Genotype diversity and molecular evolution of noroviruses: a 30-year (1982-2011) comprehensive study with children from Northern Brazil

Detalhes bibliográficos
Autor(a) principal: Siqueira, Jones Anderson Monteiro
Data de Publicação: 2017
Outros Autores: Bandeira, Renato da Silva, Oliveira, Darleise de Souza, Santos, Liann Filiphe Pereira dos, Gabbay, Yvone Benchimol
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Digital do Instituto Evandro Chagas (Patuá)
Texto Completo: https://patua.iec.gov.br/handle/iec/2860
Resumo: A chronologically comprehensive 30-year study was conducted that involved children living in Belém, in the Amazon region of Northern Brazil, who participated in eight different studies from October 1982 to April 2011. The children were followed either in the community or in health units and hospitals in order to identify the norovirus genotypes involved in infections during this time. A total of 2,520 fecal specimens were obtained and subjected to RT-PCR and nucleotide sequencing for regions A, B, C, D and P2 of the viral genome. An overall positivity of 16.9% (n = 426) was observed, and 49% of the positive samples were genotyped (208/426), evidencing the presence of several genotypes as follows: Polymerase gene (GI.P4, GII.Pa, GII.Pc, GII.Pe, GII.Pg, GII.Pj, GII.P3, GII.P4, GII.P6, GII.P7, GII.P8, GII.P12, GII.P13, GII.P14, GII.P21, GII.P22), and VP1 gene (GI.3, GI.7, GII.1, GII.2, GII.3, GII.4, GII.6, GII.7, GII.8, GII.10, GII.12, GII.14, GII.17, GII.23). The GII.P4/GII.4 genotype determined by both open reading frames (ORFs) (partial polymerase and VP1 genes) was found for 83 samples, and analyses of the subdomain P2 region showed 10 different variants: CHDC (1970s), Tokyo (1980s), Bristol_1993, US_95/96, Kaiso_2003, Asia_2003, Hunter_2004, Yerseke_2006a, Den Haag_2006b (subcluster “O”) and New Orleans_2009. Recombination events were confirmed in 47.6% (n = 20) of the 42 samples with divergent genotyping by ORF1 and ORF2 and with probable different breakpoints within the viral genome. The evolutionary analyses estimated a rate of evolution of 1.02 x 10−2 and 9.05 x 10−3 subs./site/year using regions C and D from the VP1 gene, respectively. The present research shows the broad genetic diversity of the norovirus that infected children for 30 years in Belém. These findings contribute to our understanding of noroviruses molecular epidemiology and viral evolution and provide a baseline for vaccine design.
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spelling Siqueira, Jones Anderson MonteiroBandeira, Renato da SilvaOliveira, Darleise de SouzaSantos, Liann Filiphe Pereira dosGabbay, Yvone Benchimol2017-11-20T19:40:22Z2017-11-20T19:40:22Z2017SIQUEIRA, Jones Anderson Monteiro. Genotype diversity and molecular evolution of noroviruses: a 30-year (1982-2011) comprehensive study with children from Northern Brazil. PLoS One, v. 12, n. 6, p. e0178909, June 2017.1932-6203https://patua.iec.gov.br/handle/iec/286010.1371/journal.pone.0178909A chronologically comprehensive 30-year study was conducted that involved children living in Belém, in the Amazon region of Northern Brazil, who participated in eight different studies from October 1982 to April 2011. The children were followed either in the community or in health units and hospitals in order to identify the norovirus genotypes involved in infections during this time. A total of 2,520 fecal specimens were obtained and subjected to RT-PCR and nucleotide sequencing for regions A, B, C, D and P2 of the viral genome. An overall positivity of 16.9% (n = 426) was observed, and 49% of the positive samples were genotyped (208/426), evidencing the presence of several genotypes as follows: Polymerase gene (GI.P4, GII.Pa, GII.Pc, GII.Pe, GII.Pg, GII.Pj, GII.P3, GII.P4, GII.P6, GII.P7, GII.P8, GII.P12, GII.P13, GII.P14, GII.P21, GII.P22), and VP1 gene (GI.3, GI.7, GII.1, GII.2, GII.3, GII.4, GII.6, GII.7, GII.8, GII.10, GII.12, GII.14, GII.17, GII.23). The GII.P4/GII.4 genotype determined by both open reading frames (ORFs) (partial polymerase and VP1 genes) was found for 83 samples, and analyses of the subdomain P2 region showed 10 different variants: CHDC (1970s), Tokyo (1980s), Bristol_1993, US_95/96, Kaiso_2003, Asia_2003, Hunter_2004, Yerseke_2006a, Den Haag_2006b (subcluster “O”) and New Orleans_2009. Recombination events were confirmed in 47.6% (n = 20) of the 42 samples with divergent genotyping by ORF1 and ORF2 and with probable different breakpoints within the viral genome. The evolutionary analyses estimated a rate of evolution of 1.02 x 10−2 and 9.05 x 10−3 subs./site/year using regions C and D from the VP1 gene, respectively. The present research shows the broad genetic diversity of the norovirus that infected children for 30 years in Belém. These findings contribute to our understanding of noroviruses molecular epidemiology and viral evolution and provide a baseline for vaccine design.This research was supported by Evandro Chagas Institute and Fundação Amazônia de Amparo a Estudos e Pesquisa do Pará – Fapespa (Edital: 006/2014 – Programa de auxílio à pesquisa/Projetos temáticos da Fapespa: Saúde).Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Laboratório de Norovírus e outros Vírus Gastroentéricos. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Laboratório de Norovírus e outros Vírus Gastroentéricos. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Laboratório de Norovírus e outros Vírus Gastroentéricos. Ananindeua, PA, Brasil.engPublic Library of ScienceGenotype diversity and molecular evolution of noroviruses: a 30-year (1982-2011) comprehensive study with children from Northern Brazilinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article19822011Norovirus / genéticaNorovirus / isolamento & purificaçãoFezes / virologiaRNA Viral / genéticaRNA Viral / isolamento & purificaçãoGenoma Viral / genéticaVariação Genética / genéticaFilogeniaGenótipoDNA Complementar / genéticaAnálise de Sequência de DNASequenciamento de Nucleotídeos em Larga EscalaRecombinação GenéticaTaxa de MutaçãoDistribuição TemporalEvolução MolecularEpidemiologia MolecularEnsaio de Imunoadsorção Enzimática / métodosReação em Cadeia da Polimerase em Tempo Real / métodosReação em Cadeia da Polimerase Via Transcriptase Reversa / métodosinfo:eu-repo/semantics/openAccessreponame:Repositório Digital do Instituto Evandro Chagas (Patuá)instname:Instituto Evandro Chagas (IEC)instacron:IECORIGINALGenotype diversity and molecular evolution of noroviruses : a 30-year (1982-2011) comprehensive study with children from Northern Brazil.pdfGenotype diversity and molecular evolution of noroviruses : a 30-year (1982-2011) comprehensive study with children from Northern Brazil.pdfapplication/pdf3528142https://patua.iec.gov.br/bitstreams/59bbecb1-7eeb-423b-b78e-636a4db97e41/downloadacbe553608125750448e8ce3733d9439MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-871https://patua.iec.gov.br/bitstreams/24624cc1-84fa-40e0-8876-847383900c21/download52f1732ea66fbd1123abe39f5373b797MD52TEXTGenotype diversity and molecular evolution of noroviruses : a 30-year (1982-2011) comprehensive study with children from Northern Brazil.pdf.txtGenotype diversity and molecular evolution of noroviruses : a 30-year (1982-2011) comprehensive study with children from Northern Brazil.pdf.txtExtracted texttext/plain81084https://patua.iec.gov.br/bitstreams/f383e05a-a63e-4f4d-8fd0-d86a00de18ae/download1b45d8ad926c0beae28f3ca3157cf711MD55THUMBNAILGenotype diversity and molecular evolution of noroviruses : a 30-year (1982-2011) comprehensive study with children from Northern Brazil.pdf.jpgGenotype diversity and molecular evolution of noroviruses : a 30-year (1982-2011) comprehensive study with children from Northern Brazil.pdf.jpgGenerated Thumbnailimage/jpeg5848https://patua.iec.gov.br/bitstreams/e5966412-059e-45cc-a3df-53f7a314109f/download96b6f4d0a90fcf475086e02be9174285MD56iec/28602022-10-20 21:14:26.778oai:patua.iec.gov.br:iec/2860https://patua.iec.gov.brRepositório InstitucionalPUBhttps://patua.iec.gov.br/oai/requestclariceneta@iec.gov.br || Biblioteca@iec.gov.bropendoar:2022-10-20T21:14:26Repositório Digital do Instituto Evandro Chagas (Patuá) - Instituto Evandro Chagas (IEC)falseVG9kb3Mgb3MgZG9jdW1lbnRvcyBkZXNzYSBjb2xlw6fDo28gc2VndWVtIGEgTGljZW7Dp2EgQ3JlYXRpdmUgY29tbW9ucy4=
dc.title.pt_BR.fl_str_mv Genotype diversity and molecular evolution of noroviruses: a 30-year (1982-2011) comprehensive study with children from Northern Brazil
title Genotype diversity and molecular evolution of noroviruses: a 30-year (1982-2011) comprehensive study with children from Northern Brazil
spellingShingle Genotype diversity and molecular evolution of noroviruses: a 30-year (1982-2011) comprehensive study with children from Northern Brazil
Siqueira, Jones Anderson Monteiro
Norovirus / genética
Norovirus / isolamento & purificação
Fezes / virologia
RNA Viral / genética
RNA Viral / isolamento & purificação
Genoma Viral / genética
Variação Genética / genética
Filogenia
Genótipo
DNA Complementar / genética
Análise de Sequência de DNA
Sequenciamento de Nucleotídeos em Larga Escala
Recombinação Genética
Taxa de Mutação
Distribuição Temporal
Evolução Molecular
Epidemiologia Molecular
Ensaio de Imunoadsorção Enzimática / métodos
Reação em Cadeia da Polimerase em Tempo Real / métodos
Reação em Cadeia da Polimerase Via Transcriptase Reversa / métodos
title_short Genotype diversity and molecular evolution of noroviruses: a 30-year (1982-2011) comprehensive study with children from Northern Brazil
title_full Genotype diversity and molecular evolution of noroviruses: a 30-year (1982-2011) comprehensive study with children from Northern Brazil
title_fullStr Genotype diversity and molecular evolution of noroviruses: a 30-year (1982-2011) comprehensive study with children from Northern Brazil
title_full_unstemmed Genotype diversity and molecular evolution of noroviruses: a 30-year (1982-2011) comprehensive study with children from Northern Brazil
title_sort Genotype diversity and molecular evolution of noroviruses: a 30-year (1982-2011) comprehensive study with children from Northern Brazil
author Siqueira, Jones Anderson Monteiro
author_facet Siqueira, Jones Anderson Monteiro
Bandeira, Renato da Silva
Oliveira, Darleise de Souza
Santos, Liann Filiphe Pereira dos
Gabbay, Yvone Benchimol
author_role author
author2 Bandeira, Renato da Silva
Oliveira, Darleise de Souza
Santos, Liann Filiphe Pereira dos
Gabbay, Yvone Benchimol
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Siqueira, Jones Anderson Monteiro
Bandeira, Renato da Silva
Oliveira, Darleise de Souza
Santos, Liann Filiphe Pereira dos
Gabbay, Yvone Benchimol
dc.subject.decsPrimary.pt_BR.fl_str_mv Norovirus / genética
Norovirus / isolamento & purificação
Fezes / virologia
RNA Viral / genética
RNA Viral / isolamento & purificação
Genoma Viral / genética
Variação Genética / genética
Filogenia
Genótipo
DNA Complementar / genética
Análise de Sequência de DNA
Sequenciamento de Nucleotídeos em Larga Escala
Recombinação Genética
Taxa de Mutação
Distribuição Temporal
Evolução Molecular
Epidemiologia Molecular
Ensaio de Imunoadsorção Enzimática / métodos
Reação em Cadeia da Polimerase em Tempo Real / métodos
Reação em Cadeia da Polimerase Via Transcriptase Reversa / métodos
topic Norovirus / genética
Norovirus / isolamento & purificação
Fezes / virologia
RNA Viral / genética
RNA Viral / isolamento & purificação
Genoma Viral / genética
Variação Genética / genética
Filogenia
Genótipo
DNA Complementar / genética
Análise de Sequência de DNA
Sequenciamento de Nucleotídeos em Larga Escala
Recombinação Genética
Taxa de Mutação
Distribuição Temporal
Evolução Molecular
Epidemiologia Molecular
Ensaio de Imunoadsorção Enzimática / métodos
Reação em Cadeia da Polimerase em Tempo Real / métodos
Reação em Cadeia da Polimerase Via Transcriptase Reversa / métodos
description A chronologically comprehensive 30-year study was conducted that involved children living in Belém, in the Amazon region of Northern Brazil, who participated in eight different studies from October 1982 to April 2011. The children were followed either in the community or in health units and hospitals in order to identify the norovirus genotypes involved in infections during this time. A total of 2,520 fecal specimens were obtained and subjected to RT-PCR and nucleotide sequencing for regions A, B, C, D and P2 of the viral genome. An overall positivity of 16.9% (n = 426) was observed, and 49% of the positive samples were genotyped (208/426), evidencing the presence of several genotypes as follows: Polymerase gene (GI.P4, GII.Pa, GII.Pc, GII.Pe, GII.Pg, GII.Pj, GII.P3, GII.P4, GII.P6, GII.P7, GII.P8, GII.P12, GII.P13, GII.P14, GII.P21, GII.P22), and VP1 gene (GI.3, GI.7, GII.1, GII.2, GII.3, GII.4, GII.6, GII.7, GII.8, GII.10, GII.12, GII.14, GII.17, GII.23). The GII.P4/GII.4 genotype determined by both open reading frames (ORFs) (partial polymerase and VP1 genes) was found for 83 samples, and analyses of the subdomain P2 region showed 10 different variants: CHDC (1970s), Tokyo (1980s), Bristol_1993, US_95/96, Kaiso_2003, Asia_2003, Hunter_2004, Yerseke_2006a, Den Haag_2006b (subcluster “O”) and New Orleans_2009. Recombination events were confirmed in 47.6% (n = 20) of the 42 samples with divergent genotyping by ORF1 and ORF2 and with probable different breakpoints within the viral genome. The evolutionary analyses estimated a rate of evolution of 1.02 x 10−2 and 9.05 x 10−3 subs./site/year using regions C and D from the VP1 gene, respectively. The present research shows the broad genetic diversity of the norovirus that infected children for 30 years in Belém. These findings contribute to our understanding of noroviruses molecular epidemiology and viral evolution and provide a baseline for vaccine design.
publishDate 2017
dc.date.accessioned.fl_str_mv 2017-11-20T19:40:22Z
dc.date.available.fl_str_mv 2017-11-20T19:40:22Z
dc.date.issued.fl_str_mv 2017
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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dc.identifier.citation.fl_str_mv SIQUEIRA, Jones Anderson Monteiro. Genotype diversity and molecular evolution of noroviruses: a 30-year (1982-2011) comprehensive study with children from Northern Brazil. PLoS One, v. 12, n. 6, p. e0178909, June 2017.
dc.identifier.uri.fl_str_mv https://patua.iec.gov.br/handle/iec/2860
dc.identifier.issn.-.fl_str_mv 1932-6203
dc.identifier.doi.-.fl_str_mv 10.1371/journal.pone.0178909
identifier_str_mv SIQUEIRA, Jones Anderson Monteiro. Genotype diversity and molecular evolution of noroviruses: a 30-year (1982-2011) comprehensive study with children from Northern Brazil. PLoS One, v. 12, n. 6, p. e0178909, June 2017.
1932-6203
10.1371/journal.pone.0178909
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dc.coverage.temporalragefrom.-.fl_str_mv 1982
dc.coverage.temporalrageupto.-.fl_str_mv 2011
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