Evaluation of two molecular methods for the detection of Yellow fever virus genome

Detalhes bibliográficos
Autor(a) principal: Nunes, Márcio Roberto Teixeira
Data de Publicação: 2011
Outros Autores: Palacios, Gustavo, Nunes, Keley N. B, Casseb, Samir Mansour Moraes, Martins, Lívia Caricio, Quaresma, Juarez Antônio Simões, Savji, Nazir, Lipkin, W. Ian, Vasconcelos, Pedro Fernando da Costa
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Digital do Instituto Evandro Chagas (Patuá)
Texto Completo: https://patua.iec.gov.br/handle/iec/2734
Resumo: Yellow fever virus (YFV), a member of the family Flaviviridae, genus Flavivirus is endemic to tropical areas of Africa and South America and is among the arboviruses that pose a threat to public health. Recent outbreaks in Brazil, Bolivia, and Paraguay and the observation that vectors capable of transmitting YFV are presenting in urban areas underscore the urgency of improving surveillance and diagnostic methods. Two novel methods (RT-hemi-nested-PCR and SYBR(«) Green qRT-PCR) for efficient detection of YFV strains circulating in South America have been developed. The methods were validated using samples obtained from golden hamsters infected experimentally with wild-type YFV strains as well as human serum and tissue samples with acute disease.
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spelling Nunes, Márcio Roberto TeixeiraPalacios, GustavoNunes, Keley N. BCasseb, Samir Mansour MoraesMartins, Lívia CaricioQuaresma, Juarez Antônio SimõesSavji, NazirLipkin, W. IanVasconcelos, Pedro Fernando da Costa2017-09-13T09:01:35Z2017-09-13T09:01:35Z2011NUNES, Márcio Roberto Teixeira et al. Evaluation of two molecular methods for the detection of Yellow fever virus genome. Journal of Virological Methods, v. 174, n. 1, p. 29-34, June 2011.1879-0984https://patua.iec.gov.br/handle/iec/273410.1016/j.jviromet.2011.02.025Yellow fever virus (YFV), a member of the family Flaviviridae, genus Flavivirus is endemic to tropical areas of Africa and South America and is among the arboviruses that pose a threat to public health. Recent outbreaks in Brazil, Bolivia, and Paraguay and the observation that vectors capable of transmitting YFV are presenting in urban areas underscore the urgency of improving surveillance and diagnostic methods. Two novel methods (RT-hemi-nested-PCR and SYBR(«) Green qRT-PCR) for efficient detection of YFV strains circulating in South America have been developed. The methods were validated using samples obtained from golden hamsters infected experimentally with wild-type YFV strains as well as human serum and tissue samples with acute disease.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Columbia University. Mailman School of Public Health. Center for Infection and Immunity. New York, NY, USA.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil / Pará State University. Department of Pathology. Belém, PA, Brazil.Columbia University. Mailman School of Public Health. Center for Infection and Immunity. New York, NY, USA.Columbia University. Mailman School of Public Health. Center for Infection and Immunity. New York, NY, USA.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil / Pará State University. Department of Pathology. Belém, PA, Brazil.engElsevierEvaluation of two molecular methods for the detection of Yellow fever virus genomeinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleVírus da Febre AmarelaFebre Amarela / transmissãoFebre Amarela / epidemiologiaReação em Cadeia da Polimerase Via Transcriptase Reversa / métodosVírus da Febre Amarela / isolamento & purificaçãoArbovirus / isolamento & purificaçãoSaúde PúblicaFlaviviridaeinfo:eu-repo/semantics/openAccessreponame:Repositório Digital do Instituto Evandro Chagas (Patuá)instname:Instituto Evandro Chagas (IEC)instacron:IECLICENSElicense.txtlicense.txttext/plain; charset=utf-871https://patua.iec.gov.br/bitstreams/c605a9da-11bf-4e87-a011-dd77bf9d0e88/download52f1732ea66fbd1123abe39f5373b797MD52ORIGINALEvaluation of two molecular methods for the detection of Yellow fever virus genome.pdfEvaluation of two molecular methods for the detection of Yellow fever virus genome.pdfapplication/pdf617050https://patua.iec.gov.br/bitstreams/ec2e8bfd-a6f8-4290-89d8-1569ad48936b/download7ed53c8aa8858413f18a0255e90afaaaMD53TEXTEvaluation of two molecular methods for the detection of Yellow fever virus genome.pdf.txtEvaluation of two molecular methods for the detection of Yellow fever virus genome.pdf.txtExtracted texttext/plain34085https://patua.iec.gov.br/bitstreams/c6f35e7b-d9d7-49ae-8be5-671e9223f8f5/download30b6ea0eb7c71bf49ada15fcb419d8bfMD56THUMBNAILEvaluation of two molecular methods for the detection of Yellow fever virus genome.pdf.jpgEvaluation of two molecular methods for the detection of Yellow fever virus genome.pdf.jpgGenerated Thumbnailimage/jpeg5516https://patua.iec.gov.br/bitstreams/c5183a11-1a6c-4f05-b3e3-bb26b447b8e9/downloadbf125e32e9b09214aa5de06d6e00f6acMD57iec/27342022-10-21 00:25:14.81oai:patua.iec.gov.br:iec/2734https://patua.iec.gov.brRepositório InstitucionalPUBhttps://patua.iec.gov.br/oai/requestclariceneta@iec.gov.br || Biblioteca@iec.gov.bropendoar:2022-10-21T00:25:14Repositório Digital do Instituto Evandro Chagas (Patuá) - Instituto Evandro Chagas (IEC)falseVG9kb3Mgb3MgZG9jdW1lbnRvcyBkZXNzYSBjb2xlw6fDo28gc2VndWVtIGEgTGljZW7Dp2EgQ3JlYXRpdmUgY29tbW9ucy4=
dc.title.pt_BR.fl_str_mv Evaluation of two molecular methods for the detection of Yellow fever virus genome
title Evaluation of two molecular methods for the detection of Yellow fever virus genome
spellingShingle Evaluation of two molecular methods for the detection of Yellow fever virus genome
Nunes, Márcio Roberto Teixeira
Vírus da Febre Amarela
Febre Amarela / transmissão
Febre Amarela / epidemiologia
Reação em Cadeia da Polimerase Via Transcriptase Reversa / métodos
Vírus da Febre Amarela / isolamento & purificação
Arbovirus / isolamento & purificação
Saúde Pública
Flaviviridae
title_short Evaluation of two molecular methods for the detection of Yellow fever virus genome
title_full Evaluation of two molecular methods for the detection of Yellow fever virus genome
title_fullStr Evaluation of two molecular methods for the detection of Yellow fever virus genome
title_full_unstemmed Evaluation of two molecular methods for the detection of Yellow fever virus genome
title_sort Evaluation of two molecular methods for the detection of Yellow fever virus genome
author Nunes, Márcio Roberto Teixeira
author_facet Nunes, Márcio Roberto Teixeira
Palacios, Gustavo
Nunes, Keley N. B
Casseb, Samir Mansour Moraes
Martins, Lívia Caricio
Quaresma, Juarez Antônio Simões
Savji, Nazir
Lipkin, W. Ian
Vasconcelos, Pedro Fernando da Costa
author_role author
author2 Palacios, Gustavo
Nunes, Keley N. B
Casseb, Samir Mansour Moraes
Martins, Lívia Caricio
Quaresma, Juarez Antônio Simões
Savji, Nazir
Lipkin, W. Ian
Vasconcelos, Pedro Fernando da Costa
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Nunes, Márcio Roberto Teixeira
Palacios, Gustavo
Nunes, Keley N. B
Casseb, Samir Mansour Moraes
Martins, Lívia Caricio
Quaresma, Juarez Antônio Simões
Savji, Nazir
Lipkin, W. Ian
Vasconcelos, Pedro Fernando da Costa
dc.subject.decsPrimary.pt_BR.fl_str_mv Vírus da Febre Amarela
Febre Amarela / transmissão
Febre Amarela / epidemiologia
Reação em Cadeia da Polimerase Via Transcriptase Reversa / métodos
Vírus da Febre Amarela / isolamento & purificação
Arbovirus / isolamento & purificação
Saúde Pública
Flaviviridae
topic Vírus da Febre Amarela
Febre Amarela / transmissão
Febre Amarela / epidemiologia
Reação em Cadeia da Polimerase Via Transcriptase Reversa / métodos
Vírus da Febre Amarela / isolamento & purificação
Arbovirus / isolamento & purificação
Saúde Pública
Flaviviridae
description Yellow fever virus (YFV), a member of the family Flaviviridae, genus Flavivirus is endemic to tropical areas of Africa and South America and is among the arboviruses that pose a threat to public health. Recent outbreaks in Brazil, Bolivia, and Paraguay and the observation that vectors capable of transmitting YFV are presenting in urban areas underscore the urgency of improving surveillance and diagnostic methods. Two novel methods (RT-hemi-nested-PCR and SYBR(«) Green qRT-PCR) for efficient detection of YFV strains circulating in South America have been developed. The methods were validated using samples obtained from golden hamsters infected experimentally with wild-type YFV strains as well as human serum and tissue samples with acute disease.
publishDate 2011
dc.date.issued.fl_str_mv 2011
dc.date.accessioned.fl_str_mv 2017-09-13T09:01:35Z
dc.date.available.fl_str_mv 2017-09-13T09:01:35Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv NUNES, Márcio Roberto Teixeira et al. Evaluation of two molecular methods for the detection of Yellow fever virus genome. Journal of Virological Methods, v. 174, n. 1, p. 29-34, June 2011.
dc.identifier.uri.fl_str_mv https://patua.iec.gov.br/handle/iec/2734
dc.identifier.issn.-.fl_str_mv 1879-0984
dc.identifier.doi.-.fl_str_mv 10.1016/j.jviromet.2011.02.025
identifier_str_mv NUNES, Márcio Roberto Teixeira et al. Evaluation of two molecular methods for the detection of Yellow fever virus genome. Journal of Virological Methods, v. 174, n. 1, p. 29-34, June 2011.
1879-0984
10.1016/j.jviromet.2011.02.025
url https://patua.iec.gov.br/handle/iec/2734
dc.language.iso.fl_str_mv eng
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dc.publisher.none.fl_str_mv Elsevier
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