Astrovírus infection in hospitalized children: molecular, clinical and epidemiological features
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Digital do Instituto Evandro Chagas (Patuá) |
Texto Completo: | https://patua.iec.gov.br/handle/iec/2880 |
Resumo: | Background: Astrovirus (HAstV) is a common viral pathogen that causes gastroenteritis worldwide. It is classified into eight classical human types (HAstV-1/8) and seven other less prevalent types, described as HAstV VA1, VA2, VA3, VA4, MLB-1, MLB-2 and MLB-3. During outbreaks, the elderly and children are the most affected, and the spread of the virus is associated with person-to-person contact, food ingestion and contaminated water. Objectives: The aim of the present study was to investigate the prevalence of infection and genetic diversity of HAstV strains. Samples were collected from children with acute gastroenteritis admitted to a large pediatric hospital during a surveillance period of three years (2008–2011) in Belém city, Pará State, Amazon Region, Northern Brazil. Study design: Screening and genotyping tests were conducted using RT-PCR to detect the classical and non-classical HAstV types using specific primers. A semi-nested RT-PCR protocol was developed to improve viral detection in samples with a low viral load. Results: The overall positivity observed in this study was 3.9% (19/483). The age distribution showed a high prevalence of positive cases in children under one year old (5.3%). We found vomiting associated with 75% of the positive cases, fever with 82.3%, and dehydration with 76.9%. Most patients with positive cases demonstrated two to five days of diarrhea, two to three episodes of vomiting during hospitalization, and three bowel movements per day. Co-infection with HAstV and norovirus was observed in three cases (15.8%), and no pattern of seasonality or any relationship between the HAstV positivity rate and climate variables was observed. Eighteen positive samples (94.7%–18/19) were genotyped based on the ORF 2 region, and the greatest prevalence was of HAstV-1a (66.6%–12/18), followed by HAstV-2 (22.2%–4/18, comprising two type-2b and two type-2c genotypes), HAstV-3c (5.6%–1/18) and HAstV-4c (5.6%–1/18). No non-classical types were detected in the clinical samples analyzed. Conclusions: The present study showed that although HAstV infections occur at low frequency, they are involved in severe pediatric cases of acute gastroenteritis presenting with a high diversity of strains, including the lineages 3c and 4c, which were never before detected in Brazil. |
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Siqueira, Jones Anderson MonteiroOliveira, Darleise de SouzaCarvalho, Thaís Cristina Nascimento dePortal, Thayara MoraisJustino, Maria Cleonice AguiarSilva, Luciana Damascena daResque, Hugo ReisGabbay, Yvone Benchimol2017-11-24T16:28:06Z2017-11-24T16:28:06Z2017SIQUEIRA, Jones Anderson Monteiro et al. Astrovírus infection in hospitalized children: molecular, clinical and epidemiological features. Journal of Clinical Virology, v. 94, p. 79-85, Sept. 2017.1386-6532https://patua.iec.gov.br/handle/iec/288010.1016/j.jcv.2017.07.014Background: Astrovirus (HAstV) is a common viral pathogen that causes gastroenteritis worldwide. It is classified into eight classical human types (HAstV-1/8) and seven other less prevalent types, described as HAstV VA1, VA2, VA3, VA4, MLB-1, MLB-2 and MLB-3. During outbreaks, the elderly and children are the most affected, and the spread of the virus is associated with person-to-person contact, food ingestion and contaminated water. Objectives: The aim of the present study was to investigate the prevalence of infection and genetic diversity of HAstV strains. Samples were collected from children with acute gastroenteritis admitted to a large pediatric hospital during a surveillance period of three years (2008–2011) in Belém city, Pará State, Amazon Region, Northern Brazil. Study design: Screening and genotyping tests were conducted using RT-PCR to detect the classical and non-classical HAstV types using specific primers. A semi-nested RT-PCR protocol was developed to improve viral detection in samples with a low viral load. Results: The overall positivity observed in this study was 3.9% (19/483). The age distribution showed a high prevalence of positive cases in children under one year old (5.3%). We found vomiting associated with 75% of the positive cases, fever with 82.3%, and dehydration with 76.9%. Most patients with positive cases demonstrated two to five days of diarrhea, two to three episodes of vomiting during hospitalization, and three bowel movements per day. Co-infection with HAstV and norovirus was observed in three cases (15.8%), and no pattern of seasonality or any relationship between the HAstV positivity rate and climate variables was observed. Eighteen positive samples (94.7%–18/19) were genotyped based on the ORF 2 region, and the greatest prevalence was of HAstV-1a (66.6%–12/18), followed by HAstV-2 (22.2%–4/18, comprising two type-2b and two type-2c genotypes), HAstV-3c (5.6%–1/18) and HAstV-4c (5.6%–1/18). No non-classical types were detected in the clinical samples analyzed. Conclusions: The present study showed that although HAstV infections occur at low frequency, they are involved in severe pediatric cases of acute gastroenteritis presenting with a high diversity of strains, including the lineages 3c and 4c, which were never before detected in Brazil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Programa de Pós-Graduação em Virologia. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Programa de Pós-Graduação em Virologia. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.engElsevierAstrovírus infection in hospitalized children: molecular, clinical and epidemiological featuresinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article20082011Astroviridae / patogenicidadeGastroenteriteMamastrovirus / patogenicidadeDiarreiaRegião Amazônica (BR)Belém (PA)info:eu-repo/semantics/embargoedAccessreponame:Repositório Digital do Instituto Evandro Chagas (Patuá)instname:Instituto Evandro Chagas (IEC)instacron:IECLICENSElicense.txtlicense.txttext/plain; charset=utf-871https://patua.iec.gov.br/bitstreams/74cc8ddd-cf0d-4da4-be9d-f721af9ec995/download52f1732ea66fbd1123abe39f5373b797MD52ORIGINALAstrovírus infection in hospitalized children: molecular, clinical and epidemiological features.pdfAstrovírus infection in hospitalized children: molecular, clinical and epidemiological features.pdfapplication/pdf551083https://patua.iec.gov.br/bitstreams/28752f57-c1de-4c69-b2ed-b233770cd81b/downloadc9a9c128e29cac82a5d7fdf3f4e6da73MD56TEXTAstrovírus infection in hospitalized children: molecular, clinical and epidemiological features.pdf.txtAstrovírus infection in hospitalized children: molecular, clinical and epidemiological features.pdf.txtExtracted texttext/plain2https://patua.iec.gov.br/bitstreams/bc21629d-f422-4204-b390-9cc2bc92478b/downloade1c06d85ae7b8b032bef47e42e4c08f9MD57THUMBNAILAstrovírus infection in hospitalized children: molecular, clinical and epidemiological features.pdf.jpgAstrovírus infection in hospitalized children: molecular, clinical and epidemiological features.pdf.jpgGenerated Thumbnailimage/jpeg3095https://patua.iec.gov.br/bitstreams/a883f210-8ac5-45a9-a2ad-5da365aa32e3/download71859d578212107f7f8c49a4ce09d9eeMD58iec/28802022-10-20 21:11:42.007oai:patua.iec.gov.br:iec/2880https://patua.iec.gov.brRepositório InstitucionalPUBhttps://patua.iec.gov.br/oai/requestclariceneta@iec.gov.br || Biblioteca@iec.gov.bropendoar:2022-10-20T21:11:42Repositório Digital do Instituto Evandro Chagas (Patuá) - Instituto Evandro Chagas (IEC)falseVG9kb3Mgb3MgZG9jdW1lbnRvcyBkZXNzYSBjb2xlw6fDo28gc2VndWVtIGEgTGljZW7Dp2EgQ3JlYXRpdmUgY29tbW9ucy4= |
dc.title.pt_BR.fl_str_mv |
Astrovírus infection in hospitalized children: molecular, clinical and epidemiological features |
title |
Astrovírus infection in hospitalized children: molecular, clinical and epidemiological features |
spellingShingle |
Astrovírus infection in hospitalized children: molecular, clinical and epidemiological features Siqueira, Jones Anderson Monteiro Astroviridae / patogenicidade Gastroenterite Mamastrovirus / patogenicidade Diarreia Região Amazônica (BR) Belém (PA) |
title_short |
Astrovírus infection in hospitalized children: molecular, clinical and epidemiological features |
title_full |
Astrovírus infection in hospitalized children: molecular, clinical and epidemiological features |
title_fullStr |
Astrovírus infection in hospitalized children: molecular, clinical and epidemiological features |
title_full_unstemmed |
Astrovírus infection in hospitalized children: molecular, clinical and epidemiological features |
title_sort |
Astrovírus infection in hospitalized children: molecular, clinical and epidemiological features |
author |
Siqueira, Jones Anderson Monteiro |
author_facet |
Siqueira, Jones Anderson Monteiro Oliveira, Darleise de Souza Carvalho, Thaís Cristina Nascimento de Portal, Thayara Morais Justino, Maria Cleonice Aguiar Silva, Luciana Damascena da Resque, Hugo Reis Gabbay, Yvone Benchimol |
author_role |
author |
author2 |
Oliveira, Darleise de Souza Carvalho, Thaís Cristina Nascimento de Portal, Thayara Morais Justino, Maria Cleonice Aguiar Silva, Luciana Damascena da Resque, Hugo Reis Gabbay, Yvone Benchimol |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Siqueira, Jones Anderson Monteiro Oliveira, Darleise de Souza Carvalho, Thaís Cristina Nascimento de Portal, Thayara Morais Justino, Maria Cleonice Aguiar Silva, Luciana Damascena da Resque, Hugo Reis Gabbay, Yvone Benchimol |
dc.subject.decsPrimary.pt_BR.fl_str_mv |
Astroviridae / patogenicidade Gastroenterite Mamastrovirus / patogenicidade Diarreia Região Amazônica (BR) Belém (PA) |
topic |
Astroviridae / patogenicidade Gastroenterite Mamastrovirus / patogenicidade Diarreia Região Amazônica (BR) Belém (PA) |
description |
Background: Astrovirus (HAstV) is a common viral pathogen that causes gastroenteritis worldwide. It is classified into eight classical human types (HAstV-1/8) and seven other less prevalent types, described as HAstV VA1, VA2, VA3, VA4, MLB-1, MLB-2 and MLB-3. During outbreaks, the elderly and children are the most affected, and the spread of the virus is associated with person-to-person contact, food ingestion and contaminated water. Objectives: The aim of the present study was to investigate the prevalence of infection and genetic diversity of HAstV strains. Samples were collected from children with acute gastroenteritis admitted to a large pediatric hospital during a surveillance period of three years (2008–2011) in Belém city, Pará State, Amazon Region, Northern Brazil. Study design: Screening and genotyping tests were conducted using RT-PCR to detect the classical and non-classical HAstV types using specific primers. A semi-nested RT-PCR protocol was developed to improve viral detection in samples with a low viral load. Results: The overall positivity observed in this study was 3.9% (19/483). The age distribution showed a high prevalence of positive cases in children under one year old (5.3%). We found vomiting associated with 75% of the positive cases, fever with 82.3%, and dehydration with 76.9%. Most patients with positive cases demonstrated two to five days of diarrhea, two to three episodes of vomiting during hospitalization, and three bowel movements per day. Co-infection with HAstV and norovirus was observed in three cases (15.8%), and no pattern of seasonality or any relationship between the HAstV positivity rate and climate variables was observed. Eighteen positive samples (94.7%–18/19) were genotyped based on the ORF 2 region, and the greatest prevalence was of HAstV-1a (66.6%–12/18), followed by HAstV-2 (22.2%–4/18, comprising two type-2b and two type-2c genotypes), HAstV-3c (5.6%–1/18) and HAstV-4c (5.6%–1/18). No non-classical types were detected in the clinical samples analyzed. Conclusions: The present study showed that although HAstV infections occur at low frequency, they are involved in severe pediatric cases of acute gastroenteritis presenting with a high diversity of strains, including the lineages 3c and 4c, which were never before detected in Brazil. |
publishDate |
2017 |
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2017-11-24T16:28:06Z |
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SIQUEIRA, Jones Anderson Monteiro et al. Astrovírus infection in hospitalized children: molecular, clinical and epidemiological features. Journal of Clinical Virology, v. 94, p. 79-85, Sept. 2017. |
dc.identifier.uri.fl_str_mv |
https://patua.iec.gov.br/handle/iec/2880 |
dc.identifier.issn.-.fl_str_mv |
1386-6532 |
dc.identifier.doi.-.fl_str_mv |
10.1016/j.jcv.2017.07.014 |
identifier_str_mv |
SIQUEIRA, Jones Anderson Monteiro et al. Astrovírus infection in hospitalized children: molecular, clinical and epidemiological features. Journal of Clinical Virology, v. 94, p. 79-85, Sept. 2017. 1386-6532 10.1016/j.jcv.2017.07.014 |
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Elsevier |
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Elsevier |
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