Biological characterization of yellow fever viruses isolated from non-human primates in Brazil with distinct genomic landscapes

• Autor(a) principal: Furtado, Nathália Dias
• Data de Publicação: 2022
• Outros Autores: Raphael, Lidiane de Menezes, Ribeiro, Ieda Pereira, Mello, Iasmim Silva de, Fernandes, Déberli Ruiz, Gómez, Mariela Martínez, Santos, Alexandre Araújo Cunha dos, Nogueira, Mônica da Silva, Castro, Márcia Gonçalves de, Abreu, Filipe Vieira Santos de, Martins, Lívia Caricio, Vasconcelos, Pedro Fernando da Costa, Oliveira, Ricardo Lourenço de, Bonaldo, Myrna Cristina
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Digital do Instituto Evandro Chagas (Patuá)
• Texto Completo: https://patua.iec.gov.br/handle/iec/4537
• topic: Vírus da Febre Amarela / patogenicidade; Vírus da Febre Amarela / isolamento & purificação; Vírus da Febre Amarela / genética; Primatas / anatomia & histologia; Marcadores Genéticos

Since the beginning of the XXI Century, the yellow fever virus (YFV) has been cyclically spreading from the Amazon basin to Brazil’s South and Southeast regions, culminating in an unprecedented outbreak that started in 2016. In this work, we studied four YFV isolated from non-human primates obtained during outbreaks in the states of Rio Grande do Sul in 2008 (PR4408), Goiás (GO05), and Espírito Santo (ES-504) in 2017, and Rio de Janeiro (RJ 155) in 2019. These isolates have genomic differences mainly distributed in non-structural proteins. We compared the isolates’ rates of infection in mammal and mosquito cells and neurovirulence in adult mice. RJ 155 and PR4408 YFV isolates exhibited higher infectivity in mammalian cells and neurovirulence in mice. In mosquito Aag2 cells, GO05 and PR4408 displayed the lowest proliferation rates. These results suggest that RJ 155 and PR4408 YFV isolates carry some genomic markers that increase infectivity in mammal hosts. From this characterization, it is possible to contribute to discovering new molecular markers for the virulence of YFV.