Association of breast cancer with human papillomavirus (HPV) infection in Northeast Brazil: molecular evidence

• Autor(a) principal: Cavalcante, José Roosevelt
• Data de Publicação: 2018
• Outros Autores: Pinheiro, Luiz Gonzaga Porto, Almeida, Paulo Roberto Carvalho de, Ferreira, Márcia Valéria Pitombeira, Cruz, Gizele Almada, Campelo, Thales Alves, Silva, Caroliny Soares, Lima, Luana Nepomuceno Gondim Costa, Oliveira, Bruno Masato Kitagawa de, Lima, Lara Mulato, Feitosa, Laura Magda Costa, Pinheiro, Agostinho Câmara, Frota, Cristiane Cunha
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Digital do Instituto Evandro Chagas (Patuá)
• Texto Completo: https://patua.iec.gov.br/handle/iec/3525
• topic: Papillomaviridae / patogenicidade; Infecções por Papillomavirus / patogenicidade; Papillomavirus Humano 11 / isolamento & purificação; Papillomavirus Humano 6 / isolamento & purificação; Neoplasias da Mama; Carcinogênese / patologia; Coinfecção

OBJECTIVE: The aim of this study is to investigate the presence of human papillomavirus DNA and genotypes in breast cancer and normal breast tissue samples obtained from women from the northeast region of Brazil. METHOD: One hundred three breast cancer samples and 95 normal breast samples, as the non-malignant controls, were studied. DNA extraction was verified by human beta-globin gene amplification, and polymerase chain reaction was conducted based on HPV L1-specific consensus primers MY09/MY11 and GP5+/GP6+, followed by nested multiplex polymerase chain reaction with type-specific primers for the E6/E7 consensus region. RESULTS: Human papillomavirus DNA was detected in 51 (49.5%) breast carcinoma samples and 15 (15.8%) normal breast samples (p<0.0001). Human papillomavirus genotypes 6 and 11 were identified in 15.2% of all samples. CONCLUSIONS: The high frequency of human papillomavirus infection in breast cancer samples indicates a potential role of this virus in breast carcinogenesis in the studied participants.