Comparative analysis of carbohydrate residues in the midgut of phlebotomines (Diptera: Psychodidae) from colony and field populations from Amazon, Brazil
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Digital do Instituto Evandro Chagas (Patuá) |
Texto Completo: | https://patua.iec.gov.br/handle/iec/2478 |
Resumo: | Leishmaniasis are worldwide diseases that occur in 98 countries including Brazil, transmitted by the bite of female phlebotomines during blood feeding. In Brazil it is known that some species of sand flies as Lutzomyia longipalpis sensun latum (vector of Leishmania infantum chagasi), Lutzomyia flaviscutellata (vector of Leishmania (Leishmania) amazonensis) and Lutzomyia antunesi [suspected vector of Leishmania (Viannia) lindenbergi] are incriminated of transmitting the parasite Leishmania for the vertebrate host. The phlebotomine-parasite is mediated by the attachment of the promastigote lipophosphoglycan (LPG) to the midgut epithelium. However, another mechanism that is LPG-independent and mediated by Nacetyl-galactosamine (GalNAc) seems to occur in some species of phlebotomines that are classified as permissive. The aim of this study was to characterize the carbohydrate residues that, probably, play a role in parasite attachment to the midgut of phlebotomine from colony and field populations from the Brazilian Amazonian region. We observed the presence of GalNAc, mannose, galactose and GlcNAc in all phlebotomine species. A binding assay between L. (L.) amazonensis and L. i.chagasi to the midguts of different species of phlebotomines was performed. The attachment of both Leishmania and vector species. |
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Oliveira, Davi Marcos Souza deSilva, Bruno José Martins daSena, Chubert Bernardo Castro deLima, José Aprígio NunesSantos, Thiago Vasconcelos dosSilveira, Fernando TobiasSilva, Edilene Oliveira2017-02-06T12:11:16Z2017-02-06T12:11:16Z2016OLIVEIRA, Davi Marcos Souza de et al. Comparative analysis of carbohydrate residues in the midgut of phlebotomines (Diptera: Psychodidae) from colony and field populations from Amazon, Brazil. Experimental Parasitology, v. 168, p. 31-38, Sept. 2016. DOI: https://doi.org/10.1016/j.exppara.2016.06.0021090-2449https://patua.iec.gov.br/handle/iec/247810.1016/j.exppara.2016.06.002Leishmaniasis are worldwide diseases that occur in 98 countries including Brazil, transmitted by the bite of female phlebotomines during blood feeding. In Brazil it is known that some species of sand flies as Lutzomyia longipalpis sensun latum (vector of Leishmania infantum chagasi), Lutzomyia flaviscutellata (vector of Leishmania (Leishmania) amazonensis) and Lutzomyia antunesi [suspected vector of Leishmania (Viannia) lindenbergi] are incriminated of transmitting the parasite Leishmania for the vertebrate host. The phlebotomine-parasite is mediated by the attachment of the promastigote lipophosphoglycan (LPG) to the midgut epithelium. However, another mechanism that is LPG-independent and mediated by Nacetyl-galactosamine (GalNAc) seems to occur in some species of phlebotomines that are classified as permissive. The aim of this study was to characterize the carbohydrate residues that, probably, play a role in parasite attachment to the midgut of phlebotomine from colony and field populations from the Brazilian Amazonian region. We observed the presence of GalNAc, mannose, galactose and GlcNAc in all phlebotomine species. A binding assay between L. (L.) amazonensis and L. i.chagasi to the midguts of different species of phlebotomines was performed. The attachment of both Leishmania and vector species.Federal University of Para. Institute of Biological Sciences. Laboratory of Parasitology. Belém, PA, Brazil / Federal University of Para. Institute of Biological Sciences. Laboratory of Structural Biology, Belém, PA, Brazil / National Institute of Science and Technology for Structural Biology and Bioimaging, Rio de Janeiro, RJ, Brazil.Federal University of Para. Institute of Biological Sciences. Laboratory of Parasitology. Belém, PA, Brazil / Federal University of Para. Institute of Biological Sciences. Laboratory of Structural Biology, Belém, PA, Brazil / National Institute of Science and Technology for Structural Biology and Bioimaging, Rio de Janeiro, RJ, Brazil.Laboratory of Structural Biology, Belém, PA, Brazil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Laboratório de Leishmaniasis. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Laboratório de Leishmaniasis. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Laboratório de Leishmaniasis. Ananindeua, PA, Brasil / Federal University of Para. Tropical Medicine Nucleus. Belém, PA, Brazil.Federal University of Para. Institute of Biological Sciences. Laboratory of Parasitology. Belém, PA, Brazil / Federal University of Para. Institute of Biological Sciences. Laboratory of Structural Biology, Belém, PA, Brazil / National Institute of Science and Technology for Structural Biology and Bioimaging, Rio de Janeiro, RJ, Brazil.application/pdfengElsevierComparative analysis of carbohydrate residues in the midgut of phlebotomines (Diptera: Psychodidae) from colony and field populations from Amazon, BrazilAnálise comparativa de resíduos de carboidratos no intestino médio de flebotomíneos (Diptera: Psychodidae) de populações de colônias e campos da Amazôniainfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article20132015Leishmania / fisiologiaPsychodidae / fisiologiaCarboidratos / análiseGlicoconjugados / metabolismoLectinasIntestino DelgadoInterações Hospedeiro-ParasitaEcossistema AmazônicoBrasil (BR)info:eu-repo/semantics/embargoedAccessreponame:Repositório Digital do Instituto Evandro Chagas (Patuá)instname:Instituto Evandro Chagas (IEC)instacron:IECORIGINALAcesso Embargado.pdfAcesso Embargado.pdfapplication/pdf551083https://patua.iec.gov.br/bitstreams/d0104140-0d40-464b-a590-40894c3f3f31/downloadc9a9c128e29cac82a5d7fdf3f4e6da73MD54TEXTComparative analysis of carbohydrate residues in the midgut of phlebotomines (Diptera: Psychodidae) from colony and field populations from Amazon, Brazil.pdf.txtComparative analysis of carbohydrate residues in the midgut of phlebotomines (Diptera: Psychodidae) from colony and field populations from Amazon, Brazil.pdf.txtExtracted texttext/plain2https://patua.iec.gov.br/bitstreams/404886d7-ba42-430b-90a6-0d4d03d6ba17/downloade1c06d85ae7b8b032bef47e42e4c08f9MD58Acesso Embargado.pdf.txtAcesso Embargado.pdf.txtExtracted texttext/plain2https://patua.iec.gov.br/bitstreams/b908ba93-0e89-41e4-9f4a-ad6459e2e038/downloade1c06d85ae7b8b032bef47e42e4c08f9MD510THUMBNAILComparative analysis of carbohydrate residues in the midgut of phlebotomines (Diptera: Psychodidae) from colony and field populations from Amazon, Brazil.pdf.jpgComparative analysis of carbohydrate residues in the midgut of phlebotomines (Diptera: Psychodidae) from colony and field populations from Amazon, Brazil.pdf.jpgGenerated Thumbnailimage/jpeg3095https://patua.iec.gov.br/bitstreams/8dd14086-e5d3-4b48-b68d-33608cab7c8d/download71859d578212107f7f8c49a4ce09d9eeMD59Acesso Embargado.pdf.jpgAcesso Embargado.pdf.jpgGenerated Thumbnailimage/jpeg3095https://patua.iec.gov.br/bitstreams/d211e722-9d81-4d54-a6c8-9befdbd0b7db/download71859d578212107f7f8c49a4ce09d9eeMD511LICENSElicense.txtlicense.txttext/plain; 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dc.title.pt_BR.fl_str_mv |
Comparative analysis of carbohydrate residues in the midgut of phlebotomines (Diptera: Psychodidae) from colony and field populations from Amazon, Brazil |
dc.title.alternative.pt_BR.fl_str_mv |
Análise comparativa de resíduos de carboidratos no intestino médio de flebotomíneos (Diptera: Psychodidae) de populações de colônias e campos da Amazônia |
title |
Comparative analysis of carbohydrate residues in the midgut of phlebotomines (Diptera: Psychodidae) from colony and field populations from Amazon, Brazil |
spellingShingle |
Comparative analysis of carbohydrate residues in the midgut of phlebotomines (Diptera: Psychodidae) from colony and field populations from Amazon, Brazil Oliveira, Davi Marcos Souza de Leishmania / fisiologia Psychodidae / fisiologia Carboidratos / análise Glicoconjugados / metabolismo Lectinas Intestino Delgado Interações Hospedeiro-Parasita Ecossistema Amazônico Brasil (BR) |
title_short |
Comparative analysis of carbohydrate residues in the midgut of phlebotomines (Diptera: Psychodidae) from colony and field populations from Amazon, Brazil |
title_full |
Comparative analysis of carbohydrate residues in the midgut of phlebotomines (Diptera: Psychodidae) from colony and field populations from Amazon, Brazil |
title_fullStr |
Comparative analysis of carbohydrate residues in the midgut of phlebotomines (Diptera: Psychodidae) from colony and field populations from Amazon, Brazil |
title_full_unstemmed |
Comparative analysis of carbohydrate residues in the midgut of phlebotomines (Diptera: Psychodidae) from colony and field populations from Amazon, Brazil |
title_sort |
Comparative analysis of carbohydrate residues in the midgut of phlebotomines (Diptera: Psychodidae) from colony and field populations from Amazon, Brazil |
author |
Oliveira, Davi Marcos Souza de |
author_facet |
Oliveira, Davi Marcos Souza de Silva, Bruno José Martins da Sena, Chubert Bernardo Castro de Lima, José Aprígio Nunes Santos, Thiago Vasconcelos dos Silveira, Fernando Tobias Silva, Edilene Oliveira |
author_role |
author |
author2 |
Silva, Bruno José Martins da Sena, Chubert Bernardo Castro de Lima, José Aprígio Nunes Santos, Thiago Vasconcelos dos Silveira, Fernando Tobias Silva, Edilene Oliveira |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Oliveira, Davi Marcos Souza de Silva, Bruno José Martins da Sena, Chubert Bernardo Castro de Lima, José Aprígio Nunes Santos, Thiago Vasconcelos dos Silveira, Fernando Tobias Silva, Edilene Oliveira |
dc.subject.decsPrimary.pt_BR.fl_str_mv |
Leishmania / fisiologia Psychodidae / fisiologia Carboidratos / análise Glicoconjugados / metabolismo Lectinas Intestino Delgado Interações Hospedeiro-Parasita Ecossistema Amazônico Brasil (BR) |
topic |
Leishmania / fisiologia Psychodidae / fisiologia Carboidratos / análise Glicoconjugados / metabolismo Lectinas Intestino Delgado Interações Hospedeiro-Parasita Ecossistema Amazônico Brasil (BR) |
description |
Leishmaniasis are worldwide diseases that occur in 98 countries including Brazil, transmitted by the bite of female phlebotomines during blood feeding. In Brazil it is known that some species of sand flies as Lutzomyia longipalpis sensun latum (vector of Leishmania infantum chagasi), Lutzomyia flaviscutellata (vector of Leishmania (Leishmania) amazonensis) and Lutzomyia antunesi [suspected vector of Leishmania (Viannia) lindenbergi] are incriminated of transmitting the parasite Leishmania for the vertebrate host. The phlebotomine-parasite is mediated by the attachment of the promastigote lipophosphoglycan (LPG) to the midgut epithelium. However, another mechanism that is LPG-independent and mediated by Nacetyl-galactosamine (GalNAc) seems to occur in some species of phlebotomines that are classified as permissive. The aim of this study was to characterize the carbohydrate residues that, probably, play a role in parasite attachment to the midgut of phlebotomine from colony and field populations from the Brazilian Amazonian region. We observed the presence of GalNAc, mannose, galactose and GlcNAc in all phlebotomine species. A binding assay between L. (L.) amazonensis and L. i.chagasi to the midguts of different species of phlebotomines was performed. The attachment of both Leishmania and vector species. |
publishDate |
2016 |
dc.date.issued.fl_str_mv |
2016 |
dc.date.accessioned.fl_str_mv |
2017-02-06T12:11:16Z |
dc.date.available.fl_str_mv |
2017-02-06T12:11:16Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
OLIVEIRA, Davi Marcos Souza de et al. Comparative analysis of carbohydrate residues in the midgut of phlebotomines (Diptera: Psychodidae) from colony and field populations from Amazon, Brazil. Experimental Parasitology, v. 168, p. 31-38, Sept. 2016. DOI: https://doi.org/10.1016/j.exppara.2016.06.002 |
dc.identifier.uri.fl_str_mv |
https://patua.iec.gov.br/handle/iec/2478 |
dc.identifier.issn.-.fl_str_mv |
1090-2449 |
dc.identifier.doi.-.fl_str_mv |
10.1016/j.exppara.2016.06.002 |
identifier_str_mv |
OLIVEIRA, Davi Marcos Souza de et al. Comparative analysis of carbohydrate residues in the midgut of phlebotomines (Diptera: Psychodidae) from colony and field populations from Amazon, Brazil. Experimental Parasitology, v. 168, p. 31-38, Sept. 2016. DOI: https://doi.org/10.1016/j.exppara.2016.06.002 1090-2449 10.1016/j.exppara.2016.06.002 |
url |
https://patua.iec.gov.br/handle/iec/2478 |
dc.language.iso.fl_str_mv |
eng |
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eng |
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dc.coverage.temporalrageupto.-.fl_str_mv |
2015 |
dc.publisher.none.fl_str_mv |
Elsevier |
publisher.none.fl_str_mv |
Elsevier |
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