Activity of rice bran proteic extracts against Fusarium graminearum
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da FURG (RI FURG) |
Texto Completo: | http://repositorio.furg.br/handle/1/4669 |
Resumo: | The application of natural antifungal substances is motivated by the need for alternatives to existing methods that are not always applicable, efficient, or that do not pose risk to consumers or the environment. Furthermore, studies on the behaviour of toxigenic species in the presence of natural fungicides have enabled their safe application in the food chain. This study aimed to identify the fraction of the rice grain with greater inhibitory activity of amylase and related to its antifungal and antimycotoxigenic potential against Fusarium graminearum CQ 244 biomass. The greatest inhibitory effect was observed in extracts of bran, which inhibited by 90% the fungal amylase activity. The primary fractionation of the rice bran extract was more efficient when ethanolic extracts was precipitated by acetone, resulting in a specific inhibition estimated at 20 μg min-1mg protein1, PF 45 and recovery 61%. The rice bran protein extracts showed fungistatic activity against F. graminearum, with MIC50 of 419 μg ml-1 and 168 mg ml-1 estimated from glucosamine and amylase inhibition, respectively, which cause 63% biomass inhibition and 40% of the nivalenol (NIV) production. |
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Activity of rice bran proteic extracts against Fusarium graminearumRiceFusarium graminearumGlucosamineAmylaseNivalenol (NIV)The application of natural antifungal substances is motivated by the need for alternatives to existing methods that are not always applicable, efficient, or that do not pose risk to consumers or the environment. Furthermore, studies on the behaviour of toxigenic species in the presence of natural fungicides have enabled their safe application in the food chain. This study aimed to identify the fraction of the rice grain with greater inhibitory activity of amylase and related to its antifungal and antimycotoxigenic potential against Fusarium graminearum CQ 244 biomass. The greatest inhibitory effect was observed in extracts of bran, which inhibited by 90% the fungal amylase activity. The primary fractionation of the rice bran extract was more efficient when ethanolic extracts was precipitated by acetone, resulting in a specific inhibition estimated at 20 μg min-1mg protein1, PF 45 and recovery 61%. The rice bran protein extracts showed fungistatic activity against F. graminearum, with MIC50 of 419 μg ml-1 and 168 mg ml-1 estimated from glucosamine and amylase inhibition, respectively, which cause 63% biomass inhibition and 40% of the nivalenol (NIV) production.2014-11-16T00:52:55Z2014-11-16T00:52:55Z2013info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfPAGNUSSATT, Fernanda Arnhold et al.Activity of rice bran proteic extracts against Fusarium graminearum. African Journal of Agricultural Research, v. 8, n. 48, p. 6283-6290, 2013. Disponível em: <http://www.academicjournals.org/article/article1387361989_Fernanda%20et%20al.pdf>. Acesso em: 07 nov. 20141991-637Xhttp://repositorio.furg.br/handle/1/466910.5897/AJAR2013.7358engPagnussatt, Fernanda ArnholdBretanha, Cristiana CostaMeza, Silvia Leticia RiveroBuffon, Jaqueline GardaFurlong, Eliana Badialeinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FURG (RI FURG)instname:Universidade Federal do Rio Grande (FURG)instacron:FURG2014-11-16T00:52:55Zoai:repositorio.furg.br:1/4669Repositório InstitucionalPUBhttps://repositorio.furg.br/oai/request || http://200.19.254.174/oai/requestopendoar:2014-11-16T00:52:55Repositório Institucional da FURG (RI FURG) - Universidade Federal do Rio Grande (FURG)false |
dc.title.none.fl_str_mv |
Activity of rice bran proteic extracts against Fusarium graminearum |
title |
Activity of rice bran proteic extracts against Fusarium graminearum |
spellingShingle |
Activity of rice bran proteic extracts against Fusarium graminearum Pagnussatt, Fernanda Arnhold Rice Fusarium graminearum Glucosamine Amylase Nivalenol (NIV) |
title_short |
Activity of rice bran proteic extracts against Fusarium graminearum |
title_full |
Activity of rice bran proteic extracts against Fusarium graminearum |
title_fullStr |
Activity of rice bran proteic extracts against Fusarium graminearum |
title_full_unstemmed |
Activity of rice bran proteic extracts against Fusarium graminearum |
title_sort |
Activity of rice bran proteic extracts against Fusarium graminearum |
author |
Pagnussatt, Fernanda Arnhold |
author_facet |
Pagnussatt, Fernanda Arnhold Bretanha, Cristiana Costa Meza, Silvia Leticia Rivero Buffon, Jaqueline Garda Furlong, Eliana Badiale |
author_role |
author |
author2 |
Bretanha, Cristiana Costa Meza, Silvia Leticia Rivero Buffon, Jaqueline Garda Furlong, Eliana Badiale |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Pagnussatt, Fernanda Arnhold Bretanha, Cristiana Costa Meza, Silvia Leticia Rivero Buffon, Jaqueline Garda Furlong, Eliana Badiale |
dc.subject.por.fl_str_mv |
Rice Fusarium graminearum Glucosamine Amylase Nivalenol (NIV) |
topic |
Rice Fusarium graminearum Glucosamine Amylase Nivalenol (NIV) |
description |
The application of natural antifungal substances is motivated by the need for alternatives to existing methods that are not always applicable, efficient, or that do not pose risk to consumers or the environment. Furthermore, studies on the behaviour of toxigenic species in the presence of natural fungicides have enabled their safe application in the food chain. This study aimed to identify the fraction of the rice grain with greater inhibitory activity of amylase and related to its antifungal and antimycotoxigenic potential against Fusarium graminearum CQ 244 biomass. The greatest inhibitory effect was observed in extracts of bran, which inhibited by 90% the fungal amylase activity. The primary fractionation of the rice bran extract was more efficient when ethanolic extracts was precipitated by acetone, resulting in a specific inhibition estimated at 20 μg min-1mg protein1, PF 45 and recovery 61%. The rice bran protein extracts showed fungistatic activity against F. graminearum, with MIC50 of 419 μg ml-1 and 168 mg ml-1 estimated from glucosamine and amylase inhibition, respectively, which cause 63% biomass inhibition and 40% of the nivalenol (NIV) production. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013 2014-11-16T00:52:55Z 2014-11-16T00:52:55Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
PAGNUSSATT, Fernanda Arnhold et al.Activity of rice bran proteic extracts against Fusarium graminearum. African Journal of Agricultural Research, v. 8, n. 48, p. 6283-6290, 2013. Disponível em: <http://www.academicjournals.org/article/article1387361989_Fernanda%20et%20al.pdf>. Acesso em: 07 nov. 2014 1991-637X http://repositorio.furg.br/handle/1/4669 10.5897/AJAR2013.7358 |
identifier_str_mv |
PAGNUSSATT, Fernanda Arnhold et al.Activity of rice bran proteic extracts against Fusarium graminearum. African Journal of Agricultural Research, v. 8, n. 48, p. 6283-6290, 2013. Disponível em: <http://www.academicjournals.org/article/article1387361989_Fernanda%20et%20al.pdf>. Acesso em: 07 nov. 2014 1991-637X 10.5897/AJAR2013.7358 |
url |
http://repositorio.furg.br/handle/1/4669 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da FURG (RI FURG) instname:Universidade Federal do Rio Grande (FURG) instacron:FURG |
instname_str |
Universidade Federal do Rio Grande (FURG) |
instacron_str |
FURG |
institution |
FURG |
reponame_str |
Repositório Institucional da FURG (RI FURG) |
collection |
Repositório Institucional da FURG (RI FURG) |
repository.name.fl_str_mv |
Repositório Institucional da FURG (RI FURG) - Universidade Federal do Rio Grande (FURG) |
repository.mail.fl_str_mv |
|
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1807384361784311808 |