Virulence associated factors in bacteria from water bodies in Belem, Para, Brazil: bacteriological composition and threat to public health

• Autor(a) principal: Trindade, Lucas Monteiro da
• Data de Publicação: 2021
• Outros Autores: Correa Filho, Luiz Claudio Gomes, Santos, Denise Suellen Amorim de Sousa, Sousa, Nayara Rufino, Vale, Elivam Rodrigues, Rocha, Daniela Cristiane da Cruz, Morais, Lena Líllian Canto de Sá
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Digital do Instituto Evandro Chagas (Patuá)
• Texto Completo: https://patua.iec.gov.br/handle/iec/4307
• topic: Águas Superficiais; Águas Residuárias / virologia; Poluição da Água / análise; Saneamento Básico; Esgotos / virologia; Coliformes; Gastroenterite / virologia; Reação em Cadeia da Polimerase / métodos; Ecossistema Amazônico

A lack of sewage treatment contaminates water bodies threatening human health by spreading waterborne gastroenteritis. This is a particular problem for developing countries, where the risks associated with surface water contamination remain largely unknown. To understand the risk associated with sewage contamination of water bodies, we evaluated the microbiological indicators of water quality and isolated bacterial strains from water bodies from the city of Belém, Pará, Brazil. The strains were identified by biochemical and serological tests and polymerase chain reactions (PCRs). The thermotolerant coliforms and Escherichia coli presented values above 1,000 (NMP/ 100 mL) biweekly from August 2012 to November 2015, without a significant statistical difference between sampling periods (Kruskal–Wallis p > 0.05). The water of the Tucunduba river presented contamination levels similar to those in a sewage pumping station (Dunn test p > 0.05). From 240 bacterial isolates, we identified 163 Vibrio cholerae, 8 Vibrio mimicus, 24 E. coli, and 5 Salmonella spp. The isolates of V. cholerae demonstrated N-acetylglucosamine (NAG) profile (Non-O1 and NonO139) and 18 expressed the stn/sto gene. No E. coli was shown to be potentially pathogenic. The results revealed that water bodies in Belém were constantly contaminated by sewage and fecal microorganisms, including the potential circulation of pathogens in viable and cultivable form.