Beneficial Effects of Ethyl-Cyanoacrylate Coating Against Candida Albicans Biofilm Formation
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Dental Journal |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-64402019000300266 |
Resumo: | Abstract The aim of this study was to verify whether modifications made in a hard chairside reline resin by an ethyl-cyanoacrylate adhesive, ECA (Super Bonder®, Loctite, Itapevi, SP, Brazil) would be able to inhibit or reduce Candida albicans biofilm formation on its surface, comparing to a commercial surface sealant (BisCover®, Bisco, Schaumburg, USA). Reline resin specimens were fabricated and randomly divided into 6 groups (n=8): CG (control group), no surface treatment; ECA1, ECA coating on the surface before sterilization; ECA2, ECA coating after sterilization; ECA3, ECA incorporated in the resin bulk; DPE1, BisCover® coating before sterilization; DPE2, BisCover® coating after sterilization. Specimens were inoculated with C. albicans SC5314 (1x107 cells/mL) and incubated for 24 h. Then, the biofilm were stained with LIVE/DEAD® BaclightTM L7007 Kit and analyzed by Confocal Laser Scanning Microscopy. The images were evaluated by bioImageL® v.2.0 software and total biovolume (µm3), viable cells (%), and covered area (%) were calculated. Data were statistically analyzed by Kruskal-Wallis and Dunn tests (p<0.05). Results showed that ECA-coated groups presented better results, reducing C. albicans biofilm formation. Acquired images revealed that these groups (ECA1 and ECA2) presented a reduced number of cells, mostly in yeast form (less pathogenic), while the other groups presented higher number of cells, mostly in hyphae form (more pathogenic). Based on these findings, a beneficial effect of Super Bonder® coating reline resins surface could be demonstrated, suggesting a promising way to prevent fungal biofilm formation on dentures. |
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Brazilian Dental Journal |
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Beneficial Effects of Ethyl-Cyanoacrylate Coating Against Candida Albicans Biofilm Formationdenture linerscandida albicansconfocal microscopycyanoacrylatesbiofilm formationAbstract The aim of this study was to verify whether modifications made in a hard chairside reline resin by an ethyl-cyanoacrylate adhesive, ECA (Super Bonder®, Loctite, Itapevi, SP, Brazil) would be able to inhibit or reduce Candida albicans biofilm formation on its surface, comparing to a commercial surface sealant (BisCover®, Bisco, Schaumburg, USA). Reline resin specimens were fabricated and randomly divided into 6 groups (n=8): CG (control group), no surface treatment; ECA1, ECA coating on the surface before sterilization; ECA2, ECA coating after sterilization; ECA3, ECA incorporated in the resin bulk; DPE1, BisCover® coating before sterilization; DPE2, BisCover® coating after sterilization. Specimens were inoculated with C. albicans SC5314 (1x107 cells/mL) and incubated for 24 h. Then, the biofilm were stained with LIVE/DEAD® BaclightTM L7007 Kit and analyzed by Confocal Laser Scanning Microscopy. The images were evaluated by bioImageL® v.2.0 software and total biovolume (µm3), viable cells (%), and covered area (%) were calculated. Data were statistically analyzed by Kruskal-Wallis and Dunn tests (p<0.05). Results showed that ECA-coated groups presented better results, reducing C. albicans biofilm formation. Acquired images revealed that these groups (ECA1 and ECA2) presented a reduced number of cells, mostly in yeast form (less pathogenic), while the other groups presented higher number of cells, mostly in hyphae form (more pathogenic). Based on these findings, a beneficial effect of Super Bonder® coating reline resins surface could be demonstrated, suggesting a promising way to prevent fungal biofilm formation on dentures.Fundação Odontológica de Ribeirão Preto2019-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-64402019000300266Brazilian Dental Journal v.30 n.3 2019reponame:Brazilian Dental Journalinstname:Fundação Odontológica de Ribeirão Preto (FUNORP)instacron:FUNORP10.1590/0103-6440201901953info:eu-repo/semantics/openAccessTávora,Flora Freitas FernandesChocano,Ana Paula ChappuisOliveira,Denise Gusmão dePereira,Jefferson RicardoAlmeida,Ricardo SergioNeppelenbroek,Karin HermanaPorto,Vinícius Carvalhoeng2019-06-06T00:00:00Zoai:scielo:S0103-64402019000300266Revistahttps://www.scielo.br/j/bdj/https://old.scielo.br/oai/scielo-oai.phpbdj@forp.usp.br||sergio@fosjc.unesp.br1806-47600103-6440opendoar:2019-06-06T00:00Brazilian Dental Journal - Fundação Odontológica de Ribeirão Preto (FUNORP)false |
dc.title.none.fl_str_mv |
Beneficial Effects of Ethyl-Cyanoacrylate Coating Against Candida Albicans Biofilm Formation |
title |
Beneficial Effects of Ethyl-Cyanoacrylate Coating Against Candida Albicans Biofilm Formation |
spellingShingle |
Beneficial Effects of Ethyl-Cyanoacrylate Coating Against Candida Albicans Biofilm Formation Távora,Flora Freitas Fernandes denture liners candida albicans confocal microscopy cyanoacrylates biofilm formation |
title_short |
Beneficial Effects of Ethyl-Cyanoacrylate Coating Against Candida Albicans Biofilm Formation |
title_full |
Beneficial Effects of Ethyl-Cyanoacrylate Coating Against Candida Albicans Biofilm Formation |
title_fullStr |
Beneficial Effects of Ethyl-Cyanoacrylate Coating Against Candida Albicans Biofilm Formation |
title_full_unstemmed |
Beneficial Effects of Ethyl-Cyanoacrylate Coating Against Candida Albicans Biofilm Formation |
title_sort |
Beneficial Effects of Ethyl-Cyanoacrylate Coating Against Candida Albicans Biofilm Formation |
author |
Távora,Flora Freitas Fernandes |
author_facet |
Távora,Flora Freitas Fernandes Chocano,Ana Paula Chappuis Oliveira,Denise Gusmão de Pereira,Jefferson Ricardo Almeida,Ricardo Sergio Neppelenbroek,Karin Hermana Porto,Vinícius Carvalho |
author_role |
author |
author2 |
Chocano,Ana Paula Chappuis Oliveira,Denise Gusmão de Pereira,Jefferson Ricardo Almeida,Ricardo Sergio Neppelenbroek,Karin Hermana Porto,Vinícius Carvalho |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Távora,Flora Freitas Fernandes Chocano,Ana Paula Chappuis Oliveira,Denise Gusmão de Pereira,Jefferson Ricardo Almeida,Ricardo Sergio Neppelenbroek,Karin Hermana Porto,Vinícius Carvalho |
dc.subject.por.fl_str_mv |
denture liners candida albicans confocal microscopy cyanoacrylates biofilm formation |
topic |
denture liners candida albicans confocal microscopy cyanoacrylates biofilm formation |
description |
Abstract The aim of this study was to verify whether modifications made in a hard chairside reline resin by an ethyl-cyanoacrylate adhesive, ECA (Super Bonder®, Loctite, Itapevi, SP, Brazil) would be able to inhibit or reduce Candida albicans biofilm formation on its surface, comparing to a commercial surface sealant (BisCover®, Bisco, Schaumburg, USA). Reline resin specimens were fabricated and randomly divided into 6 groups (n=8): CG (control group), no surface treatment; ECA1, ECA coating on the surface before sterilization; ECA2, ECA coating after sterilization; ECA3, ECA incorporated in the resin bulk; DPE1, BisCover® coating before sterilization; DPE2, BisCover® coating after sterilization. Specimens were inoculated with C. albicans SC5314 (1x107 cells/mL) and incubated for 24 h. Then, the biofilm were stained with LIVE/DEAD® BaclightTM L7007 Kit and analyzed by Confocal Laser Scanning Microscopy. The images were evaluated by bioImageL® v.2.0 software and total biovolume (µm3), viable cells (%), and covered area (%) were calculated. Data were statistically analyzed by Kruskal-Wallis and Dunn tests (p<0.05). Results showed that ECA-coated groups presented better results, reducing C. albicans biofilm formation. Acquired images revealed that these groups (ECA1 and ECA2) presented a reduced number of cells, mostly in yeast form (less pathogenic), while the other groups presented higher number of cells, mostly in hyphae form (more pathogenic). Based on these findings, a beneficial effect of Super Bonder® coating reline resins surface could be demonstrated, suggesting a promising way to prevent fungal biofilm formation on dentures. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-06-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-64402019000300266 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-64402019000300266 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/0103-6440201901953 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Fundação Odontológica de Ribeirão Preto |
publisher.none.fl_str_mv |
Fundação Odontológica de Ribeirão Preto |
dc.source.none.fl_str_mv |
Brazilian Dental Journal v.30 n.3 2019 reponame:Brazilian Dental Journal instname:Fundação Odontológica de Ribeirão Preto (FUNORP) instacron:FUNORP |
instname_str |
Fundação Odontológica de Ribeirão Preto (FUNORP) |
instacron_str |
FUNORP |
institution |
FUNORP |
reponame_str |
Brazilian Dental Journal |
collection |
Brazilian Dental Journal |
repository.name.fl_str_mv |
Brazilian Dental Journal - Fundação Odontológica de Ribeirão Preto (FUNORP) |
repository.mail.fl_str_mv |
bdj@forp.usp.br||sergio@fosjc.unesp.br |
_version_ |
1754204095673008128 |