Tissue culture optimization for Lallementia royleana L. an important medicinal plant

Detalhes bibliográficos
Autor(a) principal: Mahmood, Tariq
Data de Publicação: 2021
Outros Autores: Arshad, Huma, Akhtar, Wasim, Ilyas, Muhammad, Habib, Tariq, Shinwari, Zabta Khan
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Iheringia. Série Botânica
Texto Completo: https://isb.emnuvens.com.br/iheringia/article/view/889
Resumo: This study was designed to develop an efficient protocol for Lallementia royleana in vitro culturing and to analyze the effect of various hormones on its culturing ability. The indirect organogenesis and its callogenetic ability were evaluated; with the best (100%) results with 1 mg L-1 of benzyl aminopurine (BAP) and 2 mg L-1 of indole acetic acid (IAA) combination. In direct regeneration, leave petioles, nodes and internodes explants were cultured for shooting on various concentrations of BAP and kinetin (KIN). Maximum shooting (80%) was obtained from nodal parts with 1.0 mg L-1 of BAP and 0.5 mg L-1 of KIN. Similarly, the best in vitro rooting (24 roots) was achieved with 1 mg L-1 naphthalene acetic acid (NAA). Lallementia royleana showed higher regeneration through direct regeneration than callogenesis. Significant variability (p = 0.002625) among callus induction, plant parts and hormonal combinations were observed. The node and internode explants showed observable callus responses in combinations of 2, 4-Dichlorophenoxyacetic acid (2,4-D) with NAA, and BAP with IAA.   
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spelling Tissue culture optimization for Lallementia royleana L. an important medicinal plantin vitro culturemicropropagationshootingIn vitro Culturecultura in vitromicropropagaçãotiroThis study was designed to develop an efficient protocol for Lallementia royleana in vitro culturing and to analyze the effect of various hormones on its culturing ability. The indirect organogenesis and its callogenetic ability were evaluated; with the best (100%) results with 1 mg L-1 of benzyl aminopurine (BAP) and 2 mg L-1 of indole acetic acid (IAA) combination. In direct regeneration, leave petioles, nodes and internodes explants were cultured for shooting on various concentrations of BAP and kinetin (KIN). Maximum shooting (80%) was obtained from nodal parts with 1.0 mg L-1 of BAP and 0.5 mg L-1 of KIN. Similarly, the best in vitro rooting (24 roots) was achieved with 1 mg L-1 naphthalene acetic acid (NAA). Lallementia royleana showed higher regeneration through direct regeneration than callogenesis. Significant variability (p = 0.002625) among callus induction, plant parts and hormonal combinations were observed. The node and internode explants showed observable callus responses in combinations of 2, 4-Dichlorophenoxyacetic acid (2,4-D) with NAA, and BAP with IAA.   O estudo foi realizado para desenvolver um protocolo eficiente para a cultura in vitro de Lallementia royleana e para analisar o efeito de vários hormônios em sua capacidade de cultura. Na organogênese indireta, sua capacidade calogenética foi avaliada com os melhores resultados (100%) com 1 mg L-1 de benzil aminopurina (BAP) e 2 mg L-1 e combinação de ácido indolacético (IAA). Na regeneração indireta, explantes de pecíolo de folhas, nós e entrenós foram cultivados para filmagem em várias concentrações de BAP e cinetina (KIN). O valor máximo (80%) foi obtido das partes nodais com 1,0 mg L-1 de BAP e 0,5 mg L-1 de KIN. Da mesma forma, o melhor enraizamento in vitro (24 raízes) foi obtido com 1 mg L-1 de ácido naftaleno acético (NAA). L. royleana apresentou maior regeneração por regeneração direta do que por calogênese. Observou-se variabilidade significativa (p = 0,002625) entre indução de calos, partes de plantas e combinações hormonais. Os explantes de nódulos e internódios apresentaram respostas observáveis de calos em combinações de ácido 2, 4-diclorofenoxiacético (2,4-D) com NAA e BAP com IAA.Iheringia. Série Botânica.Iheringia, Série Botânica.2021-05-25info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://isb.emnuvens.com.br/iheringia/article/view/88910.21826/2446-82312021v76e2021009Iheringia. Série Botânica.; Vol. 76 (2021)Iheringia, Série Botânica.; v. 76 (2021)2446-82310073-4705reponame:Iheringia. Série Botânicainstname:Fundação Zoobotânica do Rio Grande do Sul (FZB/RS)instacron:FZB/RSenghttps://isb.emnuvens.com.br/iheringia/article/view/889/523Copyright (c) 2021 Iheringia. Série Botânica.info:eu-repo/semantics/openAccessMahmood, TariqArshad, HumaAkhtar, WasimIlyas, MuhammadHabib, TariqShinwari, Zabta Khan2021-05-25T19:52:56Zoai:ojs.emnuvens.com.br:article/889Revistahttps://isb.emnuvens.com.br/iheringiaONGhttps://isb.emnuvens.com.br/iheringia/oai||iheringia-bot@fzb.rs.gov.br2446-82310073-4705opendoar:2021-05-25T19:52:56Iheringia. Série Botânica - Fundação Zoobotânica do Rio Grande do Sul (FZB/RS)false
dc.title.none.fl_str_mv Tissue culture optimization for Lallementia royleana L. an important medicinal plant
title Tissue culture optimization for Lallementia royleana L. an important medicinal plant
spellingShingle Tissue culture optimization for Lallementia royleana L. an important medicinal plant
Mahmood, Tariq
in vitro culture
micropropagation
shooting
In vitro Culture
cultura in vitro
micropropagação
tiro
title_short Tissue culture optimization for Lallementia royleana L. an important medicinal plant
title_full Tissue culture optimization for Lallementia royleana L. an important medicinal plant
title_fullStr Tissue culture optimization for Lallementia royleana L. an important medicinal plant
title_full_unstemmed Tissue culture optimization for Lallementia royleana L. an important medicinal plant
title_sort Tissue culture optimization for Lallementia royleana L. an important medicinal plant
author Mahmood, Tariq
author_facet Mahmood, Tariq
Arshad, Huma
Akhtar, Wasim
Ilyas, Muhammad
Habib, Tariq
Shinwari, Zabta Khan
author_role author
author2 Arshad, Huma
Akhtar, Wasim
Ilyas, Muhammad
Habib, Tariq
Shinwari, Zabta Khan
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Mahmood, Tariq
Arshad, Huma
Akhtar, Wasim
Ilyas, Muhammad
Habib, Tariq
Shinwari, Zabta Khan
dc.subject.por.fl_str_mv in vitro culture
micropropagation
shooting
In vitro Culture
cultura in vitro
micropropagação
tiro
topic in vitro culture
micropropagation
shooting
In vitro Culture
cultura in vitro
micropropagação
tiro
description This study was designed to develop an efficient protocol for Lallementia royleana in vitro culturing and to analyze the effect of various hormones on its culturing ability. The indirect organogenesis and its callogenetic ability were evaluated; with the best (100%) results with 1 mg L-1 of benzyl aminopurine (BAP) and 2 mg L-1 of indole acetic acid (IAA) combination. In direct regeneration, leave petioles, nodes and internodes explants were cultured for shooting on various concentrations of BAP and kinetin (KIN). Maximum shooting (80%) was obtained from nodal parts with 1.0 mg L-1 of BAP and 0.5 mg L-1 of KIN. Similarly, the best in vitro rooting (24 roots) was achieved with 1 mg L-1 naphthalene acetic acid (NAA). Lallementia royleana showed higher regeneration through direct regeneration than callogenesis. Significant variability (p = 0.002625) among callus induction, plant parts and hormonal combinations were observed. The node and internode explants showed observable callus responses in combinations of 2, 4-Dichlorophenoxyacetic acid (2,4-D) with NAA, and BAP with IAA.   
publishDate 2021
dc.date.none.fl_str_mv 2021-05-25
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://isb.emnuvens.com.br/iheringia/article/view/889
10.21826/2446-82312021v76e2021009
url https://isb.emnuvens.com.br/iheringia/article/view/889
identifier_str_mv 10.21826/2446-82312021v76e2021009
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://isb.emnuvens.com.br/iheringia/article/view/889/523
dc.rights.driver.fl_str_mv Copyright (c) 2021 Iheringia. Série Botânica.
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2021 Iheringia. Série Botânica.
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Iheringia. Série Botânica.
Iheringia, Série Botânica.
publisher.none.fl_str_mv Iheringia. Série Botânica.
Iheringia, Série Botânica.
dc.source.none.fl_str_mv Iheringia. Série Botânica.; Vol. 76 (2021)
Iheringia, Série Botânica.; v. 76 (2021)
2446-8231
0073-4705
reponame:Iheringia. Série Botânica
instname:Fundação Zoobotânica do Rio Grande do Sul (FZB/RS)
instacron:FZB/RS
instname_str Fundação Zoobotânica do Rio Grande do Sul (FZB/RS)
instacron_str FZB/RS
institution FZB/RS
reponame_str Iheringia. Série Botânica
collection Iheringia. Série Botânica
repository.name.fl_str_mv Iheringia. Série Botânica - Fundação Zoobotânica do Rio Grande do Sul (FZB/RS)
repository.mail.fl_str_mv ||iheringia-bot@fzb.rs.gov.br
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