Comparative cytogenetics between two important songbird, models: the Zebra Finch and the Canary
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Digital do Instituto Evandro Chagas (Patuá) |
Texto Completo: | https://patua.iec.gov.br/handle/iec/2534 |
Resumo: | Songbird species (order Passeriformes, suborder Oscines) are important models in various experimental fields spanning behavioural genomics to neurobiology. Although the genomes of some songbird species were sequenced recently, the chromosomal organization of these species is mostly unknown. Here we focused on the two most studied songbird species in neuroscience, the zebra finch (Taeniopygia guttata) and the canary (Serinus canaria). In order to clarify these issues and also to integrate chromosome data with their assembled genomes, we used classical and molecular cytogenetics in both zebra finch and canary to define their chromosomal homology, localization of heterochromatic blocks and distribution of rDNA clusters. We confirmed the same diploid number (2n = 80) in both species, as previously reported. FISH experiments confirmed the occurrence of multiple paracentric and pericentric inversions previously found in other species of Passeriformes, providing a cytogenetic signature for this order, and corroborating data from in silico analyses. Additionally, compared to other Passeriformes, we detected differences in the zebra finch karyotype concerning the morphology of some chromosomes, in the distribution of 5S rDNA clusters, and an inversion in chromosome 1. |
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Santos, Michelly da Silva dosKretschmer, RafaelFrankl-Vilches, CarolinaBakker, AntjeGahr, ManfredO' Brien, Patricia C. MFerguson-Smith, Malcolm AOliveira, Edivaldo Herculano Correa de2017-02-20T18:43:10Z2017-02-20T18:43:10Z2017SANTOS, Michelly da Silva dos et al. Comparative cytogenetics between two important songbird, models: the Zebra Finch and the Canary. PLoS One, v. 12, n. 1, 2017.1932-6203https://patua.iec.gov.br/handle/iec/253410.1371/journal.pone.0170997Songbird species (order Passeriformes, suborder Oscines) are important models in various experimental fields spanning behavioural genomics to neurobiology. Although the genomes of some songbird species were sequenced recently, the chromosomal organization of these species is mostly unknown. Here we focused on the two most studied songbird species in neuroscience, the zebra finch (Taeniopygia guttata) and the canary (Serinus canaria). In order to clarify these issues and also to integrate chromosome data with their assembled genomes, we used classical and molecular cytogenetics in both zebra finch and canary to define their chromosomal homology, localization of heterochromatic blocks and distribution of rDNA clusters. We confirmed the same diploid number (2n = 80) in both species, as previously reported. FISH experiments confirmed the occurrence of multiple paracentric and pericentric inversions previously found in other species of Passeriformes, providing a cytogenetic signature for this order, and corroborating data from in silico analyses. Additionally, compared to other Passeriformes, we detected differences in the zebra finch karyotype concerning the morphology of some chromosomes, in the distribution of 5S rDNA clusters, and an inversion in chromosome 1.Universidade Federal do Pará. Instituto de Ciências Biológicas. Programa de Pós-Graduação em Genética e Biologia Molecular. Belém, PA, Brazil.Universidade Federal do Rio Grande do Sul. Programa de Pós-Graduação em Genética e Biologia Molecular. Porto Alegre, RS, Brazil.Max Planck Institute for Ornithology. Department of Behavioral Neurobiology. Seewiesen, Germany.Max Planck Institute for Ornithology. Department of Behavioral Neurobiology. Seewiesen, Germany.Max Planck Institute for Ornithology. Department of Behavioral Neurobiology. Seewiesen, Germany.University of Cambridge. Department of Veterinary Medicine. Cambridge Resource Centre for Comparative Genomics. Cambridge, United Kingdom.University of Cambridge. Department of Veterinary Medicine. Cambridge Resource Centre for Comparative Genomics. Cambridge, United Kingdom.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Laboratório de Cultura de Tecidos e Citogenética. Ananindeua, PA, Brasil / Universidade Federal do Pará. Instituto de Ciências Exatas e Naturais. Faculdade de Ciências Naturais. Belém, PA, Brazil.engPublic Library of ScienceComparative cytogenetics between two important songbird, models: the Zebra Finch and the Canaryinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleAves Canoras / anatomia & histologiaPassarinho-zebraCanárioMapeamento CromossômicoPasseriformesCitogenéticainfo:eu-repo/semantics/openAccessreponame:Repositório Digital do Instituto Evandro Chagas (Patuá)instname:Instituto Evandro Chagas (IEC)instacron:IECTHUMBNAILComparative cytogenetics between two important songbird, models: the Zebra Finch and the Canary.pdf.jpgComparative cytogenetics between two important songbird, models: the Zebra Finch and the Canary.pdf.jpgGenerated Thumbnailimage/jpeg5816https://patua.iec.gov.br/bitstreams/005436c1-3b02-4fb9-8f30-10ef3bcded82/downloadc68f91589a3e6c67f8415211413a153eMD57ORIGINALComparative cytogenetics between two important songbird, models: the Zebra Finch and the Canary.pdfComparative cytogenetics between two important songbird, models: the Zebra Finch and the Canary.pdfapplication/pdf1685865https://patua.iec.gov.br/bitstreams/5e6f670c-c5f7-41bf-b225-84c60d9faab1/download5cf85f6aed8bddd4597d7e298493b61bMD51LICENSElicense.txtlicense.txttext/plain; charset=utf-871https://patua.iec.gov.br/bitstreams/7efc4f18-b126-4c1b-b93b-df56f707877c/download52f1732ea66fbd1123abe39f5373b797MD52TEXThoje soa7 meio ambientejournal.pone.0170997.pdf.txthoje soa7 meio ambientejournal.pone.0170997.pdf.txtExtracted texttext/plain38500https://patua.iec.gov.br/bitstreams/c6477225-8b22-489d-91db-958b5ba82007/downloadadf73af925725bcad7e0255308f94326MD53Comparative cytogenetics between two important songbird, models: the Zebra Finch and the Canary.pdf.txtComparative cytogenetics between two important songbird, models: the Zebra Finch and the Canary.pdf.txtExtracted texttext/plain42967https://patua.iec.gov.br/bitstreams/4a9ebdb2-3864-49a5-9dc4-af815c38061a/download1bff49900bcef7fc0ce7b57b1d4fbf0bMD56iec/25342022-10-20 21:10:29.345oai:patua.iec.gov.br:iec/2534https://patua.iec.gov.brRepositório InstitucionalPUBhttps://patua.iec.gov.br/oai/requestclariceneta@iec.gov.br || Biblioteca@iec.gov.bropendoar:2022-10-20T21:10:29Repositório Digital do Instituto Evandro Chagas (Patuá) - Instituto Evandro Chagas (IEC)falseVG9kb3Mgb3MgZG9jdW1lbnRvcyBkZXNzYSBjb2xlw6fDo28gc2VndWVtIGEgTGljZW7Dp2EgQ3JlYXRpdmUgY29tbW9ucy4= |
dc.title.pt_BR.fl_str_mv |
Comparative cytogenetics between two important songbird, models: the Zebra Finch and the Canary |
title |
Comparative cytogenetics between two important songbird, models: the Zebra Finch and the Canary |
spellingShingle |
Comparative cytogenetics between two important songbird, models: the Zebra Finch and the Canary Santos, Michelly da Silva dos Aves Canoras / anatomia & histologia Passarinho-zebra Canário Mapeamento Cromossômico Passeriformes Citogenética |
title_short |
Comparative cytogenetics between two important songbird, models: the Zebra Finch and the Canary |
title_full |
Comparative cytogenetics between two important songbird, models: the Zebra Finch and the Canary |
title_fullStr |
Comparative cytogenetics between two important songbird, models: the Zebra Finch and the Canary |
title_full_unstemmed |
Comparative cytogenetics between two important songbird, models: the Zebra Finch and the Canary |
title_sort |
Comparative cytogenetics between two important songbird, models: the Zebra Finch and the Canary |
author |
Santos, Michelly da Silva dos |
author_facet |
Santos, Michelly da Silva dos Kretschmer, Rafael Frankl-Vilches, Carolina Bakker, Antje Gahr, Manfred O' Brien, Patricia C. M Ferguson-Smith, Malcolm A Oliveira, Edivaldo Herculano Correa de |
author_role |
author |
author2 |
Kretschmer, Rafael Frankl-Vilches, Carolina Bakker, Antje Gahr, Manfred O' Brien, Patricia C. M Ferguson-Smith, Malcolm A Oliveira, Edivaldo Herculano Correa de |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Santos, Michelly da Silva dos Kretschmer, Rafael Frankl-Vilches, Carolina Bakker, Antje Gahr, Manfred O' Brien, Patricia C. M Ferguson-Smith, Malcolm A Oliveira, Edivaldo Herculano Correa de |
dc.subject.decsPrimary.pt_BR.fl_str_mv |
Aves Canoras / anatomia & histologia Passarinho-zebra Canário Mapeamento Cromossômico Passeriformes Citogenética |
topic |
Aves Canoras / anatomia & histologia Passarinho-zebra Canário Mapeamento Cromossômico Passeriformes Citogenética |
description |
Songbird species (order Passeriformes, suborder Oscines) are important models in various experimental fields spanning behavioural genomics to neurobiology. Although the genomes of some songbird species were sequenced recently, the chromosomal organization of these species is mostly unknown. Here we focused on the two most studied songbird species in neuroscience, the zebra finch (Taeniopygia guttata) and the canary (Serinus canaria). In order to clarify these issues and also to integrate chromosome data with their assembled genomes, we used classical and molecular cytogenetics in both zebra finch and canary to define their chromosomal homology, localization of heterochromatic blocks and distribution of rDNA clusters. We confirmed the same diploid number (2n = 80) in both species, as previously reported. FISH experiments confirmed the occurrence of multiple paracentric and pericentric inversions previously found in other species of Passeriformes, providing a cytogenetic signature for this order, and corroborating data from in silico analyses. Additionally, compared to other Passeriformes, we detected differences in the zebra finch karyotype concerning the morphology of some chromosomes, in the distribution of 5S rDNA clusters, and an inversion in chromosome 1. |
publishDate |
2017 |
dc.date.accessioned.fl_str_mv |
2017-02-20T18:43:10Z |
dc.date.available.fl_str_mv |
2017-02-20T18:43:10Z |
dc.date.issued.fl_str_mv |
2017 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
SANTOS, Michelly da Silva dos et al. Comparative cytogenetics between two important songbird, models: the Zebra Finch and the Canary. PLoS One, v. 12, n. 1, 2017. |
dc.identifier.uri.fl_str_mv |
https://patua.iec.gov.br/handle/iec/2534 |
dc.identifier.issn.-.fl_str_mv |
1932-6203 |
dc.identifier.doi.-.fl_str_mv |
10.1371/journal.pone.0170997 |
identifier_str_mv |
SANTOS, Michelly da Silva dos et al. Comparative cytogenetics between two important songbird, models: the Zebra Finch and the Canary. PLoS One, v. 12, n. 1, 2017. 1932-6203 10.1371/journal.pone.0170997 |
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https://patua.iec.gov.br/handle/iec/2534 |
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eng |
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eng |
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Public Library of Science |
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Public Library of Science |
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