Phylogenetic analysis of probable non-human genes of group A rotaviruses isolated from children with acute gastroenteritis in Belém, Brazil
Autor(a) principal: | |
---|---|
Data de Publicação: | 2012 |
Outros Autores: | , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Digital do Instituto Evandro Chagas (Patuá) |
Texto Completo: | https://patua.iec.gov.br/handle/iec/3268 |
Resumo: | Rotaviruses (RVs) are the main cause of acute viral gastroenteritis in both humans and young animals of various species such as calves, horses, pigs, dogs, cats, and birds. The genetic diversity of RVs is related to a variety of evolutionary mechanisms, including point mutation, and genome reassortment. The objective of this study was to characterize molecularly genes that encode structural and nonstructural proteins in unusual RV strains. The clinical specimens selected for this study were obtained from children and newborn with RV gastroenteritis, who participated in research projects on viral gastroenteritis conducted at the Evandro Chagas Institute. Structural (VP1-VP4, VP6, and VP7) and nonstructural (NSP1-NSP6) genes were amplified from stool samples by the polymerase chain reaction and subsequently sequenced. Eight unusual RV strains isolated from children and newborn with gastroenteritis were studied. Reassortment between genes of animal origin were observed in 5/8 (62.5%) strains analyzed. These results demonstrate that, although rare, interspecies (animal-human) transmission of RVs occurs in nature, as observed in the present study in strains NB150, HSP034, HSP180, HST327, and RV10109. This study is the first to be conducted in the Amazon region and supports previous data showing a close relationship between genes of human and animal origin, representing a challenge to the large-scale introduction of RV vaccines in national immunization programs. |
id |
IEC-2_b2ff2d4f8c3336414abb649e44bd5a7f |
---|---|
oai_identifier_str |
oai:patua.iec.gov.br:iec/3268 |
network_acronym_str |
IEC-2 |
network_name_str |
Repositório Digital do Instituto Evandro Chagas (Patuá) |
repository_id_str |
|
spelling |
Maestri, Régis PiloniKaiano, Jane Haruko LimaNeri, Darivaldo LuzSoares, Luana da SilvaGuerra, Sylvia de Fátima dos SantosOliveira, Darleise de SouzaFarias, Yasmin NascimentoGabbay, Yvone BenchimolLeite, José Paulo GagliardiLinhares, Alexandre da CostaMascarenhas, Joana D'Arc Pereira2018-07-19T14:08:15Z2018-07-19T14:08:15Z2012MAESTRI, Régis Piloni et al. Phylogenetic analysis of probable non-human genes of group A rotaviruses isolated from children with acute gastroenteritis in Belém, Brazil. Journal of Medical Virology, v. 84, n. 12, p. 1993-2002, 2012.0146-6615https://patua.iec.gov.br/handle/iec/326810.1002/jmv.23364Rotaviruses (RVs) are the main cause of acute viral gastroenteritis in both humans and young animals of various species such as calves, horses, pigs, dogs, cats, and birds. The genetic diversity of RVs is related to a variety of evolutionary mechanisms, including point mutation, and genome reassortment. The objective of this study was to characterize molecularly genes that encode structural and nonstructural proteins in unusual RV strains. The clinical specimens selected for this study were obtained from children and newborn with RV gastroenteritis, who participated in research projects on viral gastroenteritis conducted at the Evandro Chagas Institute. Structural (VP1-VP4, VP6, and VP7) and nonstructural (NSP1-NSP6) genes were amplified from stool samples by the polymerase chain reaction and subsequently sequenced. Eight unusual RV strains isolated from children and newborn with gastroenteritis were studied. Reassortment between genes of animal origin were observed in 5/8 (62.5%) strains analyzed. These results demonstrate that, although rare, interspecies (animal-human) transmission of RVs occurs in nature, as observed in the present study in strains NB150, HSP034, HSP180, HST327, and RV10109. This study is the first to be conducted in the Amazon region and supports previous data showing a close relationship between genes of human and animal origin, representing a challenge to the large-scale introduction of RV vaccines in national immunization programs.National Council for Scientific and Technological Development (CNPq), Brasília, Federal District, Brazil. Grant Number: 4742742008‐0Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Department of Virology. Rio de Janeiro, RJ, Brazil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Department of Virology. Rio de Janeiro, RJ, Brazil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.engWileyPhylogenetic analysis of probable non-human genes of group A rotaviruses isolated from children with acute gastroenteritis in Belém, Brazilinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleRotavirusInfecções por RotavirusDiarreia Infantil / virologiaGenéticainfo:eu-repo/semantics/openAccessreponame:Repositório Digital do Instituto Evandro Chagas (Patuá)instname:Instituto Evandro Chagas (IEC)instacron:IECORIGINALPhylogenetic analysis of probable non-human genes of group A rotaviruses isolated from children with acute gastroenteritis in Belém, Brazil.pdfPhylogenetic analysis of probable non-human genes of group A rotaviruses isolated from children with acute gastroenteritis in Belém, Brazil.pdfapplication/pdf181782https://patua.iec.gov.br/bitstreams/1a577e47-d8bb-480a-918b-d2fd394f27ce/download5d27e35efaa0b7d8dd13f3f14a32e252MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-871https://patua.iec.gov.br/bitstreams/552fc1c6-ab5f-4396-b1c4-5f8070e3f757/download52f1732ea66fbd1123abe39f5373b797MD52TEXTPhylogenetic analysis of probable non-human genes of group A rotaviruses isolated from children with acute gastroenteritis in Belém, Brazil.pdf.txtPhylogenetic analysis of probable non-human genes of group A rotaviruses isolated from children with acute gastroenteritis in Belém, Brazil.pdf.txtExtracted texttext/plain48969https://patua.iec.gov.br/bitstreams/59914d06-850a-4d36-8b19-6cb7dc22f81b/download08526cd2dde762ef036a7c1d3882137aMD55THUMBNAILPhylogenetic analysis of probable non-human genes of group A rotaviruses isolated from children with acute gastroenteritis in Belém, Brazil.pdf.jpgPhylogenetic analysis of probable non-human genes of group A rotaviruses isolated from children with acute gastroenteritis in Belém, Brazil.pdf.jpgGenerated Thumbnailimage/jpeg6521https://patua.iec.gov.br/bitstreams/10e31ce4-808d-4fc0-b277-d6b611b5ad25/download15f109805b1bfd140352e3edb20374c7MD56iec/32682022-10-20 21:09:11.716oai:patua.iec.gov.br:iec/3268https://patua.iec.gov.brRepositório InstitucionalPUBhttps://patua.iec.gov.br/oai/requestclariceneta@iec.gov.br || Biblioteca@iec.gov.bropendoar:2022-10-20T21:09:11Repositório Digital do Instituto Evandro Chagas (Patuá) - Instituto Evandro Chagas (IEC)falseVG9kb3Mgb3MgZG9jdW1lbnRvcyBkZXNzYSBjb2xlw6fDo28gc2VndWVtIGEgTGljZW7Dp2EgQ3JlYXRpdmUgY29tbW9ucy4= |
dc.title.pt_BR.fl_str_mv |
Phylogenetic analysis of probable non-human genes of group A rotaviruses isolated from children with acute gastroenteritis in Belém, Brazil |
title |
Phylogenetic analysis of probable non-human genes of group A rotaviruses isolated from children with acute gastroenteritis in Belém, Brazil |
spellingShingle |
Phylogenetic analysis of probable non-human genes of group A rotaviruses isolated from children with acute gastroenteritis in Belém, Brazil Maestri, Régis Piloni Rotavirus Infecções por Rotavirus Diarreia Infantil / virologia Genética |
title_short |
Phylogenetic analysis of probable non-human genes of group A rotaviruses isolated from children with acute gastroenteritis in Belém, Brazil |
title_full |
Phylogenetic analysis of probable non-human genes of group A rotaviruses isolated from children with acute gastroenteritis in Belém, Brazil |
title_fullStr |
Phylogenetic analysis of probable non-human genes of group A rotaviruses isolated from children with acute gastroenteritis in Belém, Brazil |
title_full_unstemmed |
Phylogenetic analysis of probable non-human genes of group A rotaviruses isolated from children with acute gastroenteritis in Belém, Brazil |
title_sort |
Phylogenetic analysis of probable non-human genes of group A rotaviruses isolated from children with acute gastroenteritis in Belém, Brazil |
author |
Maestri, Régis Piloni |
author_facet |
Maestri, Régis Piloni Kaiano, Jane Haruko Lima Neri, Darivaldo Luz Soares, Luana da Silva Guerra, Sylvia de Fátima dos Santos Oliveira, Darleise de Souza Farias, Yasmin Nascimento Gabbay, Yvone Benchimol Leite, José Paulo Gagliardi Linhares, Alexandre da Costa Mascarenhas, Joana D'Arc Pereira |
author_role |
author |
author2 |
Kaiano, Jane Haruko Lima Neri, Darivaldo Luz Soares, Luana da Silva Guerra, Sylvia de Fátima dos Santos Oliveira, Darleise de Souza Farias, Yasmin Nascimento Gabbay, Yvone Benchimol Leite, José Paulo Gagliardi Linhares, Alexandre da Costa Mascarenhas, Joana D'Arc Pereira |
author2_role |
author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Maestri, Régis Piloni Kaiano, Jane Haruko Lima Neri, Darivaldo Luz Soares, Luana da Silva Guerra, Sylvia de Fátima dos Santos Oliveira, Darleise de Souza Farias, Yasmin Nascimento Gabbay, Yvone Benchimol Leite, José Paulo Gagliardi Linhares, Alexandre da Costa Mascarenhas, Joana D'Arc Pereira |
dc.subject.decsPrimary.pt_BR.fl_str_mv |
Rotavirus Infecções por Rotavirus Diarreia Infantil / virologia Genética |
topic |
Rotavirus Infecções por Rotavirus Diarreia Infantil / virologia Genética |
description |
Rotaviruses (RVs) are the main cause of acute viral gastroenteritis in both humans and young animals of various species such as calves, horses, pigs, dogs, cats, and birds. The genetic diversity of RVs is related to a variety of evolutionary mechanisms, including point mutation, and genome reassortment. The objective of this study was to characterize molecularly genes that encode structural and nonstructural proteins in unusual RV strains. The clinical specimens selected for this study were obtained from children and newborn with RV gastroenteritis, who participated in research projects on viral gastroenteritis conducted at the Evandro Chagas Institute. Structural (VP1-VP4, VP6, and VP7) and nonstructural (NSP1-NSP6) genes were amplified from stool samples by the polymerase chain reaction and subsequently sequenced. Eight unusual RV strains isolated from children and newborn with gastroenteritis were studied. Reassortment between genes of animal origin were observed in 5/8 (62.5%) strains analyzed. These results demonstrate that, although rare, interspecies (animal-human) transmission of RVs occurs in nature, as observed in the present study in strains NB150, HSP034, HSP180, HST327, and RV10109. This study is the first to be conducted in the Amazon region and supports previous data showing a close relationship between genes of human and animal origin, representing a challenge to the large-scale introduction of RV vaccines in national immunization programs. |
publishDate |
2012 |
dc.date.issued.fl_str_mv |
2012 |
dc.date.accessioned.fl_str_mv |
2018-07-19T14:08:15Z |
dc.date.available.fl_str_mv |
2018-07-19T14:08:15Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
MAESTRI, Régis Piloni et al. Phylogenetic analysis of probable non-human genes of group A rotaviruses isolated from children with acute gastroenteritis in Belém, Brazil. Journal of Medical Virology, v. 84, n. 12, p. 1993-2002, 2012. |
dc.identifier.uri.fl_str_mv |
https://patua.iec.gov.br/handle/iec/3268 |
dc.identifier.issn.-.fl_str_mv |
0146-6615 |
dc.identifier.doi.-.fl_str_mv |
10.1002/jmv.23364 |
identifier_str_mv |
MAESTRI, Régis Piloni et al. Phylogenetic analysis of probable non-human genes of group A rotaviruses isolated from children with acute gastroenteritis in Belém, Brazil. Journal of Medical Virology, v. 84, n. 12, p. 1993-2002, 2012. 0146-6615 10.1002/jmv.23364 |
url |
https://patua.iec.gov.br/handle/iec/3268 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
Wiley |
publisher.none.fl_str_mv |
Wiley |
dc.source.none.fl_str_mv |
reponame:Repositório Digital do Instituto Evandro Chagas (Patuá) instname:Instituto Evandro Chagas (IEC) instacron:IEC |
instname_str |
Instituto Evandro Chagas (IEC) |
instacron_str |
IEC |
institution |
IEC |
reponame_str |
Repositório Digital do Instituto Evandro Chagas (Patuá) |
collection |
Repositório Digital do Instituto Evandro Chagas (Patuá) |
bitstream.url.fl_str_mv |
https://patua.iec.gov.br/bitstreams/1a577e47-d8bb-480a-918b-d2fd394f27ce/download https://patua.iec.gov.br/bitstreams/552fc1c6-ab5f-4396-b1c4-5f8070e3f757/download https://patua.iec.gov.br/bitstreams/59914d06-850a-4d36-8b19-6cb7dc22f81b/download https://patua.iec.gov.br/bitstreams/10e31ce4-808d-4fc0-b277-d6b611b5ad25/download |
bitstream.checksum.fl_str_mv |
5d27e35efaa0b7d8dd13f3f14a32e252 52f1732ea66fbd1123abe39f5373b797 08526cd2dde762ef036a7c1d3882137a 15f109805b1bfd140352e3edb20374c7 |
bitstream.checksumAlgorithm.fl_str_mv |
MD5 MD5 MD5 MD5 |
repository.name.fl_str_mv |
Repositório Digital do Instituto Evandro Chagas (Patuá) - Instituto Evandro Chagas (IEC) |
repository.mail.fl_str_mv |
clariceneta@iec.gov.br || Biblioteca@iec.gov.br |
_version_ |
1787533055906283520 |