Distribution of Plasmodium vivax variants (VK210, VK247 and P. vivax-like) in three endemic areas of the Amazon region of Brazil and their correlation with chloroquine treatment
Autor(a) principal: | |
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Data de Publicação: | 2000 |
Outros Autores: | |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Digital do Instituto Evandro Chagas (Patuá) |
Texto Completo: | https://patua.iec.gov.br/handle/iec/4348 |
Resumo: | The present study evaluated the glass fibre membrane (GFM)-polymerase chain reaction (PCR) - enzymelinked immunosorbent assay (ELISA) technique for genotyping the Plasmodium vivax variants, to verify the distribution of l? vivax variants (VK2 10, VK247 and Z? viva-like) in parts of Brazil and their correlation with levels of parasitaemia, previous malaria experience and clear&e-of parasitaemia linked to different treatment schedules. The samoles were taken from individuals living in Macar& Port0 Velho and Belem. all ofwhich are endemic areas of&ax malaria in the Amazon region of?Brazil. B&d samples were collected on GFMs. The gene that codes for the circumsporozoite proteins of l? vivaxvariants was amplified by PCRand the amplified fragments were hybridized to variant-specific, digoxigenin-labelled oligonucleotide probes by ELISA. The GFM-PCR-ELISA techniaue was shown to be accurate for eoidemiolonical survevs of the vivax complex. All variants were detected & all 3 areas, but only P. vivax VK2i 0 was fo&d as a sin&e agent of infection, while the other 2 occurred as mixed infections. The l? vivax-like variant was found to be associated with low parasitaemia and VK210 with the highest parasitaemia levels; none of the l? vivux variants was linked with a previous malaria experience. In all cases parasitaemia clearance was identical regarding the type of treatment and consequently it is not possible to confirm the previously reported correlation between I? vivax genotype and response to chloroquine. |
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Machado, Ricardo L. DPóvoa, Marinete Marins2021-07-13T17:20:05Z2021-07-13T17:20:05Z2000MACHADO, Ricardo L. D.; PÓVOA, Marinete Marins. Distribution of Plasmodium vivax variants (VK210, VK247 and P. vivax-like) in three endemic areas of the Amazon region of Brazil and their correlation with chloroquine treatment. Transactions of The Royal Society of Tropical Medicine and Hygiene, v. 94, n. 4, p. 377-381, July/Aug. 2000.1878-3503https://patua.iec.gov.br/handle/iec/434810.1016/S0035-9203(00)90110-XThe present study evaluated the glass fibre membrane (GFM)-polymerase chain reaction (PCR) - enzymelinked immunosorbent assay (ELISA) technique for genotyping the Plasmodium vivax variants, to verify the distribution of l? vivax variants (VK2 10, VK247 and Z? viva-like) in parts of Brazil and their correlation with levels of parasitaemia, previous malaria experience and clear&e-of parasitaemia linked to different treatment schedules. The samoles were taken from individuals living in Macar& Port0 Velho and Belem. all ofwhich are endemic areas of&ax malaria in the Amazon region of?Brazil. B&d samples were collected on GFMs. The gene that codes for the circumsporozoite proteins of l? vivaxvariants was amplified by PCRand the amplified fragments were hybridized to variant-specific, digoxigenin-labelled oligonucleotide probes by ELISA. The GFM-PCR-ELISA techniaue was shown to be accurate for eoidemiolonical survevs of the vivax complex. All variants were detected & all 3 areas, but only P. vivax VK2i 0 was fo&d as a sin&e agent of infection, while the other 2 occurred as mixed infections. The l? vivax-like variant was found to be associated with low parasitaemia and VK210 with the highest parasitaemia levels; none of the l? vivux variants was linked with a previous malaria experience. In all cases parasitaemia clearance was identical regarding the type of treatment and consequently it is not possible to confirm the previously reported correlation between I? vivax genotype and response to chloroquine.Financial support was provided by the British Council (BRAS/881/161)/CNPq (Process 9101191953), CNPq (Process 830381/95.3), PICD/ CAPES, CDC Atlanta, Wellcome Trust and the Instituto Evandro Chagas/FUNASA.Ministério da Saúde. Fundação Nacional de Saúde. Instituto Evandro Chagas. Laboratório de Malária. Belém, PA, Brasil.Ministério da Saúde. Fundação Nacional de Saúde. Instituto Evandro Chagas. Laboratório de Malária. Belém, PA, Brasil.engOxford University PressDistribution of Plasmodium vivax variants (VK210, VK247 and P. vivax-like) in three endemic areas of the Amazon region of Brazil and their correlation with chloroquine treatmentinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleMalária Vivax / parasitologiaPlasmodium vivax / patogenicidadeTécnicas de Genotipagem / métodosCloroquina / farmacologiaReação em Cadeia da Polimerase / métodosMacapá (AP)Porto Velho (RO)Belém (PA)info:eu-repo/semantics/openAccessreponame:Repositório Digital do Instituto Evandro Chagas (Patuá)instname:Instituto Evandro Chagas (IEC)instacron:IECORIGINALDistribution of Plasmodium vivax variants (VK210, VK247 and P. vivax-like) in three endemic areas of the Amazon region of Brazil and their correlation with chloroquine treatment.pdfDistribution of Plasmodium vivax variants (VK210, VK247 and P. vivax-like) in three endemic areas of the Amazon region of Brazil and their correlation with chloroquine treatment.pdfapplication/pdf693423https://patua.iec.gov.br/bitstreams/f26233bd-8b11-4bc3-bd0f-e434b986c609/download9ef47abc95d6c04de2135d7ee33e4db6MD51LICENSElicense.txtlicense.txttext/plain; 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dc.title.pt_BR.fl_str_mv |
Distribution of Plasmodium vivax variants (VK210, VK247 and P. vivax-like) in three endemic areas of the Amazon region of Brazil and their correlation with chloroquine treatment |
title |
Distribution of Plasmodium vivax variants (VK210, VK247 and P. vivax-like) in three endemic areas of the Amazon region of Brazil and their correlation with chloroquine treatment |
spellingShingle |
Distribution of Plasmodium vivax variants (VK210, VK247 and P. vivax-like) in three endemic areas of the Amazon region of Brazil and their correlation with chloroquine treatment Machado, Ricardo L. D Malária Vivax / parasitologia Plasmodium vivax / patogenicidade Técnicas de Genotipagem / métodos Cloroquina / farmacologia Reação em Cadeia da Polimerase / métodos Macapá (AP) Porto Velho (RO) Belém (PA) |
title_short |
Distribution of Plasmodium vivax variants (VK210, VK247 and P. vivax-like) in three endemic areas of the Amazon region of Brazil and their correlation with chloroquine treatment |
title_full |
Distribution of Plasmodium vivax variants (VK210, VK247 and P. vivax-like) in three endemic areas of the Amazon region of Brazil and their correlation with chloroquine treatment |
title_fullStr |
Distribution of Plasmodium vivax variants (VK210, VK247 and P. vivax-like) in three endemic areas of the Amazon region of Brazil and their correlation with chloroquine treatment |
title_full_unstemmed |
Distribution of Plasmodium vivax variants (VK210, VK247 and P. vivax-like) in three endemic areas of the Amazon region of Brazil and their correlation with chloroquine treatment |
title_sort |
Distribution of Plasmodium vivax variants (VK210, VK247 and P. vivax-like) in three endemic areas of the Amazon region of Brazil and their correlation with chloroquine treatment |
author |
Machado, Ricardo L. D |
author_facet |
Machado, Ricardo L. D Póvoa, Marinete Marins |
author_role |
author |
author2 |
Póvoa, Marinete Marins |
author2_role |
author |
dc.contributor.author.fl_str_mv |
Machado, Ricardo L. D Póvoa, Marinete Marins |
dc.subject.decsPrimary.pt_BR.fl_str_mv |
Malária Vivax / parasitologia Plasmodium vivax / patogenicidade Técnicas de Genotipagem / métodos Cloroquina / farmacologia Reação em Cadeia da Polimerase / métodos Macapá (AP) Porto Velho (RO) Belém (PA) |
topic |
Malária Vivax / parasitologia Plasmodium vivax / patogenicidade Técnicas de Genotipagem / métodos Cloroquina / farmacologia Reação em Cadeia da Polimerase / métodos Macapá (AP) Porto Velho (RO) Belém (PA) |
description |
The present study evaluated the glass fibre membrane (GFM)-polymerase chain reaction (PCR) - enzymelinked immunosorbent assay (ELISA) technique for genotyping the Plasmodium vivax variants, to verify the distribution of l? vivax variants (VK2 10, VK247 and Z? viva-like) in parts of Brazil and their correlation with levels of parasitaemia, previous malaria experience and clear&e-of parasitaemia linked to different treatment schedules. The samoles were taken from individuals living in Macar& Port0 Velho and Belem. all ofwhich are endemic areas of&ax malaria in the Amazon region of?Brazil. B&d samples were collected on GFMs. The gene that codes for the circumsporozoite proteins of l? vivaxvariants was amplified by PCRand the amplified fragments were hybridized to variant-specific, digoxigenin-labelled oligonucleotide probes by ELISA. The GFM-PCR-ELISA techniaue was shown to be accurate for eoidemiolonical survevs of the vivax complex. All variants were detected & all 3 areas, but only P. vivax VK2i 0 was fo&d as a sin&e agent of infection, while the other 2 occurred as mixed infections. The l? vivax-like variant was found to be associated with low parasitaemia and VK210 with the highest parasitaemia levels; none of the l? vivux variants was linked with a previous malaria experience. In all cases parasitaemia clearance was identical regarding the type of treatment and consequently it is not possible to confirm the previously reported correlation between I? vivax genotype and response to chloroquine. |
publishDate |
2000 |
dc.date.issued.fl_str_mv |
2000 |
dc.date.accessioned.fl_str_mv |
2021-07-13T17:20:05Z |
dc.date.available.fl_str_mv |
2021-07-13T17:20:05Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
MACHADO, Ricardo L. D.; PÓVOA, Marinete Marins. Distribution of Plasmodium vivax variants (VK210, VK247 and P. vivax-like) in three endemic areas of the Amazon region of Brazil and their correlation with chloroquine treatment. Transactions of The Royal Society of Tropical Medicine and Hygiene, v. 94, n. 4, p. 377-381, July/Aug. 2000. |
dc.identifier.uri.fl_str_mv |
https://patua.iec.gov.br/handle/iec/4348 |
dc.identifier.issn.-.fl_str_mv |
1878-3503 |
dc.identifier.doi.-.fl_str_mv |
10.1016/S0035-9203(00)90110-X |
identifier_str_mv |
MACHADO, Ricardo L. D.; PÓVOA, Marinete Marins. Distribution of Plasmodium vivax variants (VK210, VK247 and P. vivax-like) in three endemic areas of the Amazon region of Brazil and their correlation with chloroquine treatment. Transactions of The Royal Society of Tropical Medicine and Hygiene, v. 94, n. 4, p. 377-381, July/Aug. 2000. 1878-3503 10.1016/S0035-9203(00)90110-X |
url |
https://patua.iec.gov.br/handle/iec/4348 |
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eng |
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eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
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openAccess |
dc.publisher.none.fl_str_mv |
Oxford University Press |
publisher.none.fl_str_mv |
Oxford University Press |
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reponame:Repositório Digital do Instituto Evandro Chagas (Patuá) instname:Instituto Evandro Chagas (IEC) instacron:IEC |
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Repositório Digital do Instituto Evandro Chagas (Patuá) |
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