Prevalence of human astrovirus genotypes associated with acute gastroenteritis among children in Belém, Brazil

• Autor(a) principal: Gabbay, Yvone Benchimol
• Data de Publicação: 2007
• Outros Autores: Linhares, Alexandre da Costa, Pepino, Elielma Lira Cavalcante, Nakamura, Liliany Satiko, Oliveira, Darleise de Souza, Silva, Luciana Damascena da, Mascarenhas, Joana D'Arc Pereira, Oliveira, Consuelo Silva de, Monteiro, Talita Antônia Furtado, Leite, José Paulo Gagliardi
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Digital do Instituto Evandro Chagas (Patuá)
• Texto Completo: https://patua.iec.gov.br/handle/iec/3257
• topic: Infecções por Astroviridae / epidemiologia; Mamastrovirus; Vacinas contra Rotavirus; Variação Genética; Reação em Cadeia da Polimerase; Diarreia / virologia; Gastroenterite

Worldwide human astroviruses (HAstV) have increasingly been recognized as causative agents of viral gastroenteritis, mainly in infants and young children. The aim of this study was to assess the epidemiology and genotype diversity of HAstVs detected in children who participated in a trial in Belem, Brazil with the rhesus human reassortant rotavirus vaccine tetravalent (RRV-TV). From April/1990 to August/1992, 624 diarrheic stool samples were tested by enzyme immunoassay (EIA) for HAstV, with a positive rate of 4.0 per cent. Reverse transcription-polymerase chain reaction (RT-PCR) was done in 129 samples (25 positive and 104 with twice the optical density (OD) value of negative control by EIA) being 33 positive. The overall positivity yielded by both methods was 5.4 per cent (34/624). Genotyping of the 33 positive samples was done by type-specific RT-PCR and confirmed by sequence analysis. Phylogenetic analysis was performed using a 348-bp fragment of the ORF2 region of the capsid gene. HAstV-1 was the most prevalent, accounting for 45.5 per cent of the isolates, followed by HAstV-2 (27.3 per cent), HAstV-3 (12.1 per cent), HAstV-4 (12.1 per cent), and HAstV-6 (3.0 per cent). The monthly distribution showed that HAstV-1 was predominant in the first year of study (May/1990 to May/1991) with highest prevalence in January/1991. HAstV-2 was predominant from July to November/1991 and HAstV-4 from September to October/1990. At 24 months of age, 30.6 per cent of children had been infected by HAstV. The clinical symptoms registered during HAstV associated-diarrhea were usually mild. These data highlight the circulation of the different HAstV genotypes in Belem during the study period.