The Effect of Combined Therapy with L-NAME and Vasoactive Amines on Acute Endotoxemia in Rats.

Detalhes bibliográficos
Autor(a) principal: Batista, Cristiano Corrêa
Data de Publicação: 2003
Outros Autores: Benites, João Alberto Castro, Baisch, Ana Luiza Muccillo
Tipo de documento: Artigo
Idioma: por
Título da fonte: Repositório Institucional da FURG (RI FURG)
Texto Completo: http://repositorio.furg.br/handle/1/151
Resumo: Introduction: Sepsis and its potentially lethal complication, septic shock, are the most frequent causes of mortality, especially, in intensive care units. Cardiovascular changes are often don’t respond to conventional therapy, also the mechanisms by which sepsis leads to hemodynamic disturbance are not fully understood. A common cause of septic shock is gram negative bacterial infection. Nitric Oxide is a key mediator of hypotension, peripheral vasodilatation and vascular hyporeactivity to vasoconstrictor agents in endotoxemia. Objective: The aim of the present study is to verify the hemodynamic and biochemical responses to therapy with dopamine and dobutamine with or without a non-selective inhibitor of Nitric Oxide Synthase (NOS) in rats with shock-like syndrome. Hypothesis: We hypothesized that the inhibition of nitric oxide synthesis along with the intravenous infusion of vasoactives amines such as dopamine and dobutamine would reverse the hemodynamic status, optimizing heart performance without resulting in significant damage to platelet, renal and hepatical functions. Materials and Methods: An experimental model of endotoxic shock was induced in rats by means of intravenous infusion of bolus of lipopolyssaccharide derived from Escherichia coli ( 5 mg/kg ). We evaluated mean arterial blood pressure, heart rate, respiratory rate and urine output. Biochemical data such as platelet count, hepatic enzymes, urea and creatinine were also analysed. The treatment was carried out with the intravenous infusion of dopamine and dobutamine, both in doses of 10 μg/kg/min, through the use of an infusion pump with or without the intravenous bolus infusions of NG-nitro-L-arginine methyl ester ( 100 mg/kg ). Results: The results showed that the use of vasoactive amines dopamine and dobutamine, along with the infusion of bolus of NG-nitro-L-arginine methyl ester, improved the problem of hypotension caused by lipopolyssaccharide. It did not result in heart rate reduction, change in respiratory responses, or an increase in hepatic and renal disfunction. Conclusions: The inhibition of nitric oxide through by non-selective nitric oxide synthase pathways along with the infusion of dopamine and dobutamine can be a useful therapy in the treatment of septic shock.
id FURG_c587cb6bba38a00a3c7ec3a7b9b79703
oai_identifier_str oai:repositorio.furg.br:1/151
network_acronym_str FURG
network_name_str Repositório Institucional da FURG (RI FURG)
repository_id_str
spelling The Effect of Combined Therapy with L-NAME and Vasoactive Amines on Acute Endotoxemia in Rats.Shock-likeSyndromeNitric oxideL-NAMERatsVasoactive animesIntroduction: Sepsis and its potentially lethal complication, septic shock, are the most frequent causes of mortality, especially, in intensive care units. Cardiovascular changes are often don’t respond to conventional therapy, also the mechanisms by which sepsis leads to hemodynamic disturbance are not fully understood. A common cause of septic shock is gram negative bacterial infection. Nitric Oxide is a key mediator of hypotension, peripheral vasodilatation and vascular hyporeactivity to vasoconstrictor agents in endotoxemia. Objective: The aim of the present study is to verify the hemodynamic and biochemical responses to therapy with dopamine and dobutamine with or without a non-selective inhibitor of Nitric Oxide Synthase (NOS) in rats with shock-like syndrome. Hypothesis: We hypothesized that the inhibition of nitric oxide synthesis along with the intravenous infusion of vasoactives amines such as dopamine and dobutamine would reverse the hemodynamic status, optimizing heart performance without resulting in significant damage to platelet, renal and hepatical functions. Materials and Methods: An experimental model of endotoxic shock was induced in rats by means of intravenous infusion of bolus of lipopolyssaccharide derived from Escherichia coli ( 5 mg/kg ). We evaluated mean arterial blood pressure, heart rate, respiratory rate and urine output. Biochemical data such as platelet count, hepatic enzymes, urea and creatinine were also analysed. The treatment was carried out with the intravenous infusion of dopamine and dobutamine, both in doses of 10 μg/kg/min, through the use of an infusion pump with or without the intravenous bolus infusions of NG-nitro-L-arginine methyl ester ( 100 mg/kg ). Results: The results showed that the use of vasoactive amines dopamine and dobutamine, along with the infusion of bolus of NG-nitro-L-arginine methyl ester, improved the problem of hypotension caused by lipopolyssaccharide. It did not result in heart rate reduction, change in respiratory responses, or an increase in hepatic and renal disfunction. Conclusions: The inhibition of nitric oxide through by non-selective nitric oxide synthase pathways along with the infusion of dopamine and dobutamine can be a useful therapy in the treatment of septic shock.2010-10-13T18:25:23Z2010-10-13T18:25:23Z2003info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfBATISTA, C. C.; BENITES, J. A. C. ; MUCCILLO-BAISCH, A. L. The Effect of Combined Therapy with L-NAME and Vasoactive Amines on Acute Endotoxemia in Rats. Revista Brasileira de Terapia Intensiva, São Paulo, v. 15, n. 2, p. 48-57, abr./jun. 2003.0103-507Xhttp://repositorio.furg.br/handle/1/151porBatista, Cristiano CorrêaBenites, João Alberto CastroBaisch, Ana Luiza Muccilloinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FURG (RI FURG)instname:Universidade Federal do Rio Grande (FURG)instacron:FURG2011-08-04T04:01:00Zoai:repositorio.furg.br:1/151Repositório InstitucionalPUBhttps://repositorio.furg.br/oai/request || http://200.19.254.174/oai/requestopendoar:2011-08-04T04:01Repositório Institucional da FURG (RI FURG) - Universidade Federal do Rio Grande (FURG)false
dc.title.none.fl_str_mv The Effect of Combined Therapy with L-NAME and Vasoactive Amines on Acute Endotoxemia in Rats.
title The Effect of Combined Therapy with L-NAME and Vasoactive Amines on Acute Endotoxemia in Rats.
spellingShingle The Effect of Combined Therapy with L-NAME and Vasoactive Amines on Acute Endotoxemia in Rats.
Batista, Cristiano Corrêa
Shock-like
Syndrome
Nitric oxide
L-NAME
Rats
Vasoactive animes
title_short The Effect of Combined Therapy with L-NAME and Vasoactive Amines on Acute Endotoxemia in Rats.
title_full The Effect of Combined Therapy with L-NAME and Vasoactive Amines on Acute Endotoxemia in Rats.
title_fullStr The Effect of Combined Therapy with L-NAME and Vasoactive Amines on Acute Endotoxemia in Rats.
title_full_unstemmed The Effect of Combined Therapy with L-NAME and Vasoactive Amines on Acute Endotoxemia in Rats.
title_sort The Effect of Combined Therapy with L-NAME and Vasoactive Amines on Acute Endotoxemia in Rats.
author Batista, Cristiano Corrêa
author_facet Batista, Cristiano Corrêa
Benites, João Alberto Castro
Baisch, Ana Luiza Muccillo
author_role author
author2 Benites, João Alberto Castro
Baisch, Ana Luiza Muccillo
author2_role author
author
dc.contributor.author.fl_str_mv Batista, Cristiano Corrêa
Benites, João Alberto Castro
Baisch, Ana Luiza Muccillo
dc.subject.por.fl_str_mv Shock-like
Syndrome
Nitric oxide
L-NAME
Rats
Vasoactive animes
topic Shock-like
Syndrome
Nitric oxide
L-NAME
Rats
Vasoactive animes
description Introduction: Sepsis and its potentially lethal complication, septic shock, are the most frequent causes of mortality, especially, in intensive care units. Cardiovascular changes are often don’t respond to conventional therapy, also the mechanisms by which sepsis leads to hemodynamic disturbance are not fully understood. A common cause of septic shock is gram negative bacterial infection. Nitric Oxide is a key mediator of hypotension, peripheral vasodilatation and vascular hyporeactivity to vasoconstrictor agents in endotoxemia. Objective: The aim of the present study is to verify the hemodynamic and biochemical responses to therapy with dopamine and dobutamine with or without a non-selective inhibitor of Nitric Oxide Synthase (NOS) in rats with shock-like syndrome. Hypothesis: We hypothesized that the inhibition of nitric oxide synthesis along with the intravenous infusion of vasoactives amines such as dopamine and dobutamine would reverse the hemodynamic status, optimizing heart performance without resulting in significant damage to platelet, renal and hepatical functions. Materials and Methods: An experimental model of endotoxic shock was induced in rats by means of intravenous infusion of bolus of lipopolyssaccharide derived from Escherichia coli ( 5 mg/kg ). We evaluated mean arterial blood pressure, heart rate, respiratory rate and urine output. Biochemical data such as platelet count, hepatic enzymes, urea and creatinine were also analysed. The treatment was carried out with the intravenous infusion of dopamine and dobutamine, both in doses of 10 μg/kg/min, through the use of an infusion pump with or without the intravenous bolus infusions of NG-nitro-L-arginine methyl ester ( 100 mg/kg ). Results: The results showed that the use of vasoactive amines dopamine and dobutamine, along with the infusion of bolus of NG-nitro-L-arginine methyl ester, improved the problem of hypotension caused by lipopolyssaccharide. It did not result in heart rate reduction, change in respiratory responses, or an increase in hepatic and renal disfunction. Conclusions: The inhibition of nitric oxide through by non-selective nitric oxide synthase pathways along with the infusion of dopamine and dobutamine can be a useful therapy in the treatment of septic shock.
publishDate 2003
dc.date.none.fl_str_mv 2003
2010-10-13T18:25:23Z
2010-10-13T18:25:23Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv BATISTA, C. C.; BENITES, J. A. C. ; MUCCILLO-BAISCH, A. L. The Effect of Combined Therapy with L-NAME and Vasoactive Amines on Acute Endotoxemia in Rats. Revista Brasileira de Terapia Intensiva, São Paulo, v. 15, n. 2, p. 48-57, abr./jun. 2003.
0103-507X
http://repositorio.furg.br/handle/1/151
identifier_str_mv BATISTA, C. C.; BENITES, J. A. C. ; MUCCILLO-BAISCH, A. L. The Effect of Combined Therapy with L-NAME and Vasoactive Amines on Acute Endotoxemia in Rats. Revista Brasileira de Terapia Intensiva, São Paulo, v. 15, n. 2, p. 48-57, abr./jun. 2003.
0103-507X
url http://repositorio.furg.br/handle/1/151
dc.language.iso.fl_str_mv por
language por
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositório Institucional da FURG (RI FURG)
instname:Universidade Federal do Rio Grande (FURG)
instacron:FURG
instname_str Universidade Federal do Rio Grande (FURG)
instacron_str FURG
institution FURG
reponame_str Repositório Institucional da FURG (RI FURG)
collection Repositório Institucional da FURG (RI FURG)
repository.name.fl_str_mv Repositório Institucional da FURG (RI FURG) - Universidade Federal do Rio Grande (FURG)
repository.mail.fl_str_mv
_version_ 1813187277414727680