Effect of Milk Renewal on Cell Viability In Vitro at Different Time Frames
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Dental Journal |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-64402017000400435 |
Resumo: | Abstract The purpose of this study was to evaluate if the renewal of milk as a storage medium, every 12, 24 and 48 h, is able to increase its ability to maintain human periodontal ligament fibroblasts (PDLF) viability over time. PDLF were soaked in Minimum Essential Medium at 37 °C (MEM-37) (positive control), tap water (Water) (negative control) and in skimmed milk (44 wells) at 5 °C and 20 °C. The skimmed milk was renewed every 12 h (Milk-12), 24 h (Milk-24) and 48 h (Milk-48) in 11 wells of each plate, and the milk in the remaining 11 wells of each plate was maintained in situ (not renewed milk) (NRM). After 24, 48, 72, 96 and 120 h, cell viability was determined by the tetrazolium salt-based colorimetric (MTT) assay. Data were statistically analyzed by Kruskal-Wallis, Scheffé and Mann-Whitney tests (a=5%). At 5 °C, only Milk-48 was significantly better than NRM. At 20 °C, NRM was more effective than Milk-12 and Milk-24 in all time periods. In relation to the temperature (5 °C or 20 °C), renewal of milk at 5 °C was better in maintaining cell viability than the renewal at 20 °C. In conclusion, the renewal of milk was able to increase its ability to maintain cell viability only when performed every 48 h in milk maintained at 5 °C. |
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Effect of Milk Renewal on Cell Viability In Vitro at Different Time Framesmilkperiodontal ligament fibroblastsstorage mediatooth avulsion.Abstract The purpose of this study was to evaluate if the renewal of milk as a storage medium, every 12, 24 and 48 h, is able to increase its ability to maintain human periodontal ligament fibroblasts (PDLF) viability over time. PDLF were soaked in Minimum Essential Medium at 37 °C (MEM-37) (positive control), tap water (Water) (negative control) and in skimmed milk (44 wells) at 5 °C and 20 °C. The skimmed milk was renewed every 12 h (Milk-12), 24 h (Milk-24) and 48 h (Milk-48) in 11 wells of each plate, and the milk in the remaining 11 wells of each plate was maintained in situ (not renewed milk) (NRM). After 24, 48, 72, 96 and 120 h, cell viability was determined by the tetrazolium salt-based colorimetric (MTT) assay. Data were statistically analyzed by Kruskal-Wallis, Scheffé and Mann-Whitney tests (a=5%). At 5 °C, only Milk-48 was significantly better than NRM. At 20 °C, NRM was more effective than Milk-12 and Milk-24 in all time periods. In relation to the temperature (5 °C or 20 °C), renewal of milk at 5 °C was better in maintaining cell viability than the renewal at 20 °C. In conclusion, the renewal of milk was able to increase its ability to maintain cell viability only when performed every 48 h in milk maintained at 5 °C.Fundação Odontológica de Ribeirão Preto2017-08-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-64402017000400435Brazilian Dental Journal v.28 n.4 2017reponame:Brazilian Dental Journalinstname:Fundação Odontológica de Ribeirão Preto (FUNORP)instacron:FUNORP10.1590/0103-6440201601164info:eu-repo/semantics/openAccessSouza,Beatriz Dulcineia Mendes deAlves,Ana Maria HeckeRibeiro,Dayane MachadoSantos,Luciane Geanini Pena dosSimões,Claudia Maria de OliveiraFelippe,Wilson TadeuFelippe,Mara Cristina Santoseng2017-11-08T00:00:00Zoai:scielo:S0103-64402017000400435Revistahttps://www.scielo.br/j/bdj/https://old.scielo.br/oai/scielo-oai.phpbdj@forp.usp.br||sergio@fosjc.unesp.br1806-47600103-6440opendoar:2017-11-08T00:00Brazilian Dental Journal - Fundação Odontológica de Ribeirão Preto (FUNORP)false |
dc.title.none.fl_str_mv |
Effect of Milk Renewal on Cell Viability In Vitro at Different Time Frames |
title |
Effect of Milk Renewal on Cell Viability In Vitro at Different Time Frames |
spellingShingle |
Effect of Milk Renewal on Cell Viability In Vitro at Different Time Frames Souza,Beatriz Dulcineia Mendes de milk periodontal ligament fibroblasts storage media tooth avulsion. |
title_short |
Effect of Milk Renewal on Cell Viability In Vitro at Different Time Frames |
title_full |
Effect of Milk Renewal on Cell Viability In Vitro at Different Time Frames |
title_fullStr |
Effect of Milk Renewal on Cell Viability In Vitro at Different Time Frames |
title_full_unstemmed |
Effect of Milk Renewal on Cell Viability In Vitro at Different Time Frames |
title_sort |
Effect of Milk Renewal on Cell Viability In Vitro at Different Time Frames |
author |
Souza,Beatriz Dulcineia Mendes de |
author_facet |
Souza,Beatriz Dulcineia Mendes de Alves,Ana Maria Hecke Ribeiro,Dayane Machado Santos,Luciane Geanini Pena dos Simões,Claudia Maria de Oliveira Felippe,Wilson Tadeu Felippe,Mara Cristina Santos |
author_role |
author |
author2 |
Alves,Ana Maria Hecke Ribeiro,Dayane Machado Santos,Luciane Geanini Pena dos Simões,Claudia Maria de Oliveira Felippe,Wilson Tadeu Felippe,Mara Cristina Santos |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Souza,Beatriz Dulcineia Mendes de Alves,Ana Maria Hecke Ribeiro,Dayane Machado Santos,Luciane Geanini Pena dos Simões,Claudia Maria de Oliveira Felippe,Wilson Tadeu Felippe,Mara Cristina Santos |
dc.subject.por.fl_str_mv |
milk periodontal ligament fibroblasts storage media tooth avulsion. |
topic |
milk periodontal ligament fibroblasts storage media tooth avulsion. |
description |
Abstract The purpose of this study was to evaluate if the renewal of milk as a storage medium, every 12, 24 and 48 h, is able to increase its ability to maintain human periodontal ligament fibroblasts (PDLF) viability over time. PDLF were soaked in Minimum Essential Medium at 37 °C (MEM-37) (positive control), tap water (Water) (negative control) and in skimmed milk (44 wells) at 5 °C and 20 °C. The skimmed milk was renewed every 12 h (Milk-12), 24 h (Milk-24) and 48 h (Milk-48) in 11 wells of each plate, and the milk in the remaining 11 wells of each plate was maintained in situ (not renewed milk) (NRM). After 24, 48, 72, 96 and 120 h, cell viability was determined by the tetrazolium salt-based colorimetric (MTT) assay. Data were statistically analyzed by Kruskal-Wallis, Scheffé and Mann-Whitney tests (a=5%). At 5 °C, only Milk-48 was significantly better than NRM. At 20 °C, NRM was more effective than Milk-12 and Milk-24 in all time periods. In relation to the temperature (5 °C or 20 °C), renewal of milk at 5 °C was better in maintaining cell viability than the renewal at 20 °C. In conclusion, the renewal of milk was able to increase its ability to maintain cell viability only when performed every 48 h in milk maintained at 5 °C. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-08-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-64402017000400435 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-64402017000400435 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/0103-6440201601164 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Fundação Odontológica de Ribeirão Preto |
publisher.none.fl_str_mv |
Fundação Odontológica de Ribeirão Preto |
dc.source.none.fl_str_mv |
Brazilian Dental Journal v.28 n.4 2017 reponame:Brazilian Dental Journal instname:Fundação Odontológica de Ribeirão Preto (FUNORP) instacron:FUNORP |
instname_str |
Fundação Odontológica de Ribeirão Preto (FUNORP) |
instacron_str |
FUNORP |
institution |
FUNORP |
reponame_str |
Brazilian Dental Journal |
collection |
Brazilian Dental Journal |
repository.name.fl_str_mv |
Brazilian Dental Journal - Fundação Odontológica de Ribeirão Preto (FUNORP) |
repository.mail.fl_str_mv |
bdj@forp.usp.br||sergio@fosjc.unesp.br |
_version_ |
1754204094813175808 |