The role of N-acetylcysteine in the lung remote injury after hepatic ischemia and reperfusion in rabbits
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da FURG (RI FURG) |
Texto Completo: | http://repositorio.furg.br/handle/1/4070 |
Resumo: | PURPOSE: To study the lesions in the lung of rabbits caused by ischemia/reperfusion hepatic (I/R) after the use of N-acetyl-cysteine (NAC). METHODS: Twenty-four rabbits distributed in two groups: control group GI (n = 12) 5% glucose solution and experiment group GII (n = 12) NAC. The animals were pre-anesthetized with 1% acepromazine maleate and anesthetized with ketamine 10% and 2% xylazine intramuscularly. The GI and GII were given glucose solution intravenously or NAC 15min before occlusion of the hepatic pedicle (30 min). After the period of reperfusion of 24h (n = 6) or 48h (n = 6), liver and lung samples were collected for histology and immunohistochemistry to assess the impairment of cell. RESULTS: The animals of GII and GII-24h-48h showed parenchyma liver close to normal, when using NAC. The GII and GII-24h-48h showed lower thickness of alveolar cells that GI and GI-24h-48h. The expression of caspase 3 in lung cells GII presented smaller value compared to the GI group. CONCLUSION: N-acetyl-cysteine administered 15min prior to the injury ischemia/reperfusion had a significant protective role by minimizing lung injury and apoptotic morphology in the period observed. |
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Castro, Angela Pötter deCastro Junior, Miguel Angelo Martins deLauz, SusiFacin, EmilioSimões, Manuel de JesusFagundes, Djalma José2013-10-19T00:47:22Z2013-10-19T00:47:22Z2012CASTRO, Angela Potter de et al. he role of N-acetylcysteine in the lung remote injury after hepatic ischemia and reperfusion in rabbits. Acta Cirúrgica Brasileira, v. 27, n. 1, p. 49-55, 2012. Disponível em: <http://www.scielo.br/pdf/acb/v27n1/a09v27n1.pdf>. Acesso em: out. 2013.http://repositorio.furg.br/handle/1/4070PURPOSE: To study the lesions in the lung of rabbits caused by ischemia/reperfusion hepatic (I/R) after the use of N-acetyl-cysteine (NAC). METHODS: Twenty-four rabbits distributed in two groups: control group GI (n = 12) 5% glucose solution and experiment group GII (n = 12) NAC. The animals were pre-anesthetized with 1% acepromazine maleate and anesthetized with ketamine 10% and 2% xylazine intramuscularly. The GI and GII were given glucose solution intravenously or NAC 15min before occlusion of the hepatic pedicle (30 min). After the period of reperfusion of 24h (n = 6) or 48h (n = 6), liver and lung samples were collected for histology and immunohistochemistry to assess the impairment of cell. RESULTS: The animals of GII and GII-24h-48h showed parenchyma liver close to normal, when using NAC. The GII and GII-24h-48h showed lower thickness of alveolar cells that GI and GI-24h-48h. The expression of caspase 3 in lung cells GII presented smaller value compared to the GI group. CONCLUSION: N-acetyl-cysteine administered 15min prior to the injury ischemia/reperfusion had a significant protective role by minimizing lung injury and apoptotic morphology in the period observed.OBJETIVO: Estudar as lesões no fígado e no pulmão de coelhos, provocadas pela isquemia/reperfusão hepática (I/R) moduladas pelo uso da N-acetil-cisteína (NAC). MÉTODOS: Vinte e quatro coelhos distribuídos em dois grupos: Grupo controle GI (n=12) solução de glicose 5% e Grupo experimento GII (n=12) NAC. Os animais foram pré-anestesiados com maleato de acepromazina 1% e anestesiados com cloridrato de quetamina 10% e xilazina 2% via intramuscular. Os grupos GI e GII receberam solução glicosada ou NAC respectivamente via endovenosa 15min antes da oclusão do pedículo hepático (30 min). Após iniciou-se o período de reperfusão por 24h (n=6) ou 48h (n=6), terminada a reperfusão, amostras do fígado e pulmão foram coletadas para a histologia e imunoistoquímica para avaliar o comprometimento celular. RESULTADOS: Os animais do grupo GII-24h e GII-48h apresentaram arquitetura do parênquima hepático próximo do normal, quando se utilizou NAC. Os grupos GII-24h e GII-48h apresentaram menor espessura das células alveolares que os grupos GI-24h e GI-48h. A expressão da caspase 3 nas células pulmonares do grupo GII, apresentou valor menor comparada ao grupo GI. CONCLUSÃO: A N-acetil-cisteína administrada 15min prévios a lesão da isquemia/reperfusão teve um papel protetor significativo minimizando as lesões morfológicas e apoptóticas pulmonares nos períodos observados.engAcetylcysteineLungReperfusionIschemiaLiverRabbitsAcetilcisteínaPulmãoReperfusãoIsquemiaFígadoCoelhosThe role of N-acetylcysteine in the lung remote injury after hepatic ischemia and reperfusion in rabbitsO papel da N-acetil-cisteína nas lesões pulmonares provocadas à distância pela isquemia e reperfusão hepática em coelhos.info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FURG (RI FURG)instname:Universidade Federal do Rio Grande (FURG)instacron:FURGORIGINALThe role of N-acetylcysteine in the lung remote injury after hepatic ischemia and reperfusion in rabbits.pdfThe role of N-acetylcysteine in the lung remote injury after hepatic ischemia and reperfusion in rabbits.pdfapplication/pdf2216958https://repositorio.furg.br/bitstream/1/4070/1/The%20role%20of%20N-acetylcysteine%20in%20the%20lung%20remote%20injury%20after%20hepatic%20ischemia%20and%20reperfusion%20in%20rabbits.pdf9425abb32b0eb73912dab2ac8dfffde9MD51open accessLICENSElicense.txtlicense.txttext/plain; charset=utf-81678https://repositorio.furg.br/bitstream/1/4070/2/license.txtd3be63d3b3eee02729709361dac69efeMD52open access1/40702014-09-04 12:04:08.138open accessoai:repositorio.furg.br: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ório InstitucionalPUBhttps://repositorio.furg.br/oai/request || http://200.19.254.174/oai/requestopendoar:2014-09-04T15:04:08Repositório Institucional da FURG (RI FURG) - Universidade Federal do Rio Grande (FURG)false |
dc.title.pt_BR.fl_str_mv |
The role of N-acetylcysteine in the lung remote injury after hepatic ischemia and reperfusion in rabbits |
dc.title.alternative.pt_BR.fl_str_mv |
O papel da N-acetil-cisteína nas lesões pulmonares provocadas à distância pela isquemia e reperfusão hepática em coelhos. |
title |
The role of N-acetylcysteine in the lung remote injury after hepatic ischemia and reperfusion in rabbits |
spellingShingle |
The role of N-acetylcysteine in the lung remote injury after hepatic ischemia and reperfusion in rabbits Castro, Angela Pötter de Acetylcysteine Lung Reperfusion Ischemia Liver Rabbits Acetilcisteína Pulmão Reperfusão Isquemia Fígado Coelhos |
title_short |
The role of N-acetylcysteine in the lung remote injury after hepatic ischemia and reperfusion in rabbits |
title_full |
The role of N-acetylcysteine in the lung remote injury after hepatic ischemia and reperfusion in rabbits |
title_fullStr |
The role of N-acetylcysteine in the lung remote injury after hepatic ischemia and reperfusion in rabbits |
title_full_unstemmed |
The role of N-acetylcysteine in the lung remote injury after hepatic ischemia and reperfusion in rabbits |
title_sort |
The role of N-acetylcysteine in the lung remote injury after hepatic ischemia and reperfusion in rabbits |
author |
Castro, Angela Pötter de |
author_facet |
Castro, Angela Pötter de Castro Junior, Miguel Angelo Martins de Lauz, Susi Facin, Emilio Simões, Manuel de Jesus Fagundes, Djalma José |
author_role |
author |
author2 |
Castro Junior, Miguel Angelo Martins de Lauz, Susi Facin, Emilio Simões, Manuel de Jesus Fagundes, Djalma José |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Castro, Angela Pötter de Castro Junior, Miguel Angelo Martins de Lauz, Susi Facin, Emilio Simões, Manuel de Jesus Fagundes, Djalma José |
dc.subject.por.fl_str_mv |
Acetylcysteine Lung Reperfusion Ischemia Liver Rabbits Acetilcisteína Pulmão Reperfusão Isquemia Fígado Coelhos |
topic |
Acetylcysteine Lung Reperfusion Ischemia Liver Rabbits Acetilcisteína Pulmão Reperfusão Isquemia Fígado Coelhos |
description |
PURPOSE: To study the lesions in the lung of rabbits caused by ischemia/reperfusion hepatic (I/R) after the use of N-acetyl-cysteine (NAC). METHODS: Twenty-four rabbits distributed in two groups: control group GI (n = 12) 5% glucose solution and experiment group GII (n = 12) NAC. The animals were pre-anesthetized with 1% acepromazine maleate and anesthetized with ketamine 10% and 2% xylazine intramuscularly. The GI and GII were given glucose solution intravenously or NAC 15min before occlusion of the hepatic pedicle (30 min). After the period of reperfusion of 24h (n = 6) or 48h (n = 6), liver and lung samples were collected for histology and immunohistochemistry to assess the impairment of cell. RESULTS: The animals of GII and GII-24h-48h showed parenchyma liver close to normal, when using NAC. The GII and GII-24h-48h showed lower thickness of alveolar cells that GI and GI-24h-48h. The expression of caspase 3 in lung cells GII presented smaller value compared to the GI group. CONCLUSION: N-acetyl-cysteine administered 15min prior to the injury ischemia/reperfusion had a significant protective role by minimizing lung injury and apoptotic morphology in the period observed. |
publishDate |
2012 |
dc.date.issued.fl_str_mv |
2012 |
dc.date.accessioned.fl_str_mv |
2013-10-19T00:47:22Z |
dc.date.available.fl_str_mv |
2013-10-19T00:47:22Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
CASTRO, Angela Potter de et al. he role of N-acetylcysteine in the lung remote injury after hepatic ischemia and reperfusion in rabbits. Acta Cirúrgica Brasileira, v. 27, n. 1, p. 49-55, 2012. Disponível em: <http://www.scielo.br/pdf/acb/v27n1/a09v27n1.pdf>. Acesso em: out. 2013. |
dc.identifier.uri.fl_str_mv |
http://repositorio.furg.br/handle/1/4070 |
identifier_str_mv |
CASTRO, Angela Potter de et al. he role of N-acetylcysteine in the lung remote injury after hepatic ischemia and reperfusion in rabbits. Acta Cirúrgica Brasileira, v. 27, n. 1, p. 49-55, 2012. Disponível em: <http://www.scielo.br/pdf/acb/v27n1/a09v27n1.pdf>. Acesso em: out. 2013. |
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http://repositorio.furg.br/handle/1/4070 |
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eng |
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eng |
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reponame:Repositório Institucional da FURG (RI FURG) instname:Universidade Federal do Rio Grande (FURG) instacron:FURG |
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Repositório Institucional da FURG (RI FURG) |
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