The role of n-acetyl-cysteine in the lung remote injury after hepatic ischemia and reperfusion in rabbits

Detalhes bibliográficos
Autor(a) principal: Castro, Angela Pötter de
Data de Publicação: 2012
Outros Autores: Castro Junior, Miguel Angelo Martins de, Lauz, Suzi, Facin, Emilio, Simões, Manuel de Jesus, Fagundes, Djalma José
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FURG (RI FURG)
Texto Completo: http://repositorio.furg.br/handle/1/3340
Resumo: PURPOSE: To study the lesions in the lung of rabbits caused by ischemia/reperfusion hepatic (I/R) after the use of N-acetyl-cysteine(NAC). METHODS: Twenty-four rabbits distributed in two groups: control group GI (n = 12) 5% glucose solution and experiment group GII (n = 12) NAC. The animals were pre-anesthetized with 1% acepromazine maleate and anesthetized with ketamine 10% and 2% xylazine intramuscularly. The GI and GII were given glucose solution intravenously or NAC 15min before occlusion of the hepatic pedicle (30 min). After the period of reperfusion of 24h (n = 6) or 48h (n = 6), liver and lung samples were collected for histology and immunohistochemistry to assess the impairment of cell. RESULTS: The animals of GII and GII-24h-48h showed parenchyma liver close to normal, when using NAC. The GII and GII-24h-48h showed lower thickness of alveolar cells that GI and GI-24h-48h. The expression of caspase 3 in lung cells GII presented smaller value compared to the GI group. CONCLUSION: N-acetyl-cysteine administered 15min prior to the injury ischemia/reperfusion had a significant protective role by minimizing lung injury and apoptotic morphology in the period observed.
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spelling Castro, Angela Pötter deCastro Junior, Miguel Angelo Martins deLauz, SuziFacin, EmilioSimões, Manuel de JesusFagundes, Djalma José2013-04-29T17:25:21Z2013-04-29T17:25:21Z2012CASTRO, Angela Pötter de et al. The role of n-acetyl-cysteine in the lung remote injury after hepatic ischemia and reperfusion in rabbits. Acta Cirúrgica Brasileira, v. 27, n. 1, p. 49-55, 2012. Disponível em: <http://www.scielo.br/pdf/acb/v27n1/a09v27n1.pdf>. Acesso em: 29 abr. 2013.1678-2674http://repositorio.furg.br/handle/1/3340PURPOSE: To study the lesions in the lung of rabbits caused by ischemia/reperfusion hepatic (I/R) after the use of N-acetyl-cysteine(NAC). METHODS: Twenty-four rabbits distributed in two groups: control group GI (n = 12) 5% glucose solution and experiment group GII (n = 12) NAC. The animals were pre-anesthetized with 1% acepromazine maleate and anesthetized with ketamine 10% and 2% xylazine intramuscularly. The GI and GII were given glucose solution intravenously or NAC 15min before occlusion of the hepatic pedicle (30 min). After the period of reperfusion of 24h (n = 6) or 48h (n = 6), liver and lung samples were collected for histology and immunohistochemistry to assess the impairment of cell. RESULTS: The animals of GII and GII-24h-48h showed parenchyma liver close to normal, when using NAC. The GII and GII-24h-48h showed lower thickness of alveolar cells that GI and GI-24h-48h. The expression of caspase 3 in lung cells GII presented smaller value compared to the GI group. CONCLUSION: N-acetyl-cysteine administered 15min prior to the injury ischemia/reperfusion had a significant protective role by minimizing lung injury and apoptotic morphology in the period observed.OBJETIVO: Estudar as lesões no fígado e no pulmão de coelhos, provocadas pela isquemia/reperfusão hepática (I/R) moduladas pelo uso da N-acetil-cisteína (NAC). MÉTODOS: Vinte e quatro coelhos distribuídos em dois grupos: Grupo controle GI (n=12) solução de glicose 5% e Grupo experimento GII (n=12) NAC. Os animais foram pré-anestesiados com maleato de acepromazina 1% e anestesiados com cloridrato de quetamina 10% e xilazina 2% via intramuscular. Os grupos GI e GII receberam solução glicosada ou NAC respectivamente via endovenosa 15min antes da oclusão do pedículo hepático (30 min). Após iniciou-se o período de reperfusão por 24h (n=6) ou 48h (n=6), terminada a reperfusão, amostras do fígado e pulmão foram coletadas para a histologia e imunoistoquímica para avaliar o comprometimento celular. RESULTADOS: Os animais do grupo GII-24h e GII-48h apresentaram arquitetura do parênquima hepático próximo do normal, quando se utilizou NAC. Os grupos GII-24h e GII-48h apresentaram menor espessura das células alveolares que os grupos GI-24h e GI-48h. A expressão da caspase 3 nas células pulmonares do grupo GII, apresentou valor menor comparada ao grupo GI. CONCLUSÃO:A N-acetil-cisteína administrada 15min prévios a lesão da isquemia/reperfusão teve um papel protetor significativo minimizando as lesões morfológicas e apoptóticas pulmonares nos períodos observados.engAcetylcysteinLungReperfusionIschemiaLiverRabbitsAcetilcisteínaPulmãoReperfusãoIsquemiaFígadoCoelhosThe role of n-acetyl-cysteine in the lung remote injury after hepatic ischemia and reperfusion in rabbitsO papel da N-acetil-cisteína nas lesões pulmonares provocadas à distância pela isquemia e reperfusão hepática em coelhosinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FURG (RI FURG)instname:Universidade Federal do Rio Grande (FURG)instacron:FURGORIGINAL1.The role of N-acetyl-cysteine in the lung remote injury after hepatic ischemia and reperfusion in rabbits..pdf1.The role of N-acetyl-cysteine in the lung remote injury after hepatic ischemia and reperfusion in rabbits..pdfapplication/pdf2216958https://repositorio.furg.br/bitstream/1/3340/1/1.The%20role%20of%20N-acetyl-cysteine%20in%20the%20lung%20remote%20injury%20after%20hepatic%20ischemia%20and%20reperfusion%20in%20rabbits..pdf9425abb32b0eb73912dab2ac8dfffde9MD51open accessLICENSElicense.txtlicense.txttext/plain; charset=utf-81724https://repositorio.furg.br/bitstream/1/3340/2/license.txt5b92b9704b4f13242d70e45ddef35a68MD52open access1/33402013-04-29 16:24:30.41open accessoai:repositorio.furg.br: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Repositório InstitucionalPUBhttps://repositorio.furg.br/oai/request || http://200.19.254.174/oai/requestopendoar:2013-04-29T19:24:30Repositório Institucional da FURG (RI FURG) - Universidade Federal do Rio Grande (FURG)false
dc.title.pt_BR.fl_str_mv The role of n-acetyl-cysteine in the lung remote injury after hepatic ischemia and reperfusion in rabbits
dc.title.alternative.pt_BR.fl_str_mv O papel da N-acetil-cisteína nas lesões pulmonares provocadas à distância pela isquemia e reperfusão hepática em coelhos
title The role of n-acetyl-cysteine in the lung remote injury after hepatic ischemia and reperfusion in rabbits
spellingShingle The role of n-acetyl-cysteine in the lung remote injury after hepatic ischemia and reperfusion in rabbits
Castro, Angela Pötter de
Acetylcystein
Lung
Reperfusion
Ischemia
Liver
Rabbits
Acetilcisteína
Pulmão
Reperfusão
Isquemia
Fígado
Coelhos
title_short The role of n-acetyl-cysteine in the lung remote injury after hepatic ischemia and reperfusion in rabbits
title_full The role of n-acetyl-cysteine in the lung remote injury after hepatic ischemia and reperfusion in rabbits
title_fullStr The role of n-acetyl-cysteine in the lung remote injury after hepatic ischemia and reperfusion in rabbits
title_full_unstemmed The role of n-acetyl-cysteine in the lung remote injury after hepatic ischemia and reperfusion in rabbits
title_sort The role of n-acetyl-cysteine in the lung remote injury after hepatic ischemia and reperfusion in rabbits
author Castro, Angela Pötter de
author_facet Castro, Angela Pötter de
Castro Junior, Miguel Angelo Martins de
Lauz, Suzi
Facin, Emilio
Simões, Manuel de Jesus
Fagundes, Djalma José
author_role author
author2 Castro Junior, Miguel Angelo Martins de
Lauz, Suzi
Facin, Emilio
Simões, Manuel de Jesus
Fagundes, Djalma José
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Castro, Angela Pötter de
Castro Junior, Miguel Angelo Martins de
Lauz, Suzi
Facin, Emilio
Simões, Manuel de Jesus
Fagundes, Djalma José
dc.subject.por.fl_str_mv Acetylcystein
Lung
Reperfusion
Ischemia
Liver
Rabbits
Acetilcisteína
Pulmão
Reperfusão
Isquemia
Fígado
Coelhos
topic Acetylcystein
Lung
Reperfusion
Ischemia
Liver
Rabbits
Acetilcisteína
Pulmão
Reperfusão
Isquemia
Fígado
Coelhos
description PURPOSE: To study the lesions in the lung of rabbits caused by ischemia/reperfusion hepatic (I/R) after the use of N-acetyl-cysteine(NAC). METHODS: Twenty-four rabbits distributed in two groups: control group GI (n = 12) 5% glucose solution and experiment group GII (n = 12) NAC. The animals were pre-anesthetized with 1% acepromazine maleate and anesthetized with ketamine 10% and 2% xylazine intramuscularly. The GI and GII were given glucose solution intravenously or NAC 15min before occlusion of the hepatic pedicle (30 min). After the period of reperfusion of 24h (n = 6) or 48h (n = 6), liver and lung samples were collected for histology and immunohistochemistry to assess the impairment of cell. RESULTS: The animals of GII and GII-24h-48h showed parenchyma liver close to normal, when using NAC. The GII and GII-24h-48h showed lower thickness of alveolar cells that GI and GI-24h-48h. The expression of caspase 3 in lung cells GII presented smaller value compared to the GI group. CONCLUSION: N-acetyl-cysteine administered 15min prior to the injury ischemia/reperfusion had a significant protective role by minimizing lung injury and apoptotic morphology in the period observed.
publishDate 2012
dc.date.issued.fl_str_mv 2012
dc.date.accessioned.fl_str_mv 2013-04-29T17:25:21Z
dc.date.available.fl_str_mv 2013-04-29T17:25:21Z
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dc.identifier.citation.fl_str_mv CASTRO, Angela Pötter de et al. The role of n-acetyl-cysteine in the lung remote injury after hepatic ischemia and reperfusion in rabbits. Acta Cirúrgica Brasileira, v. 27, n. 1, p. 49-55, 2012. Disponível em: <http://www.scielo.br/pdf/acb/v27n1/a09v27n1.pdf>. Acesso em: 29 abr. 2013.
dc.identifier.uri.fl_str_mv http://repositorio.furg.br/handle/1/3340
dc.identifier.issn.none.fl_str_mv 1678-2674
identifier_str_mv CASTRO, Angela Pötter de et al. The role of n-acetyl-cysteine in the lung remote injury after hepatic ischemia and reperfusion in rabbits. Acta Cirúrgica Brasileira, v. 27, n. 1, p. 49-55, 2012. Disponível em: <http://www.scielo.br/pdf/acb/v27n1/a09v27n1.pdf>. Acesso em: 29 abr. 2013.
1678-2674
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