Zika Virus epidemic in Brazil. II. Post-mortem analyses of neonates with microcephaly, stillbirths, and miscarriage

• Autor(a) principal: Azevedo, Raimunda do Socorro da Silva
• Data de Publicação: 2018
• Outros Autores: Araújo, Marialva Tereza Ferreira de, Oliveira, Consuelo Silva de, Martins Filho, Arnaldo Jorge, Nunes, Bruno Tardelli Diniz, Henriques, Daniele Freitas, Silva, Eliana Vieira Pinto da, Carvalho, Valéria Lima, Chiang, Jannifer Oliveira, Martins, Lívia Caricio, Vasconcelos, Barbara C. B, Sousa, Jorge R, Araújo, Fernanda Montenegro de Carvalho, Ribeiro, Erlane M, Castro, Andressa R. P, Queiroz, Maria Goretti Lins de, Verotti, Mariana P, Nunes, Márcio Roberto Teixeira, Cruz, Ana Cecília Ribeiro, Rodrigues, Sueli Guerreiro, Shi, Pei-Yong, Quaresma, Juarez Antônio Simões, Tesh, Robert B, Vasconcelos, Pedro Fernando da Costa
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Digital do Instituto Evandro Chagas (Patuá)
• Texto Completo: https://patua.iec.gov.br/handle/iec/3631
• topic: Vírus Zika; Zika virus / patogenicidade; Infecção pelo Zika virus / complicações; Infecção pelo Zika virus / mortalidade; Infecção pelo Zika virus / patologia; Microcefalia; Imuno-Histoquímica / métodos; Imunofluorescência / métodos; Reação em Cadeia da Polimerase Via Transcriptase Reversa / métodos; Morte Fetal; Recém-Nascido

The recent Zika virus(ZIKV) epidemic in Brazil was characterized by a range of different clinical presentations, particularly microcephaly, Guillain-Barré syndrome, and death. In this context, we determined the causal relationship between fatal microcephaly cases and ZIKV infection. Methods: Twelve fatal cases of neonates, whose mothers were infected with ZIKV during pregnancy, were examined; cases included nine neonatal deaths due to microcephaly, one miscarriage, and two stillbirths. Tissue samples were obtained from all cases at necropsy and were submitted for virological investigation (RT-qPCR and virus isolation) and/or histopathology (hematoxylin and eosin staining) and immunohistochemical assay for the detection of ZIKV antigens. Results: ZIKV antigens and/or ZIKV RNA were detected in tissue samples of all 12 cases examined. ZIKV was recovered in one case. Results of the virological and immunohistochemical analyses, as well as the anatomic abnormalities and histopathologic changes observed at necropsy on the 12 fatal cases, are presented. Conclusions: Data from these 12 cases provide strong evidence of the causal relationship between ZIKV and congenital disease in fetuses of women who were infected with the virus during pregnancy.