The role of N-acetylcysteine in the lung remote injury after hepatic ischemia and reperfusion in rabbits

Detalhes bibliográficos
Autor(a) principal: Castro, Angela Pötter de
Data de Publicação: 2012
Outros Autores: Castro Junior, Miguel Angelo Martins de, Lauz, Susi, Facin, Emilio, Simões, Manuel de Jesus, Fagundes, Djalma José
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FURG (RI FURG)
Texto Completo: http://repositorio.furg.br/handle/1/4070
Resumo: PURPOSE: To study the lesions in the lung of rabbits caused by ischemia/reperfusion hepatic (I/R) after the use of N-acetyl-cysteine (NAC). METHODS: Twenty-four rabbits distributed in two groups: control group GI (n = 12) 5% glucose solution and experiment group GII (n = 12) NAC. The animals were pre-anesthetized with 1% acepromazine maleate and anesthetized with ketamine 10% and 2% xylazine intramuscularly. The GI and GII were given glucose solution intravenously or NAC 15min before occlusion of the hepatic pedicle (30 min). After the period of reperfusion of 24h (n = 6) or 48h (n = 6), liver and lung samples were collected for histology and immunohistochemistry to assess the impairment of cell. RESULTS: The animals of GII and GII-24h-48h showed parenchyma liver close to normal, when using NAC. The GII and GII-24h-48h showed lower thickness of alveolar cells that GI and GI-24h-48h. The expression of caspase 3 in lung cells GII presented smaller value compared to the GI group. CONCLUSION: N-acetyl-cysteine administered 15min prior to the injury ischemia/reperfusion had a significant protective role by minimizing lung injury and apoptotic morphology in the period observed.
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spelling The role of N-acetylcysteine in the lung remote injury after hepatic ischemia and reperfusion in rabbitsO papel da N-acetil-cisteína nas lesões pulmonares provocadas à distância pela isquemia e reperfusão hepática em coelhos.AcetylcysteineLungReperfusionIschemiaLiverRabbitsAcetilcisteínaPulmãoReperfusãoIsquemiaFígadoCoelhosPURPOSE: To study the lesions in the lung of rabbits caused by ischemia/reperfusion hepatic (I/R) after the use of N-acetyl-cysteine (NAC). METHODS: Twenty-four rabbits distributed in two groups: control group GI (n = 12) 5% glucose solution and experiment group GII (n = 12) NAC. The animals were pre-anesthetized with 1% acepromazine maleate and anesthetized with ketamine 10% and 2% xylazine intramuscularly. The GI and GII were given glucose solution intravenously or NAC 15min before occlusion of the hepatic pedicle (30 min). After the period of reperfusion of 24h (n = 6) or 48h (n = 6), liver and lung samples were collected for histology and immunohistochemistry to assess the impairment of cell. RESULTS: The animals of GII and GII-24h-48h showed parenchyma liver close to normal, when using NAC. The GII and GII-24h-48h showed lower thickness of alveolar cells that GI and GI-24h-48h. The expression of caspase 3 in lung cells GII presented smaller value compared to the GI group. CONCLUSION: N-acetyl-cysteine administered 15min prior to the injury ischemia/reperfusion had a significant protective role by minimizing lung injury and apoptotic morphology in the period observed.OBJETIVO: Estudar as lesões no fígado e no pulmão de coelhos, provocadas pela isquemia/reperfusão hepática (I/R) moduladas pelo uso da N-acetil-cisteína (NAC). MÉTODOS: Vinte e quatro coelhos distribuídos em dois grupos: Grupo controle GI (n=12) solução de glicose 5% e Grupo experimento GII (n=12) NAC. Os animais foram pré-anestesiados com maleato de acepromazina 1% e anestesiados com cloridrato de quetamina 10% e xilazina 2% via intramuscular. Os grupos GI e GII receberam solução glicosada ou NAC respectivamente via endovenosa 15min antes da oclusão do pedículo hepático (30 min). Após iniciou-se o período de reperfusão por 24h (n=6) ou 48h (n=6), terminada a reperfusão, amostras do fígado e pulmão foram coletadas para a histologia e imunoistoquímica para avaliar o comprometimento celular. RESULTADOS: Os animais do grupo GII-24h e GII-48h apresentaram arquitetura do parênquima hepático próximo do normal, quando se utilizou NAC. Os grupos GII-24h e GII-48h apresentaram menor espessura das células alveolares que os grupos GI-24h e GI-48h. A expressão da caspase 3 nas células pulmonares do grupo GII, apresentou valor menor comparada ao grupo GI. CONCLUSÃO: A N-acetil-cisteína administrada 15min prévios a lesão da isquemia/reperfusão teve um papel protetor significativo minimizando as lesões morfológicas e apoptóticas pulmonares nos períodos observados.2013-10-19T00:47:22Z2013-10-19T00:47:22Z2012info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfCASTRO, Angela Potter de et al. he role of N-acetylcysteine in the lung remote injury after hepatic ischemia and reperfusion in rabbits. Acta Cirúrgica Brasileira, v. 27, n. 1, p. 49-55, 2012. Disponível em: <http://www.scielo.br/pdf/acb/v27n1/a09v27n1.pdf>. Acesso em: out. 2013.http://repositorio.furg.br/handle/1/4070engCastro, Angela Pötter deCastro Junior, Miguel Angelo Martins deLauz, SusiFacin, EmilioSimões, Manuel de JesusFagundes, Djalma Joséinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FURG (RI FURG)instname:Universidade Federal do Rio Grande (FURG)instacron:FURG2014-09-04T15:04:08Zoai:repositorio.furg.br:1/4070Repositório InstitucionalPUBhttps://repositorio.furg.br/oai/request || http://200.19.254.174/oai/requestopendoar:2014-09-04T15:04:08Repositório Institucional da FURG (RI FURG) - Universidade Federal do Rio Grande (FURG)false
dc.title.none.fl_str_mv The role of N-acetylcysteine in the lung remote injury after hepatic ischemia and reperfusion in rabbits
O papel da N-acetil-cisteína nas lesões pulmonares provocadas à distância pela isquemia e reperfusão hepática em coelhos.
title The role of N-acetylcysteine in the lung remote injury after hepatic ischemia and reperfusion in rabbits
spellingShingle The role of N-acetylcysteine in the lung remote injury after hepatic ischemia and reperfusion in rabbits
Castro, Angela Pötter de
Acetylcysteine
Lung
Reperfusion
Ischemia
Liver
Rabbits
Acetilcisteína
Pulmão
Reperfusão
Isquemia
Fígado
Coelhos
title_short The role of N-acetylcysteine in the lung remote injury after hepatic ischemia and reperfusion in rabbits
title_full The role of N-acetylcysteine in the lung remote injury after hepatic ischemia and reperfusion in rabbits
title_fullStr The role of N-acetylcysteine in the lung remote injury after hepatic ischemia and reperfusion in rabbits
title_full_unstemmed The role of N-acetylcysteine in the lung remote injury after hepatic ischemia and reperfusion in rabbits
title_sort The role of N-acetylcysteine in the lung remote injury after hepatic ischemia and reperfusion in rabbits
author Castro, Angela Pötter de
author_facet Castro, Angela Pötter de
Castro Junior, Miguel Angelo Martins de
Lauz, Susi
Facin, Emilio
Simões, Manuel de Jesus
Fagundes, Djalma José
author_role author
author2 Castro Junior, Miguel Angelo Martins de
Lauz, Susi
Facin, Emilio
Simões, Manuel de Jesus
Fagundes, Djalma José
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Castro, Angela Pötter de
Castro Junior, Miguel Angelo Martins de
Lauz, Susi
Facin, Emilio
Simões, Manuel de Jesus
Fagundes, Djalma José
dc.subject.por.fl_str_mv Acetylcysteine
Lung
Reperfusion
Ischemia
Liver
Rabbits
Acetilcisteína
Pulmão
Reperfusão
Isquemia
Fígado
Coelhos
topic Acetylcysteine
Lung
Reperfusion
Ischemia
Liver
Rabbits
Acetilcisteína
Pulmão
Reperfusão
Isquemia
Fígado
Coelhos
description PURPOSE: To study the lesions in the lung of rabbits caused by ischemia/reperfusion hepatic (I/R) after the use of N-acetyl-cysteine (NAC). METHODS: Twenty-four rabbits distributed in two groups: control group GI (n = 12) 5% glucose solution and experiment group GII (n = 12) NAC. The animals were pre-anesthetized with 1% acepromazine maleate and anesthetized with ketamine 10% and 2% xylazine intramuscularly. The GI and GII were given glucose solution intravenously or NAC 15min before occlusion of the hepatic pedicle (30 min). After the period of reperfusion of 24h (n = 6) or 48h (n = 6), liver and lung samples were collected for histology and immunohistochemistry to assess the impairment of cell. RESULTS: The animals of GII and GII-24h-48h showed parenchyma liver close to normal, when using NAC. The GII and GII-24h-48h showed lower thickness of alveolar cells that GI and GI-24h-48h. The expression of caspase 3 in lung cells GII presented smaller value compared to the GI group. CONCLUSION: N-acetyl-cysteine administered 15min prior to the injury ischemia/reperfusion had a significant protective role by minimizing lung injury and apoptotic morphology in the period observed.
publishDate 2012
dc.date.none.fl_str_mv 2012
2013-10-19T00:47:22Z
2013-10-19T00:47:22Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv CASTRO, Angela Potter de et al. he role of N-acetylcysteine in the lung remote injury after hepatic ischemia and reperfusion in rabbits. Acta Cirúrgica Brasileira, v. 27, n. 1, p. 49-55, 2012. Disponível em: <http://www.scielo.br/pdf/acb/v27n1/a09v27n1.pdf>. Acesso em: out. 2013.
http://repositorio.furg.br/handle/1/4070
identifier_str_mv CASTRO, Angela Potter de et al. he role of N-acetylcysteine in the lung remote injury after hepatic ischemia and reperfusion in rabbits. Acta Cirúrgica Brasileira, v. 27, n. 1, p. 49-55, 2012. Disponível em: <http://www.scielo.br/pdf/acb/v27n1/a09v27n1.pdf>. Acesso em: out. 2013.
url http://repositorio.furg.br/handle/1/4070
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositório Institucional da FURG (RI FURG)
instname:Universidade Federal do Rio Grande (FURG)
instacron:FURG
instname_str Universidade Federal do Rio Grande (FURG)
instacron_str FURG
institution FURG
reponame_str Repositório Institucional da FURG (RI FURG)
collection Repositório Institucional da FURG (RI FURG)
repository.name.fl_str_mv Repositório Institucional da FURG (RI FURG) - Universidade Federal do Rio Grande (FURG)
repository.mail.fl_str_mv
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