Development of a Device to Obtain Platelet-Rich Plasma (PRP)

Detalhes bibliográficos
Autor(a) principal: Mota,Marília de Lima
Data de Publicação: 2022
Outros Autores: Barreto,Ronald Bispo, Leite,Bárbara Ramos, Cavalcante,Beatriz Costa Cardoso Dourado
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Revista Brasileira de Ortopedia (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-36162022000200289
Resumo: Abstract Objective To present an innovative device that applies the double centrifugation method to obtain platelet-rich plasma (PRP), assessing whether there was an effective increase in the concentration of platelets. Method Ten volunteers underwent blood collection. The samples were separated in 20 ml syringes, sealed and subjected to the double centrifugation protocol at 1,100 revolutions per minute (rpm) for 15 minutes, resulting in the separation of red blood cells, plasma with platelets, and leukocytes. Then, 10 ml syringes were added to remove 9 ml, respecting the “buffy coat” parameter, collecting 8 ml above and 1 ml below for the second centrifugation and transferring again to the 20 ml syringe. The plasma was again centrifuged at 1,550 rpm for 10 minutes; as a result, it was divided into two parts: at the top, consisting of low platelet plasma (LPP), and at the bottom, by the platelet button. Part of the LPP was discarded, leaving only 3 ml with the platelet button. The cells were then counted. Results This innovative device was able to increase the concentration of platelets by almost three times compared with the baseline. In addition, the preparation time for the PRP was adequate, lasting only 35 to 40 minutes. Conclusions Platelet-rich plasma was successfully obtained by the double centrifuge protocol, allowing its clinical use. In addition, obtaining through the presented device promotes greater applicability in the preparation of PRP in specific centers, furthermore, being a quick and economical way to obtain PRP.
id SBOT-2_77900ab6eeb3bbee6757a59b4a8fe65d
oai_identifier_str oai:scielo:S0102-36162022000200289
network_acronym_str SBOT-2
network_name_str Revista Brasileira de Ortopedia (Online)
repository_id_str
spelling Development of a Device to Obtain Platelet-Rich Plasma (PRP)deviceplateletplatelet-rich plasmaAbstract Objective To present an innovative device that applies the double centrifugation method to obtain platelet-rich plasma (PRP), assessing whether there was an effective increase in the concentration of platelets. Method Ten volunteers underwent blood collection. The samples were separated in 20 ml syringes, sealed and subjected to the double centrifugation protocol at 1,100 revolutions per minute (rpm) for 15 minutes, resulting in the separation of red blood cells, plasma with platelets, and leukocytes. Then, 10 ml syringes were added to remove 9 ml, respecting the “buffy coat” parameter, collecting 8 ml above and 1 ml below for the second centrifugation and transferring again to the 20 ml syringe. The plasma was again centrifuged at 1,550 rpm for 10 minutes; as a result, it was divided into two parts: at the top, consisting of low platelet plasma (LPP), and at the bottom, by the platelet button. Part of the LPP was discarded, leaving only 3 ml with the platelet button. The cells were then counted. Results This innovative device was able to increase the concentration of platelets by almost three times compared with the baseline. In addition, the preparation time for the PRP was adequate, lasting only 35 to 40 minutes. Conclusions Platelet-rich plasma was successfully obtained by the double centrifuge protocol, allowing its clinical use. In addition, obtaining through the presented device promotes greater applicability in the preparation of PRP in specific centers, furthermore, being a quick and economical way to obtain PRP.Sociedade Brasileira de Ortopedia e Traumatologia2022-04-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-36162022000200289Revista Brasileira de Ortopedia v.57 n.2 2022reponame:Revista Brasileira de Ortopedia (Online)instname:Sociedade Brasileira de Ortopedia e Traumatologia (SBOT)instacron:SBOT10.1055/s-0040-1721835info:eu-repo/semantics/openAccessMota,Marília de LimaBarreto,Ronald BispoLeite,Bárbara RamosCavalcante,Beatriz Costa Cardoso Douradoeng2022-07-04T00:00:00Zoai:scielo:S0102-36162022000200289Revistahttp://www.rbo.org.br/https://old.scielo.br/oai/scielo-oai.php||rbo@sbot.org.br1982-43780102-3616opendoar:2022-07-04T00:00Revista Brasileira de Ortopedia (Online) - Sociedade Brasileira de Ortopedia e Traumatologia (SBOT)false
dc.title.none.fl_str_mv Development of a Device to Obtain Platelet-Rich Plasma (PRP)
title Development of a Device to Obtain Platelet-Rich Plasma (PRP)
spellingShingle Development of a Device to Obtain Platelet-Rich Plasma (PRP)
Mota,Marília de Lima
device
platelet
platelet-rich plasma
title_short Development of a Device to Obtain Platelet-Rich Plasma (PRP)
title_full Development of a Device to Obtain Platelet-Rich Plasma (PRP)
title_fullStr Development of a Device to Obtain Platelet-Rich Plasma (PRP)
title_full_unstemmed Development of a Device to Obtain Platelet-Rich Plasma (PRP)
title_sort Development of a Device to Obtain Platelet-Rich Plasma (PRP)
author Mota,Marília de Lima
author_facet Mota,Marília de Lima
Barreto,Ronald Bispo
Leite,Bárbara Ramos
Cavalcante,Beatriz Costa Cardoso Dourado
author_role author
author2 Barreto,Ronald Bispo
Leite,Bárbara Ramos
Cavalcante,Beatriz Costa Cardoso Dourado
author2_role author
author
author
dc.contributor.author.fl_str_mv Mota,Marília de Lima
Barreto,Ronald Bispo
Leite,Bárbara Ramos
Cavalcante,Beatriz Costa Cardoso Dourado
dc.subject.por.fl_str_mv device
platelet
platelet-rich plasma
topic device
platelet
platelet-rich plasma
description Abstract Objective To present an innovative device that applies the double centrifugation method to obtain platelet-rich plasma (PRP), assessing whether there was an effective increase in the concentration of platelets. Method Ten volunteers underwent blood collection. The samples were separated in 20 ml syringes, sealed and subjected to the double centrifugation protocol at 1,100 revolutions per minute (rpm) for 15 minutes, resulting in the separation of red blood cells, plasma with platelets, and leukocytes. Then, 10 ml syringes were added to remove 9 ml, respecting the “buffy coat” parameter, collecting 8 ml above and 1 ml below for the second centrifugation and transferring again to the 20 ml syringe. The plasma was again centrifuged at 1,550 rpm for 10 minutes; as a result, it was divided into two parts: at the top, consisting of low platelet plasma (LPP), and at the bottom, by the platelet button. Part of the LPP was discarded, leaving only 3 ml with the platelet button. The cells were then counted. Results This innovative device was able to increase the concentration of platelets by almost three times compared with the baseline. In addition, the preparation time for the PRP was adequate, lasting only 35 to 40 minutes. Conclusions Platelet-rich plasma was successfully obtained by the double centrifuge protocol, allowing its clinical use. In addition, obtaining through the presented device promotes greater applicability in the preparation of PRP in specific centers, furthermore, being a quick and economical way to obtain PRP.
publishDate 2022
dc.date.none.fl_str_mv 2022-04-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-36162022000200289
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-36162022000200289
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1055/s-0040-1721835
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Ortopedia e Traumatologia
publisher.none.fl_str_mv Sociedade Brasileira de Ortopedia e Traumatologia
dc.source.none.fl_str_mv Revista Brasileira de Ortopedia v.57 n.2 2022
reponame:Revista Brasileira de Ortopedia (Online)
instname:Sociedade Brasileira de Ortopedia e Traumatologia (SBOT)
instacron:SBOT
instname_str Sociedade Brasileira de Ortopedia e Traumatologia (SBOT)
instacron_str SBOT
institution SBOT
reponame_str Revista Brasileira de Ortopedia (Online)
collection Revista Brasileira de Ortopedia (Online)
repository.name.fl_str_mv Revista Brasileira de Ortopedia (Online) - Sociedade Brasileira de Ortopedia e Traumatologia (SBOT)
repository.mail.fl_str_mv ||rbo@sbot.org.br
_version_ 1752122363314438144