Granulocyte macrophage colony-stimulating factor alone reduces Toxoplasma gondii replication in microglial culture by superoxide and nitric oxide, without IFN-γ production: a preliminary report
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2018 |
| Outros Autores: | , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Digital do Instituto Evandro Chagas (Patuá) |
| Texto Completo: | https://patua.iec.gov.br/handle/iec/3193 |
Resumo: | In vitro studies have demonstrated that GM-CSF in combination with other stimulatory factors induces a microbicidal response that control T. gondii infection. We assessed whether GM-CSF alone can control T. gondii replication in murine microglial cultures. Microglia were collected and cultured with or without GM-CSF and the half of each group was infected with T. gondii. We determined the T. gondii infectivity, cytokines levels, NO and superoxide detection. GM-CSF alone primes microglia, which after infection induces the production of TNF-α and IL-6, leading to NO and superoxide production, without any stimulus from IL-12p70 and IFN-γ. |
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Pimenta, Tamirys SimãoChaves, Natalie FerreiraRodrigues, Ana Paula DrummondDiniz, Cristovam Wanderley PicançoDaMatta, Renato AugustoDiniz Junior, José Antônio Picanço2018-07-09T13:53:12Z2018-07-09T13:53:12Z2018PIMENTA, Tamirys Simão et al. Granulocyte macrophage colony-stimulating factor alone reduces Toxoplasma gondii replication in microglial culture by superoxide and nitric oxide, without IFN-γ production: a preliminary report. Microbes and Infection, v. 20, n. 6, p. 385-390, June - July 2018. DOI: https://doi.org/10.1016/j.micinf.2018.05.0061286-4579https://patua.iec.gov.br/handle/iec/319310.1016/j.micinf.2018.05.006In vitro studies have demonstrated that GM-CSF in combination with other stimulatory factors induces a microbicidal response that control T. gondii infection. We assessed whether GM-CSF alone can control T. gondii replication in murine microglial cultures. Microglia were collected and cultured with or without GM-CSF and the half of each group was infected with T. gondii. We determined the T. gondii infectivity, cytokines levels, NO and superoxide detection. GM-CSF alone primes microglia, which after infection induces the production of TNF-α and IL-6, leading to NO and superoxide production, without any stimulus from IL-12p70 and IFN-γ.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil / Federal University of Pará. Postgraduate Program in Neuroscience and Cell Biology. Belém, PA, Brazil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Laboratório de Microscopia eletrônica. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Laboratório de Microscopia eletrônica. Ananindeua, PA, Brasil.Federal University of Pará. Institute of Biological Sciences. University Hospital João de Barros Barreto. Laboratory of Investigations in Neurodegeneration and Infection. Belém, PA, Brazil.State University of North Fluminense. Center of Biosciences and Biotechnology. Laboratory of Cell and Tissue Biology. Goytacazes, RJ, Brazil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Laboratório de Microscopia eletrônica. Ananindeua, PA, Brasil.engElsevierGranulocyte macrophage colony-stimulating factor alone reduces Toxoplasma gondii replication in microglial culture by superoxide and nitric oxide, without IFN-γ production: a preliminary reportinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleToxoplasma / patogenicidadeFator Estimulador de Colônias de Granulócitos e MacrófagosMicroglia / citologiaÓxido Nítrico / síntese químicaSuperóxidos / síntese químicainfo:eu-repo/semantics/embargoedAccessreponame:Repositório Digital do Instituto Evandro Chagas (Patuá)instname:Instituto Evandro Chagas (IEC)instacron:IECORIGINALAcesso Embargado.pdfAcesso Embargado.pdfapplication/pdf551083https://patua.iec.gov.br/bitstreams/36d90817-b9b2-4a5f-a99c-34b028600eb8/downloadc9a9c128e29cac82a5d7fdf3f4e6da73MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-871https://patua.iec.gov.br/bitstreams/e930f3dd-6656-4e0d-94aa-8349ed73d0cb/download52f1732ea66fbd1123abe39f5373b797MD52TEXTGranulocyte macrophage colony-stimulating factor alone reduces Toxoplasma gondii replication in microglial culture by superoxide and nitric oxide, without IFN-γ production: a preliminary report.pdf.txtGranulocyte macrophage colony-stimulating factor alone reduces Toxoplasma gondii replication in microglial culture by superoxide and nitric oxide, without IFN-γ production: a preliminary report.pdf.txtExtracted texttext/plain2https://patua.iec.gov.br/bitstreams/8ea7df28-06d5-4214-b019-a3d4c032b90a/downloade1c06d85ae7b8b032bef47e42e4c08f9MD55Acesso Embargado.pdf.txtAcesso Embargado.pdf.txtExtracted texttext/plain2https://patua.iec.gov.br/bitstreams/8911d577-f3f1-4d13-b04c-7d0af47ca011/downloade1c06d85ae7b8b032bef47e42e4c08f9MD57THUMBNAILGranulocyte macrophage colony-stimulating factor alone reduces Toxoplasma gondii replication in microglial culture by superoxide and nitric oxide, without IFN-γ production: a preliminary report.pdf.jpgGranulocyte macrophage colony-stimulating factor alone reduces Toxoplasma gondii replication in microglial culture by superoxide and nitric oxide, without IFN-γ production: a preliminary report.pdf.jpgGenerated Thumbnailimage/jpeg3095https://patua.iec.gov.br/bitstreams/40e57adb-971f-4be0-b291-34fd6760daea/download71859d578212107f7f8c49a4ce09d9eeMD56Acesso Embargado.pdf.jpgAcesso Embargado.pdf.jpgGenerated Thumbnailimage/jpeg3095https://patua.iec.gov.br/bitstreams/d366beaa-8717-4448-9bdd-fc9202c1c674/download71859d578212107f7f8c49a4ce09d9eeMD58iec/31932023-06-19 13:21:41.309oai:patua.iec.gov.br:iec/3193https://patua.iec.gov.brRepositório InstitucionalPUBhttps://patua.iec.gov.br/oai/requestclariceneta@iec.gov.br || Biblioteca@iec.gov.bropendoar:2023-06-19T13:21:41Repositório Digital do Instituto Evandro Chagas (Patuá) - Instituto Evandro Chagas (IEC)falseVG9kb3Mgb3MgZG9jdW1lbnRvcyBkZXNzYSBjb2xlw6fDo28gc2VndWVtIGEgTGljZW7Dp2EgQ3JlYXRpdmUgY29tbW9ucy4= |
| dc.title.pt_BR.fl_str_mv |
Granulocyte macrophage colony-stimulating factor alone reduces Toxoplasma gondii replication in microglial culture by superoxide and nitric oxide, without IFN-γ production: a preliminary report |
| title |
Granulocyte macrophage colony-stimulating factor alone reduces Toxoplasma gondii replication in microglial culture by superoxide and nitric oxide, without IFN-γ production: a preliminary report |
| spellingShingle |
Granulocyte macrophage colony-stimulating factor alone reduces Toxoplasma gondii replication in microglial culture by superoxide and nitric oxide, without IFN-γ production: a preliminary report Pimenta, Tamirys Simão Toxoplasma / patogenicidade Fator Estimulador de Colônias de Granulócitos e Macrófagos Microglia / citologia Óxido Nítrico / síntese química Superóxidos / síntese química |
| title_short |
Granulocyte macrophage colony-stimulating factor alone reduces Toxoplasma gondii replication in microglial culture by superoxide and nitric oxide, without IFN-γ production: a preliminary report |
| title_full |
Granulocyte macrophage colony-stimulating factor alone reduces Toxoplasma gondii replication in microglial culture by superoxide and nitric oxide, without IFN-γ production: a preliminary report |
| title_fullStr |
Granulocyte macrophage colony-stimulating factor alone reduces Toxoplasma gondii replication in microglial culture by superoxide and nitric oxide, without IFN-γ production: a preliminary report |
| title_full_unstemmed |
Granulocyte macrophage colony-stimulating factor alone reduces Toxoplasma gondii replication in microglial culture by superoxide and nitric oxide, without IFN-γ production: a preliminary report |
| title_sort |
Granulocyte macrophage colony-stimulating factor alone reduces Toxoplasma gondii replication in microglial culture by superoxide and nitric oxide, without IFN-γ production: a preliminary report |
| author |
Pimenta, Tamirys Simão |
| author_facet |
Pimenta, Tamirys Simão Chaves, Natalie Ferreira Rodrigues, Ana Paula Drummond Diniz, Cristovam Wanderley Picanço DaMatta, Renato Augusto Diniz Junior, José Antônio Picanço |
| author_role |
author |
| author2 |
Chaves, Natalie Ferreira Rodrigues, Ana Paula Drummond Diniz, Cristovam Wanderley Picanço DaMatta, Renato Augusto Diniz Junior, José Antônio Picanço |
| author2_role |
author author author author author |
| dc.contributor.author.fl_str_mv |
Pimenta, Tamirys Simão Chaves, Natalie Ferreira Rodrigues, Ana Paula Drummond Diniz, Cristovam Wanderley Picanço DaMatta, Renato Augusto Diniz Junior, José Antônio Picanço |
| dc.subject.decsPrimary.pt_BR.fl_str_mv |
Toxoplasma / patogenicidade Fator Estimulador de Colônias de Granulócitos e Macrófagos Microglia / citologia Óxido Nítrico / síntese química Superóxidos / síntese química |
| topic |
Toxoplasma / patogenicidade Fator Estimulador de Colônias de Granulócitos e Macrófagos Microglia / citologia Óxido Nítrico / síntese química Superóxidos / síntese química |
| description |
In vitro studies have demonstrated that GM-CSF in combination with other stimulatory factors induces a microbicidal response that control T. gondii infection. We assessed whether GM-CSF alone can control T. gondii replication in murine microglial cultures. Microglia were collected and cultured with or without GM-CSF and the half of each group was infected with T. gondii. We determined the T. gondii infectivity, cytokines levels, NO and superoxide detection. GM-CSF alone primes microglia, which after infection induces the production of TNF-α and IL-6, leading to NO and superoxide production, without any stimulus from IL-12p70 and IFN-γ. |
| publishDate |
2018 |
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2018-07-09T13:53:12Z |
| dc.date.available.fl_str_mv |
2018-07-09T13:53:12Z |
| dc.date.issued.fl_str_mv |
2018 |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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publishedVersion |
| dc.identifier.citation.fl_str_mv |
PIMENTA, Tamirys Simão et al. Granulocyte macrophage colony-stimulating factor alone reduces Toxoplasma gondii replication in microglial culture by superoxide and nitric oxide, without IFN-γ production: a preliminary report. Microbes and Infection, v. 20, n. 6, p. 385-390, June - July 2018. DOI: https://doi.org/10.1016/j.micinf.2018.05.006 |
| dc.identifier.uri.fl_str_mv |
https://patua.iec.gov.br/handle/iec/3193 |
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1286-4579 |
| dc.identifier.doi.-.fl_str_mv |
10.1016/j.micinf.2018.05.006 |
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PIMENTA, Tamirys Simão et al. Granulocyte macrophage colony-stimulating factor alone reduces Toxoplasma gondii replication in microglial culture by superoxide and nitric oxide, without IFN-γ production: a preliminary report. Microbes and Infection, v. 20, n. 6, p. 385-390, June - July 2018. DOI: https://doi.org/10.1016/j.micinf.2018.05.006 1286-4579 10.1016/j.micinf.2018.05.006 |
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https://patua.iec.gov.br/handle/iec/3193 |
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eng |
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Elsevier |
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Elsevier |
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