Molecular characterization of Babesia vogeli in dogs from Belém, northern Brazil

• Autor(a) principal: Moraes, Pablo Henrique Gonçalves
• Data de Publicação: 2015
• Outros Autores: Rufino, C. P, Baraúna, A. R. F, Reis, T, Dall'Agnol, Leonardo Teixeira, Meneses, A. M. C, Aguiar, Délia Cristina Figueira, Nunes, Márcio Roberto Teixeira, Gonçalves, Evonnildo Costa
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Digital do Instituto Evandro Chagas (Patuá)
• Texto Completo: https://patua.iec.gov.br/handle/iec/3864
• topic: Babesiose / parasitologia; Babesiose / epidemiologia; Doenças do Cão / parasitologia; Doenças do Cão / epidemiologia; Doenças Transmitidas por Carrapatos; Babesia / isolamento & purificação; Rhipicephalus sanguineus; Análise de Sequência de DNA; Reação em Cadeia da Polimerase / métodos

Babesiosis is an infectious hemolytic disease that occurs worldwide, and is caused by a protozoan of the Babesia genus (Apicomplexa). Little is known about this genus; therefore, this study conducted a molecular characterization of Babesia spp in naturally infected dogs in northern Brazil. Blood samples were collected from 172 dogs from metropolitan Belém, and screened for Babesia spp using semi-nested polymerase chain reactions. Subsequently, 18S rDNA gene fragments were amplified and sequenced. Alignments of the 27 nucleotide sequences returned fragments measuring 1023 bp in size, which did not include any polymorphic sites (100 per cent identity). This genotype was very similar to the 18S rDNA gene in B. vogeli. This study provides an important molecular characterization of the parasite responsible for canine babesiosis in naturally infected dogs in metropolitan Belém. In addition, the 18S rDNA gene in B. vogeli may represent the most common genotype occurring in South America.