Extreme geographical fixation of variation in the Plasmodium falciparum gamete surface protein gene Pfs48/45 compared with microsatellite loci
Autor(a) principal: | |
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Data de Publicação: | 2001 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Digital do Instituto Evandro Chagas (Patuá) |
Texto Completo: | https://patua.iec.gov.br/handle/iec/4362 |
Resumo: | Comparing patterns of genetic variation at multiple loci in the genome of a species can potentially identify loci which are under selection. The large number of polymorphic microsatellites in the malaria parasite Plasmodium falciparum are available markers to screen for selectively important loci. The Pfs48/45 gene on Chromosome 13 encodes an antigenic protein located on the surface of parasite gametes, which is a candidate for a transmission blocking vaccine. Here, genotypic data from 255 P. falciparum isolates are presented, which show that alleles and haplotypes of five single nucleotide polymorphisms (SNPs) in the Pfs48/45 gene are exceptionally skewed in frequency among different P. falciparum populations, compared with alleles at 11 microsatellite loci sampled widely from the parasite genome. Fixation indices measuring inter-population variance in allele frequencies (FST) were in the order of four to seven times higher for Pfs48/45 than for the microsatellites, whether considered (i) among populations within Africa, or (ii) among different continents. Differing mutational processes at microsatellite and SNP loci could generally affect the population structure at these different types of loci, to an unknown extent which deserves further investigation. The highly contrasting population structure may also suggest divergent selection on the amino acid sequence of Pfs48/45 in different populations, which plausibly indicates a role for the protein in determining gamete recognition and compatibility. © 2001 Elsevier Science B.V. |
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Conway, David JMachado, Ricardo Luiz DantasSingh, BalbirDessert, PatriciaMikes, Zsuzsanna SPóvoa, Marinete MarinsOduola, Ayoade M. JRoper, Cally2021-07-19T22:28:43Z2021-07-19T22:28:43Z2001CONWAY, David J. et al. Extreme geographical fixation of variation in the Plasmodium falciparum gamete surface protein gene Pfs48/45 compared with microsatellite loci. Molecular and Biochemical Parasitology, v. 115, n. 2, p. 145-156, July 2001.0166-6851https://patua.iec.gov.br/handle/iec/436210.1016/s0166-6851(01)00278-x.Comparing patterns of genetic variation at multiple loci in the genome of a species can potentially identify loci which are under selection. The large number of polymorphic microsatellites in the malaria parasite Plasmodium falciparum are available markers to screen for selectively important loci. The Pfs48/45 gene on Chromosome 13 encodes an antigenic protein located on the surface of parasite gametes, which is a candidate for a transmission blocking vaccine. Here, genotypic data from 255 P. falciparum isolates are presented, which show that alleles and haplotypes of five single nucleotide polymorphisms (SNPs) in the Pfs48/45 gene are exceptionally skewed in frequency among different P. falciparum populations, compared with alleles at 11 microsatellite loci sampled widely from the parasite genome. Fixation indices measuring inter-population variance in allele frequencies (FST) were in the order of four to seven times higher for Pfs48/45 than for the microsatellites, whether considered (i) among populations within Africa, or (ii) among different continents. Differing mutational processes at microsatellite and SNP loci could generally affect the population structure at these different types of loci, to an unknown extent which deserves further investigation. The highly contrasting population structure may also suggest divergent selection on the amino acid sequence of Pfs48/45 in different populations, which plausibly indicates a role for the protein in determining gamete recognition and compatibility. © 2001 Elsevier Science B.V.Wellcome Trust (Grant Ref. 055487/Z/98/Z) and the Medical Research Council (Grant Ref. G9803180).London School of Hygiene and Tropical Medicine. Department of Infectious and Tropical Diseases. Keppel Street, London, UK.Ministério da Saúde. Fundação Nacional de Saúde. Instituto Evandro Chagas. Belém, PA, Brasil.Universiti Malaysia Sarawakc. Faculty of Medicine and Health Sciences. Kota Samarahan, Sarawak, Malaysia.London School of Hygiene and Tropical Medicine. Department of Infectious and Tropical Diseases. Keppel Street, London, UK.London School of Hygiene and Tropical Medicine. Department of Infectious and Tropical Diseases. Keppel Street, London, UK.Ministério da Saúde. Fundação Nacional de Saúde. Instituto Evandro Chagas. Belém, PA, Brasil.University of Ibadan. College of Medicine. Postgraduate Institute of Medical Research and Training. Ibadan, Nigeria.London School of Hygiene and Tropical Medicine. Department of Infectious and Tropical Diseases. Keppel Street, London, UK.engElsevierExtreme geographical fixation of variation in the Plasmodium falciparum gamete surface protein gene Pfs48/45 compared with microsatellite lociinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleMaláriaPlasmodium falciparum / parasitologiaGenoma / genéticaMarcadores GenéticosRepetições de Microssatélitesinfo:eu-repo/semantics/openAccessreponame:Repositório Digital do Instituto Evandro Chagas (Patuá)instname:Instituto Evandro Chagas (IEC)instacron:IECLICENSElicense.txtlicense.txttext/plain; charset=utf-82182https://patua.iec.gov.br/bitstreams/9008bad6-85ec-4870-816a-f2895dc10c5e/download11832eea31b16df8613079d742d61793MD52TEXTExtreme geographical fixation of variation in the Plasmodium falciparum gamete surface protein gene Pfs48/45 compared with microsatellite loci.pdf.txtExtreme geographical fixation of variation in the Plasmodium falciparum gamete surface protein gene Pfs48/45 compared with microsatellite loci.pdf.txtExtracted texttext/plain41828https://patua.iec.gov.br/bitstreams/b77c832f-386c-4ff6-9d8d-005e6b56fe6c/download0ab4caa11292748e7def187c067ac113MD55THUMBNAILExtreme geographical fixation of variation in the Plasmodium falciparum gamete surface protein gene Pfs48/45 compared with microsatellite loci.pdf.jpgExtreme geographical fixation of variation in the Plasmodium falciparum gamete surface protein gene Pfs48/45 compared with microsatellite loci.pdf.jpgGenerated Thumbnailimage/jpeg5720https://patua.iec.gov.br/bitstreams/72e09c4c-cefd-47cc-b4ad-6d3b4a6b4961/downloadb025b726d5e9c297ce106a16441eb456MD56ORIGINALExtreme geographical fixation of variation in the Plasmodium falciparum gamete surface protein gene Pfs48/45 compared with microsatellite loci.pdfExtreme geographical fixation of variation in the Plasmodium falciparum gamete surface protein gene Pfs48/45 compared with microsatellite loci.pdfapplication/pdf200932https://patua.iec.gov.br/bitstreams/d55276d3-27e0-4040-a925-900910fa509a/downloadd7c23b758dd037ada883eaabdb571560MD51iec/43622022-10-20 23:21:50.6oai:patua.iec.gov.br:iec/4362https://patua.iec.gov.brRepositório InstitucionalPUBhttps://patua.iec.gov.br/oai/requestclariceneta@iec.gov.br || Biblioteca@iec.gov.bropendoar:2022-10-20T23:21:50Repositório Digital do Instituto Evandro Chagas (Patuá) - 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dc.title.pt_BR.fl_str_mv |
Extreme geographical fixation of variation in the Plasmodium falciparum gamete surface protein gene Pfs48/45 compared with microsatellite loci |
title |
Extreme geographical fixation of variation in the Plasmodium falciparum gamete surface protein gene Pfs48/45 compared with microsatellite loci |
spellingShingle |
Extreme geographical fixation of variation in the Plasmodium falciparum gamete surface protein gene Pfs48/45 compared with microsatellite loci Conway, David J Malária Plasmodium falciparum / parasitologia Genoma / genética Marcadores Genéticos Repetições de Microssatélites |
title_short |
Extreme geographical fixation of variation in the Plasmodium falciparum gamete surface protein gene Pfs48/45 compared with microsatellite loci |
title_full |
Extreme geographical fixation of variation in the Plasmodium falciparum gamete surface protein gene Pfs48/45 compared with microsatellite loci |
title_fullStr |
Extreme geographical fixation of variation in the Plasmodium falciparum gamete surface protein gene Pfs48/45 compared with microsatellite loci |
title_full_unstemmed |
Extreme geographical fixation of variation in the Plasmodium falciparum gamete surface protein gene Pfs48/45 compared with microsatellite loci |
title_sort |
Extreme geographical fixation of variation in the Plasmodium falciparum gamete surface protein gene Pfs48/45 compared with microsatellite loci |
author |
Conway, David J |
author_facet |
Conway, David J Machado, Ricardo Luiz Dantas Singh, Balbir Dessert, Patricia Mikes, Zsuzsanna S Póvoa, Marinete Marins Oduola, Ayoade M. J Roper, Cally |
author_role |
author |
author2 |
Machado, Ricardo Luiz Dantas Singh, Balbir Dessert, Patricia Mikes, Zsuzsanna S Póvoa, Marinete Marins Oduola, Ayoade M. J Roper, Cally |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Conway, David J Machado, Ricardo Luiz Dantas Singh, Balbir Dessert, Patricia Mikes, Zsuzsanna S Póvoa, Marinete Marins Oduola, Ayoade M. J Roper, Cally |
dc.subject.decsPrimary.pt_BR.fl_str_mv |
Malária Plasmodium falciparum / parasitologia Genoma / genética Marcadores Genéticos Repetições de Microssatélites |
topic |
Malária Plasmodium falciparum / parasitologia Genoma / genética Marcadores Genéticos Repetições de Microssatélites |
description |
Comparing patterns of genetic variation at multiple loci in the genome of a species can potentially identify loci which are under selection. The large number of polymorphic microsatellites in the malaria parasite Plasmodium falciparum are available markers to screen for selectively important loci. The Pfs48/45 gene on Chromosome 13 encodes an antigenic protein located on the surface of parasite gametes, which is a candidate for a transmission blocking vaccine. Here, genotypic data from 255 P. falciparum isolates are presented, which show that alleles and haplotypes of five single nucleotide polymorphisms (SNPs) in the Pfs48/45 gene are exceptionally skewed in frequency among different P. falciparum populations, compared with alleles at 11 microsatellite loci sampled widely from the parasite genome. Fixation indices measuring inter-population variance in allele frequencies (FST) were in the order of four to seven times higher for Pfs48/45 than for the microsatellites, whether considered (i) among populations within Africa, or (ii) among different continents. Differing mutational processes at microsatellite and SNP loci could generally affect the population structure at these different types of loci, to an unknown extent which deserves further investigation. The highly contrasting population structure may also suggest divergent selection on the amino acid sequence of Pfs48/45 in different populations, which plausibly indicates a role for the protein in determining gamete recognition and compatibility. © 2001 Elsevier Science B.V. |
publishDate |
2001 |
dc.date.issued.fl_str_mv |
2001 |
dc.date.accessioned.fl_str_mv |
2021-07-19T22:28:43Z |
dc.date.available.fl_str_mv |
2021-07-19T22:28:43Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
CONWAY, David J. et al. Extreme geographical fixation of variation in the Plasmodium falciparum gamete surface protein gene Pfs48/45 compared with microsatellite loci. Molecular and Biochemical Parasitology, v. 115, n. 2, p. 145-156, July 2001. |
dc.identifier.uri.fl_str_mv |
https://patua.iec.gov.br/handle/iec/4362 |
dc.identifier.issn.-.fl_str_mv |
0166-6851 |
dc.identifier.doi.-.fl_str_mv |
10.1016/s0166-6851(01)00278-x. |
identifier_str_mv |
CONWAY, David J. et al. Extreme geographical fixation of variation in the Plasmodium falciparum gamete surface protein gene Pfs48/45 compared with microsatellite loci. Molecular and Biochemical Parasitology, v. 115, n. 2, p. 145-156, July 2001. 0166-6851 10.1016/s0166-6851(01)00278-x. |
url |
https://patua.iec.gov.br/handle/iec/4362 |
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eng |
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eng |
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info:eu-repo/semantics/openAccess |
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openAccess |
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Elsevier |
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Elsevier |
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