Penna Miyahira, Roberta Fontanive |
dc.subject.por.fl_str_mv |
Fórmulas Infantis Lactário Análise Microbiológica Controle de Qualidade Infant Formulas Lactary Microbiological Analysis Quality Control |
topic |
Fórmulas Infantis Lactário Análise Microbiológica Controle de Qualidade Infant Formulas Lactary Microbiological Analysis Quality Control |
description |
Introduction: Infant formulas (IF) fail to reproduce the immunological and digestibility properties of breast milk; however, they can meet the estimated nutritional needs when breastfeeding is prevented. Being rich in nutrients, artificial milk is an environment conducive to the growth of microorganisms. Objective: To perform microbiological analysis of IF intended for infants prepared in the lactary of a University Hospital of Rio de Janeiro, after reconstitution and storage. Method: 60 samples of IF reconstituted lactary were collected, immediately after preparation (time 0 h) and after 18 h storage under refrigeration (< 5°C), in two different shifts A and B. The Most Probable Number (MPN) for coliform growth at 35°C and 45°C and Plate Count of Bacillus cereus were calculated, and coagulase positive Staphylococcus (SCP) and qualitative analysis of the presence of Salmonella spp. were performed. Results: IF showed values of coliforms with growth at 35°C < 0.3 MPN /mL and B. cereus and SCP < 1 Colony Forming Unit (CFU)/mL, in addition to absence of Salmonella spp./25 mL at both times studied. Conclusions: There was no growth of the analyzed microorganisms in 100% of the samples, probably due to the adequate training of the food handlers of the studied hospital. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-02-27 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion "Peer-reviewed article" "Artículo revisado por pares" "Artigo avaliado pelos pares" |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://visaemdebate.incqs.fiocruz.br/index.php/visaemdebate/article/view/1393 10.22239/2317-269X.01393 |
url |
https://visaemdebate.incqs.fiocruz.br/index.php/visaemdebate/article/view/1393 |
identifier_str_mv |
10.22239/2317-269X.01393 |
dc.language.iso.fl_str_mv |
por eng |
language |
por eng |
dc.relation.none.fl_str_mv |
https://visaemdebate.incqs.fiocruz.br/index.php/visaemdebate/article/view/1393/1132 https://visaemdebate.incqs.fiocruz.br/index.php/visaemdebate/article/view/1393/1190 |
dc.rights.driver.fl_str_mv |
https://creativecommons.org/licenses/by-nc-nd/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-nd/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Instituto Nacional de Controle de Qualidade em Saúde |
publisher.none.fl_str_mv |
Instituto Nacional de Controle de Qualidade em Saúde |
dc.source.none.fl_str_mv |
Health Surveillance under Debate: Society, Science & Technology ; Vol. 8 No. 1 (2020): February; 86-90 Vigilancia en Salud en Debate: Sociedad, Ciencia y Tecnología; Vol. 8 Núm. 1 (2020): Febrero; 86-90 Vigil Sanit Debate, Rio de Janeiro; v. 8 n. 1 (2020): Fevereiro; 86-90 2317-269X reponame:Vigilância Sanitária em Debate instname:Fundação Oswaldo Cruz (FIOCRUZ) instacron:FIOCRUZ |
instname_str |
Fundação Oswaldo Cruz (FIOCRUZ) |
instacron_str |
FIOCRUZ |
institution |
FIOCRUZ |
reponame_str |
Vigilância Sanitária em Debate |
collection |
Vigilância Sanitária em Debate |
repository.name.fl_str_mv |
Vigilância Sanitária em Debate - Fundação Oswaldo Cruz (FIOCRUZ) |
repository.mail.fl_str_mv |
incqs.visaemdebate@fiocruz.br || gisele.neves@fiocruz.br |
_version_ |
1797042045787832320 |