Role of hydrophobic extracellular polysaccharide of Aulacoseira granulata (Bacillariophyceae) on aggregate formation in a turbulent and hypereutrophic reservoir
Autor(a) principal: | |
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Data de Publicação: | 2008 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da FURG (RI FURG) |
Texto Completo: | http://repositorio.furg.br/handle/1/713 |
Resumo: | The highest density of aggregates (1.4 3 103 L21) in the hypereutrophic Barra Bonita Reservoir (mean depth of 10.2 m) coincided with the peak population density of the diatom Aulacoseira granulata (2.3 3 106 chains L21), which was present in 94% of the aggregates for 22 consecutive months. To investigate why this diatom, which produces very small amounts of free extracellular polysaccharide (EPS) and transparent exopolymer particles (TEP), occurs so frequently in natural aggregates, we carried out aggregation experiments with cultures of A. granulata with and without its free EPS and TEP. Stickiness (a) was higher in exponential than stationary growth, either with or without EPS, but the highest value was obtained in cultures without EPS (0.37). During the stationary growth phase, a decreased, both with and without EPS. Replacement of natural polymer carbohydrates by A. granulata free EPS (.14 kiloDalton [kD] , 0.4 mm), or adding cultured diatom to reservoir water, produced aggregates larger than the natural ones. The role of EPS in these aggregation characteristics was investigated by analyzing its monosaccharide composition. The proportion of terminal monosaccharides significant in the aggregation was 74.5%, 56.1% of them consisting of hydrophobic deoxy-sugars fucose and rhamnose which increase the stickiness. Early in the growth, only hydrophobic adhering films are responsible for fast aggregation by chain-to-chain collision. With aging cells, aggregation was mainly by the trapping effect of free EPS and TEP, both formed by released adherent films. We hypothesize that aggregates have an important role in seeding A. granulata in environments with significant advective transport, such as reservoirs. |
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Vieira, Armando Augusto HenriquesOrtolano, Pedro Ivo CoelhoGiroldo, DaniloOliveira, Maria José DellamanoBittar, Thais BeraldoLombardi, Ana TeresaSartori, Antonio Luiz2011-06-17T03:46:57Z2011-06-17T03:46:57Z2008VIEIRA, Armando Augusto Henriques et al. Role of hydrophobic extracellular polysaccharide of Aulacoseira granulata (Bacillariophyceae) on aggregate formation in a turbulent and hypereutrophic reservoir. Limnology and Oceanography, Canadá, v. 53, n. 5, p. 1887-1899, 2008. Disponível em: <http://www.aslo.org/lo/toc/vol_53/issue_5/1887.pdf>. Acesso em: 14 jun. 2011.0024-3590http://repositorio.furg.br/handle/1/713The highest density of aggregates (1.4 3 103 L21) in the hypereutrophic Barra Bonita Reservoir (mean depth of 10.2 m) coincided with the peak population density of the diatom Aulacoseira granulata (2.3 3 106 chains L21), which was present in 94% of the aggregates for 22 consecutive months. To investigate why this diatom, which produces very small amounts of free extracellular polysaccharide (EPS) and transparent exopolymer particles (TEP), occurs so frequently in natural aggregates, we carried out aggregation experiments with cultures of A. granulata with and without its free EPS and TEP. Stickiness (a) was higher in exponential than stationary growth, either with or without EPS, but the highest value was obtained in cultures without EPS (0.37). During the stationary growth phase, a decreased, both with and without EPS. Replacement of natural polymer carbohydrates by A. granulata free EPS (.14 kiloDalton [kD] , 0.4 mm), or adding cultured diatom to reservoir water, produced aggregates larger than the natural ones. The role of EPS in these aggregation characteristics was investigated by analyzing its monosaccharide composition. The proportion of terminal monosaccharides significant in the aggregation was 74.5%, 56.1% of them consisting of hydrophobic deoxy-sugars fucose and rhamnose which increase the stickiness. Early in the growth, only hydrophobic adhering films are responsible for fast aggregation by chain-to-chain collision. With aging cells, aggregation was mainly by the trapping effect of free EPS and TEP, both formed by released adherent films. We hypothesize that aggregates have an important role in seeding A. granulata in environments with significant advective transport, such as reservoirs.engRole of hydrophobic extracellular polysaccharide of Aulacoseira granulata (Bacillariophyceae) on aggregate formation in a turbulent and hypereutrophic reservoirinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FURG (RI FURG)instname:Universidade Federal do Rio Grande (FURG)instacron:FURGORIGINALRole of hydrophobic extracellular polysacharide of aulacoseira granulata Bacillariophyceae on aggregate formation in a turbulent and hypereutrophic reservoir.pdfRole of hydrophobic extracellular polysacharide of aulacoseira granulata Bacillariophyceae on aggregate formation in a turbulent and hypereutrophic reservoir.pdfapplication/pdf1489171https://repositorio.furg.br/bitstream/1/713/1/Role%20of%20hydrophobic%20extracellular%20polysacharide%20of%20aulacoseira%20granulata%20Bacillariophyceae%20on%20aggregate%20formation%20in%20a%20turbulent%20and%20hypereutrophic%20reservoir.pdf5a43067465fcc3c5f6b8c7e1788e6cacMD51open accessLICENSElicense.txtlicense.txttext/plain; charset=utf-81724https://repositorio.furg.br/bitstream/1/713/2/license.txt5b92b9704b4f13242d70e45ddef35a68MD52open access1/7132011-07-29 15:39:45.321open accessoai:repositorio.furg.br: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Repositório InstitucionalPUBhttps://repositorio.furg.br/oai/request || http://200.19.254.174/oai/requestopendoar:2011-07-29T18:39:45Repositório Institucional da FURG (RI FURG) - Universidade Federal do Rio Grande (FURG)false |
dc.title.pt_BR.fl_str_mv |
Role of hydrophobic extracellular polysaccharide of Aulacoseira granulata (Bacillariophyceae) on aggregate formation in a turbulent and hypereutrophic reservoir |
title |
Role of hydrophobic extracellular polysaccharide of Aulacoseira granulata (Bacillariophyceae) on aggregate formation in a turbulent and hypereutrophic reservoir |
spellingShingle |
Role of hydrophobic extracellular polysaccharide of Aulacoseira granulata (Bacillariophyceae) on aggregate formation in a turbulent and hypereutrophic reservoir Vieira, Armando Augusto Henriques |
title_short |
Role of hydrophobic extracellular polysaccharide of Aulacoseira granulata (Bacillariophyceae) on aggregate formation in a turbulent and hypereutrophic reservoir |
title_full |
Role of hydrophobic extracellular polysaccharide of Aulacoseira granulata (Bacillariophyceae) on aggregate formation in a turbulent and hypereutrophic reservoir |
title_fullStr |
Role of hydrophobic extracellular polysaccharide of Aulacoseira granulata (Bacillariophyceae) on aggregate formation in a turbulent and hypereutrophic reservoir |
title_full_unstemmed |
Role of hydrophobic extracellular polysaccharide of Aulacoseira granulata (Bacillariophyceae) on aggregate formation in a turbulent and hypereutrophic reservoir |
title_sort |
Role of hydrophobic extracellular polysaccharide of Aulacoseira granulata (Bacillariophyceae) on aggregate formation in a turbulent and hypereutrophic reservoir |
author |
Vieira, Armando Augusto Henriques |
author_facet |
Vieira, Armando Augusto Henriques Ortolano, Pedro Ivo Coelho Giroldo, Danilo Oliveira, Maria José Dellamano Bittar, Thais Beraldo Lombardi, Ana Teresa Sartori, Antonio Luiz |
author_role |
author |
author2 |
Ortolano, Pedro Ivo Coelho Giroldo, Danilo Oliveira, Maria José Dellamano Bittar, Thais Beraldo Lombardi, Ana Teresa Sartori, Antonio Luiz |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Vieira, Armando Augusto Henriques Ortolano, Pedro Ivo Coelho Giroldo, Danilo Oliveira, Maria José Dellamano Bittar, Thais Beraldo Lombardi, Ana Teresa Sartori, Antonio Luiz |
description |
The highest density of aggregates (1.4 3 103 L21) in the hypereutrophic Barra Bonita Reservoir (mean depth of 10.2 m) coincided with the peak population density of the diatom Aulacoseira granulata (2.3 3 106 chains L21), which was present in 94% of the aggregates for 22 consecutive months. To investigate why this diatom, which produces very small amounts of free extracellular polysaccharide (EPS) and transparent exopolymer particles (TEP), occurs so frequently in natural aggregates, we carried out aggregation experiments with cultures of A. granulata with and without its free EPS and TEP. Stickiness (a) was higher in exponential than stationary growth, either with or without EPS, but the highest value was obtained in cultures without EPS (0.37). During the stationary growth phase, a decreased, both with and without EPS. Replacement of natural polymer carbohydrates by A. granulata free EPS (.14 kiloDalton [kD] , 0.4 mm), or adding cultured diatom to reservoir water, produced aggregates larger than the natural ones. The role of EPS in these aggregation characteristics was investigated by analyzing its monosaccharide composition. The proportion of terminal monosaccharides significant in the aggregation was 74.5%, 56.1% of them consisting of hydrophobic deoxy-sugars fucose and rhamnose which increase the stickiness. Early in the growth, only hydrophobic adhering films are responsible for fast aggregation by chain-to-chain collision. With aging cells, aggregation was mainly by the trapping effect of free EPS and TEP, both formed by released adherent films. We hypothesize that aggregates have an important role in seeding A. granulata in environments with significant advective transport, such as reservoirs. |
publishDate |
2008 |
dc.date.issued.fl_str_mv |
2008 |
dc.date.accessioned.fl_str_mv |
2011-06-17T03:46:57Z |
dc.date.available.fl_str_mv |
2011-06-17T03:46:57Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
VIEIRA, Armando Augusto Henriques et al. Role of hydrophobic extracellular polysaccharide of Aulacoseira granulata (Bacillariophyceae) on aggregate formation in a turbulent and hypereutrophic reservoir. Limnology and Oceanography, Canadá, v. 53, n. 5, p. 1887-1899, 2008. Disponível em: <http://www.aslo.org/lo/toc/vol_53/issue_5/1887.pdf>. Acesso em: 14 jun. 2011. |
dc.identifier.uri.fl_str_mv |
http://repositorio.furg.br/handle/1/713 |
dc.identifier.issn.none.fl_str_mv |
0024-3590 |
identifier_str_mv |
VIEIRA, Armando Augusto Henriques et al. Role of hydrophobic extracellular polysaccharide of Aulacoseira granulata (Bacillariophyceae) on aggregate formation in a turbulent and hypereutrophic reservoir. Limnology and Oceanography, Canadá, v. 53, n. 5, p. 1887-1899, 2008. Disponível em: <http://www.aslo.org/lo/toc/vol_53/issue_5/1887.pdf>. Acesso em: 14 jun. 2011. 0024-3590 |
url |
http://repositorio.furg.br/handle/1/713 |
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eng |
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eng |
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FURG |
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