The Effect of Cigarette Smoking And Low-Level Laser Irradiation in RANK/RANKL/OPG Expression
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Dental Journal |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-64402020000100057 |
Resumo: | Abstract The objective of this study was to investigate the effects of low-level laser therapy (LLLT) and cigarette smoke on alveolar socket osteoclastogenesis signaling after tooth extraction, in rats. Sixty male Wistar rats were randomly assigned to four groups with 15 animals each: Control Group (with right maxillary molar extraction - ME), Experimental I (with ME and LLLT), Experimental II (with ME and cigarette smoke) and Experimental III group (with ME, LLLT and cigarette smoke). Euthanasia was performed at 3, 7 and 14 days postoperative. qRT-PCR was used to evaluate expression of Tnfrsf11a (RANK), Tnfsf11 (Rankl) and Tnfrsf11b (OPG). Data were submitted to statistical analysis using two-way ANOVA followed by Bonferroni test (α=0.05). There was an upregulation of RANK, RANKL and OPG genes over all the time of healing in Exp I group compared to control group. Exp II group showed a decreased expression of all genes over time, whereas Exp III genes expression were higher than Exp II values but lower than Control and Exp I values over time. The results of this study concluded that the LLLT had a positive effect, whereas cigarette smoke had a negative effect on RANK, RANKL and OPG gene expression in bone remodeling process. |
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Brazilian Dental Journal |
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The Effect of Cigarette Smoking And Low-Level Laser Irradiation in RANK/RANKL/OPG Expressionbonetooth extractionbone remodelingRANKRANKLOPGAbstract The objective of this study was to investigate the effects of low-level laser therapy (LLLT) and cigarette smoke on alveolar socket osteoclastogenesis signaling after tooth extraction, in rats. Sixty male Wistar rats were randomly assigned to four groups with 15 animals each: Control Group (with right maxillary molar extraction - ME), Experimental I (with ME and LLLT), Experimental II (with ME and cigarette smoke) and Experimental III group (with ME, LLLT and cigarette smoke). Euthanasia was performed at 3, 7 and 14 days postoperative. qRT-PCR was used to evaluate expression of Tnfrsf11a (RANK), Tnfsf11 (Rankl) and Tnfrsf11b (OPG). Data were submitted to statistical analysis using two-way ANOVA followed by Bonferroni test (α=0.05). There was an upregulation of RANK, RANKL and OPG genes over all the time of healing in Exp I group compared to control group. Exp II group showed a decreased expression of all genes over time, whereas Exp III genes expression were higher than Exp II values but lower than Control and Exp I values over time. The results of this study concluded that the LLLT had a positive effect, whereas cigarette smoke had a negative effect on RANK, RANKL and OPG gene expression in bone remodeling process.Fundação Odontológica de Ribeirão Preto2020-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-64402020000100057Brazilian Dental Journal v.31 n.1 2020reponame:Brazilian Dental Journalinstname:Fundação Odontológica de Ribeirão Preto (FUNORP)instacron:FUNORP10.1590/0103-6440202002519info:eu-repo/semantics/openAccessRibeiro,Larissa Nogueira SoaresMonteiro,Patrícia MariaBarretto,Gabriel DessottiLuiz,Kelly GalisteuAlves,Sandra Yasuyo FukadaStuani,Maria Bernadete Sassoeng2020-03-02T00:00:00Zoai:scielo:S0103-64402020000100057Revistahttps://www.scielo.br/j/bdj/https://old.scielo.br/oai/scielo-oai.phpbdj@forp.usp.br||sergio@fosjc.unesp.br1806-47600103-6440opendoar:2020-03-02T00:00Brazilian Dental Journal - Fundação Odontológica de Ribeirão Preto (FUNORP)false |
dc.title.none.fl_str_mv |
The Effect of Cigarette Smoking And Low-Level Laser Irradiation in RANK/RANKL/OPG Expression |
title |
The Effect of Cigarette Smoking And Low-Level Laser Irradiation in RANK/RANKL/OPG Expression |
spellingShingle |
The Effect of Cigarette Smoking And Low-Level Laser Irradiation in RANK/RANKL/OPG Expression Ribeiro,Larissa Nogueira Soares bone tooth extraction bone remodeling RANK RANKL OPG |
title_short |
The Effect of Cigarette Smoking And Low-Level Laser Irradiation in RANK/RANKL/OPG Expression |
title_full |
The Effect of Cigarette Smoking And Low-Level Laser Irradiation in RANK/RANKL/OPG Expression |
title_fullStr |
The Effect of Cigarette Smoking And Low-Level Laser Irradiation in RANK/RANKL/OPG Expression |
title_full_unstemmed |
The Effect of Cigarette Smoking And Low-Level Laser Irradiation in RANK/RANKL/OPG Expression |
title_sort |
The Effect of Cigarette Smoking And Low-Level Laser Irradiation in RANK/RANKL/OPG Expression |
author |
Ribeiro,Larissa Nogueira Soares |
author_facet |
Ribeiro,Larissa Nogueira Soares Monteiro,Patrícia Maria Barretto,Gabriel Dessotti Luiz,Kelly Galisteu Alves,Sandra Yasuyo Fukada Stuani,Maria Bernadete Sasso |
author_role |
author |
author2 |
Monteiro,Patrícia Maria Barretto,Gabriel Dessotti Luiz,Kelly Galisteu Alves,Sandra Yasuyo Fukada Stuani,Maria Bernadete Sasso |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Ribeiro,Larissa Nogueira Soares Monteiro,Patrícia Maria Barretto,Gabriel Dessotti Luiz,Kelly Galisteu Alves,Sandra Yasuyo Fukada Stuani,Maria Bernadete Sasso |
dc.subject.por.fl_str_mv |
bone tooth extraction bone remodeling RANK RANKL OPG |
topic |
bone tooth extraction bone remodeling RANK RANKL OPG |
description |
Abstract The objective of this study was to investigate the effects of low-level laser therapy (LLLT) and cigarette smoke on alveolar socket osteoclastogenesis signaling after tooth extraction, in rats. Sixty male Wistar rats were randomly assigned to four groups with 15 animals each: Control Group (with right maxillary molar extraction - ME), Experimental I (with ME and LLLT), Experimental II (with ME and cigarette smoke) and Experimental III group (with ME, LLLT and cigarette smoke). Euthanasia was performed at 3, 7 and 14 days postoperative. qRT-PCR was used to evaluate expression of Tnfrsf11a (RANK), Tnfsf11 (Rankl) and Tnfrsf11b (OPG). Data were submitted to statistical analysis using two-way ANOVA followed by Bonferroni test (α=0.05). There was an upregulation of RANK, RANKL and OPG genes over all the time of healing in Exp I group compared to control group. Exp II group showed a decreased expression of all genes over time, whereas Exp III genes expression were higher than Exp II values but lower than Control and Exp I values over time. The results of this study concluded that the LLLT had a positive effect, whereas cigarette smoke had a negative effect on RANK, RANKL and OPG gene expression in bone remodeling process. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-64402020000100057 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-64402020000100057 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/0103-6440202002519 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Fundação Odontológica de Ribeirão Preto |
publisher.none.fl_str_mv |
Fundação Odontológica de Ribeirão Preto |
dc.source.none.fl_str_mv |
Brazilian Dental Journal v.31 n.1 2020 reponame:Brazilian Dental Journal instname:Fundação Odontológica de Ribeirão Preto (FUNORP) instacron:FUNORP |
instname_str |
Fundação Odontológica de Ribeirão Preto (FUNORP) |
instacron_str |
FUNORP |
institution |
FUNORP |
reponame_str |
Brazilian Dental Journal |
collection |
Brazilian Dental Journal |
repository.name.fl_str_mv |
Brazilian Dental Journal - Fundação Odontológica de Ribeirão Preto (FUNORP) |
repository.mail.fl_str_mv |
bdj@forp.usp.br||sergio@fosjc.unesp.br |
_version_ |
1754204095779962880 |