A Zika virus from America is more efficiently transmitted than an Asian virus by Aedes aegypti mosquitoes from Asia

• Autor(a) principal: Pompon, Julien
• Data de Publicação: 2017
• Outros Autores: Morales-Vargas, Ronald, Manuel, Menchie, Tan, Cheong Huat, Vial, Thomas, Tan, Jun Hao, Sessions, October M, Vasconcelos, Pedro Fernando da Costa, Ng, Lee Ching, Missé, Dorothée
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Digital do Instituto Evandro Chagas (Patuá)
• DOI: 10.1038/s41598-017-01282-6
• Texto Completo: https://patua.iec.gov.br/handle/iec/2830
• topic: Aedes / patogenicidade; Aedes / virologia; Zika virus / isolamento & purificação; Zika virus / genética; Zika virus / patogenicidade; Infecção pelo Zika virus / transmissão; Infecção pelo Zika virus / virologia; Insetos Vetores / virologia; Vetores de Doenças / classificação; Genoma Viral; Filogenia; Entomologia; Sequência de Bases; Reação em Cadeia da Polimerase em Tempo Real / métodos; Reação em Cadeia da Polimerase Via Transcriptase Reversa / métodos; Américas; Singapura; Ásia Sudeste

Zika is a mosquito-borne disease associated with neurological disorders that causes an on-going pandemic. The first outbreak was recorded in Micronesia in 2007, then in French Polynesia in 2014 from which it spread to South America in 2015 and ignited a widespread epidemic. Interestingly, Zika outbreaks in Asia remained of moderate intensity although the virus is circulating. To understand these epidemiological variations, we investigated the entomological determinants of ZIKV transmission in Asia. We used oral infection of mosquitoes collected in Singapore to identify the vector species, to quantify the blood infection threshold and to compare transmissibility between an Asian ZIKV strain (H/PF13) and an American strain collected in Brazil (BE H 815744). We have confirmed the vector status of Aedes aegypti and determined that 103pfu/ml of blood is sufficient to infect mosquitoes. We showed that only the American strain was present in the saliva 3 days post-infection, and that this strain had a 30-40% higher rate of saliva infection in Ae. aegypti from 3 to 14 days post-infection than the Asian strain. Our data suggests that American strains are more efficiently transmitted than Asian strains, which raises concerns about the introduction of American strains in Asia.