M1 and M2 macrophages phenotypes modulation after stimuli with materials used in endodontic treatment
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Dental Journal |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-64402021000300032 |
Resumo: | Abstract The aim of this study was to evaluate the M1 and M2 macrophage modulation after stimuli with different materials used during endodontic treatment. In bone marrow-derived macrophage cell culture, from males C57BL/6 wild-type (WT) mice, gene expression analysis of markers to M1 and M2 macrophages was performed by qRT-PCR (Cxcl10, CxCL9, iNOS, Arg1, Chil3, Retnla and MRC1) and cytokine quantification by Luminex® (GM-CSF, IL-10, IL-6, IL-1β and TNF-α) after exposure to the five endodontic sealers: AH Plus, Sealapex Xpress, Endosequence BC Sealer, BioRoot RCS and a calcium hydroxide-based paste. For normal values, ANOVA test was used, followed by Tukey post-test. For non-normal values, the Kruskall-Wallis test was used. BioRootTM RCS and EndoSequence BC SealerTM stimulated the highest expression of markers for M1 macrophages, while calcium hydroxide-based paste stimulated the lowest expression of these gene markers. For M2 protein markers, BioRootTM RCS presented the highest stimulation while calcium hydroxide-based paste also presented the lowest stimulation. It was concluded that all the evaluated filling materials increased the genetic expression of pro- and anti-inflammatory markers: TNF-α and IL-10 respectively. The others proinflammatory mediators showed differences against the filling materials. However, this process did not induce the inflammatory response polarization, resulting in a hybrid macrophage. |
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Brazilian Dental Journal |
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M1 and M2 macrophages phenotypes modulation after stimuli with materials used in endodontic treatmentmacrophagescytokinesperiapical periodontitisroot canal obturationcalcium hydroxideAbstract The aim of this study was to evaluate the M1 and M2 macrophage modulation after stimuli with different materials used during endodontic treatment. In bone marrow-derived macrophage cell culture, from males C57BL/6 wild-type (WT) mice, gene expression analysis of markers to M1 and M2 macrophages was performed by qRT-PCR (Cxcl10, CxCL9, iNOS, Arg1, Chil3, Retnla and MRC1) and cytokine quantification by Luminex® (GM-CSF, IL-10, IL-6, IL-1β and TNF-α) after exposure to the five endodontic sealers: AH Plus, Sealapex Xpress, Endosequence BC Sealer, BioRoot RCS and a calcium hydroxide-based paste. For normal values, ANOVA test was used, followed by Tukey post-test. For non-normal values, the Kruskall-Wallis test was used. BioRootTM RCS and EndoSequence BC SealerTM stimulated the highest expression of markers for M1 macrophages, while calcium hydroxide-based paste stimulated the lowest expression of these gene markers. For M2 protein markers, BioRootTM RCS presented the highest stimulation while calcium hydroxide-based paste also presented the lowest stimulation. It was concluded that all the evaluated filling materials increased the genetic expression of pro- and anti-inflammatory markers: TNF-α and IL-10 respectively. The others proinflammatory mediators showed differences against the filling materials. However, this process did not induce the inflammatory response polarization, resulting in a hybrid macrophage.Fundação Odontológica de Ribeirão Preto2021-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-64402021000300032Brazilian Dental Journal v.32 n.3 2021reponame:Brazilian Dental Journalinstname:Fundação Odontológica de Ribeirão Preto (FUNORP)instacron:FUNORP10.1590/0103-6440202104038info:eu-repo/semantics/openAccessDa Silva,Raquel Assed BezerraDa Silva,Léa Assed BezerraGabriel-Junior,Edson AlvesSorgi,Carlos ArterioFaccioli,Lúcia HelenaMassoni,Vivian VicentinNelson-Filho,PauloPucinelli,Carolina Maschiettoeng2021-11-05T00:00:00Zoai:scielo:S0103-64402021000300032Revistahttps://www.scielo.br/j/bdj/https://old.scielo.br/oai/scielo-oai.phpbdj@forp.usp.br||sergio@fosjc.unesp.br1806-47600103-6440opendoar:2021-11-05T00:00Brazilian Dental Journal - Fundação Odontológica de Ribeirão Preto (FUNORP)false |
dc.title.none.fl_str_mv |
M1 and M2 macrophages phenotypes modulation after stimuli with materials used in endodontic treatment |
title |
M1 and M2 macrophages phenotypes modulation after stimuli with materials used in endodontic treatment |
spellingShingle |
M1 and M2 macrophages phenotypes modulation after stimuli with materials used in endodontic treatment Da Silva,Raquel Assed Bezerra macrophages cytokines periapical periodontitis root canal obturation calcium hydroxide |
title_short |
M1 and M2 macrophages phenotypes modulation after stimuli with materials used in endodontic treatment |
title_full |
M1 and M2 macrophages phenotypes modulation after stimuli with materials used in endodontic treatment |
title_fullStr |
M1 and M2 macrophages phenotypes modulation after stimuli with materials used in endodontic treatment |
title_full_unstemmed |
M1 and M2 macrophages phenotypes modulation after stimuli with materials used in endodontic treatment |
title_sort |
M1 and M2 macrophages phenotypes modulation after stimuli with materials used in endodontic treatment |
author |
Da Silva,Raquel Assed Bezerra |
author_facet |
Da Silva,Raquel Assed Bezerra Da Silva,Léa Assed Bezerra Gabriel-Junior,Edson Alves Sorgi,Carlos Arterio Faccioli,Lúcia Helena Massoni,Vivian Vicentin Nelson-Filho,Paulo Pucinelli,Carolina Maschietto |
author_role |
author |
author2 |
Da Silva,Léa Assed Bezerra Gabriel-Junior,Edson Alves Sorgi,Carlos Arterio Faccioli,Lúcia Helena Massoni,Vivian Vicentin Nelson-Filho,Paulo Pucinelli,Carolina Maschietto |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Da Silva,Raquel Assed Bezerra Da Silva,Léa Assed Bezerra Gabriel-Junior,Edson Alves Sorgi,Carlos Arterio Faccioli,Lúcia Helena Massoni,Vivian Vicentin Nelson-Filho,Paulo Pucinelli,Carolina Maschietto |
dc.subject.por.fl_str_mv |
macrophages cytokines periapical periodontitis root canal obturation calcium hydroxide |
topic |
macrophages cytokines periapical periodontitis root canal obturation calcium hydroxide |
description |
Abstract The aim of this study was to evaluate the M1 and M2 macrophage modulation after stimuli with different materials used during endodontic treatment. In bone marrow-derived macrophage cell culture, from males C57BL/6 wild-type (WT) mice, gene expression analysis of markers to M1 and M2 macrophages was performed by qRT-PCR (Cxcl10, CxCL9, iNOS, Arg1, Chil3, Retnla and MRC1) and cytokine quantification by Luminex® (GM-CSF, IL-10, IL-6, IL-1β and TNF-α) after exposure to the five endodontic sealers: AH Plus, Sealapex Xpress, Endosequence BC Sealer, BioRoot RCS and a calcium hydroxide-based paste. For normal values, ANOVA test was used, followed by Tukey post-test. For non-normal values, the Kruskall-Wallis test was used. BioRootTM RCS and EndoSequence BC SealerTM stimulated the highest expression of markers for M1 macrophages, while calcium hydroxide-based paste stimulated the lowest expression of these gene markers. For M2 protein markers, BioRootTM RCS presented the highest stimulation while calcium hydroxide-based paste also presented the lowest stimulation. It was concluded that all the evaluated filling materials increased the genetic expression of pro- and anti-inflammatory markers: TNF-α and IL-10 respectively. The others proinflammatory mediators showed differences against the filling materials. However, this process did not induce the inflammatory response polarization, resulting in a hybrid macrophage. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-06-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-64402021000300032 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-64402021000300032 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/0103-6440202104038 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Fundação Odontológica de Ribeirão Preto |
publisher.none.fl_str_mv |
Fundação Odontológica de Ribeirão Preto |
dc.source.none.fl_str_mv |
Brazilian Dental Journal v.32 n.3 2021 reponame:Brazilian Dental Journal instname:Fundação Odontológica de Ribeirão Preto (FUNORP) instacron:FUNORP |
instname_str |
Fundação Odontológica de Ribeirão Preto (FUNORP) |
instacron_str |
FUNORP |
institution |
FUNORP |
reponame_str |
Brazilian Dental Journal |
collection |
Brazilian Dental Journal |
repository.name.fl_str_mv |
Brazilian Dental Journal - Fundação Odontológica de Ribeirão Preto (FUNORP) |
repository.mail.fl_str_mv |
bdj@forp.usp.br||sergio@fosjc.unesp.br |
_version_ |
1754204096245530624 |