Virulence associated factors in bacteria from water bodies in Belem, Para, Brazil: bacteriological composition and threat to public health

Detalhes bibliográficos
Autor(a) principal: Trindade, Lucas Monteiro da
Data de Publicação: 2021
Outros Autores: Correa Filho, Luiz Claudio Gomes, Santos, Denise Suellen Amorim de Sousa, Sousa, Nayara Rufino, Vale, Elivam Rodrigues, Rocha, Daniela Cristiane da Cruz, Morais, Lena Líllian Canto de Sá
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Digital do Instituto Evandro Chagas (Patuá)
Texto Completo: https://patua.iec.gov.br/handle/iec/4307
Resumo: A lack of sewage treatment contaminates water bodies threatening human health by spreading waterborne gastroenteritis. This is a particular problem for developing countries, where the risks associated with surface water contamination remain largely unknown. To understand the risk associated with sewage contamination of water bodies, we evaluated the microbiological indicators of water quality and isolated bacterial strains from water bodies from the city of Belém, Pará, Brazil. The strains were identified by biochemical and serological tests and polymerase chain reactions (PCRs). The thermotolerant coliforms and Escherichia coli presented values above 1,000 (NMP/ 100 mL) biweekly from August 2012 to November 2015, without a significant statistical difference between sampling periods (Kruskal–Wallis p > 0.05). The water of the Tucunduba river presented contamination levels similar to those in a sewage pumping station (Dunn test p > 0.05). From 240 bacterial isolates, we identified 163 Vibrio cholerae, 8 Vibrio mimicus, 24 E. coli, and 5 Salmonella spp. The isolates of V. cholerae demonstrated N-acetylglucosamine (NAG) profile (Non-O1 and NonO139) and 18 expressed the stn/sto gene. No E. coli was shown to be potentially pathogenic. The results revealed that water bodies in Belém were constantly contaminated by sewage and fecal microorganisms, including the potential circulation of pathogens in viable and cultivable form.
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spelling Trindade, Lucas Monteiro daCorrea Filho, Luiz Claudio GomesSantos, Denise Suellen Amorim de SousaSousa, Nayara RufinoVale, Elivam RodriguesRocha, Daniela Cristiane da CruzMorais, Lena Líllian Canto de Sá2021-05-13T14:48:00Z2021-05-13T14:48:00Z2021TRINDADE, Lucas Monteiro da et al. Virulence associated factors in bacteria from water bodies in Belem, Para, Brazil: bacteriological composition and threat to public health. Journal of Water, Sanitation and Hygiene for Development, v. xx, n. xx, p. 1-10, 2021.2043-9083https://patua.iec.gov.br/handle/iec/430710.2166/washdev.2021.239A lack of sewage treatment contaminates water bodies threatening human health by spreading waterborne gastroenteritis. This is a particular problem for developing countries, where the risks associated with surface water contamination remain largely unknown. To understand the risk associated with sewage contamination of water bodies, we evaluated the microbiological indicators of water quality and isolated bacterial strains from water bodies from the city of Belém, Pará, Brazil. The strains were identified by biochemical and serological tests and polymerase chain reactions (PCRs). The thermotolerant coliforms and Escherichia coli presented values above 1,000 (NMP/ 100 mL) biweekly from August 2012 to November 2015, without a significant statistical difference between sampling periods (Kruskal–Wallis p > 0.05). The water of the Tucunduba river presented contamination levels similar to those in a sewage pumping station (Dunn test p > 0.05). From 240 bacterial isolates, we identified 163 Vibrio cholerae, 8 Vibrio mimicus, 24 E. coli, and 5 Salmonella spp. The isolates of V. cholerae demonstrated N-acetylglucosamine (NAG) profile (Non-O1 and NonO139) and 18 expressed the stn/sto gene. No E. coli was shown to be potentially pathogenic. The results revealed that water bodies in Belém were constantly contaminated by sewage and fecal microorganisms, including the potential circulation of pathogens in viable and cultivable form.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Laboratório de Microbiologia Ambiental. Ananindeua, PA, Brasil.Federal University of Pará. Belém, PA, Brazil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Laboratório de Microbiologia Ambiental. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Laboratório de Microbiologia Ambiental. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Laboratório de Microbiologia Ambiental. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Laboratório de Biologia Molecular. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Laboratório de Microbiologia Ambiental. 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dc.title.pt_BR.fl_str_mv Virulence associated factors in bacteria from water bodies in Belem, Para, Brazil: bacteriological composition and threat to public health
title Virulence associated factors in bacteria from water bodies in Belem, Para, Brazil: bacteriological composition and threat to public health
spellingShingle Virulence associated factors in bacteria from water bodies in Belem, Para, Brazil: bacteriological composition and threat to public health
Trindade, Lucas Monteiro da
Águas Superficiais
Águas Residuárias / virologia
Poluição da Água / análise
Saneamento Básico
Esgotos / virologia
Coliformes
Gastroenterite / virologia
Reação em Cadeia da Polimerase / métodos
Ecossistema Amazônico
title_short Virulence associated factors in bacteria from water bodies in Belem, Para, Brazil: bacteriological composition and threat to public health
title_full Virulence associated factors in bacteria from water bodies in Belem, Para, Brazil: bacteriological composition and threat to public health
title_fullStr Virulence associated factors in bacteria from water bodies in Belem, Para, Brazil: bacteriological composition and threat to public health
title_full_unstemmed Virulence associated factors in bacteria from water bodies in Belem, Para, Brazil: bacteriological composition and threat to public health
title_sort Virulence associated factors in bacteria from water bodies in Belem, Para, Brazil: bacteriological composition and threat to public health
author Trindade, Lucas Monteiro da
author_facet Trindade, Lucas Monteiro da
Correa Filho, Luiz Claudio Gomes
Santos, Denise Suellen Amorim de Sousa
Sousa, Nayara Rufino
Vale, Elivam Rodrigues
Rocha, Daniela Cristiane da Cruz
Morais, Lena Líllian Canto de Sá
author_role author
author2 Correa Filho, Luiz Claudio Gomes
Santos, Denise Suellen Amorim de Sousa
Sousa, Nayara Rufino
Vale, Elivam Rodrigues
Rocha, Daniela Cristiane da Cruz
Morais, Lena Líllian Canto de Sá
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Trindade, Lucas Monteiro da
Correa Filho, Luiz Claudio Gomes
Santos, Denise Suellen Amorim de Sousa
Sousa, Nayara Rufino
Vale, Elivam Rodrigues
Rocha, Daniela Cristiane da Cruz
Morais, Lena Líllian Canto de Sá
dc.subject.decsPrimary.pt_BR.fl_str_mv Águas Superficiais
Águas Residuárias / virologia
Poluição da Água / análise
Saneamento Básico
Esgotos / virologia
Coliformes
Gastroenterite / virologia
Reação em Cadeia da Polimerase / métodos
Ecossistema Amazônico
topic Águas Superficiais
Águas Residuárias / virologia
Poluição da Água / análise
Saneamento Básico
Esgotos / virologia
Coliformes
Gastroenterite / virologia
Reação em Cadeia da Polimerase / métodos
Ecossistema Amazônico
description A lack of sewage treatment contaminates water bodies threatening human health by spreading waterborne gastroenteritis. This is a particular problem for developing countries, where the risks associated with surface water contamination remain largely unknown. To understand the risk associated with sewage contamination of water bodies, we evaluated the microbiological indicators of water quality and isolated bacterial strains from water bodies from the city of Belém, Pará, Brazil. The strains were identified by biochemical and serological tests and polymerase chain reactions (PCRs). The thermotolerant coliforms and Escherichia coli presented values above 1,000 (NMP/ 100 mL) biweekly from August 2012 to November 2015, without a significant statistical difference between sampling periods (Kruskal–Wallis p > 0.05). The water of the Tucunduba river presented contamination levels similar to those in a sewage pumping station (Dunn test p > 0.05). From 240 bacterial isolates, we identified 163 Vibrio cholerae, 8 Vibrio mimicus, 24 E. coli, and 5 Salmonella spp. The isolates of V. cholerae demonstrated N-acetylglucosamine (NAG) profile (Non-O1 and NonO139) and 18 expressed the stn/sto gene. No E. coli was shown to be potentially pathogenic. The results revealed that water bodies in Belém were constantly contaminated by sewage and fecal microorganisms, including the potential circulation of pathogens in viable and cultivable form.
publishDate 2021
dc.date.accessioned.fl_str_mv 2021-05-13T14:48:00Z
dc.date.available.fl_str_mv 2021-05-13T14:48:00Z
dc.date.issued.fl_str_mv 2021
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv TRINDADE, Lucas Monteiro da et al. Virulence associated factors in bacteria from water bodies in Belem, Para, Brazil: bacteriological composition and threat to public health. Journal of Water, Sanitation and Hygiene for Development, v. xx, n. xx, p. 1-10, 2021.
dc.identifier.uri.fl_str_mv https://patua.iec.gov.br/handle/iec/4307
dc.identifier.issn.-.fl_str_mv 2043-9083
dc.identifier.doi.-.fl_str_mv 10.2166/washdev.2021.239
identifier_str_mv TRINDADE, Lucas Monteiro da et al. Virulence associated factors in bacteria from water bodies in Belem, Para, Brazil: bacteriological composition and threat to public health. Journal of Water, Sanitation and Hygiene for Development, v. xx, n. xx, p. 1-10, 2021.
2043-9083
10.2166/washdev.2021.239
url https://patua.iec.gov.br/handle/iec/4307
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collection Repositório Digital do Instituto Evandro Chagas (Patuá)
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