Use of recombinant antigens for sensitive serodiagnosis of american tegumentary leishmaniasis caused by different leishmania species

• Autor(a) principal: Sato, Camila Massae
• Data de Publicação: 2017
• Outros Autores: Sanchez, Maria Carmen Arroyo, Celeste, Beatriz Julieta, Duthie, Malcolm S, Guderian, Jeffrey, Reed, Steven G, Brito, Maria Edileuza Felinto de, Campos, Marliane Batista, Encarnação, Helia Valeria de Souza, Guerra, Jorge, Mesquita, Tirza Gabrielle Ramos de, Pinheiro, Suzana Kanawati, Ramasawmy, Rajendranath, Silveira, Fernando Tobias, Souza, Marina de Assis, Goto, Hiro
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Digital do Instituto Evandro Chagas (Patuá)
• Texto Completo: https://patua.iec.gov.br/handle/iec/2863
• topic: Anticorpos Antiprotozoários / sangue; Anticorpos Antiprotozoários / genética; Anticorpos Antiprotozoários / imunologia; Leishmania / imunologia; Leishmaniose Cutânea / diagnóstico; Proteínas Recombinantes / genética; Proteínas Recombinantes / imunologia; Sensibilidade e Especificidade; Testes Sorológicos / métodos; Ensaio de Imunoadsorção Enzimática / métodos

American tegumentary leishmaniasis (ATL) (also known as cutaneous leishmaniasis [CL]) is caused by various species of protozoa of the genus Leishmania. The diagnosis is achieved on a clinical, epidemiological, and pathological basis, supported by positive parasitological exams and demonstration of leishmanin delayedtype hypersensitivity. Serological assays are not routinely used in the diagnosis because many are considered to have low sensitivity and the particular Leishmania species causing the disease can lead to variable performance. In the present study, we generated recombinant versions of two highly conserved Leishmania proteins, Leishmania (Viannia) braziliensis-derived Lb8E and Lb6H, and evaluated both in enzyme-linked immunosorbent assays (ELISA). Recombinant Lb6H (rLb6H) had better performance and reacted with 100.0% of the ATL and 89.4% of the VL samples. These reactions with rLb6H were highly specific (98.5%) when compared against those for samples from healthy control individuals. We then assessed rLb6H against sera from ATL patients infected with different species of Leishmania prevalent in Brazil [Leishmania (Leishmania) amazonensis, L. (Viannia) braziliensis, and L. (V.) guyanensis] and samples from patients with other infectious diseases. In analyses of 500 sera, ELISA using rLb6H detected all 219 ATL samples (sensitivity of 100.0%) with an overall specificity of 93.9% (considering healthy individuals and other infectious diseases patients). Only a minority of samples from Chagas disease patients possessed antibodies against rLb6H, and all of these responses were low (with a highest reactivity index of 2.2). Taken together, our data support further evaluation of rLb6H and the potential for its routine use in the serological diagnosis of ATL.