Genetic transformation with the gfp gene of Colletotrichum gloeosporioides isolates from coffee with blister spot

Detalhes bibliográficos
Autor(a) principal: Armesto,Cecilia
Data de Publicação: 2012
Outros Autores: Maia,Fernanda Gonçalves Martins, Abreu,Mário Sobral de, Figueira,Antonia dos Reis, Silva,Bruno Marques da, Monteiro,Fernando Pereira
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Journal of Microbiology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000300050
Resumo: Blister spot (Colletotrichum gloeosporioides) is now widespread in most coffee producing states of Brazil, becoming a limiting factor for production. The lack of data relating to the reproduction of typical symptoms (light green, oily patches) leaves a gap within the pathosystem, forcing the search for new methodologies for monitoring the disease. Monitoring of genetically modified organisms has proven to be an effective tool in understanding the host x pathogen interactions. Thus, the present study was carried out to evaluate the effectiveness of two systems of genetic transformation in obtaining mutants using the gfp reporter gene. Using the two transformation systems (PEG and electroporation) revealed the efficiency of both, confirmed by fluorescence microscopy and resistance to the antibiotic hygromycin-B, when incorporated into the culture medium. The fungus maintained its cultural and morphological characteristics when compared to wild strains. When inoculated on coffee seedlings, it was found that the pathogenicity of the processed isolates had not changed.
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spelling Genetic transformation with the gfp gene of Colletotrichum gloeosporioides isolates from coffee with blister spotColletotrichum gloeosporioidesFluorescent proteinsPathogenicityBlister spot (Colletotrichum gloeosporioides) is now widespread in most coffee producing states of Brazil, becoming a limiting factor for production. The lack of data relating to the reproduction of typical symptoms (light green, oily patches) leaves a gap within the pathosystem, forcing the search for new methodologies for monitoring the disease. Monitoring of genetically modified organisms has proven to be an effective tool in understanding the host x pathogen interactions. Thus, the present study was carried out to evaluate the effectiveness of two systems of genetic transformation in obtaining mutants using the gfp reporter gene. Using the two transformation systems (PEG and electroporation) revealed the efficiency of both, confirmed by fluorescence microscopy and resistance to the antibiotic hygromycin-B, when incorporated into the culture medium. The fungus maintained its cultural and morphological characteristics when compared to wild strains. When inoculated on coffee seedlings, it was found that the pathogenicity of the processed isolates had not changed.Sociedade Brasileira de Microbiologia2012-09-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000300050Brazilian Journal of Microbiology v.43 n.3 2012reponame:Brazilian Journal of Microbiologyinstname:Sociedade Brasileira de Microbiologia (SBM)instacron:SBM10.1590/S1517-83822012000300050info:eu-repo/semantics/openAccessArmesto,CeciliaMaia,Fernanda Gonçalves MartinsAbreu,Mário Sobral deFigueira,Antonia dos ReisSilva,Bruno Marques daMonteiro,Fernando Pereiraeng2014-10-31T00:00:00Zoai:scielo:S1517-83822012000300050Revistahttps://www.scielo.br/j/bjm/ONGhttps://old.scielo.br/oai/scielo-oai.phpbjm@sbmicrobiologia.org.br||mbmartin@usp.br1678-44051517-8382opendoar:2014-10-31T00:00Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)false
dc.title.none.fl_str_mv Genetic transformation with the gfp gene of Colletotrichum gloeosporioides isolates from coffee with blister spot
title Genetic transformation with the gfp gene of Colletotrichum gloeosporioides isolates from coffee with blister spot
spellingShingle Genetic transformation with the gfp gene of Colletotrichum gloeosporioides isolates from coffee with blister spot
Armesto,Cecilia
Colletotrichum gloeosporioides
Fluorescent proteins
Pathogenicity
title_short Genetic transformation with the gfp gene of Colletotrichum gloeosporioides isolates from coffee with blister spot
title_full Genetic transformation with the gfp gene of Colletotrichum gloeosporioides isolates from coffee with blister spot
title_fullStr Genetic transformation with the gfp gene of Colletotrichum gloeosporioides isolates from coffee with blister spot
title_full_unstemmed Genetic transformation with the gfp gene of Colletotrichum gloeosporioides isolates from coffee with blister spot
title_sort Genetic transformation with the gfp gene of Colletotrichum gloeosporioides isolates from coffee with blister spot
author Armesto,Cecilia
author_facet Armesto,Cecilia
Maia,Fernanda Gonçalves Martins
Abreu,Mário Sobral de
Figueira,Antonia dos Reis
Silva,Bruno Marques da
Monteiro,Fernando Pereira
author_role author
author2 Maia,Fernanda Gonçalves Martins
Abreu,Mário Sobral de
Figueira,Antonia dos Reis
Silva,Bruno Marques da
Monteiro,Fernando Pereira
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Armesto,Cecilia
Maia,Fernanda Gonçalves Martins
Abreu,Mário Sobral de
Figueira,Antonia dos Reis
Silva,Bruno Marques da
Monteiro,Fernando Pereira
dc.subject.por.fl_str_mv Colletotrichum gloeosporioides
Fluorescent proteins
Pathogenicity
topic Colletotrichum gloeosporioides
Fluorescent proteins
Pathogenicity
description Blister spot (Colletotrichum gloeosporioides) is now widespread in most coffee producing states of Brazil, becoming a limiting factor for production. The lack of data relating to the reproduction of typical symptoms (light green, oily patches) leaves a gap within the pathosystem, forcing the search for new methodologies for monitoring the disease. Monitoring of genetically modified organisms has proven to be an effective tool in understanding the host x pathogen interactions. Thus, the present study was carried out to evaluate the effectiveness of two systems of genetic transformation in obtaining mutants using the gfp reporter gene. Using the two transformation systems (PEG and electroporation) revealed the efficiency of both, confirmed by fluorescence microscopy and resistance to the antibiotic hygromycin-B, when incorporated into the culture medium. The fungus maintained its cultural and morphological characteristics when compared to wild strains. When inoculated on coffee seedlings, it was found that the pathogenicity of the processed isolates had not changed.
publishDate 2012
dc.date.none.fl_str_mv 2012-09-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000300050
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000300050
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1517-83822012000300050
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Microbiologia
publisher.none.fl_str_mv Sociedade Brasileira de Microbiologia
dc.source.none.fl_str_mv Brazilian Journal of Microbiology v.43 n.3 2012
reponame:Brazilian Journal of Microbiology
instname:Sociedade Brasileira de Microbiologia (SBM)
instacron:SBM
instname_str Sociedade Brasileira de Microbiologia (SBM)
instacron_str SBM
institution SBM
reponame_str Brazilian Journal of Microbiology
collection Brazilian Journal of Microbiology
repository.name.fl_str_mv Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)
repository.mail.fl_str_mv bjm@sbmicrobiologia.org.br||mbmartin@usp.br
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