Suscetibilidade de Biomphalaria tenagophila e Biomphalaria straminea a infecção por Schistosoma mansoni detectada pela reação em cadeia da polimerase em baixa estringência

• Autor(a) principal: JANNOTTI-PASSOS, Liana Konovaloff
• Data de Publicação: 2000
• Outros Autores: SOUZA, Cecilia Pereira de
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Revista do Instituto de Medicina Tropical de São Paulo
• Texto Completo: https://www.revistas.usp.br/rimtsp/article/view/30458
• topic: Schistosoma mans; Biomphalaria tenagoph; Biomphalaria strami; Susceptibil; LS-PCR techni

In order to determine Schistosoma mansoni infection rates in Biomphalaria tenagophila and B. straminea, low stringency polymerase chain reaction (LS-PCR) technique was used as a complementary method to light exposure technique. LS-PCR has already been standardized in our laboratory to detect the trematode DNA in B. glabrata. Higher S. mansoni infection rates were detected using conventional method and LS-PCR. The parasite DNA profile was detected in both species after 7-day exposure to miracidia, using LS-PCR. This technique enables early detection of schistosomiasis transmission focuses, in endemic areas, before the beginning of cercariae shedding.