Genomic organization of repetitive DNA in woodpeckers (Aves, Piciformes): implications for karyotype and ZW sex chromosome differentiation
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Digital do Instituto Evandro Chagas (Patuá) |
Texto Completo: | https://patua.iec.gov.br/handle/iec/2524 |
Resumo: | Birds are characterized by a low proportion of repetitive DNA in their genome when compared to other vertebrates. Among birds, species belonging to Piciformes order, such as woodpeckers, show a relatively higher amount of these sequences. The aim of this study was to analyze the distribution of different classes of repetitive DNAÐincluding microsatellites, telomere sequences and 18S rDNAÐin the karyotype of three Picidae species (Aves, Piciformes)ÐColaptes melanochloros (2n = 84), Colaptes campestris (2n = 84) and Melanerpes candidus (2n = 64)±by means of fluorescence in situ hybridization. Clusters of 18S rDNA were found in one microchromosome pair in each of the three species, coinciding to a region of (CGG)10 sequence accumulation. Interstitial telomeric sequences were found in some macrochromosomes pairs, indicating possible regions of fusions, which can be related to variation of diploid number in the family. Only one, from the 11 different microsatellite sequences used, did not produce any signals. Both species of genus Colaptes showed a similar distribution of microsatellite sequences, with some difference when compared to M. candidus. Microsatellites were found preferentially in the centromeric and telomeric regions of micro and macrochromosomes. However, some sequences produced patterns of interstitial bands in the Z chromosome, which corresponds to the largest element of the karyotype in all three species. This was not observed in theWchromosome of Colaptes melanochloros, which is heterochromatic in most of its length, but was not hybridized by any of the sequences used. These results highlight the importance of microsatellite sequences in differentiation of sex chromosomes, and the accumulation of these sequences is probably responsible for the enlargement of the Z chromosome. |
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Oliveira, Thays Duarte deKretschmer, RafaelBertocchi, Natasha AvilaDegrandi, Tiago MarafigaOliveira, Edivaldo Herculano Correa deCioffi, Marcelo de BelloGarnero, Analía del ValleGunski, Ricardo José2017-02-15T18:09:57Z2017-02-15T18:09:57Z201720170215OLIVEIRA, Thays Duarte de et al. Genomic organization of repetitive DNA in woodpeckers (Aves, Piciformes): implications for karyotype and ZW sex chromosome differentiation. PLoS ONE, v. 12, n. 1, 2017.1932-6203https://patua.iec.gov.br/handle/iec/252410.1371/journal.pone.0169987Birds are characterized by a low proportion of repetitive DNA in their genome when compared to other vertebrates. Among birds, species belonging to Piciformes order, such as woodpeckers, show a relatively higher amount of these sequences. The aim of this study was to analyze the distribution of different classes of repetitive DNAÐincluding microsatellites, telomere sequences and 18S rDNAÐin the karyotype of three Picidae species (Aves, Piciformes)ÐColaptes melanochloros (2n = 84), Colaptes campestris (2n = 84) and Melanerpes candidus (2n = 64)±by means of fluorescence in situ hybridization. Clusters of 18S rDNA were found in one microchromosome pair in each of the three species, coinciding to a region of (CGG)10 sequence accumulation. Interstitial telomeric sequences were found in some macrochromosomes pairs, indicating possible regions of fusions, which can be related to variation of diploid number in the family. Only one, from the 11 different microsatellite sequences used, did not produce any signals. Both species of genus Colaptes showed a similar distribution of microsatellite sequences, with some difference when compared to M. candidus. Microsatellites were found preferentially in the centromeric and telomeric regions of micro and macrochromosomes. However, some sequences produced patterns of interstitial bands in the Z chromosome, which corresponds to the largest element of the karyotype in all three species. This was not observed in theWchromosome of Colaptes melanochloros, which is heterochromatic in most of its length, but was not hybridized by any of the sequences used. These results highlight the importance of microsatellite sequences in differentiation of sex chromosomes, and the accumulation of these sequences is probably responsible for the enlargement of the Z chromosome.Universidade Federal do Pampa. Programa de Pós-Graduação em Ciências Biológicas. São Gabriel, RS, Brazil.Universidade Federal do Rio Grande do Sul. Programa de Pós-Graduação em Genética e Biologia Molecular. Porto Alegre, RS, Brazil.Universidade Federal do Pampa. Programa de Pós-Graduação em Ciências Biológicas. São Gabriel, RS, Brazil.Universidade Federal do Paraná. Programa de Pós-Graduação em Genética. Curitiba, PR, Brazil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil / Universidade Federal do Pará. Instituto de Ciências Exatas e Naturais, Belém, PA, Brazil.Universidade Federal de São Carlos. Departamento de Genética e Evolução. São Carlos, SP, BrazilUniversidade Federal do Pampa. Programa de Pós-Graduação em Ciências Biológicas. São Gabriel, RS, Brazil.engPublic Library of ScienceGenomic organization of repetitive DNA in woodpeckers (Aves, Piciformes): implications for karyotype and ZW sex chromosome differentiationinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleAves / anatomia & histologiaGenomaCariotipagemMapeamento CromossômicoCitogenética / métodosPiciformesinfo:eu-repo/semantics/openAccessreponame:Repositório Digital do Instituto Evandro Chagas (Patuá)instname:Instituto Evandro Chagas (IEC)instacron:IECTHUMBNAILGenomic organization of repetitive DNA in woodpeckers (Aves, Piciformes): implications for karyotype and ZW sex chromosome differentiation.pdf.jpgGenomic organization of repetitive DNA in woodpeckers (Aves, Piciformes): implications for karyotype and ZW sex chromosome differentiation.pdf.jpgGenerated Thumbnailimage/jpeg5932https://patua.iec.gov.br/bitstreams/b17657e4-ed67-4b58-9ad9-56ab95dfa2e8/downloade6acb1661a929b8dda40c31602d819ccMD57ORIGINALGenomic organization of repetitive DNA in woodpeckers (Aves, Piciformes): implications for karyotype and ZW sex chromosome differentiation.pdfGenomic organization of repetitive DNA in woodpeckers (Aves, Piciformes): implications for karyotype and ZW sex chromosome differentiation.pdfapplication/pdf1379578https://patua.iec.gov.br/bitstreams/a42c0404-9a30-45c7-a7b5-357856f5fc3a/download6aa546aa6d1c098f261848dce328bf1bMD51LICENSElicense.txtlicense.txttext/plain; charset=utf-871https://patua.iec.gov.br/bitstreams/75d029d2-9421-416f-9db5-a49d627f9d96/download52f1732ea66fbd1123abe39f5373b797MD52TEXTOLIVEIRApone.0169987.pdf.txtOLIVEIRApone.0169987.pdf.txtExtracted texttext/plain31376https://patua.iec.gov.br/bitstreams/d5145e3d-cebe-4047-9e41-3cd03d9abb4f/download8bc6ed4fb4c0b5e4b78f343c6208f638MD53Genomic organization of repetitive DNA in woodpeckers (Aves, Piciformes): implications for karyotype and ZW sex chromosome differentiation.pdf.txtGenomic organization of repetitive DNA in woodpeckers (Aves, Piciformes): implications for karyotype and ZW sex chromosome differentiation.pdf.txtExtracted texttext/plain33910https://patua.iec.gov.br/bitstreams/4cfc7345-40e7-434a-8a95-8feaf70a8fa9/download4636b6cd70d4a8a5e8bc3ccb4521f6d1MD56iec/25242022-10-21 00:45:36.413oai:patua.iec.gov.br:iec/2524https://patua.iec.gov.brRepositório InstitucionalPUBhttps://patua.iec.gov.br/oai/requestclariceneta@iec.gov.br || Biblioteca@iec.gov.bropendoar:2022-10-21T00:45:36Repositório Digital do Instituto Evandro Chagas (Patuá) - Instituto Evandro Chagas (IEC)falseVG9kb3Mgb3MgZG9jdW1lbnRvcyBkZXNzYSBjb2xlw6fDo28gc2VndWVtIGEgTGljZW7Dp2EgQ3JlYXRpdmUgY29tbW9ucy4= |
dc.title.pt_BR.fl_str_mv |
Genomic organization of repetitive DNA in woodpeckers (Aves, Piciformes): implications for karyotype and ZW sex chromosome differentiation |
title |
Genomic organization of repetitive DNA in woodpeckers (Aves, Piciformes): implications for karyotype and ZW sex chromosome differentiation |
spellingShingle |
Genomic organization of repetitive DNA in woodpeckers (Aves, Piciformes): implications for karyotype and ZW sex chromosome differentiation Oliveira, Thays Duarte de Aves / anatomia & histologia Genoma Cariotipagem Mapeamento Cromossômico Citogenética / métodos Piciformes |
title_short |
Genomic organization of repetitive DNA in woodpeckers (Aves, Piciformes): implications for karyotype and ZW sex chromosome differentiation |
title_full |
Genomic organization of repetitive DNA in woodpeckers (Aves, Piciformes): implications for karyotype and ZW sex chromosome differentiation |
title_fullStr |
Genomic organization of repetitive DNA in woodpeckers (Aves, Piciformes): implications for karyotype and ZW sex chromosome differentiation |
title_full_unstemmed |
Genomic organization of repetitive DNA in woodpeckers (Aves, Piciformes): implications for karyotype and ZW sex chromosome differentiation |
title_sort |
Genomic organization of repetitive DNA in woodpeckers (Aves, Piciformes): implications for karyotype and ZW sex chromosome differentiation |
author |
Oliveira, Thays Duarte de |
author_facet |
Oliveira, Thays Duarte de Kretschmer, Rafael Bertocchi, Natasha Avila Degrandi, Tiago Marafiga Oliveira, Edivaldo Herculano Correa de Cioffi, Marcelo de Bello Garnero, Analía del Valle Gunski, Ricardo José |
author_role |
author |
author2 |
Kretschmer, Rafael Bertocchi, Natasha Avila Degrandi, Tiago Marafiga Oliveira, Edivaldo Herculano Correa de Cioffi, Marcelo de Bello Garnero, Analía del Valle Gunski, Ricardo José |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Oliveira, Thays Duarte de Kretschmer, Rafael Bertocchi, Natasha Avila Degrandi, Tiago Marafiga Oliveira, Edivaldo Herculano Correa de Cioffi, Marcelo de Bello Garnero, Analía del Valle Gunski, Ricardo José |
dc.subject.decsPrimary.pt_BR.fl_str_mv |
Aves / anatomia & histologia Genoma Cariotipagem Mapeamento Cromossômico Citogenética / métodos Piciformes |
topic |
Aves / anatomia & histologia Genoma Cariotipagem Mapeamento Cromossômico Citogenética / métodos Piciformes |
description |
Birds are characterized by a low proportion of repetitive DNA in their genome when compared to other vertebrates. Among birds, species belonging to Piciformes order, such as woodpeckers, show a relatively higher amount of these sequences. The aim of this study was to analyze the distribution of different classes of repetitive DNAÐincluding microsatellites, telomere sequences and 18S rDNAÐin the karyotype of three Picidae species (Aves, Piciformes)ÐColaptes melanochloros (2n = 84), Colaptes campestris (2n = 84) and Melanerpes candidus (2n = 64)±by means of fluorescence in situ hybridization. Clusters of 18S rDNA were found in one microchromosome pair in each of the three species, coinciding to a region of (CGG)10 sequence accumulation. Interstitial telomeric sequences were found in some macrochromosomes pairs, indicating possible regions of fusions, which can be related to variation of diploid number in the family. Only one, from the 11 different microsatellite sequences used, did not produce any signals. Both species of genus Colaptes showed a similar distribution of microsatellite sequences, with some difference when compared to M. candidus. Microsatellites were found preferentially in the centromeric and telomeric regions of micro and macrochromosomes. However, some sequences produced patterns of interstitial bands in the Z chromosome, which corresponds to the largest element of the karyotype in all three species. This was not observed in theWchromosome of Colaptes melanochloros, which is heterochromatic in most of its length, but was not hybridized by any of the sequences used. These results highlight the importance of microsatellite sequences in differentiation of sex chromosomes, and the accumulation of these sequences is probably responsible for the enlargement of the Z chromosome. |
publishDate |
2017 |
dc.date.iso.-.fl_str_mv |
20170215 |
dc.date.accessioned.fl_str_mv |
2017-02-15T18:09:57Z |
dc.date.available.fl_str_mv |
2017-02-15T18:09:57Z |
dc.date.issued.fl_str_mv |
2017 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
OLIVEIRA, Thays Duarte de et al. Genomic organization of repetitive DNA in woodpeckers (Aves, Piciformes): implications for karyotype and ZW sex chromosome differentiation. PLoS ONE, v. 12, n. 1, 2017. |
dc.identifier.uri.fl_str_mv |
https://patua.iec.gov.br/handle/iec/2524 |
dc.identifier.issn.-.fl_str_mv |
1932-6203 |
dc.identifier.doi.-.fl_str_mv |
10.1371/journal.pone.0169987 |
identifier_str_mv |
OLIVEIRA, Thays Duarte de et al. Genomic organization of repetitive DNA in woodpeckers (Aves, Piciformes): implications for karyotype and ZW sex chromosome differentiation. PLoS ONE, v. 12, n. 1, 2017. 1932-6203 10.1371/journal.pone.0169987 |
url |
https://patua.iec.gov.br/handle/iec/2524 |
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eng |
language |
eng |
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info:eu-repo/semantics/openAccess |
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openAccess |
dc.publisher.none.fl_str_mv |
Public Library of Science |
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Public Library of Science |
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IEC |
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