Characterization of novel intragenotype recombination events among norovirus pandemic GII.4 variants
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Digital do Instituto Evandro Chagas (Patuá) |
Texto Completo: | https://patua.iec.gov.br/handle/iec/3146 |
Resumo: | Recently, there has been an increase in the number of children hospitalized due to norovirus infection in Brazil. This is due both to the occurrence of more severe norovirus-related gastroenteritis cases after the introduction of the rotavirus vaccine and an increase in the tools for the detection of the disease. This pathogen is transmitted by the fecal-oral route, and the illness is characterized by diarrhea, vomiting, nausea and abdominal cramps. The genome of the virus is organized into three open reading frames showing strong mutation rates. Additionally, homologous recombination events, which can increase the virulence of the virus and lead to genotyping mistakes in molecular epidemiological studies, frequently occur. The purpose of this study was to describe two recombination events among different GII.4 variants that infected children who were hospitalized for severe acute gastroenteritis during distinct periods of time in Belém, Brazil. The recombination among the variants US95_96/Kaiso_2003 and Den Haag_2006b/Yerseke_2006a were observed in May 2003 and February 2009, respectively. In both cases, the association between the dominant variant at that point in time and another that was circulating at a low frequency in the population of Belém was demonstrated. Interestingly, the position of the breakpoint of the recombination event in the genome was the polymerase gene and was located at the nucleotide positions 4.834 and 5.002, which is an unusual location for the occurrence of recombination as other studies have previously reported the junction region as a breakpoint. In this study, both recombinant variant strains were related to severe cases of diarrhea that lead to hospitalization, demonstrating the viral evolution of GII.4 in response to selective pressures, which ultimately lead to the emergence of novel viral types in the pediatric population. The cases discussed here reinforce the need for continuous norovirus surveillance. To our knowledge, these two GII.4 variant recombinations have not yet been previously described. |
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Siqueira, Jones Anderson MonteiroBandeira, Renato da SilvaJustino, Maria Cleonice AguiarLinhares, Alexandre da CostaGabbay, Yvone Benchimol2018-04-27T17:38:21Z2018-04-27T17:38:21Z2016SIQUEIRA, Jones Anderson Monteiro et al. Characterization of novel intragenotype recombination events among norovirus pandemic GII.4 variants. Infection, Genetics, Evolution, v. 44, p. 361-366, Oct. 2016.1567-1348https://patua.iec.gov.br/handle/iec/314610.1016/j.meegid.2016.07.037Recently, there has been an increase in the number of children hospitalized due to norovirus infection in Brazil. This is due both to the occurrence of more severe norovirus-related gastroenteritis cases after the introduction of the rotavirus vaccine and an increase in the tools for the detection of the disease. This pathogen is transmitted by the fecal-oral route, and the illness is characterized by diarrhea, vomiting, nausea and abdominal cramps. The genome of the virus is organized into three open reading frames showing strong mutation rates. Additionally, homologous recombination events, which can increase the virulence of the virus and lead to genotyping mistakes in molecular epidemiological studies, frequently occur. The purpose of this study was to describe two recombination events among different GII.4 variants that infected children who were hospitalized for severe acute gastroenteritis during distinct periods of time in Belém, Brazil. The recombination among the variants US95_96/Kaiso_2003 and Den Haag_2006b/Yerseke_2006a were observed in May 2003 and February 2009, respectively. In both cases, the association between the dominant variant at that point in time and another that was circulating at a low frequency in the population of Belém was demonstrated. Interestingly, the position of the breakpoint of the recombination event in the genome was the polymerase gene and was located at the nucleotide positions 4.834 and 5.002, which is an unusual location for the occurrence of recombination as other studies have previously reported the junction region as a breakpoint. In this study, both recombinant variant strains were related to severe cases of diarrhea that lead to hospitalization, demonstrating the viral evolution of GII.4 in response to selective pressures, which ultimately lead to the emergence of novel viral types in the pediatric population. The cases discussed here reinforce the need for continuous norovirus surveillance. To our knowledge, these two GII.4 variant recombinations have not yet been previously described.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Programa de Pós-Graduação em Virologia. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Programa de Pós-Graduação em Virologia. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.engElsevierCharacterization of novel intragenotype recombination events among norovirus pandemic GII.4 variantsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleNorovirus / genéticaRecombinação GenéticaVariação GenéticaGenoma Viral / genéticaGenótipoEstudos ProspectivosReação em Cadeia da Polimerase Via Transcriptase Reversa / métodosEcossistema Amazônicoinfo:eu-repo/semantics/embargoedAccessreponame:Repositório Digital do Instituto Evandro Chagas (Patuá)instname:Instituto Evandro Chagas (IEC)instacron:IECORIGINALCharacterization of novel intragenotype recombination events among norovirus pandemic GII.4 variants.pdfCharacterization of novel intragenotype recombination events among norovirus pandemic GII.4 variants.pdfapplication/pdf88624https://patua.iec.gov.br/bitstreams/9ac6f737-fe08-4d19-ad73-0ecb7bc0d9e9/download01aad53496e6334c1bdb327ce507a42aMD51LICENSElicense.txtlicense.txttext/plain; charset=utf-871https://patua.iec.gov.br/bitstreams/de75c461-45ba-4060-9a82-49eb2a197929/download52f1732ea66fbd1123abe39f5373b797MD52TEXTCharacterization of novel intragenotype recombination events among norovirus pandemic GII.4 variants.pdf.txtCharacterization of novel intragenotype recombination events among norovirus pandemic GII.4 variants.pdf.txtExtracted texttext/plain3422https://patua.iec.gov.br/bitstreams/ef0c55e1-2bdb-497b-ab68-c1c6f248addb/download2c20e4bc55271d7c8a126652b85e6621MD55THUMBNAILCharacterization of novel intragenotype recombination events among norovirus pandemic GII.4 variants.pdf.jpgCharacterization of novel intragenotype recombination events among norovirus pandemic GII.4 variants.pdf.jpgGenerated Thumbnailimage/jpeg6314https://patua.iec.gov.br/bitstreams/305d6350-75f1-4bd5-aa63-8f7e47542b4c/downloadab739dccbdcb10a6cc29679666402e46MD56iec/31462022-10-20 21:03:45.167oai:patua.iec.gov.br:iec/3146https://patua.iec.gov.brRepositório InstitucionalPUBhttps://patua.iec.gov.br/oai/requestclariceneta@iec.gov.br || Biblioteca@iec.gov.bropendoar:2022-10-20T21:03:45Repositório Digital do Instituto Evandro Chagas (Patuá) - Instituto Evandro Chagas (IEC)falseVG9kb3Mgb3MgZG9jdW1lbnRvcyBkZXNzYSBjb2xlw6fDo28gc2VndWVtIGEgTGljZW7Dp2EgQ3JlYXRpdmUgY29tbW9ucy4= |
dc.title.pt_BR.fl_str_mv |
Characterization of novel intragenotype recombination events among norovirus pandemic GII.4 variants |
title |
Characterization of novel intragenotype recombination events among norovirus pandemic GII.4 variants |
spellingShingle |
Characterization of novel intragenotype recombination events among norovirus pandemic GII.4 variants Siqueira, Jones Anderson Monteiro Norovirus / genética Recombinação Genética Variação Genética Genoma Viral / genética Genótipo Estudos Prospectivos Reação em Cadeia da Polimerase Via Transcriptase Reversa / métodos Ecossistema Amazônico |
title_short |
Characterization of novel intragenotype recombination events among norovirus pandemic GII.4 variants |
title_full |
Characterization of novel intragenotype recombination events among norovirus pandemic GII.4 variants |
title_fullStr |
Characterization of novel intragenotype recombination events among norovirus pandemic GII.4 variants |
title_full_unstemmed |
Characterization of novel intragenotype recombination events among norovirus pandemic GII.4 variants |
title_sort |
Characterization of novel intragenotype recombination events among norovirus pandemic GII.4 variants |
author |
Siqueira, Jones Anderson Monteiro |
author_facet |
Siqueira, Jones Anderson Monteiro Bandeira, Renato da Silva Justino, Maria Cleonice Aguiar Linhares, Alexandre da Costa Gabbay, Yvone Benchimol |
author_role |
author |
author2 |
Bandeira, Renato da Silva Justino, Maria Cleonice Aguiar Linhares, Alexandre da Costa Gabbay, Yvone Benchimol |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Siqueira, Jones Anderson Monteiro Bandeira, Renato da Silva Justino, Maria Cleonice Aguiar Linhares, Alexandre da Costa Gabbay, Yvone Benchimol |
dc.subject.decsPrimary.pt_BR.fl_str_mv |
Norovirus / genética Recombinação Genética Variação Genética Genoma Viral / genética Genótipo Estudos Prospectivos Reação em Cadeia da Polimerase Via Transcriptase Reversa / métodos Ecossistema Amazônico |
topic |
Norovirus / genética Recombinação Genética Variação Genética Genoma Viral / genética Genótipo Estudos Prospectivos Reação em Cadeia da Polimerase Via Transcriptase Reversa / métodos Ecossistema Amazônico |
description |
Recently, there has been an increase in the number of children hospitalized due to norovirus infection in Brazil. This is due both to the occurrence of more severe norovirus-related gastroenteritis cases after the introduction of the rotavirus vaccine and an increase in the tools for the detection of the disease. This pathogen is transmitted by the fecal-oral route, and the illness is characterized by diarrhea, vomiting, nausea and abdominal cramps. The genome of the virus is organized into three open reading frames showing strong mutation rates. Additionally, homologous recombination events, which can increase the virulence of the virus and lead to genotyping mistakes in molecular epidemiological studies, frequently occur. The purpose of this study was to describe two recombination events among different GII.4 variants that infected children who were hospitalized for severe acute gastroenteritis during distinct periods of time in Belém, Brazil. The recombination among the variants US95_96/Kaiso_2003 and Den Haag_2006b/Yerseke_2006a were observed in May 2003 and February 2009, respectively. In both cases, the association between the dominant variant at that point in time and another that was circulating at a low frequency in the population of Belém was demonstrated. Interestingly, the position of the breakpoint of the recombination event in the genome was the polymerase gene and was located at the nucleotide positions 4.834 and 5.002, which is an unusual location for the occurrence of recombination as other studies have previously reported the junction region as a breakpoint. In this study, both recombinant variant strains were related to severe cases of diarrhea that lead to hospitalization, demonstrating the viral evolution of GII.4 in response to selective pressures, which ultimately lead to the emergence of novel viral types in the pediatric population. The cases discussed here reinforce the need for continuous norovirus surveillance. To our knowledge, these two GII.4 variant recombinations have not yet been previously described. |
publishDate |
2016 |
dc.date.issued.fl_str_mv |
2016 |
dc.date.accessioned.fl_str_mv |
2018-04-27T17:38:21Z |
dc.date.available.fl_str_mv |
2018-04-27T17:38:21Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
SIQUEIRA, Jones Anderson Monteiro et al. Characterization of novel intragenotype recombination events among norovirus pandemic GII.4 variants. Infection, Genetics, Evolution, v. 44, p. 361-366, Oct. 2016. |
dc.identifier.uri.fl_str_mv |
https://patua.iec.gov.br/handle/iec/3146 |
dc.identifier.issn.-.fl_str_mv |
1567-1348 |
dc.identifier.doi.-.fl_str_mv |
10.1016/j.meegid.2016.07.037 |
identifier_str_mv |
SIQUEIRA, Jones Anderson Monteiro et al. Characterization of novel intragenotype recombination events among norovirus pandemic GII.4 variants. Infection, Genetics, Evolution, v. 44, p. 361-366, Oct. 2016. 1567-1348 10.1016/j.meegid.2016.07.037 |
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https://patua.iec.gov.br/handle/iec/3146 |
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eng |
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info:eu-repo/semantics/embargoedAccess |
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dc.publisher.none.fl_str_mv |
Elsevier |
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Elsevier |
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