Analysis of cytokine profile and growth factors in platelet-rich plasma obtained by open systems and commercial columns
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Einstein (São Paulo) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1679-45082016000300391 |
Resumo: | ABSTRACT Objective: To evaluate growth factors and cytokines in samples of platelet-rich plasma obtained by three different centrifugation methods. Methods: Peripheral blood of six individuals with no hematological diseases, aged 18 to 68 years, was drawn to obtain platelet-rich plasma, using the open method and commercial columns by Medtronic and Biomet. The products obtained with the different types of centrifugation were submitted to laboratory analysis, including pro-inflammatory cytokines and chemokines by flow cytometry assays, the concentration of fibroblast growth factors-2 (FGF-2) and transforming growth factor-beta1 (TGF-β1). Results: The diverse separation methods generated systematically different profiles regarding number of platelets and leukocytes. The Medtronic system yielded a product with the highest concentration of platelets, and the open method, with the lowest concentration of platelets. The results of cytokine analysis showed that the different types of centrifugation yielded products with high concentrations of interleukin 8, interleukin 1β. The open system resulted in a product with high levels of interleukin 6. Other cytokines and chemokines measured were similar between systems. The product obtained with the open method showed higher levels of TGF-β1 in relation to other systems and low FGF-2 levels. Conclusion: The formed elements, growth factors and cytokines in samples of platelet-rich plasma varied according to the centrifugation technique used. |
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Analysis of cytokine profile and growth factors in platelet-rich plasma obtained by open systems and commercial columnsPlatelet-rich plasmaCytokinesIntercellular signaling peptides and proteinsCentrifugation/methodsABSTRACT Objective: To evaluate growth factors and cytokines in samples of platelet-rich plasma obtained by three different centrifugation methods. Methods: Peripheral blood of six individuals with no hematological diseases, aged 18 to 68 years, was drawn to obtain platelet-rich plasma, using the open method and commercial columns by Medtronic and Biomet. The products obtained with the different types of centrifugation were submitted to laboratory analysis, including pro-inflammatory cytokines and chemokines by flow cytometry assays, the concentration of fibroblast growth factors-2 (FGF-2) and transforming growth factor-beta1 (TGF-β1). Results: The diverse separation methods generated systematically different profiles regarding number of platelets and leukocytes. The Medtronic system yielded a product with the highest concentration of platelets, and the open method, with the lowest concentration of platelets. The results of cytokine analysis showed that the different types of centrifugation yielded products with high concentrations of interleukin 8, interleukin 1β. The open system resulted in a product with high levels of interleukin 6. Other cytokines and chemokines measured were similar between systems. The product obtained with the open method showed higher levels of TGF-β1 in relation to other systems and low FGF-2 levels. Conclusion: The formed elements, growth factors and cytokines in samples of platelet-rich plasma varied according to the centrifugation technique used.Instituto Israelita de Ensino e Pesquisa Albert Einstein2016-09-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1679-45082016000300391einstein (São Paulo) v.14 n.3 2016reponame:Einstein (São Paulo)instname:Instituto Israelita de Ensino e Pesquisa Albert Einstein (IIEPAE)instacron:IIEPAE10.1590/S1679-45082016AO3548info:eu-repo/semantics/openAccessPochini,Alberto de CastroAntonioli,ElianeBucci,Daniella ZanettiSardinha,Luiz RobertoAndreoli,Carlos VicenteFerretti,MarioEjnisman,BennoGoldberg,Anna CarlaCohen,Moiséseng2016-10-14T00:00:00Zoai:scielo:S1679-45082016000300391Revistahttps://journal.einstein.br/pt-br/ONGhttps://old.scielo.br/oai/scielo-oai.php||revista@einstein.br2317-63851679-4508opendoar:2016-10-14T00:00Einstein (São Paulo) - Instituto Israelita de Ensino e Pesquisa Albert Einstein (IIEPAE)false |
dc.title.none.fl_str_mv |
Analysis of cytokine profile and growth factors in platelet-rich plasma obtained by open systems and commercial columns |
title |
Analysis of cytokine profile and growth factors in platelet-rich plasma obtained by open systems and commercial columns |
spellingShingle |
Analysis of cytokine profile and growth factors in platelet-rich plasma obtained by open systems and commercial columns Pochini,Alberto de Castro Platelet-rich plasma Cytokines Intercellular signaling peptides and proteins Centrifugation/methods |
title_short |
Analysis of cytokine profile and growth factors in platelet-rich plasma obtained by open systems and commercial columns |
title_full |
Analysis of cytokine profile and growth factors in platelet-rich plasma obtained by open systems and commercial columns |
title_fullStr |
Analysis of cytokine profile and growth factors in platelet-rich plasma obtained by open systems and commercial columns |
title_full_unstemmed |
Analysis of cytokine profile and growth factors in platelet-rich plasma obtained by open systems and commercial columns |
title_sort |
Analysis of cytokine profile and growth factors in platelet-rich plasma obtained by open systems and commercial columns |
author |
Pochini,Alberto de Castro |
author_facet |
Pochini,Alberto de Castro Antonioli,Eliane Bucci,Daniella Zanetti Sardinha,Luiz Roberto Andreoli,Carlos Vicente Ferretti,Mario Ejnisman,Benno Goldberg,Anna Carla Cohen,Moisés |
author_role |
author |
author2 |
Antonioli,Eliane Bucci,Daniella Zanetti Sardinha,Luiz Roberto Andreoli,Carlos Vicente Ferretti,Mario Ejnisman,Benno Goldberg,Anna Carla Cohen,Moisés |
author2_role |
author author author author author author author author |
dc.contributor.author.fl_str_mv |
Pochini,Alberto de Castro Antonioli,Eliane Bucci,Daniella Zanetti Sardinha,Luiz Roberto Andreoli,Carlos Vicente Ferretti,Mario Ejnisman,Benno Goldberg,Anna Carla Cohen,Moisés |
dc.subject.por.fl_str_mv |
Platelet-rich plasma Cytokines Intercellular signaling peptides and proteins Centrifugation/methods |
topic |
Platelet-rich plasma Cytokines Intercellular signaling peptides and proteins Centrifugation/methods |
description |
ABSTRACT Objective: To evaluate growth factors and cytokines in samples of platelet-rich plasma obtained by three different centrifugation methods. Methods: Peripheral blood of six individuals with no hematological diseases, aged 18 to 68 years, was drawn to obtain platelet-rich plasma, using the open method and commercial columns by Medtronic and Biomet. The products obtained with the different types of centrifugation were submitted to laboratory analysis, including pro-inflammatory cytokines and chemokines by flow cytometry assays, the concentration of fibroblast growth factors-2 (FGF-2) and transforming growth factor-beta1 (TGF-β1). Results: The diverse separation methods generated systematically different profiles regarding number of platelets and leukocytes. The Medtronic system yielded a product with the highest concentration of platelets, and the open method, with the lowest concentration of platelets. The results of cytokine analysis showed that the different types of centrifugation yielded products with high concentrations of interleukin 8, interleukin 1β. The open system resulted in a product with high levels of interleukin 6. Other cytokines and chemokines measured were similar between systems. The product obtained with the open method showed higher levels of TGF-β1 in relation to other systems and low FGF-2 levels. Conclusion: The formed elements, growth factors and cytokines in samples of platelet-rich plasma varied according to the centrifugation technique used. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-09-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1679-45082016000300391 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1679-45082016000300391 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1679-45082016AO3548 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Instituto Israelita de Ensino e Pesquisa Albert Einstein |
publisher.none.fl_str_mv |
Instituto Israelita de Ensino e Pesquisa Albert Einstein |
dc.source.none.fl_str_mv |
einstein (São Paulo) v.14 n.3 2016 reponame:Einstein (São Paulo) instname:Instituto Israelita de Ensino e Pesquisa Albert Einstein (IIEPAE) instacron:IIEPAE |
instname_str |
Instituto Israelita de Ensino e Pesquisa Albert Einstein (IIEPAE) |
instacron_str |
IIEPAE |
institution |
IIEPAE |
reponame_str |
Einstein (São Paulo) |
collection |
Einstein (São Paulo) |
repository.name.fl_str_mv |
Einstein (São Paulo) - Instituto Israelita de Ensino e Pesquisa Albert Einstein (IIEPAE) |
repository.mail.fl_str_mv |
||revista@einstein.br |
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1752129908419592192 |