Polymorphisms in Toll-Like receptors genes and their associations with immunological parameters in Plasmodium vivax malaria in the Brazil-French Guiana Border
Autor(a) principal: | |
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Data de Publicação: | 2023 |
Outros Autores: | , , , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Digital do Instituto Evandro Chagas (Patuá) |
Texto Completo: | https://patua.iec.gov.br/handle/iec/6844 |
Resumo: | Background: The innate immune response plays an important role during malaria. Toll-like receptors (TLR) are capable of recognizing pathogen molecules. We aimed to evaluate five polymorphisms in TLR-4, TLR-6, and TLR-9 genes and their association with cytokine levels and clinical parameters in malaria from the Brazil-French Guiana border. Methods: A case-control study was conducted in Amapá, Brazil. P. vivax patients and individuals not infected were evaluated. Genotyping of five SNPs was carried out by qPCR. Circulating cytokines were measured by CBA. The MSP-119 IgG antibodies were performed by ELISA. Results: An association between TLR4 A299G with parasitemia was observed. There was an increase for IFN-ɤ, TNF-ɑ, IL-6, and IL-10 in the TLR-4 A299G and T3911, TLR-6 S249P, and TLR-9 1486C/T, SNPs for the studied malarial groups. There were significant findings for the TLR-4 variants A299G and T3911, TLR-9 1237C/T, and 1486C/T. For the reactivity of MSP-119 antibodies levels, no significant results were found in malaria, and control groups. Conclusions: The profile of the immune response observed by polymorphisms in TLRs genes does not seem to be standard for all types of malaria infection around the world. This can depend on the human population and the species of Plasmodium. |
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Ramírez, Aina D. RJesus, Myrela C. S. deMenezes, Rubens A. OSantos Filho, Marcelo CGomes, Margarete S. MPimenta, Tamirys SBarbosa, Vanessa SRossit, JuliaReis, Nathalia FBrito, Simone Cristina PereiraSampaio, Marrara PereiraCassiano, Gustavo CMelo, Luciane M. StortiBaptista, Andrea R. SMachado, Ricardo L. D2023-07-10T17:03:25Z2023-07-10T17:03:25Z2023RAMÍREZ, Aina D. R. et al. Polymorphisms in Toll-Like receptors genes and their associations with immunological parameters in Plasmodium vivax malaria in the Brazil-French Guiana Border. Cytokine, v. 169, n. 156278, Sept. 2023. DOI: https://doi.org/10.1016/j.cyto.2023.1562781043-4666https://patua.iec.gov.br/handle/iec/684410.1016/j.cyto.2023.156278Background: The innate immune response plays an important role during malaria. Toll-like receptors (TLR) are capable of recognizing pathogen molecules. We aimed to evaluate five polymorphisms in TLR-4, TLR-6, and TLR-9 genes and their association with cytokine levels and clinical parameters in malaria from the Brazil-French Guiana border. Methods: A case-control study was conducted in Amapá, Brazil. P. vivax patients and individuals not infected were evaluated. Genotyping of five SNPs was carried out by qPCR. Circulating cytokines were measured by CBA. The MSP-119 IgG antibodies were performed by ELISA. Results: An association between TLR4 A299G with parasitemia was observed. There was an increase for IFN-ɤ, TNF-ɑ, IL-6, and IL-10 in the TLR-4 A299G and T3911, TLR-6 S249P, and TLR-9 1486C/T, SNPs for the studied malarial groups. There were significant findings for the TLR-4 variants A299G and T3911, TLR-9 1237C/T, and 1486C/T. For the reactivity of MSP-119 antibodies levels, no significant results were found in malaria, and control groups. Conclusions: The profile of the immune response observed by polymorphisms in TLRs genes does not seem to be standard for all types of malaria infection around the world. This can depend on the human population and the species of Plasmodium.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) finance code 001, and Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq). ADRR was supported by an OEA scholarship, and MCSDJ and MCSF were supported by a CAPES fellowship.Fluminense Federal University. Biomedical Institute. Department of Microbiology and Parasitology. Center for Microorganisms’ Investigation. Niterói, RJ, Brazil / Fluminense Federal University. Biomedical Institute. Postgraduate Program in Applied Microbiology and Parasitology. Niterói, RJ, Brazil.Fluminense Federal University. Biomedical Institute. Department of Microbiology and Parasitology. Center for Microorganisms’ Investigation. Niterói, RJ, Brazil / Fluminense Federal University. Biomedical Institute. Postgraduate Program in Applied Microbiology and Parasitology. Niterói, RJ, Brazil.Fluminense Federal University. Biomedical Institute. Postgraduate Program in Applied Microbiology and Parasitology. Niterói, RJ, Brazil / Federal University of Amapá. Postgraduate Program in Health Sciences. Macapá, AP, Brazil.Fluminense Federal University. Biomedical Institute. Department of Microbiology and Parasitology. Center for Microorganisms’ Investigation. Niterói, RJ, Brazil / Fluminense Federal University. Biomedical Institute. Postgraduate Program in Applied Microbiology and Parasitology. Niterói, RJ, Brazil.Superintendence of Health Surveillance of the State of Amapá. Macapá, AP, Brazil.Ministério da Saúde. Secretaria de Vigilância em Saúde e Ambiente. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Fluminense Federal University. Biomedical Institute. Department of Microbiology and Parasitology. Center for Microorganisms’ Investigation. Niterói, RJ, Brazil.Fluminense Federal University. Biomedical Institute. Department of Microbiology and Parasitology. Center for Microorganisms’ Investigation. Niterói, RJ, Brazil.Fluminense Federal University. Biomedical Institute. Department of Microbiology and Parasitology. Center for Microorganisms’ Investigation. Niterói, RJ, Brazil / Fluminense Federal University. Biomedical Institute. Postgraduate Program in Applied Microbiology and Parasitology. Niterói, RJ, Brazil.Fluminense Federal University. Biomedical Institute. Department of Microbiology and Parasitology. Center for Microorganisms’ Investigation. Niterói, RJ, Brazil / Fluminense Federal University. Biomedical Institute. Postgraduate Program in Applied Microbiology and Parasitology. Niterói, RJ, Brazil.Fluminense Federal University. Biomedical Institute. Department of Microbiology and Parasitology. Center for Microorganisms’ Investigation. Niterói, RJ, Brazil / Fluminense Federal University. Biomedical Institute. Postgraduate Program in Applied Microbiology and Parasitology. Niterói, RJ, Brazil.Universidade Nova de Lisboa. Global Health and Tropical Medicine.Federal University of Sergipe. Center for Biological and Health Sciences. Department of Biology. Laboratory of Molecular Genetics and Biotechnology. São Cristóvão, SE, Brazil.Fluminense Federal University. Biomedical Institute. Department of Microbiology and Parasitology. Center for Microorganisms’ Investigation. Niterói, RJ, Brazil / Fluminense Federal University. Biomedical Institute. Postgraduate Program in Applied Microbiology and Parasitology. Niterói, RJ, Brazil.Fluminense Federal University. Biomedical Institute. Department of Microbiology and Parasitology. Center for Microorganisms’ Investigation. Niterói, RJ, Brazil / Fluminense Federal University. Biomedical Institute. Postgraduate Program in Applied Microbiology and Parasitology. Niterói, RJ, Brazil.engElsevierPolymorphisms in Toll-Like receptors genes and their associations with immunological parameters in Plasmodium vivax malaria in the Brazil-French Guiana Borderinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleMalária Vivax / parasitologiaReceptores Toll-Like / imunologiaPolimorfismo Genético / imunologiaAnticorpos / análiseinfo:eu-repo/semantics/embargoedAccessreponame:Repositório Digital do Instituto Evandro Chagas (Patuá)instname:Instituto Evandro Chagas (IEC)instacron:IECORIGINALAcesso Embargado.pdfAcesso Embargado.pdfapplication/pdf551083https://patua.iec.gov.br/bitstreams/93e64d35-2fe0-40c2-a69a-6be3056a9473/downloadc9a9c128e29cac82a5d7fdf3f4e6da73MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-82182https://patua.iec.gov.br/bitstreams/60ca8aad-2968-48fb-88c8-94bcc21bdf84/download11832eea31b16df8613079d742d61793MD52TEXTAcesso Embargado.pdf.txtAcesso Embargado.pdf.txtExtracted texttext/plain2https://patua.iec.gov.br/bitstreams/d9c33c45-7512-43f2-bbe3-eddd1ededaaf/downloade1c06d85ae7b8b032bef47e42e4c08f9MD53THUMBNAILAcesso Embargado.pdf.jpgAcesso Embargado.pdf.jpgGenerated Thumbnailimage/jpeg3095https://patua.iec.gov.br/bitstreams/f043289e-2ac9-4631-b065-a054040206ca/download71859d578212107f7f8c49a4ce09d9eeMD54iec/68442023-07-10 17:31:49.888oai:patua.iec.gov.br:iec/6844https://patua.iec.gov.brRepositório InstitucionalPUBhttps://patua.iec.gov.br/oai/requestclariceneta@iec.gov.br || Biblioteca@iec.gov.bropendoar:2023-07-10T17:31:49Repositório Digital do Instituto Evandro Chagas (Patuá) - Instituto Evandro Chagas (IEC)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 |
dc.title.pt_BR.fl_str_mv |
Polymorphisms in Toll-Like receptors genes and their associations with immunological parameters in Plasmodium vivax malaria in the Brazil-French Guiana Border |
title |
Polymorphisms in Toll-Like receptors genes and their associations with immunological parameters in Plasmodium vivax malaria in the Brazil-French Guiana Border |
spellingShingle |
Polymorphisms in Toll-Like receptors genes and their associations with immunological parameters in Plasmodium vivax malaria in the Brazil-French Guiana Border Ramírez, Aina D. R Malária Vivax / parasitologia Receptores Toll-Like / imunologia Polimorfismo Genético / imunologia Anticorpos / análise |
title_short |
Polymorphisms in Toll-Like receptors genes and their associations with immunological parameters in Plasmodium vivax malaria in the Brazil-French Guiana Border |
title_full |
Polymorphisms in Toll-Like receptors genes and their associations with immunological parameters in Plasmodium vivax malaria in the Brazil-French Guiana Border |
title_fullStr |
Polymorphisms in Toll-Like receptors genes and their associations with immunological parameters in Plasmodium vivax malaria in the Brazil-French Guiana Border |
title_full_unstemmed |
Polymorphisms in Toll-Like receptors genes and their associations with immunological parameters in Plasmodium vivax malaria in the Brazil-French Guiana Border |
title_sort |
Polymorphisms in Toll-Like receptors genes and their associations with immunological parameters in Plasmodium vivax malaria in the Brazil-French Guiana Border |
author |
Ramírez, Aina D. R |
author_facet |
Ramírez, Aina D. R Jesus, Myrela C. S. de Menezes, Rubens A. O Santos Filho, Marcelo C Gomes, Margarete S. M Pimenta, Tamirys S Barbosa, Vanessa S Rossit, Julia Reis, Nathalia F Brito, Simone Cristina Pereira Sampaio, Marrara Pereira Cassiano, Gustavo C Melo, Luciane M. Storti Baptista, Andrea R. S Machado, Ricardo L. D |
author_role |
author |
author2 |
Jesus, Myrela C. S. de Menezes, Rubens A. O Santos Filho, Marcelo C Gomes, Margarete S. M Pimenta, Tamirys S Barbosa, Vanessa S Rossit, Julia Reis, Nathalia F Brito, Simone Cristina Pereira Sampaio, Marrara Pereira Cassiano, Gustavo C Melo, Luciane M. Storti Baptista, Andrea R. S Machado, Ricardo L. D |
author2_role |
author author author author author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Ramírez, Aina D. R Jesus, Myrela C. S. de Menezes, Rubens A. O Santos Filho, Marcelo C Gomes, Margarete S. M Pimenta, Tamirys S Barbosa, Vanessa S Rossit, Julia Reis, Nathalia F Brito, Simone Cristina Pereira Sampaio, Marrara Pereira Cassiano, Gustavo C Melo, Luciane M. Storti Baptista, Andrea R. S Machado, Ricardo L. D |
dc.subject.decsPrimary.pt_BR.fl_str_mv |
Malária Vivax / parasitologia Receptores Toll-Like / imunologia Polimorfismo Genético / imunologia Anticorpos / análise |
topic |
Malária Vivax / parasitologia Receptores Toll-Like / imunologia Polimorfismo Genético / imunologia Anticorpos / análise |
description |
Background: The innate immune response plays an important role during malaria. Toll-like receptors (TLR) are capable of recognizing pathogen molecules. We aimed to evaluate five polymorphisms in TLR-4, TLR-6, and TLR-9 genes and their association with cytokine levels and clinical parameters in malaria from the Brazil-French Guiana border. Methods: A case-control study was conducted in Amapá, Brazil. P. vivax patients and individuals not infected were evaluated. Genotyping of five SNPs was carried out by qPCR. Circulating cytokines were measured by CBA. The MSP-119 IgG antibodies were performed by ELISA. Results: An association between TLR4 A299G with parasitemia was observed. There was an increase for IFN-ɤ, TNF-ɑ, IL-6, and IL-10 in the TLR-4 A299G and T3911, TLR-6 S249P, and TLR-9 1486C/T, SNPs for the studied malarial groups. There were significant findings for the TLR-4 variants A299G and T3911, TLR-9 1237C/T, and 1486C/T. For the reactivity of MSP-119 antibodies levels, no significant results were found in malaria, and control groups. Conclusions: The profile of the immune response observed by polymorphisms in TLRs genes does not seem to be standard for all types of malaria infection around the world. This can depend on the human population and the species of Plasmodium. |
publishDate |
2023 |
dc.date.accessioned.fl_str_mv |
2023-07-10T17:03:25Z |
dc.date.available.fl_str_mv |
2023-07-10T17:03:25Z |
dc.date.issued.fl_str_mv |
2023 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
RAMÍREZ, Aina D. R. et al. Polymorphisms in Toll-Like receptors genes and their associations with immunological parameters in Plasmodium vivax malaria in the Brazil-French Guiana Border. Cytokine, v. 169, n. 156278, Sept. 2023. DOI: https://doi.org/10.1016/j.cyto.2023.156278 |
dc.identifier.uri.fl_str_mv |
https://patua.iec.gov.br/handle/iec/6844 |
dc.identifier.issn.-.fl_str_mv |
1043-4666 |
dc.identifier.doi.pt_BR.fl_str_mv |
10.1016/j.cyto.2023.156278 |
identifier_str_mv |
RAMÍREZ, Aina D. R. et al. Polymorphisms in Toll-Like receptors genes and their associations with immunological parameters in Plasmodium vivax malaria in the Brazil-French Guiana Border. Cytokine, v. 169, n. 156278, Sept. 2023. DOI: https://doi.org/10.1016/j.cyto.2023.156278 1043-4666 10.1016/j.cyto.2023.156278 |
url |
https://patua.iec.gov.br/handle/iec/6844 |
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eng |
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eng |
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Elsevier |
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Elsevier |
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