Serum cytokine responses over the entire clinical-immunological spectrum of human Leishmania (L.) infantum chagasi infection

• Autor(a) principal: Ramos, Patrícia Karla Santos
• Data de Publicação: 2017
• Outros Autores: Carvalho, Karina Inácio, Rosa, Daniela Santoro, Rodrigues, Ana Paula, Lima, Luciana Vieira do Rêgo, Campos, Marliane Batista, Gomes, Claudia Maria de Castro, Laurenti, Márcia Dalastra, Corbett, Carlos Eduardo Pereira, Silveira, Fernando Tobias
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Digital do Instituto Evandro Chagas (Patuá)
• Texto Completo: https://patua.iec.gov.br/handle/iec/2441
• topic: Citocinas / análise; Interleucina-6 / imunologia; Interleucina-10 / imunologia; Leishmania infantum / imunologia; Leishmaniose Visceral; Estudos Transversais / métodos; Ecossistema Amazônico; Brasil (BR)

The clinical-immunological spectrum of human Leishmania (L.) infantum chagasi infection in Amazonian Brazil was recently reviewed based on clinical, DTH, and IFAT (IgG) evaluations that identified five profiles: three asymptomatic (asymptomatic infection, AI; subclinical resistant infection, SRI; and indeterminate initial infection, III) and two symptomatic (symptomatic infection, SI; American visceral leishmaniasis, AVL; and subclinical oligosymptomatic infection, SOI). TNF-α, IL-4, IL-6, and IL-10 serum cytokines were analyzed using multiplexed Cytometric Bead Array in 161 samples from endemic areas in the Brazilian Amazon: SI [AVL] (21 cases), III (49), SRI (19), SOI (12), AI (36), and a control group [CG] (24). The highest IL-6 serum levels were observed in the SI profile (AVL); higher IL-10 serum levels were observed in SI than in SOI or CG and in AI and III than in SOI; higher TNF-α serum levels were seen in SI than in CG. Positive correlations were found between IL-6 and IL-10 serum levels in the SI and III profiles and between IL-6 and TNF-α and between IL-4 and TNF-α in the III profile. These results provide strong evidence for associating IL-6 and IL-10 with the immunopathogenesis of AVL and help clarify the role of these cytokines in the infection spectrum.